Job ID = 6527813 SRX = SRX287717 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:45:11 prefetch.2.10.7: 1) Downloading 'SRR869906'... 2020-06-29T13:45:11 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:49:12 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:49:12 prefetch.2.10.7: 1) 'SRR869906' was downloaded successfully Read 17419333 spots for SRR869906/SRR869906.sra Written 17419333 spots for SRR869906/SRR869906.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:32 17419333 reads; of these: 17419333 (100.00%) were unpaired; of these: 1038235 (5.96%) aligned 0 times 13169771 (75.60%) aligned exactly 1 time 3211327 (18.44%) aligned >1 times 94.04% overall alignment rate Time searching: 00:05:32 Overall time: 00:05:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1215652 / 16381098 = 0.0742 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:04:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:04:56: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:04:56: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:05:02: 1000000 INFO @ Mon, 29 Jun 2020 23:05:08: 2000000 INFO @ Mon, 29 Jun 2020 23:05:14: 3000000 INFO @ Mon, 29 Jun 2020 23:05:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:05:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:05:26: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:05:26: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:05:27: 5000000 INFO @ Mon, 29 Jun 2020 23:05:33: 1000000 INFO @ Mon, 29 Jun 2020 23:05:33: 6000000 INFO @ Mon, 29 Jun 2020 23:05:39: 2000000 INFO @ Mon, 29 Jun 2020 23:05:40: 7000000 INFO @ Mon, 29 Jun 2020 23:05:46: 3000000 INFO @ Mon, 29 Jun 2020 23:05:47: 8000000 INFO @ Mon, 29 Jun 2020 23:05:53: 4000000 INFO @ Mon, 29 Jun 2020 23:05:53: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:05:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:05:56: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:05:56: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:05:59: 5000000 INFO @ Mon, 29 Jun 2020 23:06:00: 10000000 INFO @ Mon, 29 Jun 2020 23:06:04: 1000000 INFO @ Mon, 29 Jun 2020 23:06:06: 6000000 INFO @ Mon, 29 Jun 2020 23:06:08: 11000000 INFO @ Mon, 29 Jun 2020 23:06:11: 2000000 INFO @ Mon, 29 Jun 2020 23:06:14: 7000000 INFO @ Mon, 29 Jun 2020 23:06:15: 12000000 INFO @ Mon, 29 Jun 2020 23:06:19: 3000000 INFO @ Mon, 29 Jun 2020 23:06:21: 8000000 INFO @ Mon, 29 Jun 2020 23:06:23: 13000000 INFO @ Mon, 29 Jun 2020 23:06:26: 4000000 INFO @ Mon, 29 Jun 2020 23:06:29: 9000000 INFO @ Mon, 29 Jun 2020 23:06:30: 14000000 INFO @ Mon, 29 Jun 2020 23:06:34: 5000000 INFO @ Mon, 29 Jun 2020 23:06:36: 10000000 INFO @ Mon, 29 Jun 2020 23:06:37: 15000000 INFO @ Mon, 29 Jun 2020 23:06:39: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:06:39: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:06:39: #1 total tags in treatment: 15165446 INFO @ Mon, 29 Jun 2020 23:06:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:06:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:06:39: #1 tags after filtering in treatment: 15165446 INFO @ Mon, 29 Jun 2020 23:06:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:06:39: #1 finished! INFO @ Mon, 29 Jun 2020 23:06:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:06:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:06:40: #2 number of paired peaks: 90 WARNING @ Mon, 29 Jun 2020 23:06:40: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:06:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:06:42: 6000000 INFO @ Mon, 29 Jun 2020 23:06:43: 11000000 INFO @ Mon, 29 Jun 2020 23:06:49: 7000000 INFO @ Mon, 29 Jun 2020 23:06:51: 12000000 INFO @ Mon, 29 Jun 2020 23:06:57: 8000000 INFO @ Mon, 29 Jun 2020 23:06:58: 13000000 INFO @ Mon, 29 Jun 2020 23:07:05: 9000000 INFO @ Mon, 29 Jun 2020 23:07:05: 14000000 INFO @ Mon, 29 Jun 2020 23:07:12: 15000000 INFO @ Mon, 29 Jun 2020 23:07:13: 10000000 INFO @ Mon, 29 Jun 2020 23:07:13: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:07:13: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:07:13: #1 total tags in treatment: 15165446 INFO @ Mon, 29 Jun 2020 23:07:13: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:07:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:07:14: #1 tags after filtering in treatment: 15165446 INFO @ Mon, 29 Jun 2020 23:07:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:07:14: #1 finished! INFO @ Mon, 29 Jun 2020 23:07:14: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:07:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:07:15: #2 number of paired peaks: 90 WARNING @ Mon, 29 Jun 2020 23:07:15: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:07:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:07:20: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:07:28: 12000000 INFO @ Mon, 29 Jun 2020 23:07:35: 13000000 INFO @ Mon, 29 Jun 2020 23:07:42: 14000000 INFO @ Mon, 29 Jun 2020 23:07:49: 15000000 INFO @ Mon, 29 Jun 2020 23:07:50: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:07:50: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:07:50: #1 total tags in treatment: 15165446 INFO @ Mon, 29 Jun 2020 23:07:50: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:07:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:07:50: #1 tags after filtering in treatment: 15165446 INFO @ Mon, 29 Jun 2020 23:07:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:07:50: #1 finished! INFO @ Mon, 29 Jun 2020 23:07:50: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:07:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:07:51: #2 number of paired peaks: 90 WARNING @ Mon, 29 Jun 2020 23:07:51: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:07:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287717/SRX287717.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。