Job ID = 6527812 SRX = SRX287714 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:58:34 prefetch.2.10.7: 1) Downloading 'SRR869902'... 2020-06-29T13:58:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:02:00 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:02:00 prefetch.2.10.7: 1) 'SRR869902' was downloaded successfully Read 21014950 spots for SRR869902/SRR869902.sra Written 21014950 spots for SRR869902/SRR869902.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:12 21014950 reads; of these: 21014950 (100.00%) were unpaired; of these: 1185137 (5.64%) aligned 0 times 14968794 (71.23%) aligned exactly 1 time 4861019 (23.13%) aligned >1 times 94.36% overall alignment rate Time searching: 00:07:12 Overall time: 00:07:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2682468 / 19829813 = 0.1353 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:19:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:19:53: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:19:53: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:20:00: 1000000 INFO @ Mon, 29 Jun 2020 23:20:07: 2000000 INFO @ Mon, 29 Jun 2020 23:20:14: 3000000 INFO @ Mon, 29 Jun 2020 23:20:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:20:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:20:23: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:20:23: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:20:27: 5000000 INFO @ Mon, 29 Jun 2020 23:20:30: 1000000 INFO @ Mon, 29 Jun 2020 23:20:35: 6000000 INFO @ Mon, 29 Jun 2020 23:20:37: 2000000 INFO @ Mon, 29 Jun 2020 23:20:41: 7000000 INFO @ Mon, 29 Jun 2020 23:20:44: 3000000 INFO @ Mon, 29 Jun 2020 23:20:48: 8000000 INFO @ Mon, 29 Jun 2020 23:20:51: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:20:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:20:53: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:20:53: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:20:54: 9000000 INFO @ Mon, 29 Jun 2020 23:20:59: 5000000 INFO @ Mon, 29 Jun 2020 23:21:00: 1000000 INFO @ Mon, 29 Jun 2020 23:21:01: 10000000 INFO @ Mon, 29 Jun 2020 23:21:06: 6000000 INFO @ Mon, 29 Jun 2020 23:21:06: 2000000 INFO @ Mon, 29 Jun 2020 23:21:08: 11000000 INFO @ Mon, 29 Jun 2020 23:21:13: 3000000 INFO @ Mon, 29 Jun 2020 23:21:13: 7000000 INFO @ Mon, 29 Jun 2020 23:21:15: 12000000 INFO @ Mon, 29 Jun 2020 23:21:19: 4000000 INFO @ Mon, 29 Jun 2020 23:21:20: 8000000 INFO @ Mon, 29 Jun 2020 23:21:23: 13000000 INFO @ Mon, 29 Jun 2020 23:21:26: 5000000 INFO @ Mon, 29 Jun 2020 23:21:28: 9000000 INFO @ Mon, 29 Jun 2020 23:21:30: 14000000 INFO @ Mon, 29 Jun 2020 23:21:32: 6000000 INFO @ Mon, 29 Jun 2020 23:21:35: 10000000 INFO @ Mon, 29 Jun 2020 23:21:36: 15000000 INFO @ Mon, 29 Jun 2020 23:21:39: 7000000 INFO @ Mon, 29 Jun 2020 23:21:42: 11000000 INFO @ Mon, 29 Jun 2020 23:21:43: 16000000 INFO @ Mon, 29 Jun 2020 23:21:45: 8000000 INFO @ Mon, 29 Jun 2020 23:21:48: 12000000 INFO @ Mon, 29 Jun 2020 23:21:49: 17000000 INFO @ Mon, 29 Jun 2020 23:21:50: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:21:50: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:21:50: #1 total tags in treatment: 17147345 INFO @ Mon, 29 Jun 2020 23:21:50: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:21:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:21:51: #1 tags after filtering in treatment: 17147345 INFO @ Mon, 29 Jun 2020 23:21:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:21:51: #1 finished! INFO @ Mon, 29 Jun 2020 23:21:51: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:21:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:21:52: 9000000 INFO @ Mon, 29 Jun 2020 23:21:52: #2 number of paired peaks: 66 WARNING @ Mon, 29 Jun 2020 23:21:52: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:21:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:21:56: 13000000 INFO @ Mon, 29 Jun 2020 23:21:58: 10000000 INFO @ Mon, 29 Jun 2020 23:22:03: 14000000 INFO @ Mon, 29 Jun 2020 23:22:04: 11000000 INFO @ Mon, 29 Jun 2020 23:22:10: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:22:11: 12000000 INFO @ Mon, 29 Jun 2020 23:22:17: 16000000 INFO @ Mon, 29 Jun 2020 23:22:17: 13000000 INFO @ Mon, 29 Jun 2020 23:22:24: 17000000 INFO @ Mon, 29 Jun 2020 23:22:24: 14000000 INFO @ Mon, 29 Jun 2020 23:22:25: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:22:25: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:22:25: #1 total tags in treatment: 17147345 INFO @ Mon, 29 Jun 2020 23:22:25: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:22:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:22:25: #1 tags after filtering in treatment: 17147345 INFO @ Mon, 29 Jun 2020 23:22:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:22:25: #1 finished! INFO @ Mon, 29 Jun 2020 23:22:25: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:22:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:22:26: #2 number of paired peaks: 66 WARNING @ Mon, 29 Jun 2020 23:22:26: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:22:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:22:30: 15000000 INFO @ Mon, 29 Jun 2020 23:22:36: 16000000 INFO @ Mon, 29 Jun 2020 23:22:42: 17000000 INFO @ Mon, 29 Jun 2020 23:22:43: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:22:43: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:22:43: #1 total tags in treatment: 17147345 INFO @ Mon, 29 Jun 2020 23:22:43: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:22:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:22:43: #1 tags after filtering in treatment: 17147345 INFO @ Mon, 29 Jun 2020 23:22:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:22:43: #1 finished! INFO @ Mon, 29 Jun 2020 23:22:43: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:22:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:22:44: #2 number of paired peaks: 66 WARNING @ Mon, 29 Jun 2020 23:22:44: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:22:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287714/SRX287714.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。