Job ID = 1294494 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-06-02T23:25:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:29:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:29:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:29:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:29:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:30:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:32:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:32:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 22,109,731 reads read : 22,109,731 reads written : 22,109,731 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:46 22109731 reads; of these: 22109731 (100.00%) were unpaired; of these: 1293802 (5.85%) aligned 0 times 13813370 (62.48%) aligned exactly 1 time 7002559 (31.67%) aligned >1 times 94.15% overall alignment rate Time searching: 00:09:47 Overall time: 00:09:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3522125 / 20815929 = 0.1692 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 08:52:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 08:52:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 08:52:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 08:52:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 08:52:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 08:52:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 08:52:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 08:52:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 08:52:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 08:52:57: 1000000 INFO @ Mon, 03 Jun 2019 08:52:57: 1000000 INFO @ Mon, 03 Jun 2019 08:52:58: 1000000 INFO @ Mon, 03 Jun 2019 08:53:06: 2000000 INFO @ Mon, 03 Jun 2019 08:53:06: 2000000 INFO @ Mon, 03 Jun 2019 08:53:07: 2000000 INFO @ Mon, 03 Jun 2019 08:53:15: 3000000 INFO @ Mon, 03 Jun 2019 08:53:16: 3000000 INFO @ Mon, 03 Jun 2019 08:53:16: 3000000 INFO @ Mon, 03 Jun 2019 08:53:24: 4000000 INFO @ Mon, 03 Jun 2019 08:53:25: 4000000 INFO @ Mon, 03 Jun 2019 08:53:25: 4000000 INFO @ Mon, 03 Jun 2019 08:53:33: 5000000 INFO @ Mon, 03 Jun 2019 08:53:34: 5000000 INFO @ Mon, 03 Jun 2019 08:53:34: 5000000 INFO @ Mon, 03 Jun 2019 08:53:42: 6000000 INFO @ Mon, 03 Jun 2019 08:53:43: 6000000 INFO @ Mon, 03 Jun 2019 08:53:44: 6000000 INFO @ Mon, 03 Jun 2019 08:53:51: 7000000 INFO @ Mon, 03 Jun 2019 08:53:52: 7000000 INFO @ Mon, 03 Jun 2019 08:53:53: 7000000 INFO @ Mon, 03 Jun 2019 08:54:00: 8000000 INFO @ Mon, 03 Jun 2019 08:54:01: 8000000 INFO @ Mon, 03 Jun 2019 08:54:02: 8000000 INFO @ Mon, 03 Jun 2019 08:54:09: 9000000 INFO @ Mon, 03 Jun 2019 08:54:10: 9000000 INFO @ Mon, 03 Jun 2019 08:54:11: 9000000 INFO @ Mon, 03 Jun 2019 08:54:17: 10000000 INFO @ Mon, 03 Jun 2019 08:54:19: 10000000 INFO @ Mon, 03 Jun 2019 08:54:20: 10000000 INFO @ Mon, 03 Jun 2019 08:54:26: 11000000 INFO @ Mon, 03 Jun 2019 08:54:28: 11000000 INFO @ Mon, 03 Jun 2019 08:54:29: 11000000 INFO @ Mon, 03 Jun 2019 08:54:35: 12000000 INFO @ Mon, 03 Jun 2019 08:54:37: 12000000 INFO @ Mon, 03 Jun 2019 08:54:38: 12000000 INFO @ Mon, 03 Jun 2019 08:54:44: 13000000 INFO @ Mon, 03 Jun 2019 08:54:46: 13000000 INFO @ Mon, 03 Jun 2019 08:54:47: 13000000 INFO @ Mon, 03 Jun 2019 08:54:53: 14000000 INFO @ Mon, 03 Jun 2019 08:54:55: 14000000 INFO @ Mon, 03 Jun 2019 08:54:56: 14000000 INFO @ Mon, 03 Jun 2019 08:55:02: 15000000 INFO @ Mon, 03 Jun 2019 08:55:04: 15000000 INFO @ Mon, 03 Jun 2019 08:55:05: 15000000 INFO @ Mon, 03 Jun 2019 08:55:11: 16000000 INFO @ Mon, 03 Jun 2019 08:55:13: 16000000 INFO @ Mon, 03 Jun 2019 08:55:13: 16000000 INFO @ Mon, 03 Jun 2019 08:55:20: 17000000 INFO @ Mon, 03 Jun 2019 08:55:22: 17000000 INFO @ Mon, 03 Jun 2019 08:55:22: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 08:55:22: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 08:55:22: #1 total tags in treatment: 17293804 INFO @ Mon, 03 Jun 2019 08:55:22: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 08:55:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 08:55:22: 17000000 INFO @ Mon, 03 Jun 2019 08:55:23: #1 tags after filtering in treatment: 17293804 INFO @ Mon, 03 Jun 2019 08:55:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 08:55:23: #1 finished! INFO @ Mon, 03 Jun 2019 08:55:23: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 08:55:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 08:55:24: #2 number of paired peaks: 194 WARNING @ Mon, 03 Jun 2019 08:55:24: Fewer paired peaks (194) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 194 pairs to build model! INFO @ Mon, 03 Jun 2019 08:55:24: start model_add_line... INFO @ Mon, 03 Jun 2019 08:55:24: start X-correlation... INFO @ Mon, 03 Jun 2019 08:55:24: end of X-cor INFO @ Mon, 03 Jun 2019 08:55:24: #2 finished! INFO @ Mon, 03 Jun 2019 08:55:24: #2 predicted fragment length is 47 bps INFO @ Mon, 03 Jun 2019 08:55:24: #2 alternative fragment length(s) may be 47 bps INFO @ Mon, 03 Jun 2019 08:55:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.05_model.r WARNING @ Mon, 03 Jun 2019 08:55:24: #2 Since the d (47) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 08:55:24: #2 You may need to consider one of the other alternative d(s): 47 WARNING @ Mon, 03 Jun 2019 08:55:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 08:55:24: #3 Call peaks... INFO @ Mon, 03 Jun 2019 08:55:24: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 08:55:25: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 08:55:25: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 08:55:25: #1 total tags in treatment: 17293804 INFO @ Mon, 03 Jun 2019 08:55:25: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 08:55:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 08:55:25: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 08:55:25: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 08:55:25: #1 total tags in treatment: 17293804 INFO @ Mon, 03 Jun 2019 08:55:25: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 08:55:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 08:55:25: #1 tags after filtering in treatment: 17293804 INFO @ Mon, 03 Jun 2019 08:55:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 08:55:25: #1 finished! INFO @ Mon, 03 Jun 2019 08:55:25: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 08:55:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 08:55:25: #1 tags after filtering in treatment: 17293804 INFO @ Mon, 03 Jun 2019 08:55:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 08:55:25: #1 finished! INFO @ Mon, 03 Jun 2019 08:55:25: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 08:55:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 08:55:27: #2 number of paired peaks: 194 WARNING @ Mon, 03 Jun 2019 08:55:27: Fewer paired peaks (194) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 194 pairs to build model! INFO @ Mon, 03 Jun 2019 08:55:27: start model_add_line... INFO @ Mon, 03 Jun 2019 08:55:27: start X-correlation... INFO @ Mon, 03 Jun 2019 08:55:27: end of X-cor INFO @ Mon, 03 Jun 2019 08:55:27: #2 finished! INFO @ Mon, 03 Jun 2019 08:55:27: #2 predicted fragment length is 47 bps INFO @ Mon, 03 Jun 2019 08:55:27: #2 alternative fragment length(s) may be 47 bps INFO @ Mon, 03 Jun 2019 08:55:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.20_model.r WARNING @ Mon, 03 Jun 2019 08:55:27: #2 Since the d (47) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 08:55:27: #2 You may need to consider one of the other alternative d(s): 47 WARNING @ Mon, 03 Jun 2019 08:55:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 08:55:27: #3 Call peaks... INFO @ Mon, 03 Jun 2019 08:55:27: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 08:55:27: #2 number of paired peaks: 194 WARNING @ Mon, 03 Jun 2019 08:55:27: Fewer paired peaks (194) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 194 pairs to build model! INFO @ Mon, 03 Jun 2019 08:55:27: start model_add_line... INFO @ Mon, 03 Jun 2019 08:55:27: start X-correlation... INFO @ Mon, 03 Jun 2019 08:55:27: end of X-cor INFO @ Mon, 03 Jun 2019 08:55:27: #2 finished! INFO @ Mon, 03 Jun 2019 08:55:27: #2 predicted fragment length is 47 bps INFO @ Mon, 03 Jun 2019 08:55:27: #2 alternative fragment length(s) may be 47 bps INFO @ Mon, 03 Jun 2019 08:55:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.10_model.r WARNING @ Mon, 03 Jun 2019 08:55:27: #2 Since the d (47) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 08:55:27: #2 You may need to consider one of the other alternative d(s): 47 WARNING @ Mon, 03 Jun 2019 08:55:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 08:55:27: #3 Call peaks... INFO @ Mon, 03 Jun 2019 08:55:27: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 08:56:09: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 08:56:11: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 08:56:12: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 08:56:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.05_peaks.xls INFO @ Mon, 03 Jun 2019 08:56:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 08:56:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.05_summits.bed INFO @ Mon, 03 Jun 2019 08:56:31: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (2225 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 08:56:33: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.20_peaks.xls INFO @ Mon, 03 Jun 2019 08:56:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 08:56:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.20_summits.bed INFO @ Mon, 03 Jun 2019 08:56:33: Done! pass1 - making usageList (12 chroms): 2 millis pass2 - checking and writing primary data (1359 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 08:56:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.10_peaks.xls INFO @ Mon, 03 Jun 2019 08:56:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 08:56:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX287694/SRX287694.10_summits.bed INFO @ Mon, 03 Jun 2019 08:56:34: Done! pass1 - making usageList (13 chroms): 2 millis pass2 - checking and writing primary data (1819 records, 4 fields): 7 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。