Job ID = 6527803 SRX = SRX287681 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:43:59 prefetch.2.10.7: 1) Downloading 'SRR869869'... 2020-06-29T13:43:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:47:47 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:47:47 prefetch.2.10.7: 1) 'SRR869869' was downloaded successfully Read 19200904 spots for SRR869869/SRR869869.sra Written 19200904 spots for SRR869869/SRR869869.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:58 19200904 reads; of these: 19200904 (100.00%) were unpaired; of these: 1155378 (6.02%) aligned 0 times 13622333 (70.95%) aligned exactly 1 time 4423193 (23.04%) aligned >1 times 93.98% overall alignment rate Time searching: 00:06:58 Overall time: 00:06:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1406295 / 18045526 = 0.0779 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:05:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:05:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:05:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:05:41: 1000000 INFO @ Mon, 29 Jun 2020 23:05:47: 2000000 INFO @ Mon, 29 Jun 2020 23:05:53: 3000000 INFO @ Mon, 29 Jun 2020 23:05:59: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:04: 5000000 INFO @ Mon, 29 Jun 2020 23:06:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:05: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:05: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:11: 6000000 INFO @ Mon, 29 Jun 2020 23:06:13: 1000000 INFO @ Mon, 29 Jun 2020 23:06:18: 7000000 INFO @ Mon, 29 Jun 2020 23:06:21: 2000000 INFO @ Mon, 29 Jun 2020 23:06:25: 8000000 INFO @ Mon, 29 Jun 2020 23:06:29: 3000000 INFO @ Mon, 29 Jun 2020 23:06:31: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:36: 4000000 INFO @ Mon, 29 Jun 2020 23:06:38: 10000000 INFO @ Mon, 29 Jun 2020 23:06:42: 1000000 INFO @ Mon, 29 Jun 2020 23:06:44: 5000000 INFO @ Mon, 29 Jun 2020 23:06:45: 11000000 INFO @ Mon, 29 Jun 2020 23:06:49: 2000000 INFO @ Mon, 29 Jun 2020 23:06:52: 12000000 INFO @ Mon, 29 Jun 2020 23:06:52: 6000000 INFO @ Mon, 29 Jun 2020 23:06:56: 3000000 INFO @ Mon, 29 Jun 2020 23:06:59: 13000000 INFO @ Mon, 29 Jun 2020 23:07:00: 7000000 INFO @ Mon, 29 Jun 2020 23:07:03: 4000000 INFO @ Mon, 29 Jun 2020 23:07:06: 14000000 INFO @ Mon, 29 Jun 2020 23:07:08: 8000000 INFO @ Mon, 29 Jun 2020 23:07:10: 5000000 INFO @ Mon, 29 Jun 2020 23:07:13: 15000000 INFO @ Mon, 29 Jun 2020 23:07:16: 9000000 INFO @ Mon, 29 Jun 2020 23:07:17: 6000000 INFO @ Mon, 29 Jun 2020 23:07:20: 16000000 INFO @ Mon, 29 Jun 2020 23:07:24: 10000000 INFO @ Mon, 29 Jun 2020 23:07:24: 7000000 INFO @ Mon, 29 Jun 2020 23:07:26: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:07:26: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:07:26: #1 total tags in treatment: 16639231 INFO @ Mon, 29 Jun 2020 23:07:26: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:07:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:07:26: #1 tags after filtering in treatment: 16639231 INFO @ Mon, 29 Jun 2020 23:07:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:07:26: #1 finished! INFO @ Mon, 29 Jun 2020 23:07:26: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:07:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:07:27: #2 number of paired peaks: 54 WARNING @ Mon, 29 Jun 2020 23:07:27: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:07:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:07:31: 8000000 INFO @ Mon, 29 Jun 2020 23:07:32: 11000000 INFO @ Mon, 29 Jun 2020 23:07:38: 9000000 INFO @ Mon, 29 Jun 2020 23:07:40: 12000000 INFO @ Mon, 29 Jun 2020 23:07:45: 10000000 INFO @ Mon, 29 Jun 2020 23:07:48: 13000000 INFO @ Mon, 29 Jun 2020 23:07:52: 11000000 INFO @ Mon, 29 Jun 2020 23:07:56: 14000000 INFO @ Mon, 29 Jun 2020 23:07:59: 12000000 INFO @ Mon, 29 Jun 2020 23:08:03: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:08:07: 13000000 INFO @ Mon, 29 Jun 2020 23:08:11: 16000000 INFO @ Mon, 29 Jun 2020 23:08:14: 14000000 INFO @ Mon, 29 Jun 2020 23:08:16: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:08:16: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:08:16: #1 total tags in treatment: 16639231 INFO @ Mon, 29 Jun 2020 23:08:16: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:08:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:08:17: #1 tags after filtering in treatment: 16639231 INFO @ Mon, 29 Jun 2020 23:08:17: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:08:17: #1 finished! INFO @ Mon, 29 Jun 2020 23:08:17: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:08:17: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:08:18: #2 number of paired peaks: 54 WARNING @ Mon, 29 Jun 2020 23:08:18: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:08:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:08:21: 15000000 INFO @ Mon, 29 Jun 2020 23:08:27: 16000000 INFO @ Mon, 29 Jun 2020 23:08:31: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:08:31: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:08:31: #1 total tags in treatment: 16639231 INFO @ Mon, 29 Jun 2020 23:08:31: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:08:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:08:31: #1 tags after filtering in treatment: 16639231 INFO @ Mon, 29 Jun 2020 23:08:31: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:08:31: #1 finished! INFO @ Mon, 29 Jun 2020 23:08:31: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:08:31: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:08:32: #2 number of paired peaks: 54 WARNING @ Mon, 29 Jun 2020 23:08:32: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:08:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287681/SRX287681.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。