Job ID = 6527799 SRX = SRX287657 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:43:25 prefetch.2.10.7: 1) Downloading 'SRR869845'... 2020-06-29T13:43:25 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:47:35 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:47:35 prefetch.2.10.7: 1) 'SRR869845' was downloaded successfully Read 17419333 spots for SRR869845/SRR869845.sra Written 17419333 spots for SRR869845/SRR869845.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:55 17419333 reads; of these: 17419333 (100.00%) were unpaired; of these: 1038328 (5.96%) aligned 0 times 13169694 (75.60%) aligned exactly 1 time 3211311 (18.44%) aligned >1 times 94.04% overall alignment rate Time searching: 00:04:55 Overall time: 00:04:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1215060 / 16381005 = 0.0742 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:01:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:01:37: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:01:37: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:01:43: 1000000 INFO @ Mon, 29 Jun 2020 23:01:50: 2000000 INFO @ Mon, 29 Jun 2020 23:01:56: 3000000 INFO @ Mon, 29 Jun 2020 23:02:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:02:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:02:07: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:02:07: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:02:09: 5000000 INFO @ Mon, 29 Jun 2020 23:02:14: 1000000 INFO @ Mon, 29 Jun 2020 23:02:16: 6000000 INFO @ Mon, 29 Jun 2020 23:02:21: 2000000 INFO @ Mon, 29 Jun 2020 23:02:23: 7000000 INFO @ Mon, 29 Jun 2020 23:02:28: 3000000 INFO @ Mon, 29 Jun 2020 23:02:29: 8000000 INFO @ Mon, 29 Jun 2020 23:02:35: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:02:36: 9000000 INFO @ Mon, 29 Jun 2020 23:02:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:02:37: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:02:37: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:02:42: 5000000 INFO @ Mon, 29 Jun 2020 23:02:43: 10000000 INFO @ Mon, 29 Jun 2020 23:02:44: 1000000 INFO @ Mon, 29 Jun 2020 23:02:49: 6000000 INFO @ Mon, 29 Jun 2020 23:02:50: 11000000 INFO @ Mon, 29 Jun 2020 23:02:51: 2000000 INFO @ Mon, 29 Jun 2020 23:02:56: 7000000 INFO @ Mon, 29 Jun 2020 23:02:57: 12000000 INFO @ Mon, 29 Jun 2020 23:02:58: 3000000 INFO @ Mon, 29 Jun 2020 23:03:03: 8000000 INFO @ Mon, 29 Jun 2020 23:03:04: 13000000 INFO @ Mon, 29 Jun 2020 23:03:05: 4000000 INFO @ Mon, 29 Jun 2020 23:03:10: 9000000 INFO @ Mon, 29 Jun 2020 23:03:11: 14000000 INFO @ Mon, 29 Jun 2020 23:03:12: 5000000 INFO @ Mon, 29 Jun 2020 23:03:17: 10000000 INFO @ Mon, 29 Jun 2020 23:03:18: 15000000 INFO @ Mon, 29 Jun 2020 23:03:19: 6000000 INFO @ Mon, 29 Jun 2020 23:03:19: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:03:19: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:03:19: #1 total tags in treatment: 15165945 INFO @ Mon, 29 Jun 2020 23:03:19: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:03:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:03:20: #1 tags after filtering in treatment: 15165945 INFO @ Mon, 29 Jun 2020 23:03:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:03:20: #1 finished! INFO @ Mon, 29 Jun 2020 23:03:20: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:03:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:03:21: #2 number of paired peaks: 84 WARNING @ Mon, 29 Jun 2020 23:03:21: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:03:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:03:24: 11000000 INFO @ Mon, 29 Jun 2020 23:03:26: 7000000 INFO @ Mon, 29 Jun 2020 23:03:31: 12000000 INFO @ Mon, 29 Jun 2020 23:03:33: 8000000 INFO @ Mon, 29 Jun 2020 23:03:38: 13000000 INFO @ Mon, 29 Jun 2020 23:03:40: 9000000 INFO @ Mon, 29 Jun 2020 23:03:45: 14000000 INFO @ Mon, 29 Jun 2020 23:03:47: 10000000 INFO @ Mon, 29 Jun 2020 23:03:52: 15000000 INFO @ Mon, 29 Jun 2020 23:03:53: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:03:53: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:03:53: #1 total tags in treatment: 15165945 INFO @ Mon, 29 Jun 2020 23:03:53: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:03:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:03:53: #1 tags after filtering in treatment: 15165945 INFO @ Mon, 29 Jun 2020 23:03:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:03:53: #1 finished! INFO @ Mon, 29 Jun 2020 23:03:53: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:03:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:03:54: 11000000 INFO @ Mon, 29 Jun 2020 23:03:54: #2 number of paired peaks: 84 WARNING @ Mon, 29 Jun 2020 23:03:54: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:03:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:04:01: 12000000 INFO @ Mon, 29 Jun 2020 23:04:07: 13000000 INFO @ Mon, 29 Jun 2020 23:04:14: 14000000 INFO @ Mon, 29 Jun 2020 23:04:20: 15000000 INFO @ Mon, 29 Jun 2020 23:04:22: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:04:22: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:04:22: #1 total tags in treatment: 15165945 INFO @ Mon, 29 Jun 2020 23:04:22: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:04:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:04:22: #1 tags after filtering in treatment: 15165945 INFO @ Mon, 29 Jun 2020 23:04:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:04:22: #1 finished! INFO @ Mon, 29 Jun 2020 23:04:22: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:04:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:04:23: #2 number of paired peaks: 84 WARNING @ Mon, 29 Jun 2020 23:04:23: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:04:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287657/SRX287657.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。