Job ID = 6527769 SRX = SRX2734370 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:19:35 prefetch.2.10.7: 1) Downloading 'SRR5445347'... 2020-06-29T13:19:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:22:11 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:22:11 prefetch.2.10.7: 1) 'SRR5445347' was downloaded successfully 2020-06-29T13:22:11 prefetch.2.10.7: 'SRR5445347' has 0 unresolved dependencies Read 32127329 spots for SRR5445347/SRR5445347.sra Written 32127329 spots for SRR5445347/SRR5445347.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:10 32127329 reads; of these: 32127329 (100.00%) were unpaired; of these: 1648702 (5.13%) aligned 0 times 25908183 (80.64%) aligned exactly 1 time 4570444 (14.23%) aligned >1 times 94.87% overall alignment rate Time searching: 00:07:10 Overall time: 00:07:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3591209 / 30478627 = 0.1178 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:47:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:47:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:47:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:47:41: 1000000 INFO @ Mon, 29 Jun 2020 22:47:46: 2000000 INFO @ Mon, 29 Jun 2020 22:47:52: 3000000 INFO @ Mon, 29 Jun 2020 22:47:57: 4000000 INFO @ Mon, 29 Jun 2020 22:48:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:48:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:48:05: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:48:05: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:48:08: 6000000 INFO @ Mon, 29 Jun 2020 22:48:11: 1000000 INFO @ Mon, 29 Jun 2020 22:48:14: 7000000 INFO @ Mon, 29 Jun 2020 22:48:17: 2000000 INFO @ Mon, 29 Jun 2020 22:48:19: 8000000 INFO @ Mon, 29 Jun 2020 22:48:22: 3000000 INFO @ Mon, 29 Jun 2020 22:48:25: 9000000 INFO @ Mon, 29 Jun 2020 22:48:27: 4000000 INFO @ Mon, 29 Jun 2020 22:48:30: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:48:33: 5000000 INFO @ Mon, 29 Jun 2020 22:48:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:48:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:48:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:48:36: 11000000 INFO @ Mon, 29 Jun 2020 22:48:39: 6000000 INFO @ Mon, 29 Jun 2020 22:48:40: 1000000 INFO @ Mon, 29 Jun 2020 22:48:41: 12000000 INFO @ Mon, 29 Jun 2020 22:48:45: 7000000 INFO @ Mon, 29 Jun 2020 22:48:45: 2000000 INFO @ Mon, 29 Jun 2020 22:48:47: 13000000 INFO @ Mon, 29 Jun 2020 22:48:50: 3000000 INFO @ Mon, 29 Jun 2020 22:48:50: 8000000 INFO @ Mon, 29 Jun 2020 22:48:53: 14000000 INFO @ Mon, 29 Jun 2020 22:48:55: 4000000 INFO @ Mon, 29 Jun 2020 22:48:56: 9000000 INFO @ Mon, 29 Jun 2020 22:48:58: 15000000 INFO @ Mon, 29 Jun 2020 22:49:00: 5000000 INFO @ Mon, 29 Jun 2020 22:49:01: 10000000 INFO @ Mon, 29 Jun 2020 22:49:04: 16000000 INFO @ Mon, 29 Jun 2020 22:49:05: 6000000 INFO @ Mon, 29 Jun 2020 22:49:07: 11000000 INFO @ Mon, 29 Jun 2020 22:49:09: 17000000 INFO @ Mon, 29 Jun 2020 22:49:11: 7000000 INFO @ Mon, 29 Jun 2020 22:49:12: 12000000 INFO @ Mon, 29 Jun 2020 22:49:15: 18000000 INFO @ Mon, 29 Jun 2020 22:49:16: 8000000 INFO @ Mon, 29 Jun 2020 22:49:18: 13000000 INFO @ Mon, 29 Jun 2020 22:49:20: 19000000 INFO @ Mon, 29 Jun 2020 22:49:21: 9000000 INFO @ Mon, 29 Jun 2020 22:49:24: 14000000 INFO @ Mon, 29 Jun 2020 22:49:26: 10000000 INFO @ Mon, 29 Jun 2020 22:49:26: 20000000 INFO @ Mon, 29 Jun 2020 22:49:29: 15000000 INFO @ Mon, 29 Jun 2020 22:49:31: 11000000 INFO @ Mon, 29 Jun 2020 22:49:32: 21000000 INFO @ Mon, 29 Jun 2020 22:49:35: 16000000 INFO @ Mon, 29 Jun 2020 22:49:36: 12000000 INFO @ Mon, 29 Jun 2020 22:49:37: 22000000 INFO @ Mon, 29 Jun 2020 22:49:41: 13000000 INFO @ Mon, 29 Jun 2020 22:49:41: 17000000 INFO @ Mon, 29 Jun 2020 22:49:43: 23000000 INFO @ Mon, 29 Jun 2020 22:49:46: 14000000 INFO @ Mon, 29 Jun 2020 22:49:47: 18000000 INFO @ Mon, 29 Jun 2020 22:49:49: 24000000 INFO @ Mon, 29 Jun 2020 22:49:51: 15000000 INFO @ Mon, 29 Jun 2020 22:49:52: 19000000 INFO @ Mon, 29 Jun 2020 22:49:54: 25000000 INFO @ Mon, 29 Jun 2020 22:49:56: 16000000 INFO @ Mon, 29 Jun 2020 22:49:58: 20000000 INFO @ Mon, 29 Jun 2020 22:50:00: 26000000 INFO @ Mon, 29 Jun 2020 22:50:01: 17000000 INFO @ Mon, 29 Jun 2020 22:50:04: 21000000 INFO @ Mon, 29 Jun 2020 22:50:05: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:50:05: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:50:05: #1 total tags in treatment: 26887418 INFO @ Mon, 29 Jun 2020 22:50:05: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:50:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:50:06: #1 tags after filtering in treatment: 26887418 INFO @ Mon, 29 Jun 2020 22:50:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:50:06: #1 finished! INFO @ Mon, 29 Jun 2020 22:50:06: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:50:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:50:06: 18000000 INFO @ Mon, 29 Jun 2020 22:50:07: #2 number of paired peaks: 88 WARNING @ Mon, 29 Jun 2020 22:50:07: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:50:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:50:09: 22000000 INFO @ Mon, 29 Jun 2020 22:50:11: 19000000 INFO @ Mon, 29 Jun 2020 22:50:14: 23000000 INFO @ Mon, 29 Jun 2020 22:50:16: 20000000 INFO @ Mon, 29 Jun 2020 22:50:19: 24000000 INFO @ Mon, 29 Jun 2020 22:50:21: 21000000 INFO @ Mon, 29 Jun 2020 22:50:24: 25000000 INFO @ Mon, 29 Jun 2020 22:50:26: 22000000 INFO @ Mon, 29 Jun 2020 22:50:30: 26000000 INFO @ Mon, 29 Jun 2020 22:50:31: 23000000 INFO @ Mon, 29 Jun 2020 22:50:34: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:50:34: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:50:34: #1 total tags in treatment: 26887418 INFO @ Mon, 29 Jun 2020 22:50:34: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:50:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:50:35: #1 tags after filtering in treatment: 26887418 INFO @ Mon, 29 Jun 2020 22:50:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:50:35: #1 finished! INFO @ Mon, 29 Jun 2020 22:50:35: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:50:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:50:36: 24000000 INFO @ Mon, 29 Jun 2020 22:50:36: #2 number of paired peaks: 88 WARNING @ Mon, 29 Jun 2020 22:50:36: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:50:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:50:41: 25000000 INFO @ Mon, 29 Jun 2020 22:50:46: 26000000 INFO @ Mon, 29 Jun 2020 22:50:51: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:50:51: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:50:51: #1 total tags in treatment: 26887418 INFO @ Mon, 29 Jun 2020 22:50:51: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:50:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:50:51: #1 tags after filtering in treatment: 26887418 INFO @ Mon, 29 Jun 2020 22:50:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:50:51: #1 finished! INFO @ Mon, 29 Jun 2020 22:50:51: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:50:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:50:53: #2 number of paired peaks: 88 WARNING @ Mon, 29 Jun 2020 22:50:53: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:50:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2734370/SRX2734370.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。