Job ID = 9157943 sra ファイルのダウンロード中... Completed: 1613275K bytes transferred in 35 seconds (371702K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 44637373 spots for /home/okishinya/chipatlas/results/dm3/SRX2734361/SRR5445338.sra Written 44637373 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:43:29 44637373 reads; of these: 44637373 (100.00%) were unpaired; of these: 44587410 (99.89%) aligned 0 times 4456 (0.01%) aligned exactly 1 time 45507 (0.10%) aligned >1 times 0.11% overall alignment rate Time searching: 00:43:29 Overall time: 00:43:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 44905 / 49963 = 0.8988 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 14:58:24: # Command line: callpeak -t SRX2734361.bam -f BAM -g dm -n SRX2734361.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2734361.05 # format = BAM # ChIP-seq file = ['SRX2734361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 14:58:24: #1 read tag files... INFO @ Tue, 27 Jun 2017 14:58:24: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 14:58:24: # Command line: callpeak -t SRX2734361.bam -f BAM -g dm -n SRX2734361.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2734361.20 # format = BAM # ChIP-seq file = ['SRX2734361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 14:58:24: #1 read tag files... INFO @ Tue, 27 Jun 2017 14:58:24: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 14:58:24: # Command line: callpeak -t SRX2734361.bam -f BAM -g dm -n SRX2734361.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2734361.10 # format = BAM # ChIP-seq file = ['SRX2734361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 14:58:24: #1 read tag files... INFO @ Tue, 27 Jun 2017 14:58:24: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 14:58:24: #1 tag size is determined as 100 bps INFO @ Tue, 27 Jun 2017 14:58:24: #1 tag size = 100 INFO @ Tue, 27 Jun 2017 14:58:24: #1 total tags in treatment: 5058 INFO @ Tue, 27 Jun 2017 14:58:24: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 14:58:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 14:58:24: #1 tag size is determined as 100 bps INFO @ Tue, 27 Jun 2017 14:58:24: #1 tag size = 100 INFO @ Tue, 27 Jun 2017 14:58:24: #1 total tags in treatment: 5058 INFO @ Tue, 27 Jun 2017 14:58:24: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 14:58:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 14:58:24: #1 tag size is determined as 100 bps INFO @ Tue, 27 Jun 2017 14:58:24: #1 tag size = 100 INFO @ Tue, 27 Jun 2017 14:58:24: #1 total tags in treatment: 5058 INFO @ Tue, 27 Jun 2017 14:58:24: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 14:58:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 14:58:24: #1 tags after filtering in treatment: 5057 INFO @ Tue, 27 Jun 2017 14:58:24: #1 tags after filtering in treatment: 5057 INFO @ Tue, 27 Jun 2017 14:58:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 14:58:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 14:58:24: #1 finished! INFO @ Tue, 27 Jun 2017 14:58:24: #1 finished! INFO @ Tue, 27 Jun 2017 14:58:24: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 14:58:24: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 14:58:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 14:58:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 14:58:24: #1 tags after filtering in treatment: 5057 INFO @ Tue, 27 Jun 2017 14:58:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 14:58:24: #1 finished! INFO @ Tue, 27 Jun 2017 14:58:24: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 14:58:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 14:58:24: #2 number of paired peaks: 18 INFO @ Tue, 27 Jun 2017 14:58:24: #2 number of paired peaks: 18 WARNING @ Tue, 27 Jun 2017 14:58:24: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 14:58:24: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 14:58:24: Process for pairing-model is terminated! WARNING @ Tue, 27 Jun 2017 14:58:24: Process for pairing-model is terminated! INFO @ Tue, 27 Jun 2017 14:58:24: #2 number of paired peaks: 18 WARNING @ Tue, 27 Jun 2017 14:58:24: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 14:58:24: Process for pairing-model is terminated! cat: SRX2734361.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX2734361.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX2734361.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2734361.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2734361.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2734361.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis CompletedMACS2peakCalling rm: cannot remove `SRX2734361.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2734361.20_*.xls': そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2734361.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling rm: cannot remove `SRX2734361.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2734361.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2734361.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。