Job ID = 1294366 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-06-02T22:00:43 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:00:43 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:00:43 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:18:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:27:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:28:30 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:30:26 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:31:45 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:55:57 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:02:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:29:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:29:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:30:11 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:34:30 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:36:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:37:10 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T23:39:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 92,189,884 reads read : 184,379,768 reads written : 184,379,768 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 10:17:03 92189884 reads; of these: 92189884 (100.00%) were paired; of these: 12212887 (13.25%) aligned concordantly 0 times 25306216 (27.45%) aligned concordantly exactly 1 time 54670781 (59.30%) aligned concordantly >1 times ---- 12212887 pairs aligned concordantly 0 times; of these: 2107298 (17.25%) aligned discordantly 1 time ---- 10105589 pairs aligned 0 times concordantly or discordantly; of these: 20211178 mates make up the pairs; of these: 6617820 (32.74%) aligned 0 times 1846588 (9.14%) aligned exactly 1 time 11746770 (58.12%) aligned >1 times 96.41% overall alignment rate Time searching: 10:17:03 Overall time: 10:17:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 76 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 60701495 / 81961759 = 0.7406 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 20:22:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:22:24: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:22:24: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:22:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:22:24: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:22:24: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:22:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:22:24: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:22:24: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:22:32: 1000000 INFO @ Mon, 03 Jun 2019 20:22:32: 1000000 INFO @ Mon, 03 Jun 2019 20:22:34: 1000000 INFO @ Mon, 03 Jun 2019 20:22:40: 2000000 INFO @ Mon, 03 Jun 2019 20:22:40: 2000000 INFO @ Mon, 03 Jun 2019 20:22:43: 2000000 INFO @ Mon, 03 Jun 2019 20:22:47: 3000000 INFO @ Mon, 03 Jun 2019 20:22:48: 3000000 INFO @ Mon, 03 Jun 2019 20:22:52: 3000000 INFO @ Mon, 03 Jun 2019 20:22:54: 4000000 INFO @ Mon, 03 Jun 2019 20:22:55: 4000000 INFO @ Mon, 03 Jun 2019 20:23:00: 4000000 INFO @ Mon, 03 Jun 2019 20:23:01: 5000000 INFO @ Mon, 03 Jun 2019 20:23:02: 5000000 INFO @ Mon, 03 Jun 2019 20:23:08: 6000000 INFO @ Mon, 03 Jun 2019 20:23:09: 5000000 INFO @ Mon, 03 Jun 2019 20:23:10: 6000000 INFO @ Mon, 03 Jun 2019 20:23:15: 7000000 INFO @ Mon, 03 Jun 2019 20:23:17: 7000000 INFO @ Mon, 03 Jun 2019 20:23:18: 6000000 INFO @ Mon, 03 Jun 2019 20:23:22: 8000000 INFO @ Mon, 03 Jun 2019 20:23:24: 8000000 INFO @ Mon, 03 Jun 2019 20:23:27: 7000000 INFO @ Mon, 03 Jun 2019 20:23:29: 9000000 INFO @ Mon, 03 Jun 2019 20:23:31: 9000000 INFO @ Mon, 03 Jun 2019 20:23:35: 8000000 INFO @ Mon, 03 Jun 2019 20:23:36: 10000000 INFO @ Mon, 03 Jun 2019 20:23:39: 10000000 INFO @ Mon, 03 Jun 2019 20:23:44: 11000000 INFO @ Mon, 03 Jun 2019 20:23:44: 9000000 INFO @ Mon, 03 Jun 2019 20:23:46: 11000000 INFO @ Mon, 03 Jun 2019 20:23:51: 12000000 INFO @ Mon, 03 Jun 2019 20:23:52: 10000000 INFO @ Mon, 03 Jun 2019 20:23:53: 12000000 INFO @ Mon, 03 Jun 2019 20:23:57: 13000000 INFO @ Mon, 03 Jun 2019 20:24:00: 13000000 INFO @ Mon, 03 Jun 2019 20:24:01: 11000000 INFO @ Mon, 03 Jun 2019 20:24:04: 14000000 INFO @ Mon, 03 Jun 2019 20:24:07: 14000000 INFO @ Mon, 03 Jun 2019 20:24:09: 12000000 INFO @ Mon, 03 Jun 2019 20:24:12: 15000000 INFO @ Mon, 03 Jun 2019 20:24:15: 15000000 INFO @ Mon, 03 Jun 2019 20:24:18: 13000000 INFO @ Mon, 03 Jun 2019 20:24:19: 16000000 INFO @ Mon, 03 Jun 2019 20:24:23: 16000000 INFO @ Mon, 03 Jun 2019 20:24:26: 14000000 INFO @ Mon, 03 Jun 2019 20:24:27: 17000000 INFO @ Mon, 03 Jun 2019 20:24:30: 17000000 INFO @ Mon, 03 Jun 2019 20:24:34: 18000000 INFO @ Mon, 03 Jun 2019 20:24:35: 15000000 INFO @ Mon, 03 Jun 2019 20:24:38: 18000000 INFO @ Mon, 03 Jun 2019 20:24:41: 19000000 INFO @ Mon, 03 Jun 2019 20:24:45: 16000000 INFO @ Mon, 03 Jun 2019 20:24:46: 19000000 INFO @ Mon, 03 Jun 2019 20:24:48: 20000000 INFO @ Mon, 03 Jun 2019 20:24:53: 20000000 INFO @ Mon, 03 Jun 2019 20:24:54: 17000000 INFO @ Mon, 03 Jun 2019 20:24:56: 21000000 INFO @ Mon, 03 Jun 2019 20:25:01: 21000000 INFO @ Mon, 03 Jun 2019 20:25:03: 18000000 INFO @ Mon, 03 Jun 2019 20:25:03: 22000000 INFO @ Mon, 03 Jun 2019 20:25:09: 22000000 INFO @ Mon, 03 Jun 2019 20:25:11: 23000000 INFO @ Mon, 03 Jun 2019 20:25:12: 19000000 INFO @ Mon, 03 Jun 2019 20:25:16: 23000000 INFO @ Mon, 03 Jun 2019 20:25:18: 24000000 INFO @ Mon, 03 Jun 2019 20:25:22: 20000000 INFO @ Mon, 03 Jun 2019 20:25:24: 24000000 INFO @ Mon, 03 Jun 2019 20:25:26: 25000000 INFO @ Mon, 03 Jun 2019 20:25:31: 21000000 INFO @ Mon, 03 Jun 2019 20:25:32: 25000000 INFO @ Mon, 03 Jun 2019 20:25:33: 26000000 INFO @ Mon, 03 Jun 2019 20:25:40: 26000000 INFO @ Mon, 03 Jun 2019 20:25:40: 22000000 INFO @ Mon, 03 Jun 2019 20:25:41: 27000000 INFO @ Mon, 03 Jun 2019 20:25:48: 27000000 INFO @ Mon, 03 Jun 2019 20:25:48: 28000000 INFO @ Mon, 03 Jun 2019 20:25:51: 23000000 INFO @ Mon, 03 Jun 2019 20:25:55: 28000000 INFO @ Mon, 03 Jun 2019 20:25:56: 29000000 INFO @ Mon, 03 Jun 2019 20:26:01: 24000000 INFO @ Mon, 03 Jun 2019 20:26:03: 30000000 INFO @ Mon, 03 Jun 2019 20:26:03: 29000000 INFO @ Mon, 03 Jun 2019 20:26:10: 31000000 INFO @ Mon, 03 Jun 2019 20:26:10: 30000000 INFO @ Mon, 03 Jun 2019 20:26:11: 25000000 INFO @ Mon, 03 Jun 2019 20:26:17: 32000000 INFO @ Mon, 03 Jun 2019 20:26:18: 31000000 INFO @ Mon, 03 Jun 2019 20:26:21: 26000000 INFO @ Mon, 03 Jun 2019 20:26:25: 33000000 INFO @ Mon, 03 Jun 2019 20:26:25: 32000000 INFO @ Mon, 03 Jun 2019 20:26:31: 27000000 INFO @ Mon, 03 Jun 2019 20:26:32: 34000000 INFO @ Mon, 03 Jun 2019 20:26:33: 33000000 INFO @ Mon, 03 Jun 2019 20:26:39: 35000000 INFO @ Mon, 03 Jun 2019 20:26:40: 34000000 INFO @ Mon, 03 Jun 2019 20:26:41: 28000000 INFO @ Mon, 03 Jun 2019 20:26:46: 36000000 INFO @ Mon, 03 Jun 2019 20:26:48: 35000000 INFO @ Mon, 03 Jun 2019 20:26:51: 29000000 INFO @ Mon, 03 Jun 2019 20:26:54: 37000000 INFO @ Mon, 03 Jun 2019 20:26:56: 36000000 INFO @ Mon, 03 Jun 2019 20:27:00: 30000000 INFO @ Mon, 03 Jun 2019 20:27:01: 38000000 INFO @ Mon, 03 Jun 2019 20:27:04: 37000000 INFO @ Mon, 03 Jun 2019 20:27:08: 39000000 INFO @ Mon, 03 Jun 2019 20:27:10: 31000000 INFO @ Mon, 03 Jun 2019 20:27:12: 38000000 INFO @ Mon, 03 Jun 2019 20:27:15: 40000000 INFO @ Mon, 03 Jun 2019 20:27:19: 32000000 INFO @ Mon, 03 Jun 2019 20:27:19: 39000000 INFO @ Mon, 03 Jun 2019 20:27:22: 41000000 INFO @ Mon, 03 Jun 2019 20:27:27: 40000000 INFO @ Mon, 03 Jun 2019 20:27:28: 33000000 INFO @ Mon, 03 Jun 2019 20:27:30: 42000000 INFO @ Mon, 03 Jun 2019 20:27:34: 41000000 INFO @ Mon, 03 Jun 2019 20:27:37: 43000000 INFO @ Mon, 03 Jun 2019 20:27:38: 34000000 INFO @ Mon, 03 Jun 2019 20:27:42: 42000000 INFO @ Mon, 03 Jun 2019 20:27:44: 44000000 INFO @ Mon, 03 Jun 2019 20:27:47: 35000000 INFO @ Mon, 03 Jun 2019 20:27:49: 43000000 INFO @ Mon, 03 Jun 2019 20:27:51: 45000000 INFO @ Mon, 03 Jun 2019 20:27:56: 36000000 INFO @ Mon, 03 Jun 2019 20:27:57: 44000000 INFO @ Mon, 03 Jun 2019 20:27:59: 46000000 INFO @ Mon, 03 Jun 2019 20:28:04: 45000000 INFO @ Mon, 03 Jun 2019 20:28:06: 47000000 INFO @ Mon, 03 Jun 2019 20:28:06: 37000000 INFO @ Mon, 03 Jun 2019 20:28:12: 46000000 INFO @ Mon, 03 Jun 2019 20:28:13: 48000000 INFO @ Mon, 03 Jun 2019 20:28:15: 38000000 INFO @ Mon, 03 Jun 2019 20:28:19: 47000000 INFO @ Mon, 03 Jun 2019 20:28:20: 49000000 INFO @ Mon, 03 Jun 2019 20:28:24: 39000000 INFO @ Mon, 03 Jun 2019 20:28:27: 48000000 INFO @ Mon, 03 Jun 2019 20:28:28: 50000000 INFO @ Mon, 03 Jun 2019 20:28:34: 40000000 INFO @ Mon, 03 Jun 2019 20:28:34: 49000000 INFO @ Mon, 03 Jun 2019 20:28:37: 51000000 INFO @ Mon, 03 Jun 2019 20:28:42: 50000000 INFO @ Mon, 03 Jun 2019 20:28:44: 41000000 INFO @ Mon, 03 Jun 2019 20:28:45: 52000000 INFO @ Mon, 03 Jun 2019 20:28:50: 51000000 INFO @ Mon, 03 Jun 2019 20:28:54: 42000000 INFO @ Mon, 03 Jun 2019 20:28:54: 53000000 INFO @ Mon, 03 Jun 2019 20:28:58: 52000000 INFO @ Mon, 03 Jun 2019 20:29:02: 54000000 INFO @ Mon, 03 Jun 2019 20:29:04: 43000000 INFO @ Mon, 03 Jun 2019 20:29:06: 53000000 INFO @ Mon, 03 Jun 2019 20:29:11: 55000000 INFO @ Mon, 03 Jun 2019 20:29:13: 44000000 INFO @ Mon, 03 Jun 2019 20:29:14: 54000000 INFO @ Mon, 03 Jun 2019 20:29:19: 56000000 INFO @ Mon, 03 Jun 2019 20:29:21: 55000000 INFO @ Mon, 03 Jun 2019 20:29:22: #1 tag size is determined as 76 bps INFO @ Mon, 03 Jun 2019 20:29:22: #1 tag size = 76 INFO @ Mon, 03 Jun 2019 20:29:22: #1 total tags in treatment: 21106253 INFO @ Mon, 03 Jun 2019 20:29:22: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:29:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:29:23: #1 tags after filtering in treatment: 13640828 INFO @ Mon, 03 Jun 2019 20:29:23: #1 Redundant rate of treatment: 0.35 INFO @ Mon, 03 Jun 2019 20:29:23: #1 finished! INFO @ Mon, 03 Jun 2019 20:29:23: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:29:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:29:23: 45000000 INFO @ Mon, 03 Jun 2019 20:29:25: #2 number of paired peaks: 8319 INFO @ Mon, 03 Jun 2019 20:29:25: start model_add_line... INFO @ Mon, 03 Jun 2019 20:29:25: start X-correlation... INFO @ Mon, 03 Jun 2019 20:29:25: end of X-cor INFO @ Mon, 03 Jun 2019 20:29:25: #2 finished! INFO @ Mon, 03 Jun 2019 20:29:25: #2 predicted fragment length is 280 bps INFO @ Mon, 03 Jun 2019 20:29:25: #2 alternative fragment length(s) may be 280 bps INFO @ Mon, 03 Jun 2019 20:29:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.20_model.r INFO @ Mon, 03 Jun 2019 20:29:25: #3 Call peaks... INFO @ Mon, 03 Jun 2019 20:29:25: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 20:29:29: 56000000 INFO @ Mon, 03 Jun 2019 20:29:32: #1 tag size is determined as 76 bps INFO @ Mon, 03 Jun 2019 20:29:32: #1 tag size = 76 INFO @ Mon, 03 Jun 2019 20:29:32: #1 total tags in treatment: 21106253 INFO @ Mon, 03 Jun 2019 20:29:32: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:29:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:29:32: #1 tags after filtering in treatment: 13640828 INFO @ Mon, 03 Jun 2019 20:29:32: #1 Redundant rate of treatment: 0.35 INFO @ Mon, 03 Jun 2019 20:29:32: #1 finished! INFO @ Mon, 03 Jun 2019 20:29:32: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:29:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:29:33: 46000000 INFO @ Mon, 03 Jun 2019 20:29:35: #2 number of paired peaks: 8319 INFO @ Mon, 03 Jun 2019 20:29:35: start model_add_line... INFO @ Mon, 03 Jun 2019 20:29:35: start X-correlation... INFO @ Mon, 03 Jun 2019 20:29:35: end of X-cor INFO @ Mon, 03 Jun 2019 20:29:35: #2 finished! INFO @ Mon, 03 Jun 2019 20:29:35: #2 predicted fragment length is 280 bps INFO @ Mon, 03 Jun 2019 20:29:35: #2 alternative fragment length(s) may be 280 bps INFO @ Mon, 03 Jun 2019 20:29:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.10_model.r INFO @ Mon, 03 Jun 2019 20:29:35: #3 Call peaks... INFO @ Mon, 03 Jun 2019 20:29:35: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 20:29:42: 47000000 INFO @ Mon, 03 Jun 2019 20:29:51: 48000000 INFO @ Mon, 03 Jun 2019 20:30:01: 49000000 INFO @ Mon, 03 Jun 2019 20:30:10: 50000000 INFO @ Mon, 03 Jun 2019 20:30:19: 51000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 03 Jun 2019 20:30:26: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 20:30:29: 52000000 INFO @ Mon, 03 Jun 2019 20:30:32: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 20:30:39: 53000000 INFO @ Mon, 03 Jun 2019 20:30:50: 54000000 INFO @ Mon, 03 Jun 2019 20:30:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.20_peaks.xls INFO @ Mon, 03 Jun 2019 20:30:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 20:30:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.20_summits.bed INFO @ Mon, 03 Jun 2019 20:30:52: Done! pass1 - making usageList (14 chroms): 4 millis pass2 - checking and writing primary data (8217 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 20:30:53: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.10_peaks.xls INFO @ Mon, 03 Jun 2019 20:30:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 20:30:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.10_summits.bed INFO @ Mon, 03 Jun 2019 20:30:54: Done! pass1 - making usageList (14 chroms): 6 millis pass2 - checking and writing primary data (12268 records, 4 fields): 20 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 20:31:00: 55000000 INFO @ Mon, 03 Jun 2019 20:31:09: 56000000 INFO @ Mon, 03 Jun 2019 20:31:13: #1 tag size is determined as 76 bps INFO @ Mon, 03 Jun 2019 20:31:13: #1 tag size = 76 INFO @ Mon, 03 Jun 2019 20:31:13: #1 total tags in treatment: 21106253 INFO @ Mon, 03 Jun 2019 20:31:13: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 20:31:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 20:31:14: #1 tags after filtering in treatment: 13640828 INFO @ Mon, 03 Jun 2019 20:31:14: #1 Redundant rate of treatment: 0.35 INFO @ Mon, 03 Jun 2019 20:31:14: #1 finished! INFO @ Mon, 03 Jun 2019 20:31:14: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 20:31:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 20:31:16: #2 number of paired peaks: 8319 INFO @ Mon, 03 Jun 2019 20:31:16: start model_add_line... INFO @ Mon, 03 Jun 2019 20:31:16: start X-correlation... INFO @ Mon, 03 Jun 2019 20:31:16: end of X-cor INFO @ Mon, 03 Jun 2019 20:31:16: #2 finished! INFO @ Mon, 03 Jun 2019 20:31:16: #2 predicted fragment length is 280 bps INFO @ Mon, 03 Jun 2019 20:31:16: #2 alternative fragment length(s) may be 280 bps INFO @ Mon, 03 Jun 2019 20:31:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.05_model.r INFO @ Mon, 03 Jun 2019 20:31:16: #3 Call peaks... INFO @ Mon, 03 Jun 2019 20:31:16: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Mon, 03 Jun 2019 20:32:15: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 20:32:38: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.05_peaks.xls INFO @ Mon, 03 Jun 2019 20:32:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 20:32:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX264599/SRX264599.05_summits.bed INFO @ Mon, 03 Jun 2019 20:32:38: Done! pass1 - making usageList (14 chroms): 7 millis pass2 - checking and writing primary data (16032 records, 4 fields): 24 millis CompletedMACS2peakCalling