Job ID = 9730447 sra ファイルのダウンロード中... Completed: 959806K bytes transferred in 54 seconds (144057K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 28970111 spots for /home/okishinya/chipatlas/results/dm3/SRX2638361/SRR5341686.sra Written 28970111 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:42 28970111 reads; of these: 28970111 (100.00%) were unpaired; of these: 21862372 (75.47%) aligned 0 times 2694549 (9.30%) aligned exactly 1 time 4413190 (15.23%) aligned >1 times 24.53% overall alignment rate Time searching: 00:08:42 Overall time: 00:08:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 5667688 / 7107739 = 0.7974 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 03 Sep 2017 01:06:47: # Command line: callpeak -t SRX2638361.bam -f BAM -g dm -n SRX2638361.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2638361.20 # format = BAM # ChIP-seq file = ['SRX2638361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Sep 2017 01:06:47: # Command line: callpeak -t SRX2638361.bam -f BAM -g dm -n SRX2638361.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2638361.10 # format = BAM # ChIP-seq file = ['SRX2638361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Sep 2017 01:06:47: # Command line: callpeak -t SRX2638361.bam -f BAM -g dm -n SRX2638361.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2638361.05 # format = BAM # ChIP-seq file = ['SRX2638361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Sep 2017 01:06:47: #1 read tag files... INFO @ Sun, 03 Sep 2017 01:06:47: #1 read tag files... INFO @ Sun, 03 Sep 2017 01:06:47: #1 read tag files... INFO @ Sun, 03 Sep 2017 01:06:47: #1 read treatment tags... INFO @ Sun, 03 Sep 2017 01:06:47: #1 read treatment tags... INFO @ Sun, 03 Sep 2017 01:06:47: #1 read treatment tags... INFO @ Sun, 03 Sep 2017 01:06:54: 1000000 INFO @ Sun, 03 Sep 2017 01:06:54: 1000000 INFO @ Sun, 03 Sep 2017 01:06:54: 1000000 INFO @ Sun, 03 Sep 2017 01:06:57: #1 tag size is determined as 51 bps INFO @ Sun, 03 Sep 2017 01:06:57: #1 tag size = 51 INFO @ Sun, 03 Sep 2017 01:06:57: #1 total tags in treatment: 1440051 INFO @ Sun, 03 Sep 2017 01:06:57: #1 user defined the maximum tags... INFO @ Sun, 03 Sep 2017 01:06:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Sep 2017 01:06:57: #1 tags after filtering in treatment: 1440051 INFO @ Sun, 03 Sep 2017 01:06:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 03 Sep 2017 01:06:57: #1 finished! INFO @ Sun, 03 Sep 2017 01:06:57: #2 Build Peak Model... INFO @ Sun, 03 Sep 2017 01:06:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Sep 2017 01:06:57: #2 number of paired peaks: 2313 INFO @ Sun, 03 Sep 2017 01:06:57: start model_add_line... INFO @ Sun, 03 Sep 2017 01:06:57: start X-correlation... INFO @ Sun, 03 Sep 2017 01:06:57: end of X-cor INFO @ Sun, 03 Sep 2017 01:06:57: #2 finished! INFO @ Sun, 03 Sep 2017 01:06:57: #2 predicted fragment length is 60 bps INFO @ Sun, 03 Sep 2017 01:06:57: #2 alternative fragment length(s) may be 60 bps INFO @ Sun, 03 Sep 2017 01:06:57: #2.2 Generate R script for model : SRX2638361.05_model.r WARNING @ Sun, 03 Sep 2017 01:06:57: #2 Since the d (60) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 03 Sep 2017 01:06:57: #2 You may need to consider one of the other alternative d(s): 60 WARNING @ Sun, 03 Sep 2017 01:06:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 03 Sep 2017 01:06:57: #3 Call peaks... INFO @ Sun, 03 Sep 2017 01:06:57: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 03 Sep 2017 01:06:57: #1 tag size is determined as 51 bps INFO @ Sun, 03 Sep 2017 01:06:57: #1 tag size = 51 INFO @ Sun, 03 Sep 2017 01:06:57: #1 total tags in treatment: 1440051 INFO @ Sun, 03 Sep 2017 01:06:57: #1 user defined the maximum tags... INFO @ Sun, 03 Sep 2017 01:06:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Sep 2017 01:06:57: #1 tag size is determined as 51 bps INFO @ Sun, 03 Sep 2017 01:06:57: #1 tag size = 51 INFO @ Sun, 03 Sep 2017 01:06:57: #1 total tags in treatment: 1440051 INFO @ Sun, 03 Sep 2017 01:06:57: #1 user defined the maximum tags... INFO @ Sun, 03 Sep 2017 01:06:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Sep 2017 01:06:57: #1 tags after filtering in treatment: 1440051 INFO @ Sun, 03 Sep 2017 01:06:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 03 Sep 2017 01:06:57: #1 finished! INFO @ Sun, 03 Sep 2017 01:06:57: #2 Build Peak Model... INFO @ Sun, 03 Sep 2017 01:06:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Sep 2017 01:06:57: #1 tags after filtering in treatment: 1440051 INFO @ Sun, 03 Sep 2017 01:06:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 03 Sep 2017 01:06:57: #1 finished! INFO @ Sun, 03 Sep 2017 01:06:57: #2 Build Peak Model... INFO @ Sun, 03 Sep 2017 01:06:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Sep 2017 01:06:57: #2 number of paired peaks: 2313 INFO @ Sun, 03 Sep 2017 01:06:57: start model_add_line... INFO @ Sun, 03 Sep 2017 01:06:57: #2 number of paired peaks: 2313 INFO @ Sun, 03 Sep 2017 01:06:57: start model_add_line... INFO @ Sun, 03 Sep 2017 01:06:58: start X-correlation... INFO @ Sun, 03 Sep 2017 01:06:58: end of X-cor INFO @ Sun, 03 Sep 2017 01:06:58: #2 finished! INFO @ Sun, 03 Sep 2017 01:06:58: #2 predicted fragment length is 60 bps INFO @ Sun, 03 Sep 2017 01:06:58: #2 alternative fragment length(s) may be 60 bps INFO @ Sun, 03 Sep 2017 01:06:58: start X-correlation... INFO @ Sun, 03 Sep 2017 01:06:58: #2.2 Generate R script for model : SRX2638361.20_model.r INFO @ Sun, 03 Sep 2017 01:06:58: end of X-cor INFO @ Sun, 03 Sep 2017 01:06:58: #2 finished! INFO @ Sun, 03 Sep 2017 01:06:58: #2 predicted fragment length is 60 bps INFO @ Sun, 03 Sep 2017 01:06:58: #2 alternative fragment length(s) may be 60 bps INFO @ Sun, 03 Sep 2017 01:06:58: #2.2 Generate R script for model : SRX2638361.10_model.r WARNING @ Sun, 03 Sep 2017 01:06:58: #2 Since the d (60) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 03 Sep 2017 01:06:58: #2 You may need to consider one of the other alternative d(s): 60 WARNING @ Sun, 03 Sep 2017 01:06:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 03 Sep 2017 01:06:58: #3 Call peaks... INFO @ Sun, 03 Sep 2017 01:06:58: #3 Pre-compute pvalue-qvalue table... WARNING @ Sun, 03 Sep 2017 01:06:58: #2 Since the d (60) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 03 Sep 2017 01:06:58: #2 You may need to consider one of the other alternative d(s): 60 WARNING @ Sun, 03 Sep 2017 01:06:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 03 Sep 2017 01:06:58: #3 Call peaks... INFO @ Sun, 03 Sep 2017 01:06:58: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 03 Sep 2017 01:07:01: #3 Call peaks for each chromosome... INFO @ Sun, 03 Sep 2017 01:07:01: #3 Call peaks for each chromosome... INFO @ Sun, 03 Sep 2017 01:07:01: #3 Call peaks for each chromosome... INFO @ Sun, 03 Sep 2017 01:07:03: #4 Write output xls file... SRX2638361.05_peaks.xls INFO @ Sun, 03 Sep 2017 01:07:03: #4 Write peak in narrowPeak format file... SRX2638361.05_peaks.narrowPeak INFO @ Sun, 03 Sep 2017 01:07:03: #4 Write summits bed file... SRX2638361.05_summits.bed INFO @ Sun, 03 Sep 2017 01:07:03: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (2593 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Sun, 03 Sep 2017 01:07:03: #4 Write output xls file... SRX2638361.10_peaks.xls INFO @ Sun, 03 Sep 2017 01:07:03: #4 Write peak in narrowPeak format file... SRX2638361.10_peaks.narrowPeak INFO @ Sun, 03 Sep 2017 01:07:03: #4 Write summits bed file... SRX2638361.10_summits.bed INFO @ Sun, 03 Sep 2017 01:07:03: Done! INFO @ Sun, 03 Sep 2017 01:07:03: #4 Write output xls file... SRX2638361.20_peaks.xls INFO @ Sun, 03 Sep 2017 01:07:03: #4 Write peak in narrowPeak format file... SRX2638361.20_peaks.narrowPeak INFO @ Sun, 03 Sep 2017 01:07:03: #4 Write summits bed file... SRX2638361.20_summits.bed INFO @ Sun, 03 Sep 2017 01:07:03: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1773 records, 4 fields): 5 millis CompletedMACS2peakCalling pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (1304 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。