Job ID = 11171236 sra ファイルのダウンロード中... Completed: 483606K bytes transferred in 23 seconds (170131K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 25165879 spots for /home/okishinya/chipatlas/results/dm3/SRX2618555/SRR5319109.sra Written 25165879 spots for /home/okishinya/chipatlas/results/dm3/SRX2618555/SRR5319109.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:11:03 25165879 reads; of these: 25165879 (100.00%) were unpaired; of these: 815108 (3.24%) aligned 0 times 17447364 (69.33%) aligned exactly 1 time 6903407 (27.43%) aligned >1 times 96.76% overall alignment rate Time searching: 00:11:04 Overall time: 00:11:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2575672 / 24350771 = 0.1058 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:29:06: # Command line: callpeak -t SRX2618555.bam -f BAM -g dm -n SRX2618555.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2618555.10 # format = BAM # ChIP-seq file = ['SRX2618555.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:29:06: # Command line: callpeak -t SRX2618555.bam -f BAM -g dm -n SRX2618555.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2618555.05 # format = BAM # ChIP-seq file = ['SRX2618555.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:29:06: # Command line: callpeak -t SRX2618555.bam -f BAM -g dm -n SRX2618555.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2618555.20 # format = BAM # ChIP-seq file = ['SRX2618555.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:29:06: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:29:06: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:29:06: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:29:06: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:29:06: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:29:06: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:29:13: 1000000 INFO @ Sat, 08 Sep 2018 13:29:13: 1000000 INFO @ Sat, 08 Sep 2018 13:29:13: 1000000 INFO @ Sat, 08 Sep 2018 13:29:19: 2000000 INFO @ Sat, 08 Sep 2018 13:29:19: 2000000 INFO @ Sat, 08 Sep 2018 13:29:19: 2000000 INFO @ Sat, 08 Sep 2018 13:29:25: 3000000 INFO @ Sat, 08 Sep 2018 13:29:26: 3000000 INFO @ Sat, 08 Sep 2018 13:29:26: 3000000 INFO @ Sat, 08 Sep 2018 13:29:32: 4000000 INFO @ Sat, 08 Sep 2018 13:29:32: 4000000 INFO @ Sat, 08 Sep 2018 13:29:33: 4000000 INFO @ Sat, 08 Sep 2018 13:29:38: 5000000 INFO @ Sat, 08 Sep 2018 13:29:39: 5000000 INFO @ Sat, 08 Sep 2018 13:29:40: 5000000 INFO @ Sat, 08 Sep 2018 13:29:44: 6000000 INFO @ Sat, 08 Sep 2018 13:29:46: 6000000 INFO @ Sat, 08 Sep 2018 13:29:47: 6000000 INFO @ Sat, 08 Sep 2018 13:29:50: 7000000 INFO @ Sat, 08 Sep 2018 13:29:53: 7000000 INFO @ Sat, 08 Sep 2018 13:29:54: 7000000 INFO @ Sat, 08 Sep 2018 13:29:56: 8000000 INFO @ Sat, 08 Sep 2018 13:30:00: 8000000 INFO @ Sat, 08 Sep 2018 13:30:01: 8000000 INFO @ Sat, 08 Sep 2018 13:30:02: 9000000 INFO @ Sat, 08 Sep 2018 13:30:06: 9000000 INFO @ Sat, 08 Sep 2018 13:30:08: 10000000 INFO @ Sat, 08 Sep 2018 13:30:08: 9000000 INFO @ Sat, 08 Sep 2018 13:30:13: 10000000 INFO @ Sat, 08 Sep 2018 13:30:14: 11000000 INFO @ Sat, 08 Sep 2018 13:30:15: 10000000 INFO @ Sat, 08 Sep 2018 13:30:20: 11000000 INFO @ Sat, 08 Sep 2018 13:30:20: 12000000 INFO @ Sat, 08 Sep 2018 13:30:22: 11000000 INFO @ Sat, 08 Sep 2018 13:30:26: 12000000 INFO @ Sat, 08 Sep 2018 13:30:27: 13000000 INFO @ Sat, 08 Sep 2018 13:30:29: 12000000 INFO @ Sat, 08 Sep 2018 13:30:33: 14000000 INFO @ Sat, 08 Sep 2018 13:30:33: 13000000 INFO @ Sat, 08 Sep 2018 13:30:36: 13000000 INFO @ Sat, 08 Sep 2018 13:30:39: 15000000 INFO @ Sat, 08 Sep 2018 13:30:40: 14000000 INFO @ Sat, 08 Sep 2018 13:30:43: 14000000 INFO @ Sat, 08 Sep 2018 13:30:45: 16000000 INFO @ Sat, 08 Sep 2018 13:30:47: 15000000 INFO @ Sat, 08 Sep 2018 13:30:50: 15000000 INFO @ Sat, 08 Sep 2018 13:30:51: 17000000 INFO @ Sat, 08 Sep 2018 13:30:53: 16000000 INFO @ Sat, 08 Sep 2018 13:30:57: 18000000 INFO @ Sat, 08 Sep 2018 13:30:57: 16000000 INFO @ Sat, 08 Sep 2018 13:31:00: 17000000 INFO @ Sat, 08 Sep 2018 13:31:03: 19000000 INFO @ Sat, 08 Sep 2018 13:31:04: 17000000 INFO @ Sat, 08 Sep 2018 13:31:07: 18000000 INFO @ Sat, 08 Sep 2018 13:31:09: 20000000 INFO @ Sat, 08 Sep 2018 13:31:11: 18000000 INFO @ Sat, 08 Sep 2018 13:31:14: 19000000 INFO @ Sat, 08 Sep 2018 13:31:15: 21000000 INFO @ Sat, 08 Sep 2018 13:31:18: 19000000 INFO @ Sat, 08 Sep 2018 13:31:20: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:31:20: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:31:20: #1 total tags in treatment: 21775099 INFO @ Sat, 08 Sep 2018 13:31:20: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:31:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:31:20: 20000000 INFO @ Sat, 08 Sep 2018 13:31:21: #1 tags after filtering in treatment: 21775099 INFO @ Sat, 08 Sep 2018 13:31:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:31:21: #1 finished! INFO @ Sat, 08 Sep 2018 13:31:21: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:31:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:31:22: #2 number of paired peaks: 6 WARNING @ Sat, 08 Sep 2018 13:31:22: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:31:22: Process for pairing-model is terminated! cat: SRX2618555.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618555.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618555.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618555.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:31:25: 20000000 INFO @ Sat, 08 Sep 2018 13:31:27: 21000000 INFO @ Sat, 08 Sep 2018 13:31:32: 21000000 INFO @ Sat, 08 Sep 2018 13:31:32: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:31:32: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:31:32: #1 total tags in treatment: 21775099 INFO @ Sat, 08 Sep 2018 13:31:32: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:31:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:31:33: #1 tags after filtering in treatment: 21775099 INFO @ Sat, 08 Sep 2018 13:31:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:31:33: #1 finished! INFO @ Sat, 08 Sep 2018 13:31:33: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:31:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:31:35: #2 number of paired peaks: 6 WARNING @ Sat, 08 Sep 2018 13:31:35: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:31:35: Process for pairing-model is terminated! cat: SRX2618555.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618555.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618555.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618555.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:31:37: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:31:37: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:31:37: #1 total tags in treatment: 21775099 INFO @ Sat, 08 Sep 2018 13:31:37: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:31:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:31:38: #1 tags after filtering in treatment: 21775099 INFO @ Sat, 08 Sep 2018 13:31:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:31:38: #1 finished! INFO @ Sat, 08 Sep 2018 13:31:38: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:31:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:31:39: #2 number of paired peaks: 6 WARNING @ Sat, 08 Sep 2018 13:31:39: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:31:39: Process for pairing-model is terminated! cat: SRX2618555.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618555.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618555.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618555.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。