Job ID = 11171234 sra ファイルのダウンロード中... Completed: 540995K bytes transferred in 26 seconds (169185K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 28023752 spots for /home/okishinya/chipatlas/results/dm3/SRX2618553/SRR5319107.sra Written 28023752 spots for /home/okishinya/chipatlas/results/dm3/SRX2618553/SRR5319107.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:01 28023752 reads; of these: 28023752 (100.00%) were unpaired; of these: 835197 (2.98%) aligned 0 times 19542056 (69.73%) aligned exactly 1 time 7646499 (27.29%) aligned >1 times 97.02% overall alignment rate Time searching: 00:12:01 Overall time: 00:12:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3073942 / 27188555 = 0.1131 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:29:22: # Command line: callpeak -t SRX2618553.bam -f BAM -g dm -n SRX2618553.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2618553.10 # format = BAM # ChIP-seq file = ['SRX2618553.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:29:22: # Command line: callpeak -t SRX2618553.bam -f BAM -g dm -n SRX2618553.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2618553.20 # format = BAM # ChIP-seq file = ['SRX2618553.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:29:22: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:29:22: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:29:22: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:29:22: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:29:22: # Command line: callpeak -t SRX2618553.bam -f BAM -g dm -n SRX2618553.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2618553.05 # format = BAM # ChIP-seq file = ['SRX2618553.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:29:22: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:29:22: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:29:29: 1000000 INFO @ Sat, 08 Sep 2018 13:29:29: 1000000 INFO @ Sat, 08 Sep 2018 13:29:29: 1000000 INFO @ Sat, 08 Sep 2018 13:29:35: 2000000 INFO @ Sat, 08 Sep 2018 13:29:36: 2000000 INFO @ Sat, 08 Sep 2018 13:29:36: 2000000 INFO @ Sat, 08 Sep 2018 13:29:42: 3000000 INFO @ Sat, 08 Sep 2018 13:29:43: 3000000 INFO @ Sat, 08 Sep 2018 13:29:43: 3000000 INFO @ Sat, 08 Sep 2018 13:29:49: 4000000 INFO @ Sat, 08 Sep 2018 13:29:50: 4000000 INFO @ Sat, 08 Sep 2018 13:29:50: 4000000 INFO @ Sat, 08 Sep 2018 13:29:55: 5000000 INFO @ Sat, 08 Sep 2018 13:29:57: 5000000 INFO @ Sat, 08 Sep 2018 13:29:57: 5000000 INFO @ Sat, 08 Sep 2018 13:30:02: 6000000 INFO @ Sat, 08 Sep 2018 13:30:04: 6000000 INFO @ Sat, 08 Sep 2018 13:30:04: 6000000 INFO @ Sat, 08 Sep 2018 13:30:09: 7000000 INFO @ Sat, 08 Sep 2018 13:30:11: 7000000 INFO @ Sat, 08 Sep 2018 13:30:11: 7000000 INFO @ Sat, 08 Sep 2018 13:30:15: 8000000 INFO @ Sat, 08 Sep 2018 13:30:18: 8000000 INFO @ Sat, 08 Sep 2018 13:30:18: 8000000 INFO @ Sat, 08 Sep 2018 13:30:22: 9000000 INFO @ Sat, 08 Sep 2018 13:30:25: 9000000 INFO @ Sat, 08 Sep 2018 13:30:25: 9000000 INFO @ Sat, 08 Sep 2018 13:30:29: 10000000 INFO @ Sat, 08 Sep 2018 13:30:32: 10000000 INFO @ Sat, 08 Sep 2018 13:30:32: 10000000 INFO @ Sat, 08 Sep 2018 13:30:35: 11000000 INFO @ Sat, 08 Sep 2018 13:30:39: 11000000 INFO @ Sat, 08 Sep 2018 13:30:39: 11000000 INFO @ Sat, 08 Sep 2018 13:30:42: 12000000 INFO @ Sat, 08 Sep 2018 13:30:46: 12000000 INFO @ Sat, 08 Sep 2018 13:30:46: 12000000 INFO @ Sat, 08 Sep 2018 13:30:49: 13000000 INFO @ Sat, 08 Sep 2018 13:30:53: 13000000 INFO @ Sat, 08 Sep 2018 13:30:53: 13000000 INFO @ Sat, 08 Sep 2018 13:30:55: 14000000 INFO @ Sat, 08 Sep 2018 13:31:00: 14000000 INFO @ Sat, 08 Sep 2018 13:31:00: 14000000 INFO @ Sat, 08 Sep 2018 13:31:02: 15000000 INFO @ Sat, 08 Sep 2018 13:31:07: 15000000 INFO @ Sat, 08 Sep 2018 13:31:07: 15000000 INFO @ Sat, 08 Sep 2018 13:31:09: 16000000 INFO @ Sat, 08 Sep 2018 13:31:14: 16000000 INFO @ Sat, 08 Sep 2018 13:31:14: 16000000 INFO @ Sat, 08 Sep 2018 13:31:15: 17000000 INFO @ Sat, 08 Sep 2018 13:31:21: 17000000 INFO @ Sat, 08 Sep 2018 13:31:21: 17000000 INFO @ Sat, 08 Sep 2018 13:31:22: 18000000 INFO @ Sat, 08 Sep 2018 13:31:28: 18000000 INFO @ Sat, 08 Sep 2018 13:31:28: 18000000 INFO @ Sat, 08 Sep 2018 13:31:29: 19000000 INFO @ Sat, 08 Sep 2018 13:31:35: 19000000 INFO @ Sat, 08 Sep 2018 13:31:35: 19000000 INFO @ Sat, 08 Sep 2018 13:31:35: 20000000 INFO @ Sat, 08 Sep 2018 13:31:42: 20000000 INFO @ Sat, 08 Sep 2018 13:31:42: 20000000 INFO @ Sat, 08 Sep 2018 13:31:42: 21000000 INFO @ Sat, 08 Sep 2018 13:31:49: 22000000 INFO @ Sat, 08 Sep 2018 13:31:49: 21000000 INFO @ Sat, 08 Sep 2018 13:31:49: 21000000 INFO @ Sat, 08 Sep 2018 13:31:55: 23000000 INFO @ Sat, 08 Sep 2018 13:31:56: 22000000 INFO @ Sat, 08 Sep 2018 13:31:56: 22000000 INFO @ Sat, 08 Sep 2018 13:32:02: 24000000 INFO @ Sat, 08 Sep 2018 13:32:03: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:32:03: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:32:03: #1 total tags in treatment: 24114613 INFO @ Sat, 08 Sep 2018 13:32:03: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:32:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:32:03: 23000000 INFO @ Sat, 08 Sep 2018 13:32:03: 23000000 INFO @ Sat, 08 Sep 2018 13:32:03: #1 tags after filtering in treatment: 24114613 INFO @ Sat, 08 Sep 2018 13:32:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:32:03: #1 finished! INFO @ Sat, 08 Sep 2018 13:32:03: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:32:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:32:05: #2 number of paired peaks: 3 WARNING @ Sat, 08 Sep 2018 13:32:05: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:32:05: Process for pairing-model is terminated! cat: SRX2618553.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618553.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618553.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618553.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:32:10: 24000000 INFO @ Sat, 08 Sep 2018 13:32:10: 24000000 INFO @ Sat, 08 Sep 2018 13:32:11: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:32:11: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:32:11: #1 total tags in treatment: 24114613 INFO @ Sat, 08 Sep 2018 13:32:11: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:32:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:32:11: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:32:11: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:32:11: #1 total tags in treatment: 24114613 INFO @ Sat, 08 Sep 2018 13:32:11: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:32:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:32:11: #1 tags after filtering in treatment: 24114613 INFO @ Sat, 08 Sep 2018 13:32:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:32:11: #1 finished! INFO @ Sat, 08 Sep 2018 13:32:11: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:32:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:32:11: #1 tags after filtering in treatment: 24114613 INFO @ Sat, 08 Sep 2018 13:32:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:32:11: #1 finished! INFO @ Sat, 08 Sep 2018 13:32:11: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:32:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:32:13: #2 number of paired peaks: 3 WARNING @ Sat, 08 Sep 2018 13:32:13: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:32:13: Process for pairing-model is terminated! cat: SRX2618553.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618553.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618553.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618553.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:32:13: #2 number of paired peaks: 3 WARNING @ Sat, 08 Sep 2018 13:32:13: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:32:13: Process for pairing-model is terminated! cat: SRX2618553.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618553.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618553.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618553.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。