Job ID = 11171226 sra ファイルのダウンロード中... Completed: 475199K bytes transferred in 18 seconds (208634K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 19378345 spots for /home/okishinya/chipatlas/results/dm3/SRX2618546/SRR5319100.sra Written 19378345 spots for /home/okishinya/chipatlas/results/dm3/SRX2618546/SRR5319100.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:06 19378345 reads; of these: 19378345 (100.00%) were unpaired; of these: 1873619 (9.67%) aligned 0 times 11484806 (59.27%) aligned exactly 1 time 6019920 (31.07%) aligned >1 times 90.33% overall alignment rate Time searching: 00:08:07 Overall time: 00:08:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1610524 / 17504726 = 0.0920 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:18:44: # Command line: callpeak -t SRX2618546.bam -f BAM -g dm -n SRX2618546.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2618546.10 # format = BAM # ChIP-seq file = ['SRX2618546.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:18:44: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:18:44: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:18:44: # Command line: callpeak -t SRX2618546.bam -f BAM -g dm -n SRX2618546.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2618546.05 # format = BAM # ChIP-seq file = ['SRX2618546.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:18:44: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:18:44: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:18:44: # Command line: callpeak -t SRX2618546.bam -f BAM -g dm -n SRX2618546.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2618546.20 # format = BAM # ChIP-seq file = ['SRX2618546.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:18:44: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:18:44: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:18:51: 1000000 INFO @ Sat, 08 Sep 2018 13:18:51: 1000000 INFO @ Sat, 08 Sep 2018 13:18:51: 1000000 INFO @ Sat, 08 Sep 2018 13:18:57: 2000000 INFO @ Sat, 08 Sep 2018 13:18:58: 2000000 INFO @ Sat, 08 Sep 2018 13:18:58: 2000000 INFO @ Sat, 08 Sep 2018 13:19:04: 3000000 INFO @ Sat, 08 Sep 2018 13:19:04: 3000000 INFO @ Sat, 08 Sep 2018 13:19:04: 3000000 INFO @ Sat, 08 Sep 2018 13:19:11: 4000000 INFO @ Sat, 08 Sep 2018 13:19:11: 4000000 INFO @ Sat, 08 Sep 2018 13:19:11: 4000000 INFO @ Sat, 08 Sep 2018 13:19:17: 5000000 INFO @ Sat, 08 Sep 2018 13:19:18: 5000000 INFO @ Sat, 08 Sep 2018 13:19:18: 5000000 INFO @ Sat, 08 Sep 2018 13:19:24: 6000000 INFO @ Sat, 08 Sep 2018 13:19:25: 6000000 INFO @ Sat, 08 Sep 2018 13:19:25: 6000000 INFO @ Sat, 08 Sep 2018 13:19:31: 7000000 INFO @ Sat, 08 Sep 2018 13:19:31: 7000000 INFO @ Sat, 08 Sep 2018 13:19:31: 7000000 INFO @ Sat, 08 Sep 2018 13:19:37: 8000000 INFO @ Sat, 08 Sep 2018 13:19:38: 8000000 INFO @ Sat, 08 Sep 2018 13:19:38: 8000000 INFO @ Sat, 08 Sep 2018 13:19:44: 9000000 INFO @ Sat, 08 Sep 2018 13:19:45: 9000000 INFO @ Sat, 08 Sep 2018 13:19:45: 9000000 INFO @ Sat, 08 Sep 2018 13:19:50: 10000000 INFO @ Sat, 08 Sep 2018 13:19:52: 10000000 INFO @ Sat, 08 Sep 2018 13:19:52: 10000000 INFO @ Sat, 08 Sep 2018 13:19:57: 11000000 INFO @ Sat, 08 Sep 2018 13:19:58: 11000000 INFO @ Sat, 08 Sep 2018 13:19:58: 11000000 INFO @ Sat, 08 Sep 2018 13:20:04: 12000000 INFO @ Sat, 08 Sep 2018 13:20:05: 12000000 INFO @ Sat, 08 Sep 2018 13:20:05: 12000000 INFO @ Sat, 08 Sep 2018 13:20:10: 13000000 INFO @ Sat, 08 Sep 2018 13:20:12: 13000000 INFO @ Sat, 08 Sep 2018 13:20:12: 13000000 INFO @ Sat, 08 Sep 2018 13:20:17: 14000000 INFO @ Sat, 08 Sep 2018 13:20:18: 14000000 INFO @ Sat, 08 Sep 2018 13:20:19: 14000000 INFO @ Sat, 08 Sep 2018 13:20:23: 15000000 INFO @ Sat, 08 Sep 2018 13:20:25: 15000000 INFO @ Sat, 08 Sep 2018 13:20:25: 15000000 INFO @ Sat, 08 Sep 2018 13:20:29: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:20:29: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:20:29: #1 total tags in treatment: 15894202 INFO @ Sat, 08 Sep 2018 13:20:29: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:20:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:20:30: #1 tags after filtering in treatment: 15894202 INFO @ Sat, 08 Sep 2018 13:20:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:20:30: #1 finished! INFO @ Sat, 08 Sep 2018 13:20:30: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:20:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:20:31: #2 number of paired peaks: 80 WARNING @ Sat, 08 Sep 2018 13:20:31: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:20:31: Process for pairing-model is terminated! cat: SRX2618546.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618546.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618546.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618546.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:20:31: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:20:31: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:20:31: #1 total tags in treatment: 15894202 INFO @ Sat, 08 Sep 2018 13:20:31: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:20:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:20:31: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:20:31: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:20:31: #1 total tags in treatment: 15894202 INFO @ Sat, 08 Sep 2018 13:20:31: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:20:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:20:32: #1 tags after filtering in treatment: 15894202 INFO @ Sat, 08 Sep 2018 13:20:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:20:32: #1 finished! INFO @ Sat, 08 Sep 2018 13:20:32: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:20:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:20:32: #1 tags after filtering in treatment: 15894202 INFO @ Sat, 08 Sep 2018 13:20:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:20:32: #1 finished! INFO @ Sat, 08 Sep 2018 13:20:32: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:20:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:20:33: #2 number of paired peaks: 80 WARNING @ Sat, 08 Sep 2018 13:20:33: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:20:33: Process for pairing-model is terminated! INFO @ Sat, 08 Sep 2018 13:20:33: #2 number of paired peaks: 80 WARNING @ Sat, 08 Sep 2018 13:20:33: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:20:33: Process for pairing-model is terminated! cat: SRX2618546.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX2618546.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618546.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618546.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618546.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618546.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618546.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618546.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。