Job ID = 11171224 sra ファイルのダウンロード中... Completed: 500487K bytes transferred in 24 seconds (170772K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 20448975 spots for /home/okishinya/chipatlas/results/dm3/SRX2618544/SRR5319098.sra Written 20448975 spots for /home/okishinya/chipatlas/results/dm3/SRX2618544/SRR5319098.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:28 20448975 reads; of these: 20448975 (100.00%) were unpaired; of these: 1894852 (9.27%) aligned 0 times 12178203 (59.55%) aligned exactly 1 time 6375920 (31.18%) aligned >1 times 90.73% overall alignment rate Time searching: 00:08:28 Overall time: 00:08:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1630230 / 18554123 = 0.0879 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:18:55: # Command line: callpeak -t SRX2618544.bam -f BAM -g dm -n SRX2618544.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2618544.20 # format = BAM # ChIP-seq file = ['SRX2618544.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:18:55: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:18:55: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:18:55: # Command line: callpeak -t SRX2618544.bam -f BAM -g dm -n SRX2618544.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2618544.05 # format = BAM # ChIP-seq file = ['SRX2618544.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:18:55: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:18:55: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:18:55: # Command line: callpeak -t SRX2618544.bam -f BAM -g dm -n SRX2618544.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2618544.10 # format = BAM # ChIP-seq file = ['SRX2618544.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:18:55: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:18:55: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:19:02: 1000000 INFO @ Sat, 08 Sep 2018 13:19:02: 1000000 INFO @ Sat, 08 Sep 2018 13:19:02: 1000000 INFO @ Sat, 08 Sep 2018 13:19:08: 2000000 INFO @ Sat, 08 Sep 2018 13:19:08: 2000000 INFO @ Sat, 08 Sep 2018 13:19:08: 2000000 INFO @ Sat, 08 Sep 2018 13:19:14: 3000000 INFO @ Sat, 08 Sep 2018 13:19:15: 3000000 INFO @ Sat, 08 Sep 2018 13:19:15: 3000000 INFO @ Sat, 08 Sep 2018 13:19:21: 4000000 INFO @ Sat, 08 Sep 2018 13:19:21: 4000000 INFO @ Sat, 08 Sep 2018 13:19:22: 4000000 INFO @ Sat, 08 Sep 2018 13:19:27: 5000000 INFO @ Sat, 08 Sep 2018 13:19:28: 5000000 INFO @ Sat, 08 Sep 2018 13:19:28: 5000000 INFO @ Sat, 08 Sep 2018 13:19:33: 6000000 INFO @ Sat, 08 Sep 2018 13:19:34: 6000000 INFO @ Sat, 08 Sep 2018 13:19:35: 6000000 INFO @ Sat, 08 Sep 2018 13:19:40: 7000000 INFO @ Sat, 08 Sep 2018 13:19:41: 7000000 INFO @ Sat, 08 Sep 2018 13:19:42: 7000000 INFO @ Sat, 08 Sep 2018 13:19:46: 8000000 INFO @ Sat, 08 Sep 2018 13:19:48: 8000000 INFO @ Sat, 08 Sep 2018 13:19:49: 8000000 INFO @ Sat, 08 Sep 2018 13:19:52: 9000000 INFO @ Sat, 08 Sep 2018 13:19:54: 9000000 INFO @ Sat, 08 Sep 2018 13:19:56: 9000000 INFO @ Sat, 08 Sep 2018 13:19:59: 10000000 INFO @ Sat, 08 Sep 2018 13:20:01: 10000000 INFO @ Sat, 08 Sep 2018 13:20:02: 10000000 INFO @ Sat, 08 Sep 2018 13:20:05: 11000000 INFO @ Sat, 08 Sep 2018 13:20:08: 11000000 INFO @ Sat, 08 Sep 2018 13:20:09: 11000000 INFO @ Sat, 08 Sep 2018 13:20:11: 12000000 INFO @ Sat, 08 Sep 2018 13:20:14: 12000000 INFO @ Sat, 08 Sep 2018 13:20:16: 12000000 INFO @ Sat, 08 Sep 2018 13:20:17: 13000000 INFO @ Sat, 08 Sep 2018 13:20:21: 13000000 INFO @ Sat, 08 Sep 2018 13:20:23: 13000000 INFO @ Sat, 08 Sep 2018 13:20:24: 14000000 INFO @ Sat, 08 Sep 2018 13:20:28: 14000000 INFO @ Sat, 08 Sep 2018 13:20:30: 14000000 INFO @ Sat, 08 Sep 2018 13:20:30: 15000000 INFO @ Sat, 08 Sep 2018 13:20:34: 15000000 INFO @ Sat, 08 Sep 2018 13:20:36: 16000000 INFO @ Sat, 08 Sep 2018 13:20:36: 15000000 INFO @ Sat, 08 Sep 2018 13:20:41: 16000000 INFO @ Sat, 08 Sep 2018 13:20:42: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:20:42: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:20:42: #1 total tags in treatment: 16923893 INFO @ Sat, 08 Sep 2018 13:20:42: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:20:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:20:43: #1 tags after filtering in treatment: 16923893 INFO @ Sat, 08 Sep 2018 13:20:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:20:43: #1 finished! INFO @ Sat, 08 Sep 2018 13:20:43: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:20:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:20:43: 16000000 INFO @ Sat, 08 Sep 2018 13:20:44: #2 number of paired peaks: 78 WARNING @ Sat, 08 Sep 2018 13:20:44: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:20:44: Process for pairing-model is terminated! cat: SRX2618544.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618544.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618544.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618544.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:20:47: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:20:47: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:20:47: #1 total tags in treatment: 16923893 INFO @ Sat, 08 Sep 2018 13:20:47: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:20:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:20:47: #1 tags after filtering in treatment: 16923893 INFO @ Sat, 08 Sep 2018 13:20:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:20:47: #1 finished! INFO @ Sat, 08 Sep 2018 13:20:47: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:20:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:20:49: #2 number of paired peaks: 78 WARNING @ Sat, 08 Sep 2018 13:20:49: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:20:49: Process for pairing-model is terminated! cat: SRX2618544.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618544.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618544.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618544.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:20:50: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:20:50: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:20:50: #1 total tags in treatment: 16923893 INFO @ Sat, 08 Sep 2018 13:20:50: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:20:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:20:50: #1 tags after filtering in treatment: 16923893 INFO @ Sat, 08 Sep 2018 13:20:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:20:50: #1 finished! INFO @ Sat, 08 Sep 2018 13:20:50: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:20:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:20:51: #2 number of paired peaks: 78 WARNING @ Sat, 08 Sep 2018 13:20:51: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:20:51: Process for pairing-model is terminated! cat: SRX2618544.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618544.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618544.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618544.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。