Job ID = 11171207 sra ファイルのダウンロード中... Completed: 885174K bytes transferred in 35 seconds (207121K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 46095103 spots for /home/okishinya/chipatlas/results/dm3/SRX2618517/SRR5319071.sra Written 46095103 spots for /home/okishinya/chipatlas/results/dm3/SRX2618517/SRR5319071.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:02 46095103 reads; of these: 46095103 (100.00%) were unpaired; of these: 16869167 (36.60%) aligned 0 times 22085109 (47.91%) aligned exactly 1 time 7140827 (15.49%) aligned >1 times 63.40% overall alignment rate Time searching: 00:13:02 Overall time: 00:13:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3921542 / 29225936 = 0.1342 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:18:49: # Command line: callpeak -t SRX2618517.bam -f BAM -g dm -n SRX2618517.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2618517.05 # format = BAM # ChIP-seq file = ['SRX2618517.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:18:49: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:18:49: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:18:49: # Command line: callpeak -t SRX2618517.bam -f BAM -g dm -n SRX2618517.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2618517.20 # format = BAM # ChIP-seq file = ['SRX2618517.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:18:49: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:18:49: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:18:49: # Command line: callpeak -t SRX2618517.bam -f BAM -g dm -n SRX2618517.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2618517.10 # format = BAM # ChIP-seq file = ['SRX2618517.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:18:49: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:18:49: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:18:55: 1000000 INFO @ Sat, 08 Sep 2018 13:18:55: 1000000 INFO @ Sat, 08 Sep 2018 13:18:55: 1000000 INFO @ Sat, 08 Sep 2018 13:19:02: 2000000 INFO @ Sat, 08 Sep 2018 13:19:02: 2000000 INFO @ Sat, 08 Sep 2018 13:19:02: 2000000 INFO @ Sat, 08 Sep 2018 13:19:08: 3000000 INFO @ Sat, 08 Sep 2018 13:19:08: 3000000 INFO @ Sat, 08 Sep 2018 13:19:08: 3000000 INFO @ Sat, 08 Sep 2018 13:19:15: 4000000 INFO @ Sat, 08 Sep 2018 13:19:15: 4000000 INFO @ Sat, 08 Sep 2018 13:19:15: 4000000 INFO @ Sat, 08 Sep 2018 13:19:21: 5000000 INFO @ Sat, 08 Sep 2018 13:19:21: 5000000 INFO @ Sat, 08 Sep 2018 13:19:22: 5000000 INFO @ Sat, 08 Sep 2018 13:19:27: 6000000 INFO @ Sat, 08 Sep 2018 13:19:27: 6000000 INFO @ Sat, 08 Sep 2018 13:19:28: 6000000 INFO @ Sat, 08 Sep 2018 13:19:34: 7000000 INFO @ Sat, 08 Sep 2018 13:19:34: 7000000 INFO @ Sat, 08 Sep 2018 13:19:35: 7000000 INFO @ Sat, 08 Sep 2018 13:19:40: 8000000 INFO @ Sat, 08 Sep 2018 13:19:40: 8000000 INFO @ Sat, 08 Sep 2018 13:19:41: 8000000 INFO @ Sat, 08 Sep 2018 13:19:46: 9000000 INFO @ Sat, 08 Sep 2018 13:19:46: 9000000 INFO @ Sat, 08 Sep 2018 13:19:48: 9000000 INFO @ Sat, 08 Sep 2018 13:19:53: 10000000 INFO @ Sat, 08 Sep 2018 13:19:53: 10000000 INFO @ Sat, 08 Sep 2018 13:19:55: 10000000 INFO @ Sat, 08 Sep 2018 13:19:59: 11000000 INFO @ Sat, 08 Sep 2018 13:19:59: 11000000 INFO @ Sat, 08 Sep 2018 13:20:01: 11000000 INFO @ Sat, 08 Sep 2018 13:20:06: 12000000 INFO @ Sat, 08 Sep 2018 13:20:06: 12000000 INFO @ Sat, 08 Sep 2018 13:20:08: 12000000 INFO @ Sat, 08 Sep 2018 13:20:12: 13000000 INFO @ Sat, 08 Sep 2018 13:20:12: 13000000 INFO @ Sat, 08 Sep 2018 13:20:14: 13000000 INFO @ Sat, 08 Sep 2018 13:20:18: 14000000 INFO @ Sat, 08 Sep 2018 13:20:19: 14000000 INFO @ Sat, 08 Sep 2018 13:20:21: 14000000 INFO @ Sat, 08 Sep 2018 13:20:24: 15000000 INFO @ Sat, 08 Sep 2018 13:20:25: 15000000 INFO @ Sat, 08 Sep 2018 13:20:28: 15000000 INFO @ Sat, 08 Sep 2018 13:20:31: 16000000 INFO @ Sat, 08 Sep 2018 13:20:32: 16000000 INFO @ Sat, 08 Sep 2018 13:20:35: 16000000 INFO @ Sat, 08 Sep 2018 13:20:37: 17000000 INFO @ Sat, 08 Sep 2018 13:20:39: 17000000 INFO @ Sat, 08 Sep 2018 13:20:41: 17000000 INFO @ Sat, 08 Sep 2018 13:20:43: 18000000 INFO @ Sat, 08 Sep 2018 13:20:45: 18000000 INFO @ Sat, 08 Sep 2018 13:20:48: 18000000 INFO @ Sat, 08 Sep 2018 13:20:50: 19000000 INFO @ Sat, 08 Sep 2018 13:20:51: 19000000 INFO @ Sat, 08 Sep 2018 13:20:55: 19000000 INFO @ Sat, 08 Sep 2018 13:20:57: 20000000 INFO @ Sat, 08 Sep 2018 13:20:58: 20000000 INFO @ Sat, 08 Sep 2018 13:21:02: 20000000 INFO @ Sat, 08 Sep 2018 13:21:03: 21000000 INFO @ Sat, 08 Sep 2018 13:21:04: 21000000 INFO @ Sat, 08 Sep 2018 13:21:09: 21000000 INFO @ Sat, 08 Sep 2018 13:21:10: 22000000 INFO @ Sat, 08 Sep 2018 13:21:10: 22000000 INFO @ Sat, 08 Sep 2018 13:21:16: 22000000 INFO @ Sat, 08 Sep 2018 13:21:16: 23000000 INFO @ Sat, 08 Sep 2018 13:21:17: 23000000 INFO @ Sat, 08 Sep 2018 13:21:22: 23000000 INFO @ Sat, 08 Sep 2018 13:21:23: 24000000 INFO @ Sat, 08 Sep 2018 13:21:23: 24000000 INFO @ Sat, 08 Sep 2018 13:21:29: 25000000 INFO @ Sat, 08 Sep 2018 13:21:29: 24000000 INFO @ Sat, 08 Sep 2018 13:21:30: 25000000 INFO @ Sat, 08 Sep 2018 13:21:31: #1 tag size is determined as 49 bps INFO @ Sat, 08 Sep 2018 13:21:31: #1 tag size = 49 INFO @ Sat, 08 Sep 2018 13:21:31: #1 total tags in treatment: 25304394 INFO @ Sat, 08 Sep 2018 13:21:31: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:21:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:21:32: #1 tag size is determined as 49 bps INFO @ Sat, 08 Sep 2018 13:21:32: #1 tag size = 49 INFO @ Sat, 08 Sep 2018 13:21:32: #1 total tags in treatment: 25304394 INFO @ Sat, 08 Sep 2018 13:21:32: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:21:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:21:32: #1 tags after filtering in treatment: 25304394 INFO @ Sat, 08 Sep 2018 13:21:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:21:32: #1 finished! INFO @ Sat, 08 Sep 2018 13:21:32: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:21:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:21:32: #1 tags after filtering in treatment: 25304394 INFO @ Sat, 08 Sep 2018 13:21:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:21:32: #1 finished! INFO @ Sat, 08 Sep 2018 13:21:32: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:21:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:21:33: #2 number of paired peaks: 53 WARNING @ Sat, 08 Sep 2018 13:21:33: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:21:33: Process for pairing-model is terminated! cat: SRX2618517.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618517.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618517.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618517.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:21:34: #2 number of paired peaks: 53 WARNING @ Sat, 08 Sep 2018 13:21:34: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:21:34: Process for pairing-model is terminated! cat: SRX2618517.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618517.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618517.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618517.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:21:36: 25000000 INFO @ Sat, 08 Sep 2018 13:21:38: #1 tag size is determined as 49 bps INFO @ Sat, 08 Sep 2018 13:21:38: #1 tag size = 49 INFO @ Sat, 08 Sep 2018 13:21:38: #1 total tags in treatment: 25304394 INFO @ Sat, 08 Sep 2018 13:21:38: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:21:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:21:38: #1 tags after filtering in treatment: 25304394 INFO @ Sat, 08 Sep 2018 13:21:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:21:38: #1 finished! INFO @ Sat, 08 Sep 2018 13:21:38: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:21:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:21:40: #2 number of paired peaks: 53 WARNING @ Sat, 08 Sep 2018 13:21:40: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:21:40: Process for pairing-model is terminated! cat: SRX2618517.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618517.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618517.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618517.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。