Job ID = 11171198 sra ファイルのダウンロード中... Completed: 875797K bytes transferred in 23 seconds (304977K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 45397592 spots for /home/okishinya/chipatlas/results/dm3/SRX2618511/SRR5319065.sra Written 45397592 spots for /home/okishinya/chipatlas/results/dm3/SRX2618511/SRR5319065.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:22 45397592 reads; of these: 45397592 (100.00%) were unpaired; of these: 14569739 (32.09%) aligned 0 times 23739263 (52.29%) aligned exactly 1 time 7088590 (15.61%) aligned >1 times 67.91% overall alignment rate Time searching: 00:13:22 Overall time: 00:13:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4015460 / 30827853 = 0.1303 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:10:15: # Command line: callpeak -t SRX2618511.bam -f BAM -g dm -n SRX2618511.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2618511.20 # format = BAM # ChIP-seq file = ['SRX2618511.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:10:15: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:10:15: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:10:15: # Command line: callpeak -t SRX2618511.bam -f BAM -g dm -n SRX2618511.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2618511.05 # format = BAM # ChIP-seq file = ['SRX2618511.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:10:15: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:10:15: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:10:15: # Command line: callpeak -t SRX2618511.bam -f BAM -g dm -n SRX2618511.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2618511.10 # format = BAM # ChIP-seq file = ['SRX2618511.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:10:15: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:10:15: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:10:21: 1000000 INFO @ Sat, 08 Sep 2018 13:10:21: 1000000 INFO @ Sat, 08 Sep 2018 13:10:21: 1000000 INFO @ Sat, 08 Sep 2018 13:10:27: 2000000 INFO @ Sat, 08 Sep 2018 13:10:28: 2000000 INFO @ Sat, 08 Sep 2018 13:10:28: 2000000 INFO @ Sat, 08 Sep 2018 13:10:33: 3000000 INFO @ Sat, 08 Sep 2018 13:10:34: 3000000 INFO @ Sat, 08 Sep 2018 13:10:34: 3000000 INFO @ Sat, 08 Sep 2018 13:10:40: 4000000 INFO @ Sat, 08 Sep 2018 13:10:40: 4000000 INFO @ Sat, 08 Sep 2018 13:10:41: 4000000 INFO @ Sat, 08 Sep 2018 13:10:46: 5000000 INFO @ Sat, 08 Sep 2018 13:10:47: 5000000 INFO @ Sat, 08 Sep 2018 13:10:47: 5000000 INFO @ Sat, 08 Sep 2018 13:10:53: 6000000 INFO @ Sat, 08 Sep 2018 13:10:53: 6000000 INFO @ Sat, 08 Sep 2018 13:10:54: 6000000 INFO @ Sat, 08 Sep 2018 13:10:59: 7000000 INFO @ Sat, 08 Sep 2018 13:11:00: 7000000 INFO @ Sat, 08 Sep 2018 13:11:01: 7000000 INFO @ Sat, 08 Sep 2018 13:11:06: 8000000 INFO @ Sat, 08 Sep 2018 13:11:06: 8000000 INFO @ Sat, 08 Sep 2018 13:11:08: 8000000 INFO @ Sat, 08 Sep 2018 13:11:12: 9000000 INFO @ Sat, 08 Sep 2018 13:11:12: 9000000 INFO @ Sat, 08 Sep 2018 13:11:15: 9000000 INFO @ Sat, 08 Sep 2018 13:11:19: 10000000 INFO @ Sat, 08 Sep 2018 13:11:19: 10000000 INFO @ Sat, 08 Sep 2018 13:11:22: 10000000 INFO @ Sat, 08 Sep 2018 13:11:25: 11000000 INFO @ Sat, 08 Sep 2018 13:11:25: 11000000 INFO @ Sat, 08 Sep 2018 13:11:29: 11000000 INFO @ Sat, 08 Sep 2018 13:11:32: 12000000 INFO @ Sat, 08 Sep 2018 13:11:32: 12000000 INFO @ Sat, 08 Sep 2018 13:11:36: 12000000 INFO @ Sat, 08 Sep 2018 13:11:39: 13000000 INFO @ Sat, 08 Sep 2018 13:11:39: 13000000 INFO @ Sat, 08 Sep 2018 13:11:43: 13000000 INFO @ Sat, 08 Sep 2018 13:11:45: 14000000 INFO @ Sat, 08 Sep 2018 13:11:46: 14000000 INFO @ Sat, 08 Sep 2018 13:11:50: 14000000 INFO @ Sat, 08 Sep 2018 13:11:52: 15000000 INFO @ Sat, 08 Sep 2018 13:11:52: 15000000 INFO @ Sat, 08 Sep 2018 13:11:57: 15000000 INFO @ Sat, 08 Sep 2018 13:11:58: 16000000 INFO @ Sat, 08 Sep 2018 13:11:58: 16000000 INFO @ Sat, 08 Sep 2018 13:12:04: 16000000 INFO @ Sat, 08 Sep 2018 13:12:05: 17000000 INFO @ Sat, 08 Sep 2018 13:12:05: 17000000 INFO @ Sat, 08 Sep 2018 13:12:11: 17000000 INFO @ Sat, 08 Sep 2018 13:12:11: 18000000 INFO @ Sat, 08 Sep 2018 13:12:11: 18000000 INFO @ Sat, 08 Sep 2018 13:12:18: 19000000 INFO @ Sat, 08 Sep 2018 13:12:18: 18000000 INFO @ Sat, 08 Sep 2018 13:12:18: 19000000 INFO @ Sat, 08 Sep 2018 13:12:24: 20000000 INFO @ Sat, 08 Sep 2018 13:12:25: 20000000 INFO @ Sat, 08 Sep 2018 13:12:25: 19000000 INFO @ Sat, 08 Sep 2018 13:12:31: 21000000 INFO @ Sat, 08 Sep 2018 13:12:31: 21000000 INFO @ Sat, 08 Sep 2018 13:12:32: 20000000 INFO @ Sat, 08 Sep 2018 13:12:37: 22000000 INFO @ Sat, 08 Sep 2018 13:12:38: 22000000 INFO @ Sat, 08 Sep 2018 13:12:39: 21000000 INFO @ Sat, 08 Sep 2018 13:12:43: 23000000 INFO @ Sat, 08 Sep 2018 13:12:45: 23000000 INFO @ Sat, 08 Sep 2018 13:12:46: 22000000 INFO @ Sat, 08 Sep 2018 13:12:50: 24000000 INFO @ Sat, 08 Sep 2018 13:12:51: 24000000 INFO @ Sat, 08 Sep 2018 13:12:53: 23000000 INFO @ Sat, 08 Sep 2018 13:12:56: 25000000 INFO @ Sat, 08 Sep 2018 13:12:58: 25000000 INFO @ Sat, 08 Sep 2018 13:13:00: 24000000 INFO @ Sat, 08 Sep 2018 13:13:02: 26000000 INFO @ Sat, 08 Sep 2018 13:13:04: 26000000 INFO @ Sat, 08 Sep 2018 13:13:07: 25000000 INFO @ Sat, 08 Sep 2018 13:13:08: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:13:08: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:13:08: #1 total tags in treatment: 26812393 INFO @ Sat, 08 Sep 2018 13:13:08: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:13:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:13:08: #1 tags after filtering in treatment: 26812393 INFO @ Sat, 08 Sep 2018 13:13:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:13:08: #1 finished! INFO @ Sat, 08 Sep 2018 13:13:08: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:13:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:13:10: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:13:10: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:13:10: #1 total tags in treatment: 26812393 INFO @ Sat, 08 Sep 2018 13:13:10: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:13:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:13:10: #2 number of paired peaks: 34 WARNING @ Sat, 08 Sep 2018 13:13:10: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:13:10: Process for pairing-model is terminated! cat: SRX2618511.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618511.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618511.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618511.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:13:10: #1 tags after filtering in treatment: 26812393 INFO @ Sat, 08 Sep 2018 13:13:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:13:10: #1 finished! INFO @ Sat, 08 Sep 2018 13:13:10: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:13:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:13:12: #2 number of paired peaks: 34 WARNING @ Sat, 08 Sep 2018 13:13:12: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:13:12: Process for pairing-model is terminated! cat: SRX2618511.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618511.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618511.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618511.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:13:13: 26000000 INFO @ Sat, 08 Sep 2018 13:13:19: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:13:19: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:13:19: #1 total tags in treatment: 26812393 INFO @ Sat, 08 Sep 2018 13:13:19: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:13:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:13:19: #1 tags after filtering in treatment: 26812393 INFO @ Sat, 08 Sep 2018 13:13:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:13:19: #1 finished! INFO @ Sat, 08 Sep 2018 13:13:19: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:13:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:13:21: #2 number of paired peaks: 34 WARNING @ Sat, 08 Sep 2018 13:13:21: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:13:21: Process for pairing-model is terminated! cat: SRX2618511.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618511.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618511.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618511.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。