Job ID = 11171208 sra ファイルのダウンロード中... Completed: 839886K bytes transferred in 34 seconds (202076K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 43119923 spots for /home/okishinya/chipatlas/results/dm3/SRX2618505/SRR5319059.sra Written 43119923 spots for /home/okishinya/chipatlas/results/dm3/SRX2618505/SRR5319059.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:35 43119923 reads; of these: 43119923 (100.00%) were unpaired; of these: 8898570 (20.64%) aligned 0 times 31174369 (72.30%) aligned exactly 1 time 3046984 (7.07%) aligned >1 times 79.36% overall alignment rate Time searching: 00:10:35 Overall time: 00:10:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4682353 / 34221353 = 0.1368 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:17:35: # Command line: callpeak -t SRX2618505.bam -f BAM -g dm -n SRX2618505.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2618505.20 # format = BAM # ChIP-seq file = ['SRX2618505.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:17:35: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:17:35: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:17:35: # Command line: callpeak -t SRX2618505.bam -f BAM -g dm -n SRX2618505.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2618505.10 # format = BAM # ChIP-seq file = ['SRX2618505.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:17:35: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:17:35: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:17:35: # Command line: callpeak -t SRX2618505.bam -f BAM -g dm -n SRX2618505.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2618505.05 # format = BAM # ChIP-seq file = ['SRX2618505.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:17:35: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:17:35: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:17:41: 1000000 INFO @ Sat, 08 Sep 2018 13:17:41: 1000000 INFO @ Sat, 08 Sep 2018 13:17:41: 1000000 INFO @ Sat, 08 Sep 2018 13:17:47: 2000000 INFO @ Sat, 08 Sep 2018 13:17:48: 2000000 INFO @ Sat, 08 Sep 2018 13:17:48: 2000000 INFO @ Sat, 08 Sep 2018 13:17:54: 3000000 INFO @ Sat, 08 Sep 2018 13:17:54: 3000000 INFO @ Sat, 08 Sep 2018 13:17:54: 3000000 INFO @ Sat, 08 Sep 2018 13:18:00: 4000000 INFO @ Sat, 08 Sep 2018 13:18:00: 4000000 INFO @ Sat, 08 Sep 2018 13:18:00: 4000000 INFO @ Sat, 08 Sep 2018 13:18:07: 5000000 INFO @ Sat, 08 Sep 2018 13:18:07: 5000000 INFO @ Sat, 08 Sep 2018 13:18:07: 5000000 INFO @ Sat, 08 Sep 2018 13:18:13: 6000000 INFO @ Sat, 08 Sep 2018 13:18:13: 6000000 INFO @ Sat, 08 Sep 2018 13:18:13: 6000000 INFO @ Sat, 08 Sep 2018 13:18:19: 7000000 INFO @ Sat, 08 Sep 2018 13:18:19: 7000000 INFO @ Sat, 08 Sep 2018 13:18:20: 7000000 INFO @ Sat, 08 Sep 2018 13:18:25: 8000000 INFO @ Sat, 08 Sep 2018 13:18:25: 8000000 INFO @ Sat, 08 Sep 2018 13:18:26: 8000000 INFO @ Sat, 08 Sep 2018 13:18:32: 9000000 INFO @ Sat, 08 Sep 2018 13:18:32: 9000000 INFO @ Sat, 08 Sep 2018 13:18:32: 9000000 INFO @ Sat, 08 Sep 2018 13:18:38: 10000000 INFO @ Sat, 08 Sep 2018 13:18:38: 10000000 INFO @ Sat, 08 Sep 2018 13:18:39: 10000000 INFO @ Sat, 08 Sep 2018 13:18:44: 11000000 INFO @ Sat, 08 Sep 2018 13:18:44: 11000000 INFO @ Sat, 08 Sep 2018 13:18:45: 11000000 INFO @ Sat, 08 Sep 2018 13:18:51: 12000000 INFO @ Sat, 08 Sep 2018 13:18:51: 12000000 INFO @ Sat, 08 Sep 2018 13:18:51: 12000000 INFO @ Sat, 08 Sep 2018 13:18:57: 13000000 INFO @ Sat, 08 Sep 2018 13:18:57: 13000000 INFO @ Sat, 08 Sep 2018 13:18:58: 13000000 INFO @ Sat, 08 Sep 2018 13:19:03: 14000000 INFO @ Sat, 08 Sep 2018 13:19:03: 14000000 INFO @ Sat, 08 Sep 2018 13:19:04: 14000000 INFO @ Sat, 08 Sep 2018 13:19:09: 15000000 INFO @ Sat, 08 Sep 2018 13:19:09: 15000000 INFO @ Sat, 08 Sep 2018 13:19:10: 15000000 INFO @ Sat, 08 Sep 2018 13:19:16: 16000000 INFO @ Sat, 08 Sep 2018 13:19:16: 16000000 INFO @ Sat, 08 Sep 2018 13:19:17: 16000000 INFO @ Sat, 08 Sep 2018 13:19:22: 17000000 INFO @ Sat, 08 Sep 2018 13:19:22: 17000000 INFO @ Sat, 08 Sep 2018 13:19:23: 17000000 INFO @ Sat, 08 Sep 2018 13:19:28: 18000000 INFO @ Sat, 08 Sep 2018 13:19:28: 18000000 INFO @ Sat, 08 Sep 2018 13:19:30: 18000000 INFO @ Sat, 08 Sep 2018 13:19:34: 19000000 INFO @ Sat, 08 Sep 2018 13:19:34: 19000000 INFO @ Sat, 08 Sep 2018 13:19:36: 19000000 INFO @ Sat, 08 Sep 2018 13:19:41: 20000000 INFO @ Sat, 08 Sep 2018 13:19:41: 20000000 INFO @ Sat, 08 Sep 2018 13:19:42: 20000000 INFO @ Sat, 08 Sep 2018 13:19:47: 21000000 INFO @ Sat, 08 Sep 2018 13:19:47: 21000000 INFO @ Sat, 08 Sep 2018 13:19:49: 21000000 INFO @ Sat, 08 Sep 2018 13:19:53: 22000000 INFO @ Sat, 08 Sep 2018 13:19:53: 22000000 INFO @ Sat, 08 Sep 2018 13:19:55: 22000000 INFO @ Sat, 08 Sep 2018 13:20:00: 23000000 INFO @ Sat, 08 Sep 2018 13:20:00: 23000000 INFO @ Sat, 08 Sep 2018 13:20:01: 23000000 INFO @ Sat, 08 Sep 2018 13:20:06: 24000000 INFO @ Sat, 08 Sep 2018 13:20:06: 24000000 INFO @ Sat, 08 Sep 2018 13:20:08: 24000000 INFO @ Sat, 08 Sep 2018 13:20:12: 25000000 INFO @ Sat, 08 Sep 2018 13:20:12: 25000000 INFO @ Sat, 08 Sep 2018 13:20:14: 25000000 INFO @ Sat, 08 Sep 2018 13:20:19: 26000000 INFO @ Sat, 08 Sep 2018 13:20:19: 26000000 INFO @ Sat, 08 Sep 2018 13:20:20: 26000000 INFO @ Sat, 08 Sep 2018 13:20:25: 27000000 INFO @ Sat, 08 Sep 2018 13:20:25: 27000000 INFO @ Sat, 08 Sep 2018 13:20:27: 27000000 INFO @ Sat, 08 Sep 2018 13:20:31: 28000000 INFO @ Sat, 08 Sep 2018 13:20:31: 28000000 INFO @ Sat, 08 Sep 2018 13:20:33: 28000000 INFO @ Sat, 08 Sep 2018 13:20:37: 29000000 INFO @ Sat, 08 Sep 2018 13:20:37: 29000000 INFO @ Sat, 08 Sep 2018 13:20:39: 29000000 INFO @ Sat, 08 Sep 2018 13:20:41: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:20:41: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:20:41: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:20:41: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:20:41: #1 total tags in treatment: 29539000 INFO @ Sat, 08 Sep 2018 13:20:41: #1 total tags in treatment: 29539000 INFO @ Sat, 08 Sep 2018 13:20:41: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:20:41: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:20:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:20:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:20:42: #1 tags after filtering in treatment: 29539000 INFO @ Sat, 08 Sep 2018 13:20:42: #1 tags after filtering in treatment: 29539000 INFO @ Sat, 08 Sep 2018 13:20:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:20:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:20:42: #1 finished! INFO @ Sat, 08 Sep 2018 13:20:42: #1 finished! INFO @ Sat, 08 Sep 2018 13:20:42: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:20:42: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:20:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:20:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:20:43: #1 tag size is determined as 50 bps INFO @ Sat, 08 Sep 2018 13:20:43: #1 tag size = 50 INFO @ Sat, 08 Sep 2018 13:20:43: #1 total tags in treatment: 29539000 INFO @ Sat, 08 Sep 2018 13:20:43: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:20:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:20:44: #2 number of paired peaks: 16 WARNING @ Sat, 08 Sep 2018 13:20:44: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:20:44: Process for pairing-model is terminated! INFO @ Sat, 08 Sep 2018 13:20:44: #1 tags after filtering in treatment: 29539000 INFO @ Sat, 08 Sep 2018 13:20:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:20:44: #1 finished! INFO @ Sat, 08 Sep 2018 13:20:44: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:20:44: #2 looking for paired plus/minus strand peaks... cat: SRX2618505.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618505.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618505.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618505.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:20:44: #2 number of paired peaks: 16 WARNING @ Sat, 08 Sep 2018 13:20:44: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:20:44: Process for pairing-model is terminated! cat: SRX2618505.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618505.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618505.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618505.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:20:46: #2 number of paired peaks: 16 WARNING @ Sat, 08 Sep 2018 13:20:46: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:20:46: Process for pairing-model is terminated! cat: SRX2618505.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2618505.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618505.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2618505.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。