Job ID = 1294362 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-06-02T21:59:08 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 28,196,192 reads read : 56,392,384 reads written : 56,392,384 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:16:39 28196192 reads; of these: 28196192 (100.00%) were paired; of these: 1531561 (5.43%) aligned concordantly 0 times 19123722 (67.82%) aligned concordantly exactly 1 time 7540909 (26.74%) aligned concordantly >1 times ---- 1531561 pairs aligned concordantly 0 times; of these: 179163 (11.70%) aligned discordantly 1 time ---- 1352398 pairs aligned 0 times concordantly or discordantly; of these: 2704796 mates make up the pairs; of these: 1583676 (58.55%) aligned 0 times 744338 (27.52%) aligned exactly 1 time 376782 (13.93%) aligned >1 times 97.19% overall alignment rate Time searching: 01:16:39 Overall time: 01:16:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4273644 / 26812782 = 0.1594 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 08:51:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 08:51:18: #1 read tag files... INFO @ Mon, 03 Jun 2019 08:51:18: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 08:51:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 08:51:18: #1 read tag files... INFO @ Mon, 03 Jun 2019 08:51:18: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 08:51:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 08:51:18: #1 read tag files... INFO @ Mon, 03 Jun 2019 08:51:18: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 08:51:26: 1000000 INFO @ Mon, 03 Jun 2019 08:51:26: 1000000 INFO @ Mon, 03 Jun 2019 08:51:28: 1000000 INFO @ Mon, 03 Jun 2019 08:51:34: 2000000 INFO @ Mon, 03 Jun 2019 08:51:35: 2000000 INFO @ Mon, 03 Jun 2019 08:51:37: 2000000 INFO @ Mon, 03 Jun 2019 08:51:42: 3000000 INFO @ Mon, 03 Jun 2019 08:51:43: 3000000 INFO @ Mon, 03 Jun 2019 08:51:47: 3000000 INFO @ Mon, 03 Jun 2019 08:51:50: 4000000 INFO @ Mon, 03 Jun 2019 08:51:51: 4000000 INFO @ Mon, 03 Jun 2019 08:51:56: 4000000 INFO @ Mon, 03 Jun 2019 08:51:58: 5000000 INFO @ Mon, 03 Jun 2019 08:51:59: 5000000 INFO @ Mon, 03 Jun 2019 08:52:05: 5000000 INFO @ Mon, 03 Jun 2019 08:52:06: 6000000 INFO @ Mon, 03 Jun 2019 08:52:07: 6000000 INFO @ Mon, 03 Jun 2019 08:52:14: 7000000 INFO @ Mon, 03 Jun 2019 08:52:15: 7000000 INFO @ Mon, 03 Jun 2019 08:52:15: 6000000 INFO @ Mon, 03 Jun 2019 08:52:22: 8000000 INFO @ Mon, 03 Jun 2019 08:52:23: 8000000 INFO @ Mon, 03 Jun 2019 08:52:25: 7000000 INFO @ Mon, 03 Jun 2019 08:52:30: 9000000 INFO @ Mon, 03 Jun 2019 08:52:30: 9000000 INFO @ Mon, 03 Jun 2019 08:52:34: 8000000 INFO @ Mon, 03 Jun 2019 08:52:37: 10000000 INFO @ Mon, 03 Jun 2019 08:52:38: 10000000 INFO @ Mon, 03 Jun 2019 08:52:44: 9000000 INFO @ Mon, 03 Jun 2019 08:52:45: 11000000 INFO @ Mon, 03 Jun 2019 08:52:46: 11000000 INFO @ Mon, 03 Jun 2019 08:52:52: 12000000 INFO @ Mon, 03 Jun 2019 08:52:53: 12000000 INFO @ Mon, 03 Jun 2019 08:52:53: 10000000 INFO @ Mon, 03 Jun 2019 08:53:00: 13000000 INFO @ Mon, 03 Jun 2019 08:53:01: 13000000 INFO @ Mon, 03 Jun 2019 08:53:03: 11000000 INFO @ Mon, 03 Jun 2019 08:53:07: 14000000 INFO @ Mon, 03 Jun 2019 08:53:08: 14000000 INFO @ Mon, 03 Jun 2019 08:53:12: 12000000 INFO @ Mon, 03 Jun 2019 08:53:14: 15000000 INFO @ Mon, 03 Jun 2019 08:53:16: 15000000 INFO @ Mon, 03 Jun 2019 08:53:22: 13000000 INFO @ Mon, 03 Jun 2019 08:53:22: 16000000 INFO @ Mon, 03 Jun 2019 08:53:24: 16000000 INFO @ Mon, 03 Jun 2019 08:53:29: 17000000 INFO @ Mon, 03 Jun 2019 08:53:31: 14000000 INFO @ Mon, 03 Jun 2019 08:53:31: 17000000 INFO @ Mon, 03 Jun 2019 08:53:37: 18000000 INFO @ Mon, 03 Jun 2019 08:53:39: 18000000 INFO @ Mon, 03 Jun 2019 08:53:40: 15000000 INFO @ Mon, 03 Jun 2019 08:53:44: 19000000 INFO @ Mon, 03 Jun 2019 08:53:47: 19000000 INFO @ Mon, 03 Jun 2019 08:53:49: 16000000 INFO @ Mon, 03 Jun 2019 08:53:52: 20000000 INFO @ Mon, 03 Jun 2019 08:53:54: 20000000 INFO @ Mon, 03 Jun 2019 08:53:59: 17000000 INFO @ Mon, 03 Jun 2019 08:53:59: 21000000 INFO @ Mon, 03 Jun 2019 08:54:02: 21000000 INFO @ Mon, 03 Jun 2019 08:54:07: 22000000 INFO @ Mon, 03 Jun 2019 08:54:08: 18000000 INFO @ Mon, 03 Jun 2019 08:54:10: 22000000 INFO @ Mon, 03 Jun 2019 08:54:14: 23000000 INFO @ Mon, 03 Jun 2019 08:54:17: 23000000 INFO @ Mon, 03 Jun 2019 08:54:18: 19000000 INFO @ Mon, 03 Jun 2019 08:54:22: 24000000 INFO @ Mon, 03 Jun 2019 08:54:25: 24000000 INFO @ Mon, 03 Jun 2019 08:54:28: 20000000 INFO @ Mon, 03 Jun 2019 08:54:29: 25000000 INFO @ Mon, 03 Jun 2019 08:54:33: 25000000 INFO @ Mon, 03 Jun 2019 08:54:36: 26000000 INFO @ Mon, 03 Jun 2019 08:54:37: 21000000 INFO @ Mon, 03 Jun 2019 08:54:40: 26000000 INFO @ Mon, 03 Jun 2019 08:54:43: 27000000 INFO @ Mon, 03 Jun 2019 08:54:47: 22000000 INFO @ Mon, 03 Jun 2019 08:54:48: 27000000 INFO @ Mon, 03 Jun 2019 08:54:50: 28000000 INFO @ Mon, 03 Jun 2019 08:54:55: 28000000 INFO @ Mon, 03 Jun 2019 08:54:56: 23000000 INFO @ Mon, 03 Jun 2019 08:54:57: 29000000 INFO @ Mon, 03 Jun 2019 08:55:03: 29000000 INFO @ Mon, 03 Jun 2019 08:55:04: 30000000 INFO @ Mon, 03 Jun 2019 08:55:06: 24000000 INFO @ Mon, 03 Jun 2019 08:55:10: 30000000 INFO @ Mon, 03 Jun 2019 08:55:12: 31000000 INFO @ Mon, 03 Jun 2019 08:55:15: 25000000 INFO @ Mon, 03 Jun 2019 08:55:17: 31000000 INFO @ Mon, 03 Jun 2019 08:55:19: 32000000 INFO @ Mon, 03 Jun 2019 08:55:24: 26000000 INFO @ Mon, 03 Jun 2019 08:55:25: 32000000 INFO @ Mon, 03 Jun 2019 08:55:26: 33000000 INFO @ Mon, 03 Jun 2019 08:55:32: 33000000 INFO @ Mon, 03 Jun 2019 08:55:33: 34000000 INFO @ Mon, 03 Jun 2019 08:55:33: 27000000 INFO @ Mon, 03 Jun 2019 08:55:39: 34000000 INFO @ Mon, 03 Jun 2019 08:55:41: 35000000 INFO @ Mon, 03 Jun 2019 08:55:42: 28000000 INFO @ Mon, 03 Jun 2019 08:55:47: 35000000 INFO @ Mon, 03 Jun 2019 08:55:49: 36000000 INFO @ Mon, 03 Jun 2019 08:55:51: 29000000 INFO @ Mon, 03 Jun 2019 08:55:56: 36000000 INFO @ Mon, 03 Jun 2019 08:55:58: 37000000 INFO @ Mon, 03 Jun 2019 08:55:59: 30000000 INFO @ Mon, 03 Jun 2019 08:56:05: 37000000 INFO @ Mon, 03 Jun 2019 08:56:05: 38000000 INFO @ Mon, 03 Jun 2019 08:56:08: 31000000 INFO @ Mon, 03 Jun 2019 08:56:12: 38000000 INFO @ Mon, 03 Jun 2019 08:56:12: 39000000 INFO @ Mon, 03 Jun 2019 08:56:17: 32000000 INFO @ Mon, 03 Jun 2019 08:56:19: 39000000 INFO @ Mon, 03 Jun 2019 08:56:20: 40000000 INFO @ Mon, 03 Jun 2019 08:56:26: 33000000 INFO @ Mon, 03 Jun 2019 08:56:27: 41000000 INFO @ Mon, 03 Jun 2019 08:56:27: 40000000 INFO @ Mon, 03 Jun 2019 08:56:34: 42000000 INFO @ Mon, 03 Jun 2019 08:56:34: 34000000 INFO @ Mon, 03 Jun 2019 08:56:35: 41000000 INFO @ Mon, 03 Jun 2019 08:56:41: 43000000 INFO @ Mon, 03 Jun 2019 08:56:42: 42000000 INFO @ Mon, 03 Jun 2019 08:56:43: 35000000 INFO @ Mon, 03 Jun 2019 08:56:48: 44000000 INFO @ Mon, 03 Jun 2019 08:56:50: 43000000 INFO @ Mon, 03 Jun 2019 08:56:52: 36000000 INFO @ Mon, 03 Jun 2019 08:56:55: 45000000 INFO @ Mon, 03 Jun 2019 08:56:57: 44000000 INFO @ Mon, 03 Jun 2019 08:57:00: 37000000 INFO @ Mon, 03 Jun 2019 08:57:02: 46000000 INFO @ Mon, 03 Jun 2019 08:57:05: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 08:57:05: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 08:57:05: #1 total tags in treatment: 22399859 INFO @ Mon, 03 Jun 2019 08:57:05: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 08:57:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 08:57:05: 45000000 INFO @ Mon, 03 Jun 2019 08:57:05: #1 tags after filtering in treatment: 20071703 INFO @ Mon, 03 Jun 2019 08:57:05: #1 Redundant rate of treatment: 0.10 INFO @ Mon, 03 Jun 2019 08:57:05: #1 finished! INFO @ Mon, 03 Jun 2019 08:57:05: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 08:57:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 08:57:07: #2 number of paired peaks: 466 WARNING @ Mon, 03 Jun 2019 08:57:07: Fewer paired peaks (466) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 466 pairs to build model! INFO @ Mon, 03 Jun 2019 08:57:07: start model_add_line... INFO @ Mon, 03 Jun 2019 08:57:07: start X-correlation... INFO @ Mon, 03 Jun 2019 08:57:07: end of X-cor INFO @ Mon, 03 Jun 2019 08:57:07: #2 finished! INFO @ Mon, 03 Jun 2019 08:57:07: #2 predicted fragment length is 142 bps INFO @ Mon, 03 Jun 2019 08:57:07: #2 alternative fragment length(s) may be 4,142 bps INFO @ Mon, 03 Jun 2019 08:57:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.10_model.r INFO @ Mon, 03 Jun 2019 08:57:07: #3 Call peaks... INFO @ Mon, 03 Jun 2019 08:57:07: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 08:57:09: 38000000 INFO @ Mon, 03 Jun 2019 08:57:12: 46000000 INFO @ Mon, 03 Jun 2019 08:57:14: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 08:57:14: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 08:57:14: #1 total tags in treatment: 22399859 INFO @ Mon, 03 Jun 2019 08:57:14: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 08:57:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 08:57:15: #1 tags after filtering in treatment: 20071703 INFO @ Mon, 03 Jun 2019 08:57:15: #1 Redundant rate of treatment: 0.10 INFO @ Mon, 03 Jun 2019 08:57:15: #1 finished! INFO @ Mon, 03 Jun 2019 08:57:15: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 08:57:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 08:57:16: #2 number of paired peaks: 466 WARNING @ Mon, 03 Jun 2019 08:57:16: Fewer paired peaks (466) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 466 pairs to build model! INFO @ Mon, 03 Jun 2019 08:57:16: start model_add_line... INFO @ Mon, 03 Jun 2019 08:57:17: start X-correlation... INFO @ Mon, 03 Jun 2019 08:57:17: end of X-cor INFO @ Mon, 03 Jun 2019 08:57:17: #2 finished! INFO @ Mon, 03 Jun 2019 08:57:17: #2 predicted fragment length is 142 bps INFO @ Mon, 03 Jun 2019 08:57:17: #2 alternative fragment length(s) may be 4,142 bps INFO @ Mon, 03 Jun 2019 08:57:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.05_model.r INFO @ Mon, 03 Jun 2019 08:57:17: #3 Call peaks... INFO @ Mon, 03 Jun 2019 08:57:17: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 08:57:17: 39000000 INFO @ Mon, 03 Jun 2019 08:57:27: 40000000 INFO @ Mon, 03 Jun 2019 08:57:35: 41000000 INFO @ Mon, 03 Jun 2019 08:57:43: 42000000 INFO @ Mon, 03 Jun 2019 08:57:51: 43000000 INFO @ Mon, 03 Jun 2019 08:57:59: 44000000 INFO @ Mon, 03 Jun 2019 08:58:04: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 08:58:07: 45000000 INFO @ Mon, 03 Jun 2019 08:58:13: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 08:58:15: 46000000 INFO @ Mon, 03 Jun 2019 08:58:17: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 08:58:17: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 08:58:17: #1 total tags in treatment: 22399859 INFO @ Mon, 03 Jun 2019 08:58:17: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 08:58:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 08:58:18: #1 tags after filtering in treatment: 20071703 INFO @ Mon, 03 Jun 2019 08:58:18: #1 Redundant rate of treatment: 0.10 INFO @ Mon, 03 Jun 2019 08:58:18: #1 finished! INFO @ Mon, 03 Jun 2019 08:58:18: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 08:58:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 08:58:20: #2 number of paired peaks: 466 WARNING @ Mon, 03 Jun 2019 08:58:20: Fewer paired peaks (466) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 466 pairs to build model! INFO @ Mon, 03 Jun 2019 08:58:20: start model_add_line... INFO @ Mon, 03 Jun 2019 08:58:20: start X-correlation... INFO @ Mon, 03 Jun 2019 08:58:20: end of X-cor INFO @ Mon, 03 Jun 2019 08:58:20: #2 finished! INFO @ Mon, 03 Jun 2019 08:58:20: #2 predicted fragment length is 142 bps INFO @ Mon, 03 Jun 2019 08:58:20: #2 alternative fragment length(s) may be 4,142 bps INFO @ Mon, 03 Jun 2019 08:58:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.20_model.r INFO @ Mon, 03 Jun 2019 08:58:20: #3 Call peaks... INFO @ Mon, 03 Jun 2019 08:58:20: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 08:58:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.10_peaks.xls INFO @ Mon, 03 Jun 2019 08:58:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 08:58:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.10_summits.bed INFO @ Mon, 03 Jun 2019 08:58:30: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (1929 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 08:58:39: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.05_peaks.xls INFO @ Mon, 03 Jun 2019 08:58:39: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 08:58:39: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.05_summits.bed INFO @ Mon, 03 Jun 2019 08:58:39: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (3144 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 08:59:17: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 08:59:44: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.20_peaks.xls INFO @ Mon, 03 Jun 2019 08:59:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 08:59:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612527/SRX2612527.20_summits.bed INFO @ Mon, 03 Jun 2019 08:59:44: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (1111 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。