Job ID = 1294357 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-06-02T21:56:16 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:04:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:04:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:04:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:05:46 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 29,606,781 reads read : 59,213,562 reads written : 59,213,562 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:59:24 29606781 reads; of these: 29606781 (100.00%) were paired; of these: 2468698 (8.34%) aligned concordantly 0 times 22025714 (74.39%) aligned concordantly exactly 1 time 5112369 (17.27%) aligned concordantly >1 times ---- 2468698 pairs aligned concordantly 0 times; of these: 402757 (16.31%) aligned discordantly 1 time ---- 2065941 pairs aligned 0 times concordantly or discordantly; of these: 4131882 mates make up the pairs; of these: 2760432 (66.81%) aligned 0 times 749336 (18.14%) aligned exactly 1 time 622114 (15.06%) aligned >1 times 95.34% overall alignment rate Time searching: 00:59:24 Overall time: 00:59:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 5106040 / 27524013 = 0.1855 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 08:32:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 08:32:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 08:32:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 08:32:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 08:32:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 08:32:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 08:32:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 08:32:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 08:32:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 08:32:23: 1000000 INFO @ Mon, 03 Jun 2019 08:32:23: 1000000 INFO @ Mon, 03 Jun 2019 08:32:23: 1000000 INFO @ Mon, 03 Jun 2019 08:32:30: 2000000 INFO @ Mon, 03 Jun 2019 08:32:31: 2000000 INFO @ Mon, 03 Jun 2019 08:32:31: 2000000 INFO @ Mon, 03 Jun 2019 08:32:38: 3000000 INFO @ Mon, 03 Jun 2019 08:32:38: 3000000 INFO @ Mon, 03 Jun 2019 08:32:38: 3000000 INFO @ Mon, 03 Jun 2019 08:32:46: 4000000 INFO @ Mon, 03 Jun 2019 08:32:46: 4000000 INFO @ Mon, 03 Jun 2019 08:32:47: 4000000 INFO @ Mon, 03 Jun 2019 08:32:54: 5000000 INFO @ Mon, 03 Jun 2019 08:32:54: 5000000 INFO @ Mon, 03 Jun 2019 08:32:55: 5000000 INFO @ Mon, 03 Jun 2019 08:33:02: 6000000 INFO @ Mon, 03 Jun 2019 08:33:02: 6000000 INFO @ Mon, 03 Jun 2019 08:33:03: 6000000 INFO @ Mon, 03 Jun 2019 08:33:10: 7000000 INFO @ Mon, 03 Jun 2019 08:33:10: 7000000 INFO @ Mon, 03 Jun 2019 08:33:11: 7000000 INFO @ Mon, 03 Jun 2019 08:33:18: 8000000 INFO @ Mon, 03 Jun 2019 08:33:18: 8000000 INFO @ Mon, 03 Jun 2019 08:33:19: 8000000 INFO @ Mon, 03 Jun 2019 08:33:25: 9000000 INFO @ Mon, 03 Jun 2019 08:33:25: 9000000 INFO @ Mon, 03 Jun 2019 08:33:26: 9000000 INFO @ Mon, 03 Jun 2019 08:33:34: 10000000 INFO @ Mon, 03 Jun 2019 08:33:34: 10000000 INFO @ Mon, 03 Jun 2019 08:33:34: 10000000 INFO @ Mon, 03 Jun 2019 08:33:41: 11000000 INFO @ Mon, 03 Jun 2019 08:33:41: 11000000 INFO @ Mon, 03 Jun 2019 08:33:42: 11000000 INFO @ Mon, 03 Jun 2019 08:33:49: 12000000 INFO @ Mon, 03 Jun 2019 08:33:49: 12000000 INFO @ Mon, 03 Jun 2019 08:33:49: 12000000 INFO @ Mon, 03 Jun 2019 08:33:56: 13000000 INFO @ Mon, 03 Jun 2019 08:33:56: 13000000 INFO @ Mon, 03 Jun 2019 08:33:57: 13000000 INFO @ Mon, 03 Jun 2019 08:34:02: 14000000 INFO @ Mon, 03 Jun 2019 08:34:03: 14000000 INFO @ Mon, 03 Jun 2019 08:34:04: 14000000 INFO @ Mon, 03 Jun 2019 08:34:09: 15000000 INFO @ Mon, 03 Jun 2019 08:34:10: 15000000 INFO @ Mon, 03 Jun 2019 08:34:12: 15000000 INFO @ Mon, 03 Jun 2019 08:34:16: 16000000 INFO @ Mon, 03 Jun 2019 08:34:16: 16000000 INFO @ Mon, 03 Jun 2019 08:34:21: 16000000 INFO @ Mon, 03 Jun 2019 08:34:23: 17000000 INFO @ Mon, 03 Jun 2019 08:34:23: 17000000 INFO @ Mon, 03 Jun 2019 08:34:28: 17000000 INFO @ Mon, 03 Jun 2019 08:34:30: 18000000 INFO @ Mon, 03 Jun 2019 08:34:30: 18000000 INFO @ Mon, 03 Jun 2019 08:34:36: 18000000 INFO @ Mon, 03 Jun 2019 08:34:38: 19000000 INFO @ Mon, 03 Jun 2019 08:34:38: 19000000 INFO @ Mon, 03 Jun 2019 08:34:44: 19000000 INFO @ Mon, 03 Jun 2019 08:34:45: 20000000 INFO @ Mon, 03 Jun 2019 08:34:45: 20000000 INFO @ Mon, 03 Jun 2019 08:34:51: 20000000 INFO @ Mon, 03 Jun 2019 08:34:52: 21000000 INFO @ Mon, 03 Jun 2019 08:34:52: 21000000 INFO @ Mon, 03 Jun 2019 08:34:58: 21000000 INFO @ Mon, 03 Jun 2019 08:34:59: 22000000 INFO @ Mon, 03 Jun 2019 08:34:59: 22000000 INFO @ Mon, 03 Jun 2019 08:35:05: 22000000 INFO @ Mon, 03 Jun 2019 08:35:05: 23000000 INFO @ Mon, 03 Jun 2019 08:35:06: 23000000 INFO @ Mon, 03 Jun 2019 08:35:12: 23000000 INFO @ Mon, 03 Jun 2019 08:35:12: 24000000 INFO @ Mon, 03 Jun 2019 08:35:12: 24000000 INFO @ Mon, 03 Jun 2019 08:35:19: 24000000 INFO @ Mon, 03 Jun 2019 08:35:19: 25000000 INFO @ Mon, 03 Jun 2019 08:35:19: 25000000 INFO @ Mon, 03 Jun 2019 08:35:26: 26000000 INFO @ Mon, 03 Jun 2019 08:35:26: 25000000 INFO @ Mon, 03 Jun 2019 08:35:26: 26000000 INFO @ Mon, 03 Jun 2019 08:35:33: 27000000 INFO @ Mon, 03 Jun 2019 08:35:33: 27000000 INFO @ Mon, 03 Jun 2019 08:35:33: 26000000 INFO @ Mon, 03 Jun 2019 08:35:40: 28000000 INFO @ Mon, 03 Jun 2019 08:35:40: 28000000 INFO @ Mon, 03 Jun 2019 08:35:41: 27000000 INFO @ Mon, 03 Jun 2019 08:35:46: 29000000 INFO @ Mon, 03 Jun 2019 08:35:46: 29000000 INFO @ Mon, 03 Jun 2019 08:35:49: 28000000 INFO @ Mon, 03 Jun 2019 08:35:53: 30000000 INFO @ Mon, 03 Jun 2019 08:35:53: 30000000 INFO @ Mon, 03 Jun 2019 08:35:56: 29000000 INFO @ Mon, 03 Jun 2019 08:36:00: 31000000 INFO @ Mon, 03 Jun 2019 08:36:00: 31000000 INFO @ Mon, 03 Jun 2019 08:36:04: 30000000 INFO @ Mon, 03 Jun 2019 08:36:06: 32000000 INFO @ Mon, 03 Jun 2019 08:36:08: 32000000 INFO @ Mon, 03 Jun 2019 08:36:11: 31000000 INFO @ Mon, 03 Jun 2019 08:36:12: 33000000 INFO @ Mon, 03 Jun 2019 08:36:14: 33000000 INFO @ Mon, 03 Jun 2019 08:36:18: 32000000 INFO @ Mon, 03 Jun 2019 08:36:19: 34000000 INFO @ Mon, 03 Jun 2019 08:36:20: 34000000 INFO @ Mon, 03 Jun 2019 08:36:25: 35000000 INFO @ Mon, 03 Jun 2019 08:36:25: 33000000 INFO @ Mon, 03 Jun 2019 08:36:27: 35000000 INFO @ Mon, 03 Jun 2019 08:36:31: 36000000 INFO @ Mon, 03 Jun 2019 08:36:33: 34000000 INFO @ Mon, 03 Jun 2019 08:36:33: 36000000 INFO @ Mon, 03 Jun 2019 08:36:37: 37000000 INFO @ Mon, 03 Jun 2019 08:36:39: 37000000 INFO @ Mon, 03 Jun 2019 08:36:40: 35000000 INFO @ Mon, 03 Jun 2019 08:36:43: 38000000 INFO @ Mon, 03 Jun 2019 08:36:46: 38000000 INFO @ Mon, 03 Jun 2019 08:36:47: 36000000 INFO @ Mon, 03 Jun 2019 08:36:49: 39000000 INFO @ Mon, 03 Jun 2019 08:36:52: 39000000 INFO @ Mon, 03 Jun 2019 08:36:54: 37000000 INFO @ Mon, 03 Jun 2019 08:36:55: 40000000 INFO @ Mon, 03 Jun 2019 08:36:58: 40000000 INFO @ Mon, 03 Jun 2019 08:37:01: 38000000 INFO @ Mon, 03 Jun 2019 08:37:01: 41000000 INFO @ Mon, 03 Jun 2019 08:37:05: 41000000 INFO @ Mon, 03 Jun 2019 08:37:07: 42000000 INFO @ Mon, 03 Jun 2019 08:37:09: 39000000 INFO @ Mon, 03 Jun 2019 08:37:11: 42000000 INFO @ Mon, 03 Jun 2019 08:37:13: 43000000 INFO @ Mon, 03 Jun 2019 08:37:16: 40000000 INFO @ Mon, 03 Jun 2019 08:37:17: 43000000 INFO @ Mon, 03 Jun 2019 08:37:19: 44000000 INFO @ Mon, 03 Jun 2019 08:37:24: 41000000 INFO @ Mon, 03 Jun 2019 08:37:24: 44000000 INFO @ Mon, 03 Jun 2019 08:37:25: 45000000 INFO @ Mon, 03 Jun 2019 08:37:31: 42000000 INFO @ Mon, 03 Jun 2019 08:37:31: 45000000 INFO @ Mon, 03 Jun 2019 08:37:32: 46000000 INFO @ Mon, 03 Jun 2019 08:37:34: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 08:37:34: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 08:37:34: #1 total tags in treatment: 22050112 INFO @ Mon, 03 Jun 2019 08:37:34: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 08:37:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 08:37:34: #1 tags after filtering in treatment: 15464374 INFO @ Mon, 03 Jun 2019 08:37:34: #1 Redundant rate of treatment: 0.30 INFO @ Mon, 03 Jun 2019 08:37:34: #1 finished! INFO @ Mon, 03 Jun 2019 08:37:34: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 08:37:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 08:37:36: #2 number of paired peaks: 5864 INFO @ Mon, 03 Jun 2019 08:37:36: start model_add_line... INFO @ Mon, 03 Jun 2019 08:37:36: start X-correlation... INFO @ Mon, 03 Jun 2019 08:37:36: end of X-cor INFO @ Mon, 03 Jun 2019 08:37:36: #2 finished! INFO @ Mon, 03 Jun 2019 08:37:36: #2 predicted fragment length is 222 bps INFO @ Mon, 03 Jun 2019 08:37:36: #2 alternative fragment length(s) may be 222 bps INFO @ Mon, 03 Jun 2019 08:37:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.10_model.r INFO @ Mon, 03 Jun 2019 08:37:36: #3 Call peaks... INFO @ Mon, 03 Jun 2019 08:37:36: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 08:37:38: 43000000 INFO @ Mon, 03 Jun 2019 08:37:38: 46000000 INFO @ Mon, 03 Jun 2019 08:37:40: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 08:37:40: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 08:37:40: #1 total tags in treatment: 22050112 INFO @ Mon, 03 Jun 2019 08:37:40: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 08:37:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 08:37:40: #1 tags after filtering in treatment: 15464374 INFO @ Mon, 03 Jun 2019 08:37:40: #1 Redundant rate of treatment: 0.30 INFO @ Mon, 03 Jun 2019 08:37:40: #1 finished! INFO @ Mon, 03 Jun 2019 08:37:40: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 08:37:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 08:37:42: #2 number of paired peaks: 5864 INFO @ Mon, 03 Jun 2019 08:37:42: start model_add_line... INFO @ Mon, 03 Jun 2019 08:37:42: start X-correlation... INFO @ Mon, 03 Jun 2019 08:37:42: end of X-cor INFO @ Mon, 03 Jun 2019 08:37:42: #2 finished! INFO @ Mon, 03 Jun 2019 08:37:42: #2 predicted fragment length is 222 bps INFO @ Mon, 03 Jun 2019 08:37:42: #2 alternative fragment length(s) may be 222 bps INFO @ Mon, 03 Jun 2019 08:37:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.05_model.r INFO @ Mon, 03 Jun 2019 08:37:42: #3 Call peaks... INFO @ Mon, 03 Jun 2019 08:37:42: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 08:37:44: 44000000 INFO @ Mon, 03 Jun 2019 08:37:51: 45000000 INFO @ Mon, 03 Jun 2019 08:37:58: 46000000 INFO @ Mon, 03 Jun 2019 08:38:00: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 08:38:00: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 08:38:00: #1 total tags in treatment: 22050112 INFO @ Mon, 03 Jun 2019 08:38:00: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 08:38:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 08:38:00: #1 tags after filtering in treatment: 15464374 INFO @ Mon, 03 Jun 2019 08:38:00: #1 Redundant rate of treatment: 0.30 INFO @ Mon, 03 Jun 2019 08:38:00: #1 finished! INFO @ Mon, 03 Jun 2019 08:38:00: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 08:38:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 08:38:02: #2 number of paired peaks: 5864 INFO @ Mon, 03 Jun 2019 08:38:02: start model_add_line... INFO @ Mon, 03 Jun 2019 08:38:03: start X-correlation... INFO @ Mon, 03 Jun 2019 08:38:03: end of X-cor INFO @ Mon, 03 Jun 2019 08:38:03: #2 finished! INFO @ Mon, 03 Jun 2019 08:38:03: #2 predicted fragment length is 222 bps INFO @ Mon, 03 Jun 2019 08:38:03: #2 alternative fragment length(s) may be 222 bps INFO @ Mon, 03 Jun 2019 08:38:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.20_model.r INFO @ Mon, 03 Jun 2019 08:38:03: #3 Call peaks... INFO @ Mon, 03 Jun 2019 08:38:03: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 08:38:37: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 08:38:44: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 08:39:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.10_peaks.xls INFO @ Mon, 03 Jun 2019 08:39:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 08:39:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.10_summits.bed INFO @ Mon, 03 Jun 2019 08:39:00: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (7383 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 08:39:04: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 08:39:07: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.05_peaks.xls INFO @ Mon, 03 Jun 2019 08:39:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 08:39:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.05_summits.bed INFO @ Mon, 03 Jun 2019 08:39:07: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (9196 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 08:39:27: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.20_peaks.xls INFO @ Mon, 03 Jun 2019 08:39:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 08:39:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612522/SRX2612522.20_summits.bed INFO @ Mon, 03 Jun 2019 08:39:27: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (5517 records, 4 fields): 9 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。