Job ID = 1294347 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-06-02T21:37:07 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T21:47:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T21:52:56 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T21:55:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-02T22:04:52 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 37,390,056 reads read : 74,780,112 reads written : 74,780,112 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:27:24 37390056 reads; of these: 37390056 (100.00%) were paired; of these: 2576743 (6.89%) aligned concordantly 0 times 26675561 (71.34%) aligned concordantly exactly 1 time 8137752 (21.76%) aligned concordantly >1 times ---- 2576743 pairs aligned concordantly 0 times; of these: 582923 (22.62%) aligned discordantly 1 time ---- 1993820 pairs aligned 0 times concordantly or discordantly; of these: 3987640 mates make up the pairs; of these: 2137624 (53.61%) aligned 0 times 1079942 (27.08%) aligned exactly 1 time 770074 (19.31%) aligned >1 times 97.14% overall alignment rate Time searching: 01:27:24 Overall time: 01:27:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1923718 / 35311426 = 0.0545 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 09:09:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 09:09:58: #1 read tag files... INFO @ Mon, 03 Jun 2019 09:09:58: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 09:09:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 09:09:58: #1 read tag files... INFO @ Mon, 03 Jun 2019 09:09:58: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 09:09:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 09:09:58: #1 read tag files... INFO @ Mon, 03 Jun 2019 09:09:58: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 09:10:08: 1000000 INFO @ Mon, 03 Jun 2019 09:10:12: 1000000 INFO @ Mon, 03 Jun 2019 09:10:12: 1000000 INFO @ Mon, 03 Jun 2019 09:10:19: 2000000 INFO @ Mon, 03 Jun 2019 09:10:25: 2000000 INFO @ Mon, 03 Jun 2019 09:10:26: 2000000 INFO @ Mon, 03 Jun 2019 09:10:29: 3000000 INFO @ Mon, 03 Jun 2019 09:10:39: 4000000 INFO @ Mon, 03 Jun 2019 09:10:39: 3000000 INFO @ Mon, 03 Jun 2019 09:10:40: 3000000 INFO @ Mon, 03 Jun 2019 09:10:49: 5000000 INFO @ Mon, 03 Jun 2019 09:10:53: 4000000 INFO @ Mon, 03 Jun 2019 09:10:54: 4000000 INFO @ Mon, 03 Jun 2019 09:11:00: 6000000 INFO @ Mon, 03 Jun 2019 09:11:07: 5000000 INFO @ Mon, 03 Jun 2019 09:11:08: 5000000 INFO @ Mon, 03 Jun 2019 09:11:11: 7000000 INFO @ Mon, 03 Jun 2019 09:11:20: 6000000 INFO @ Mon, 03 Jun 2019 09:11:21: 8000000 INFO @ Mon, 03 Jun 2019 09:11:21: 6000000 INFO @ Mon, 03 Jun 2019 09:11:31: 9000000 INFO @ Mon, 03 Jun 2019 09:11:34: 7000000 INFO @ Mon, 03 Jun 2019 09:11:35: 7000000 INFO @ Mon, 03 Jun 2019 09:11:41: 10000000 INFO @ Mon, 03 Jun 2019 09:11:47: 8000000 INFO @ Mon, 03 Jun 2019 09:11:48: 8000000 INFO @ Mon, 03 Jun 2019 09:11:51: 11000000 INFO @ Mon, 03 Jun 2019 09:12:00: 9000000 INFO @ Mon, 03 Jun 2019 09:12:01: 9000000 INFO @ Mon, 03 Jun 2019 09:12:01: 12000000 INFO @ Mon, 03 Jun 2019 09:12:11: 13000000 INFO @ Mon, 03 Jun 2019 09:12:14: 10000000 INFO @ Mon, 03 Jun 2019 09:12:14: 10000000 INFO @ Mon, 03 Jun 2019 09:12:21: 14000000 INFO @ Mon, 03 Jun 2019 09:12:26: 11000000 INFO @ Mon, 03 Jun 2019 09:12:27: 11000000 INFO @ Mon, 03 Jun 2019 09:12:32: 15000000 INFO @ Mon, 03 Jun 2019 09:12:38: 12000000 INFO @ Mon, 03 Jun 2019 09:12:39: 12000000 INFO @ Mon, 03 Jun 2019 09:12:43: 16000000 INFO @ Mon, 03 Jun 2019 09:12:51: 13000000 INFO @ Mon, 03 Jun 2019 09:12:52: 13000000 INFO @ Mon, 03 Jun 2019 09:12:53: 17000000 INFO @ Mon, 03 Jun 2019 09:13:04: 14000000 INFO @ Mon, 03 Jun 2019 09:13:04: 18000000 INFO @ Mon, 03 Jun 2019 09:13:05: 14000000 INFO @ Mon, 03 Jun 2019 09:13:15: 19000000 INFO @ Mon, 03 Jun 2019 09:13:17: 15000000 INFO @ Mon, 03 Jun 2019 09:13:18: 15000000 INFO @ Mon, 03 Jun 2019 09:13:26: 20000000 INFO @ Mon, 03 Jun 2019 09:13:30: 16000000 INFO @ Mon, 03 Jun 2019 09:13:31: 16000000 INFO @ Mon, 03 Jun 2019 09:13:36: 21000000 INFO @ Mon, 03 Jun 2019 09:13:42: 17000000 INFO @ Mon, 03 Jun 2019 09:13:44: 17000000 INFO @ Mon, 03 Jun 2019 09:13:46: 22000000 INFO @ Mon, 03 Jun 2019 09:13:55: 18000000 INFO @ Mon, 03 Jun 2019 09:13:56: 18000000 INFO @ Mon, 03 Jun 2019 09:13:56: 23000000 INFO @ Mon, 03 Jun 2019 09:14:07: 24000000 INFO @ Mon, 03 Jun 2019 09:14:07: 19000000 INFO @ Mon, 03 Jun 2019 09:14:09: 19000000 INFO @ Mon, 03 Jun 2019 09:14:17: 25000000 INFO @ Mon, 03 Jun 2019 09:14:20: 20000000 INFO @ Mon, 03 Jun 2019 09:14:22: 20000000 INFO @ Mon, 03 Jun 2019 09:14:27: 26000000 INFO @ Mon, 03 Jun 2019 09:14:33: 21000000 INFO @ Mon, 03 Jun 2019 09:14:35: 21000000 INFO @ Mon, 03 Jun 2019 09:14:37: 27000000 INFO @ Mon, 03 Jun 2019 09:14:45: 22000000 INFO @ Mon, 03 Jun 2019 09:14:47: 22000000 INFO @ Mon, 03 Jun 2019 09:14:47: 28000000 INFO @ Mon, 03 Jun 2019 09:14:58: 29000000 INFO @ Mon, 03 Jun 2019 09:14:58: 23000000 INFO @ Mon, 03 Jun 2019 09:15:00: 23000000 INFO @ Mon, 03 Jun 2019 09:15:08: 30000000 INFO @ Mon, 03 Jun 2019 09:15:10: 24000000 INFO @ Mon, 03 Jun 2019 09:15:13: 24000000 INFO @ Mon, 03 Jun 2019 09:15:18: 31000000 INFO @ Mon, 03 Jun 2019 09:15:23: 25000000 INFO @ Mon, 03 Jun 2019 09:15:25: 25000000 INFO @ Mon, 03 Jun 2019 09:15:28: 32000000 INFO @ Mon, 03 Jun 2019 09:15:36: 26000000 INFO @ Mon, 03 Jun 2019 09:15:38: 26000000 INFO @ Mon, 03 Jun 2019 09:15:38: 33000000 INFO @ Mon, 03 Jun 2019 09:15:48: 27000000 INFO @ Mon, 03 Jun 2019 09:15:49: 34000000 INFO @ Mon, 03 Jun 2019 09:15:51: 27000000 INFO @ Mon, 03 Jun 2019 09:15:59: 35000000 INFO @ Mon, 03 Jun 2019 09:16:01: 28000000 INFO @ Mon, 03 Jun 2019 09:16:03: 28000000 INFO @ Mon, 03 Jun 2019 09:16:09: 36000000 INFO @ Mon, 03 Jun 2019 09:16:14: 29000000 INFO @ Mon, 03 Jun 2019 09:16:16: 29000000 INFO @ Mon, 03 Jun 2019 09:16:19: 37000000 INFO @ Mon, 03 Jun 2019 09:16:26: 30000000 INFO @ Mon, 03 Jun 2019 09:16:29: 30000000 INFO @ Mon, 03 Jun 2019 09:16:30: 38000000 INFO @ Mon, 03 Jun 2019 09:16:39: 31000000 INFO @ Mon, 03 Jun 2019 09:16:40: 39000000 INFO @ Mon, 03 Jun 2019 09:16:43: 31000000 INFO @ Mon, 03 Jun 2019 09:16:50: 40000000 INFO @ Mon, 03 Jun 2019 09:16:51: 32000000 INFO @ Mon, 03 Jun 2019 09:16:56: 32000000 INFO @ Mon, 03 Jun 2019 09:17:00: 41000000 INFO @ Mon, 03 Jun 2019 09:17:04: 33000000 INFO @ Mon, 03 Jun 2019 09:17:09: 33000000 INFO @ Mon, 03 Jun 2019 09:17:10: 42000000 INFO @ Mon, 03 Jun 2019 09:17:16: 34000000 INFO @ Mon, 03 Jun 2019 09:17:20: 43000000 INFO @ Mon, 03 Jun 2019 09:17:22: 34000000 INFO @ Mon, 03 Jun 2019 09:17:28: 35000000 INFO @ Mon, 03 Jun 2019 09:17:31: 44000000 INFO @ Mon, 03 Jun 2019 09:17:34: 35000000 INFO @ Mon, 03 Jun 2019 09:17:41: 36000000 INFO @ Mon, 03 Jun 2019 09:17:41: 45000000 INFO @ Mon, 03 Jun 2019 09:17:47: 36000000 INFO @ Mon, 03 Jun 2019 09:17:52: 46000000 INFO @ Mon, 03 Jun 2019 09:17:54: 37000000 INFO @ Mon, 03 Jun 2019 09:18:00: 37000000 INFO @ Mon, 03 Jun 2019 09:18:02: 47000000 INFO @ Mon, 03 Jun 2019 09:18:06: 38000000 INFO @ Mon, 03 Jun 2019 09:18:12: 38000000 INFO @ Mon, 03 Jun 2019 09:18:13: 48000000 INFO @ Mon, 03 Jun 2019 09:18:18: 39000000 INFO @ Mon, 03 Jun 2019 09:18:24: 49000000 INFO @ Mon, 03 Jun 2019 09:18:25: 39000000 INFO @ Mon, 03 Jun 2019 09:18:31: 40000000 INFO @ Mon, 03 Jun 2019 09:18:34: 50000000 INFO @ Mon, 03 Jun 2019 09:18:37: 40000000 INFO @ Mon, 03 Jun 2019 09:18:43: 41000000 INFO @ Mon, 03 Jun 2019 09:18:44: 51000000 INFO @ Mon, 03 Jun 2019 09:18:50: 41000000 INFO @ Mon, 03 Jun 2019 09:18:54: 52000000 INFO @ Mon, 03 Jun 2019 09:18:55: 42000000 INFO @ Mon, 03 Jun 2019 09:19:02: 42000000 INFO @ Mon, 03 Jun 2019 09:19:04: 53000000 INFO @ Mon, 03 Jun 2019 09:19:07: 43000000 INFO @ Mon, 03 Jun 2019 09:19:14: 54000000 INFO @ Mon, 03 Jun 2019 09:19:14: 43000000 INFO @ Mon, 03 Jun 2019 09:19:19: 44000000 INFO @ Mon, 03 Jun 2019 09:19:24: 55000000 INFO @ Mon, 03 Jun 2019 09:19:27: 44000000 INFO @ Mon, 03 Jun 2019 09:19:31: 45000000 INFO @ Mon, 03 Jun 2019 09:19:34: 56000000 INFO @ Mon, 03 Jun 2019 09:19:39: 45000000 INFO @ Mon, 03 Jun 2019 09:19:44: 46000000 INFO @ Mon, 03 Jun 2019 09:19:44: 57000000 INFO @ Mon, 03 Jun 2019 09:19:51: 46000000 INFO @ Mon, 03 Jun 2019 09:19:55: 58000000 INFO @ Mon, 03 Jun 2019 09:19:56: 47000000 INFO @ Mon, 03 Jun 2019 09:20:03: 47000000 INFO @ Mon, 03 Jun 2019 09:20:05: 59000000 INFO @ Mon, 03 Jun 2019 09:20:08: 48000000 INFO @ Mon, 03 Jun 2019 09:20:15: 60000000 INFO @ Mon, 03 Jun 2019 09:20:16: 48000000 INFO @ Mon, 03 Jun 2019 09:20:20: 49000000 INFO @ Mon, 03 Jun 2019 09:20:25: 61000000 INFO @ Mon, 03 Jun 2019 09:20:28: 49000000 INFO @ Mon, 03 Jun 2019 09:20:33: 50000000 INFO @ Mon, 03 Jun 2019 09:20:35: 62000000 INFO @ Mon, 03 Jun 2019 09:20:40: 50000000 INFO @ Mon, 03 Jun 2019 09:20:45: 51000000 INFO @ Mon, 03 Jun 2019 09:20:45: 63000000 INFO @ Mon, 03 Jun 2019 09:20:53: 51000000 INFO @ Mon, 03 Jun 2019 09:20:56: 64000000 INFO @ Mon, 03 Jun 2019 09:20:57: 52000000 INFO @ Mon, 03 Jun 2019 09:21:06: 52000000 INFO @ Mon, 03 Jun 2019 09:21:06: 65000000 INFO @ Mon, 03 Jun 2019 09:21:09: 53000000 INFO @ Mon, 03 Jun 2019 09:21:16: 66000000 INFO @ Mon, 03 Jun 2019 09:21:18: 53000000 INFO @ Mon, 03 Jun 2019 09:21:21: 54000000 INFO @ Mon, 03 Jun 2019 09:21:27: 67000000 INFO @ Mon, 03 Jun 2019 09:21:31: 54000000 INFO @ Mon, 03 Jun 2019 09:21:34: 55000000 INFO @ Mon, 03 Jun 2019 09:21:37: 68000000 INFO @ Mon, 03 Jun 2019 09:21:44: 55000000 INFO @ Mon, 03 Jun 2019 09:21:45: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 09:21:45: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 09:21:45: #1 total tags in treatment: 32904990 INFO @ Mon, 03 Jun 2019 09:21:45: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 09:21:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 09:21:45: #1 tags after filtering in treatment: 29732003 INFO @ Mon, 03 Jun 2019 09:21:45: #1 Redundant rate of treatment: 0.10 INFO @ Mon, 03 Jun 2019 09:21:45: #1 finished! INFO @ Mon, 03 Jun 2019 09:21:45: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 09:21:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 09:21:46: 56000000 INFO @ Mon, 03 Jun 2019 09:21:48: #2 number of paired peaks: 133 WARNING @ Mon, 03 Jun 2019 09:21:48: Fewer paired peaks (133) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 133 pairs to build model! INFO @ Mon, 03 Jun 2019 09:21:48: start model_add_line... INFO @ Mon, 03 Jun 2019 09:21:48: start X-correlation... INFO @ Mon, 03 Jun 2019 09:21:48: end of X-cor INFO @ Mon, 03 Jun 2019 09:21:48: #2 finished! INFO @ Mon, 03 Jun 2019 09:21:48: #2 predicted fragment length is 163 bps INFO @ Mon, 03 Jun 2019 09:21:48: #2 alternative fragment length(s) may be 163 bps INFO @ Mon, 03 Jun 2019 09:21:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.20_model.r INFO @ Mon, 03 Jun 2019 09:21:48: #3 Call peaks... INFO @ Mon, 03 Jun 2019 09:21:48: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 09:21:57: 56000000 INFO @ Mon, 03 Jun 2019 09:21:59: 57000000 INFO @ Mon, 03 Jun 2019 09:22:10: 57000000 INFO @ Mon, 03 Jun 2019 09:22:11: 58000000 INFO @ Mon, 03 Jun 2019 09:22:23: 58000000 INFO @ Mon, 03 Jun 2019 09:22:24: 59000000 INFO @ Mon, 03 Jun 2019 09:22:36: 59000000 INFO @ Mon, 03 Jun 2019 09:22:36: 60000000 INFO @ Mon, 03 Jun 2019 09:22:48: 61000000 INFO @ Mon, 03 Jun 2019 09:22:48: 60000000 INFO @ Mon, 03 Jun 2019 09:23:00: 62000000 INFO @ Mon, 03 Jun 2019 09:23:01: 61000000 INFO @ Mon, 03 Jun 2019 09:23:01: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 09:23:12: 63000000 INFO @ Mon, 03 Jun 2019 09:23:14: 62000000 INFO @ Mon, 03 Jun 2019 09:23:24: 64000000 INFO @ Mon, 03 Jun 2019 09:23:26: 63000000 INFO @ Mon, 03 Jun 2019 09:23:35: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.20_peaks.xls INFO @ Mon, 03 Jun 2019 09:23:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 09:23:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.20_summits.bed INFO @ Mon, 03 Jun 2019 09:23:35: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (913 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 09:23:36: 65000000 INFO @ Mon, 03 Jun 2019 09:23:39: 64000000 INFO @ Mon, 03 Jun 2019 09:23:48: 66000000 INFO @ Mon, 03 Jun 2019 09:23:51: 65000000 INFO @ Mon, 03 Jun 2019 09:24:00: 67000000 INFO @ Mon, 03 Jun 2019 09:24:03: 66000000 INFO @ Mon, 03 Jun 2019 09:24:13: 68000000 INFO @ Mon, 03 Jun 2019 09:24:16: 67000000 INFO @ Mon, 03 Jun 2019 09:24:24: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 09:24:24: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 09:24:24: #1 total tags in treatment: 32904990 INFO @ Mon, 03 Jun 2019 09:24:24: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 09:24:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 09:24:24: #1 tags after filtering in treatment: 29732003 INFO @ Mon, 03 Jun 2019 09:24:24: #1 Redundant rate of treatment: 0.10 INFO @ Mon, 03 Jun 2019 09:24:24: #1 finished! INFO @ Mon, 03 Jun 2019 09:24:24: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 09:24:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 09:24:27: #2 number of paired peaks: 133 WARNING @ Mon, 03 Jun 2019 09:24:27: Fewer paired peaks (133) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 133 pairs to build model! INFO @ Mon, 03 Jun 2019 09:24:27: start model_add_line... INFO @ Mon, 03 Jun 2019 09:24:27: start X-correlation... INFO @ Mon, 03 Jun 2019 09:24:27: end of X-cor INFO @ Mon, 03 Jun 2019 09:24:27: #2 finished! INFO @ Mon, 03 Jun 2019 09:24:27: #2 predicted fragment length is 163 bps INFO @ Mon, 03 Jun 2019 09:24:27: #2 alternative fragment length(s) may be 163 bps INFO @ Mon, 03 Jun 2019 09:24:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.05_model.r INFO @ Mon, 03 Jun 2019 09:24:27: #3 Call peaks... INFO @ Mon, 03 Jun 2019 09:24:27: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 09:24:28: 68000000 INFO @ Mon, 03 Jun 2019 09:24:37: #1 tag size is determined as 51 bps INFO @ Mon, 03 Jun 2019 09:24:37: #1 tag size = 51 INFO @ Mon, 03 Jun 2019 09:24:37: #1 total tags in treatment: 32904990 INFO @ Mon, 03 Jun 2019 09:24:37: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 09:24:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 09:24:37: #1 tags after filtering in treatment: 29732003 INFO @ Mon, 03 Jun 2019 09:24:37: #1 Redundant rate of treatment: 0.10 INFO @ Mon, 03 Jun 2019 09:24:37: #1 finished! INFO @ Mon, 03 Jun 2019 09:24:37: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 09:24:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 09:24:40: #2 number of paired peaks: 133 WARNING @ Mon, 03 Jun 2019 09:24:40: Fewer paired peaks (133) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 133 pairs to build model! INFO @ Mon, 03 Jun 2019 09:24:40: start model_add_line... INFO @ Mon, 03 Jun 2019 09:24:40: start X-correlation... INFO @ Mon, 03 Jun 2019 09:24:40: end of X-cor INFO @ Mon, 03 Jun 2019 09:24:40: #2 finished! INFO @ Mon, 03 Jun 2019 09:24:40: #2 predicted fragment length is 163 bps INFO @ Mon, 03 Jun 2019 09:24:40: #2 alternative fragment length(s) may be 163 bps INFO @ Mon, 03 Jun 2019 09:24:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.10_model.r INFO @ Mon, 03 Jun 2019 09:24:40: #3 Call peaks... INFO @ Mon, 03 Jun 2019 09:24:40: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 09:25:40: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 09:25:54: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 09:26:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.05_peaks.xls INFO @ Mon, 03 Jun 2019 09:26:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 09:26:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.05_summits.bed INFO @ Mon, 03 Jun 2019 09:26:14: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2789 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 09:26:27: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.10_peaks.xls INFO @ Mon, 03 Jun 2019 09:26:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 09:26:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX2612512/SRX2612512.10_summits.bed INFO @ Mon, 03 Jun 2019 09:26:27: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1723 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。