Job ID = 6527736 SRX = SRX2592424 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:41:29 prefetch.2.10.7: 1) Downloading 'SRR5289701'... 2020-06-29T13:41:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:45:58 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:45:58 prefetch.2.10.7: 1) 'SRR5289701' was downloaded successfully Read 24424359 spots for SRR5289701/SRR5289701.sra Written 24424359 spots for SRR5289701/SRR5289701.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:16 24424359 reads; of these: 24424359 (100.00%) were unpaired; of these: 1658572 (6.79%) aligned 0 times 16759032 (68.62%) aligned exactly 1 time 6006755 (24.59%) aligned >1 times 93.21% overall alignment rate Time searching: 00:10:16 Overall time: 00:10:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5411890 / 22765787 = 0.2377 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:10:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:10:27: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:10:27: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:10:34: 1000000 INFO @ Mon, 29 Jun 2020 23:10:41: 2000000 INFO @ Mon, 29 Jun 2020 23:10:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:10:55: 4000000 INFO @ Mon, 29 Jun 2020 23:10:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:10:57: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:10:57: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:11:02: 5000000 INFO @ Mon, 29 Jun 2020 23:11:05: 1000000 INFO @ Mon, 29 Jun 2020 23:11:10: 6000000 INFO @ Mon, 29 Jun 2020 23:11:13: 2000000 INFO @ Mon, 29 Jun 2020 23:11:17: 7000000 INFO @ Mon, 29 Jun 2020 23:11:20: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:11:25: 8000000 INFO @ Mon, 29 Jun 2020 23:11:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:11:27: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:11:27: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:11:28: 4000000 INFO @ Mon, 29 Jun 2020 23:11:33: 9000000 INFO @ Mon, 29 Jun 2020 23:11:35: 1000000 INFO @ Mon, 29 Jun 2020 23:11:36: 5000000 INFO @ Mon, 29 Jun 2020 23:11:41: 10000000 INFO @ Mon, 29 Jun 2020 23:11:42: 2000000 INFO @ Mon, 29 Jun 2020 23:11:44: 6000000 INFO @ Mon, 29 Jun 2020 23:11:49: 11000000 INFO @ Mon, 29 Jun 2020 23:11:50: 3000000 INFO @ Mon, 29 Jun 2020 23:11:51: 7000000 INFO @ Mon, 29 Jun 2020 23:11:57: 12000000 INFO @ Mon, 29 Jun 2020 23:11:57: 4000000 INFO @ Mon, 29 Jun 2020 23:11:59: 8000000 INFO @ Mon, 29 Jun 2020 23:12:05: 5000000 INFO @ Mon, 29 Jun 2020 23:12:05: 13000000 INFO @ Mon, 29 Jun 2020 23:12:07: 9000000 INFO @ Mon, 29 Jun 2020 23:12:12: 6000000 INFO @ Mon, 29 Jun 2020 23:12:13: 14000000 INFO @ Mon, 29 Jun 2020 23:12:14: 10000000 INFO @ Mon, 29 Jun 2020 23:12:20: 7000000 INFO @ Mon, 29 Jun 2020 23:12:21: 15000000 INFO @ Mon, 29 Jun 2020 23:12:22: 11000000 INFO @ Mon, 29 Jun 2020 23:12:27: 8000000 INFO @ Mon, 29 Jun 2020 23:12:28: 16000000 INFO @ Mon, 29 Jun 2020 23:12:29: 12000000 INFO @ Mon, 29 Jun 2020 23:12:35: 9000000 INFO @ Mon, 29 Jun 2020 23:12:36: 17000000 INFO @ Mon, 29 Jun 2020 23:12:37: 13000000 INFO @ Mon, 29 Jun 2020 23:12:39: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:12:39: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:12:39: #1 total tags in treatment: 17353897 INFO @ Mon, 29 Jun 2020 23:12:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:12:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:12:39: #1 tags after filtering in treatment: 17353897 INFO @ Mon, 29 Jun 2020 23:12:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:12:39: #1 finished! INFO @ Mon, 29 Jun 2020 23:12:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:12:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:12:40: #2 number of paired peaks: 88 WARNING @ Mon, 29 Jun 2020 23:12:40: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:12:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:12:42: 10000000 INFO @ Mon, 29 Jun 2020 23:12:44: 14000000 INFO @ Mon, 29 Jun 2020 23:12:49: 11000000 INFO @ Mon, 29 Jun 2020 23:12:51: 15000000 INFO @ Mon, 29 Jun 2020 23:12:56: 12000000 INFO @ Mon, 29 Jun 2020 23:12:58: 16000000 INFO @ Mon, 29 Jun 2020 23:13:04: 13000000 INFO @ Mon, 29 Jun 2020 23:13:05: 17000000 INFO @ Mon, 29 Jun 2020 23:13:07: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:13:07: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:13:07: #1 total tags in treatment: 17353897 INFO @ Mon, 29 Jun 2020 23:13:07: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:13:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:13:08: #1 tags after filtering in treatment: 17353897 INFO @ Mon, 29 Jun 2020 23:13:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:13:08: #1 finished! INFO @ Mon, 29 Jun 2020 23:13:08: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:13:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:13:09: #2 number of paired peaks: 88 WARNING @ Mon, 29 Jun 2020 23:13:09: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:13:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:13:11: 14000000 INFO @ Mon, 29 Jun 2020 23:13:17: 15000000 INFO @ Mon, 29 Jun 2020 23:13:24: 16000000 INFO @ Mon, 29 Jun 2020 23:13:30: 17000000 INFO @ Mon, 29 Jun 2020 23:13:33: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:13:33: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:13:33: #1 total tags in treatment: 17353897 INFO @ Mon, 29 Jun 2020 23:13:33: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:13:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:13:33: #1 tags after filtering in treatment: 17353897 INFO @ Mon, 29 Jun 2020 23:13:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:13:33: #1 finished! INFO @ Mon, 29 Jun 2020 23:13:33: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:13:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:13:34: #2 number of paired peaks: 88 WARNING @ Mon, 29 Jun 2020 23:13:34: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:13:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592424/SRX2592424.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。