Job ID = 6527734 SRX = SRX2592421 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:17:49 prefetch.2.10.7: 1) Downloading 'SRR5289698'... 2020-06-29T13:17:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:21:03 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:21:03 prefetch.2.10.7: 1) 'SRR5289698' was downloaded successfully Read 26242199 spots for SRR5289698/SRR5289698.sra Written 26242199 spots for SRR5289698/SRR5289698.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:32 26242199 reads; of these: 26242199 (100.00%) were unpaired; of these: 1780740 (6.79%) aligned 0 times 17898754 (68.21%) aligned exactly 1 time 6562705 (25.01%) aligned >1 times 93.21% overall alignment rate Time searching: 00:10:32 Overall time: 00:10:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4477465 / 24461459 = 0.1830 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:46:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:46:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:46:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:46:08: 1000000 INFO @ Mon, 29 Jun 2020 22:46:14: 2000000 INFO @ Mon, 29 Jun 2020 22:46:21: 3000000 INFO @ Mon, 29 Jun 2020 22:46:27: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:46:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:46:31: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:46:31: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:46:33: 5000000 INFO @ Mon, 29 Jun 2020 22:46:38: 1000000 INFO @ Mon, 29 Jun 2020 22:46:40: 6000000 INFO @ Mon, 29 Jun 2020 22:46:45: 2000000 INFO @ Mon, 29 Jun 2020 22:46:47: 7000000 INFO @ Mon, 29 Jun 2020 22:46:52: 3000000 INFO @ Mon, 29 Jun 2020 22:46:54: 8000000 INFO @ Mon, 29 Jun 2020 22:46:58: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:47:01: 9000000 INFO @ Mon, 29 Jun 2020 22:47:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:47:01: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:47:01: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:47:05: 5000000 INFO @ Mon, 29 Jun 2020 22:47:08: 10000000 INFO @ Mon, 29 Jun 2020 22:47:08: 1000000 INFO @ Mon, 29 Jun 2020 22:47:12: 6000000 INFO @ Mon, 29 Jun 2020 22:47:15: 11000000 INFO @ Mon, 29 Jun 2020 22:47:15: 2000000 INFO @ Mon, 29 Jun 2020 22:47:19: 7000000 INFO @ Mon, 29 Jun 2020 22:47:22: 12000000 INFO @ Mon, 29 Jun 2020 22:47:22: 3000000 INFO @ Mon, 29 Jun 2020 22:47:26: 8000000 INFO @ Mon, 29 Jun 2020 22:47:28: 13000000 INFO @ Mon, 29 Jun 2020 22:47:29: 4000000 INFO @ Mon, 29 Jun 2020 22:47:33: 9000000 INFO @ Mon, 29 Jun 2020 22:47:35: 14000000 INFO @ Mon, 29 Jun 2020 22:47:35: 5000000 INFO @ Mon, 29 Jun 2020 22:47:39: 10000000 INFO @ Mon, 29 Jun 2020 22:47:42: 15000000 INFO @ Mon, 29 Jun 2020 22:47:42: 6000000 INFO @ Mon, 29 Jun 2020 22:47:46: 11000000 INFO @ Mon, 29 Jun 2020 22:47:49: 16000000 INFO @ Mon, 29 Jun 2020 22:47:49: 7000000 INFO @ Mon, 29 Jun 2020 22:47:53: 12000000 INFO @ Mon, 29 Jun 2020 22:47:56: 17000000 INFO @ Mon, 29 Jun 2020 22:47:56: 8000000 INFO @ Mon, 29 Jun 2020 22:48:00: 13000000 INFO @ Mon, 29 Jun 2020 22:48:02: 18000000 INFO @ Mon, 29 Jun 2020 22:48:03: 9000000 INFO @ Mon, 29 Jun 2020 22:48:06: 14000000 INFO @ Mon, 29 Jun 2020 22:48:09: 19000000 INFO @ Mon, 29 Jun 2020 22:48:09: 10000000 INFO @ Mon, 29 Jun 2020 22:48:13: 15000000 INFO @ Mon, 29 Jun 2020 22:48:16: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:48:16: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:48:16: #1 total tags in treatment: 19983994 INFO @ Mon, 29 Jun 2020 22:48:16: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:48:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:48:16: #1 tags after filtering in treatment: 19983994 INFO @ Mon, 29 Jun 2020 22:48:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:48:16: #1 finished! INFO @ Mon, 29 Jun 2020 22:48:16: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:48:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:48:16: 11000000 INFO @ Mon, 29 Jun 2020 22:48:17: #2 number of paired peaks: 80 WARNING @ Mon, 29 Jun 2020 22:48:17: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:48:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:48:20: 16000000 INFO @ Mon, 29 Jun 2020 22:48:23: 12000000 INFO @ Mon, 29 Jun 2020 22:48:27: 17000000 INFO @ Mon, 29 Jun 2020 22:48:29: 13000000 INFO @ Mon, 29 Jun 2020 22:48:33: 18000000 INFO @ Mon, 29 Jun 2020 22:48:36: 14000000 INFO @ Mon, 29 Jun 2020 22:48:40: 19000000 INFO @ Mon, 29 Jun 2020 22:48:43: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:48:47: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:48:47: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:48:47: #1 total tags in treatment: 19983994 INFO @ Mon, 29 Jun 2020 22:48:47: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:48:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:48:47: #1 tags after filtering in treatment: 19983994 INFO @ Mon, 29 Jun 2020 22:48:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:48:47: #1 finished! INFO @ Mon, 29 Jun 2020 22:48:47: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:48:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:48:48: #2 number of paired peaks: 80 WARNING @ Mon, 29 Jun 2020 22:48:48: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:48:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:48:49: 16000000 INFO @ Mon, 29 Jun 2020 22:48:56: 17000000 INFO @ Mon, 29 Jun 2020 22:49:02: 18000000 INFO @ Mon, 29 Jun 2020 22:49:09: 19000000 INFO @ Mon, 29 Jun 2020 22:49:15: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:49:15: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:49:15: #1 total tags in treatment: 19983994 INFO @ Mon, 29 Jun 2020 22:49:15: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:49:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:49:15: #1 tags after filtering in treatment: 19983994 INFO @ Mon, 29 Jun 2020 22:49:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:49:15: #1 finished! INFO @ Mon, 29 Jun 2020 22:49:15: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:49:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:49:16: #2 number of paired peaks: 80 WARNING @ Mon, 29 Jun 2020 22:49:16: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:49:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2592421/SRX2592421.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。