Job ID = 11171190 sra ファイルのダウンロード中... Completed: 1286883K bytes transferred in 64 seconds (163675K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 43923803 spots for /home/okishinya/chipatlas/results/dm3/SRX2564145/SRR5259264.sra Written 43923803 spots for /home/okishinya/chipatlas/results/dm3/SRX2564145/SRR5259264.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:39 43923803 reads; of these: 43923803 (100.00%) were unpaired; of these: 7333010 (16.69%) aligned 0 times 26467324 (60.26%) aligned exactly 1 time 10123469 (23.05%) aligned >1 times 83.31% overall alignment rate Time searching: 00:20:39 Overall time: 00:20:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7957605 / 36590793 = 0.2175 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:15:59: # Command line: callpeak -t SRX2564145.bam -f BAM -g dm -n SRX2564145.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2564145.10 # format = BAM # ChIP-seq file = ['SRX2564145.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:15:59: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:15:59: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:15:59: # Command line: callpeak -t SRX2564145.bam -f BAM -g dm -n SRX2564145.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2564145.20 # format = BAM # ChIP-seq file = ['SRX2564145.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:15:59: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:15:59: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:15:59: # Command line: callpeak -t SRX2564145.bam -f BAM -g dm -n SRX2564145.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2564145.05 # format = BAM # ChIP-seq file = ['SRX2564145.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:15:59: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:15:59: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:16:05: 1000000 INFO @ Sat, 08 Sep 2018 13:16:06: 1000000 INFO @ Sat, 08 Sep 2018 13:16:06: 1000000 INFO @ Sat, 08 Sep 2018 13:16:12: 2000000 INFO @ Sat, 08 Sep 2018 13:16:12: 2000000 INFO @ Sat, 08 Sep 2018 13:16:13: 2000000 INFO @ Sat, 08 Sep 2018 13:16:19: 3000000 INFO @ Sat, 08 Sep 2018 13:16:19: 3000000 INFO @ Sat, 08 Sep 2018 13:16:19: 3000000 INFO @ Sat, 08 Sep 2018 13:16:25: 4000000 INFO @ Sat, 08 Sep 2018 13:16:26: 4000000 INFO @ Sat, 08 Sep 2018 13:16:26: 4000000 INFO @ Sat, 08 Sep 2018 13:16:32: 5000000 INFO @ Sat, 08 Sep 2018 13:16:32: 5000000 INFO @ Sat, 08 Sep 2018 13:16:33: 5000000 INFO @ Sat, 08 Sep 2018 13:16:39: 6000000 INFO @ Sat, 08 Sep 2018 13:16:39: 6000000 INFO @ Sat, 08 Sep 2018 13:16:40: 6000000 INFO @ Sat, 08 Sep 2018 13:16:45: 7000000 INFO @ Sat, 08 Sep 2018 13:16:46: 7000000 INFO @ Sat, 08 Sep 2018 13:16:47: 7000000 INFO @ Sat, 08 Sep 2018 13:16:52: 8000000 INFO @ Sat, 08 Sep 2018 13:16:52: 8000000 INFO @ Sat, 08 Sep 2018 13:16:54: 8000000 INFO @ Sat, 08 Sep 2018 13:16:58: 9000000 INFO @ Sat, 08 Sep 2018 13:16:59: 9000000 INFO @ Sat, 08 Sep 2018 13:17:01: 9000000 INFO @ Sat, 08 Sep 2018 13:17:05: 10000000 INFO @ Sat, 08 Sep 2018 13:17:06: 10000000 INFO @ Sat, 08 Sep 2018 13:17:08: 10000000 INFO @ Sat, 08 Sep 2018 13:17:11: 11000000 INFO @ Sat, 08 Sep 2018 13:17:13: 11000000 INFO @ Sat, 08 Sep 2018 13:17:15: 11000000 INFO @ Sat, 08 Sep 2018 13:17:18: 12000000 INFO @ Sat, 08 Sep 2018 13:17:19: 12000000 INFO @ Sat, 08 Sep 2018 13:17:22: 12000000 INFO @ Sat, 08 Sep 2018 13:17:24: 13000000 INFO @ Sat, 08 Sep 2018 13:17:26: 13000000 INFO @ Sat, 08 Sep 2018 13:17:29: 13000000 INFO @ Sat, 08 Sep 2018 13:17:31: 14000000 INFO @ Sat, 08 Sep 2018 13:17:33: 14000000 INFO @ Sat, 08 Sep 2018 13:17:36: 14000000 INFO @ Sat, 08 Sep 2018 13:17:37: 15000000 INFO @ Sat, 08 Sep 2018 13:17:39: 15000000 INFO @ Sat, 08 Sep 2018 13:17:43: 15000000 INFO @ Sat, 08 Sep 2018 13:17:44: 16000000 INFO @ Sat, 08 Sep 2018 13:17:46: 16000000 INFO @ Sat, 08 Sep 2018 13:17:50: 17000000 INFO @ Sat, 08 Sep 2018 13:17:50: 16000000 INFO @ Sat, 08 Sep 2018 13:17:53: 17000000 INFO @ Sat, 08 Sep 2018 13:17:56: 18000000 INFO @ Sat, 08 Sep 2018 13:17:57: 17000000 INFO @ Sat, 08 Sep 2018 13:17:59: 18000000 INFO @ Sat, 08 Sep 2018 13:18:03: 19000000 INFO @ Sat, 08 Sep 2018 13:18:04: 18000000 INFO @ Sat, 08 Sep 2018 13:18:06: 19000000 INFO @ Sat, 08 Sep 2018 13:18:09: 20000000 INFO @ Sat, 08 Sep 2018 13:18:11: 19000000 INFO @ Sat, 08 Sep 2018 13:18:13: 20000000 INFO @ Sat, 08 Sep 2018 13:18:16: 21000000 INFO @ Sat, 08 Sep 2018 13:18:18: 20000000 INFO @ Sat, 08 Sep 2018 13:18:19: 21000000 INFO @ Sat, 08 Sep 2018 13:18:22: 22000000 INFO @ Sat, 08 Sep 2018 13:18:24: 21000000 INFO @ Sat, 08 Sep 2018 13:18:26: 22000000 INFO @ Sat, 08 Sep 2018 13:18:29: 23000000 INFO @ Sat, 08 Sep 2018 13:18:31: 22000000 INFO @ Sat, 08 Sep 2018 13:18:33: 23000000 INFO @ Sat, 08 Sep 2018 13:18:35: 24000000 INFO @ Sat, 08 Sep 2018 13:18:38: 23000000 INFO @ Sat, 08 Sep 2018 13:18:39: 24000000 INFO @ Sat, 08 Sep 2018 13:18:42: 25000000 INFO @ Sat, 08 Sep 2018 13:18:45: 24000000 INFO @ Sat, 08 Sep 2018 13:18:46: 25000000 INFO @ Sat, 08 Sep 2018 13:18:48: 26000000 INFO @ Sat, 08 Sep 2018 13:18:52: 25000000 INFO @ Sat, 08 Sep 2018 13:18:53: 26000000 INFO @ Sat, 08 Sep 2018 13:18:55: 27000000 INFO @ Sat, 08 Sep 2018 13:18:59: 26000000 INFO @ Sat, 08 Sep 2018 13:19:00: 27000000 INFO @ Sat, 08 Sep 2018 13:19:02: 28000000 INFO @ Sat, 08 Sep 2018 13:19:06: 27000000 INFO @ Sat, 08 Sep 2018 13:19:06: #1 tag size is determined as 68 bps INFO @ Sat, 08 Sep 2018 13:19:06: #1 tag size = 68 INFO @ Sat, 08 Sep 2018 13:19:06: #1 total tags in treatment: 28633188 INFO @ Sat, 08 Sep 2018 13:19:06: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:19:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:19:06: 28000000 INFO @ Sat, 08 Sep 2018 13:19:07: #1 tags after filtering in treatment: 28633188 INFO @ Sat, 08 Sep 2018 13:19:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:19:07: #1 finished! INFO @ Sat, 08 Sep 2018 13:19:07: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:19:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:19:08: #2 number of paired peaks: 50 WARNING @ Sat, 08 Sep 2018 13:19:08: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:19:08: Process for pairing-model is terminated! cat: SRX2564145.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2564145.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2564145.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2564145.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:19:11: #1 tag size is determined as 68 bps INFO @ Sat, 08 Sep 2018 13:19:11: #1 tag size = 68 INFO @ Sat, 08 Sep 2018 13:19:11: #1 total tags in treatment: 28633188 INFO @ Sat, 08 Sep 2018 13:19:11: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:19:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:19:11: #1 tags after filtering in treatment: 28633188 INFO @ Sat, 08 Sep 2018 13:19:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:19:11: #1 finished! INFO @ Sat, 08 Sep 2018 13:19:11: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:19:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:19:13: 28000000 INFO @ Sat, 08 Sep 2018 13:19:13: #2 number of paired peaks: 50 WARNING @ Sat, 08 Sep 2018 13:19:13: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:19:13: Process for pairing-model is terminated! cat: SRX2564145.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2564145.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2564145.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2564145.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:19:17: #1 tag size is determined as 68 bps INFO @ Sat, 08 Sep 2018 13:19:17: #1 tag size = 68 INFO @ Sat, 08 Sep 2018 13:19:17: #1 total tags in treatment: 28633188 INFO @ Sat, 08 Sep 2018 13:19:17: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:19:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:19:18: #1 tags after filtering in treatment: 28633188 INFO @ Sat, 08 Sep 2018 13:19:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:19:18: #1 finished! INFO @ Sat, 08 Sep 2018 13:19:18: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:19:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:19:20: #2 number of paired peaks: 50 WARNING @ Sat, 08 Sep 2018 13:19:20: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:19:20: Process for pairing-model is terminated! cat: SRX2564145.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2564145.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2564145.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2564145.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。