Job ID = 9371927 sra ファイルのダウンロード中... Completed: 1126204K bytes transferred in 35 seconds (258754K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 23897334 spots for /home/okishinya/chipatlas/results/dm3/SRX2520650/SRR5206769.sra Written 23897334 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:41 23897334 reads; of these: 23897334 (100.00%) were unpaired; of these: 476271 (1.99%) aligned 0 times 16725529 (69.99%) aligned exactly 1 time 6695534 (28.02%) aligned >1 times 98.01% overall alignment rate Time searching: 00:10:41 Overall time: 00:10:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5407618 / 23421063 = 0.2309 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 04 Aug 2017 15:06:42: # Command line: callpeak -t SRX2520650.bam -f BAM -g dm -n SRX2520650.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2520650.20 # format = BAM # ChIP-seq file = ['SRX2520650.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 04 Aug 2017 15:06:42: #1 read tag files... INFO @ Fri, 04 Aug 2017 15:06:42: #1 read treatment tags... INFO @ Fri, 04 Aug 2017 15:06:42: # Command line: callpeak -t SRX2520650.bam -f BAM -g dm -n SRX2520650.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2520650.05 # format = BAM # ChIP-seq file = ['SRX2520650.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 04 Aug 2017 15:06:42: #1 read tag files... INFO @ Fri, 04 Aug 2017 15:06:42: #1 read treatment tags... INFO @ Fri, 04 Aug 2017 15:06:42: # Command line: callpeak -t SRX2520650.bam -f BAM -g dm -n SRX2520650.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2520650.10 # format = BAM # ChIP-seq file = ['SRX2520650.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 04 Aug 2017 15:06:42: #1 read tag files... INFO @ Fri, 04 Aug 2017 15:06:42: #1 read treatment tags... INFO @ Fri, 04 Aug 2017 15:06:49: 1000000 INFO @ Fri, 04 Aug 2017 15:06:49: 1000000 INFO @ Fri, 04 Aug 2017 15:06:49: 1000000 INFO @ Fri, 04 Aug 2017 15:06:55: 2000000 INFO @ Fri, 04 Aug 2017 15:06:55: 2000000 INFO @ Fri, 04 Aug 2017 15:06:56: 2000000 INFO @ Fri, 04 Aug 2017 15:07:02: 3000000 INFO @ Fri, 04 Aug 2017 15:07:02: 3000000 INFO @ Fri, 04 Aug 2017 15:07:04: 3000000 INFO @ Fri, 04 Aug 2017 15:07:10: 4000000 INFO @ Fri, 04 Aug 2017 15:07:10: 4000000 INFO @ Fri, 04 Aug 2017 15:07:12: 4000000 INFO @ Fri, 04 Aug 2017 15:07:17: 5000000 INFO @ Fri, 04 Aug 2017 15:07:17: 5000000 INFO @ Fri, 04 Aug 2017 15:07:20: 5000000 INFO @ Fri, 04 Aug 2017 15:07:24: 6000000 INFO @ Fri, 04 Aug 2017 15:07:24: 6000000 INFO @ Fri, 04 Aug 2017 15:07:27: 6000000 INFO @ Fri, 04 Aug 2017 15:07:32: 7000000 INFO @ Fri, 04 Aug 2017 15:07:32: 7000000 INFO @ Fri, 04 Aug 2017 15:07:35: 7000000 INFO @ Fri, 04 Aug 2017 15:07:39: 8000000 INFO @ Fri, 04 Aug 2017 15:07:39: 8000000 INFO @ Fri, 04 Aug 2017 15:07:43: 8000000 INFO @ Fri, 04 Aug 2017 15:07:47: 9000000 INFO @ Fri, 04 Aug 2017 15:07:47: 9000000 INFO @ Fri, 04 Aug 2017 15:07:51: 9000000 INFO @ Fri, 04 Aug 2017 15:07:54: 10000000 INFO @ Fri, 04 Aug 2017 15:07:54: 10000000 INFO @ Fri, 04 Aug 2017 15:07:59: 10000000 INFO @ Fri, 04 Aug 2017 15:08:01: 11000000 INFO @ Fri, 04 Aug 2017 15:08:01: 11000000 INFO @ Fri, 04 Aug 2017 15:08:05: 11000000 INFO @ Fri, 04 Aug 2017 15:08:08: 12000000 INFO @ Fri, 04 Aug 2017 15:08:08: 12000000 INFO @ Fri, 04 Aug 2017 15:08:11: 12000000 INFO @ Fri, 04 Aug 2017 15:08:15: 13000000 INFO @ Fri, 04 Aug 2017 15:08:15: 13000000 INFO @ Fri, 04 Aug 2017 15:08:18: 13000000 INFO @ Fri, 04 Aug 2017 15:08:23: 14000000 INFO @ Fri, 04 Aug 2017 15:08:23: 14000000 INFO @ Fri, 04 Aug 2017 15:08:24: 14000000 INFO @ Fri, 04 Aug 2017 15:08:30: 15000000 INFO @ Fri, 04 Aug 2017 15:08:30: 15000000 INFO @ Fri, 04 Aug 2017 15:08:30: 15000000 INFO @ Fri, 04 Aug 2017 15:08:36: 16000000 INFO @ Fri, 04 Aug 2017 15:08:37: 16000000 INFO @ Fri, 04 Aug 2017 15:08:37: 16000000 INFO @ Fri, 04 Aug 2017 15:08:43: 17000000 INFO @ Fri, 04 Aug 2017 15:08:44: 17000000 INFO @ Fri, 04 Aug 2017 15:08:44: 17000000 INFO @ Fri, 04 Aug 2017 15:08:48: 18000000 INFO @ Fri, 04 Aug 2017 15:08:49: #1 tag size is determined as 50 bps INFO @ Fri, 04 Aug 2017 15:08:49: #1 tag size = 50 INFO @ Fri, 04 Aug 2017 15:08:49: #1 total tags in treatment: 18013445 INFO @ Fri, 04 Aug 2017 15:08:49: #1 user defined the maximum tags... INFO @ Fri, 04 Aug 2017 15:08:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 04 Aug 2017 15:08:49: #1 tags after filtering in treatment: 18013445 INFO @ Fri, 04 Aug 2017 15:08:49: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 04 Aug 2017 15:08:49: #1 finished! INFO @ Fri, 04 Aug 2017 15:08:49: #2 Build Peak Model... INFO @ Fri, 04 Aug 2017 15:08:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 04 Aug 2017 15:08:50: #2 number of paired peaks: 13 WARNING @ Fri, 04 Aug 2017 15:08:50: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 04 Aug 2017 15:08:50: Process for pairing-model is terminated! cat: SRX2520650.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2520650.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2520650.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2520650.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 04 Aug 2017 15:08:51: 18000000 INFO @ Fri, 04 Aug 2017 15:08:51: 18000000 INFO @ Fri, 04 Aug 2017 15:08:52: #1 tag size is determined as 50 bps INFO @ Fri, 04 Aug 2017 15:08:52: #1 tag size = 50 INFO @ Fri, 04 Aug 2017 15:08:52: #1 total tags in treatment: 18013445 INFO @ Fri, 04 Aug 2017 15:08:52: #1 user defined the maximum tags... INFO @ Fri, 04 Aug 2017 15:08:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 04 Aug 2017 15:08:52: #1 tag size is determined as 50 bps INFO @ Fri, 04 Aug 2017 15:08:52: #1 tag size = 50 INFO @ Fri, 04 Aug 2017 15:08:52: #1 total tags in treatment: 18013445 INFO @ Fri, 04 Aug 2017 15:08:52: #1 user defined the maximum tags... INFO @ Fri, 04 Aug 2017 15:08:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 04 Aug 2017 15:08:52: #1 tags after filtering in treatment: 18013445 INFO @ Fri, 04 Aug 2017 15:08:52: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 04 Aug 2017 15:08:52: #1 finished! INFO @ Fri, 04 Aug 2017 15:08:52: #2 Build Peak Model... INFO @ Fri, 04 Aug 2017 15:08:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 04 Aug 2017 15:08:52: #1 tags after filtering in treatment: 18013445 INFO @ Fri, 04 Aug 2017 15:08:52: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 04 Aug 2017 15:08:52: #1 finished! INFO @ Fri, 04 Aug 2017 15:08:52: #2 Build Peak Model... INFO @ Fri, 04 Aug 2017 15:08:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 04 Aug 2017 15:08:53: #2 number of paired peaks: 13 WARNING @ Fri, 04 Aug 2017 15:08:53: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 04 Aug 2017 15:08:53: Process for pairing-model is terminated! cat: SRX2520650.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis INFO @ Fri, 04 Aug 2017 15:08:53: #2 number of paired peaks: 13 WARNING @ Fri, 04 Aug 2017 15:08:53: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 04 Aug 2017 15:08:53: Process for pairing-model is terminated! needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2520650.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2520650.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2520650.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling cat: SRX2520650.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2520650.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2520650.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2520650.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。