Job ID = 9158704 sra ファイルのダウンロード中... Completed: 511222K bytes transferred in 7 seconds (567758K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 28239481 spots for /home/okishinya/chipatlas/results/dm3/SRX2385060/SRR5064691.sra Written 28239481 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:43 28239481 reads; of these: 28239481 (100.00%) were unpaired; of these: 1184496 (4.19%) aligned 0 times 19340561 (68.49%) aligned exactly 1 time 7714424 (27.32%) aligned >1 times 95.81% overall alignment rate Time searching: 00:11:43 Overall time: 00:11:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6740695 / 27054985 = 0.2491 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 19:28:27: # Command line: callpeak -t SRX2385060.bam -f BAM -g dm -n SRX2385060.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2385060.05 # format = BAM # ChIP-seq file = ['SRX2385060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:28:27: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:28:27: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:28:27: # Command line: callpeak -t SRX2385060.bam -f BAM -g dm -n SRX2385060.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2385060.10 # format = BAM # ChIP-seq file = ['SRX2385060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:28:27: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:28:27: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:28:27: # Command line: callpeak -t SRX2385060.bam -f BAM -g dm -n SRX2385060.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2385060.20 # format = BAM # ChIP-seq file = ['SRX2385060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:28:27: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:28:27: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:28:33: 1000000 INFO @ Tue, 27 Jun 2017 19:28:33: 1000000 INFO @ Tue, 27 Jun 2017 19:28:33: 1000000 INFO @ Tue, 27 Jun 2017 19:28:40: 2000000 INFO @ Tue, 27 Jun 2017 19:28:40: 2000000 INFO @ Tue, 27 Jun 2017 19:28:40: 2000000 INFO @ Tue, 27 Jun 2017 19:28:46: 3000000 INFO @ Tue, 27 Jun 2017 19:28:47: 3000000 INFO @ Tue, 27 Jun 2017 19:28:47: 3000000 INFO @ Tue, 27 Jun 2017 19:28:52: 4000000 INFO @ Tue, 27 Jun 2017 19:28:53: 4000000 INFO @ Tue, 27 Jun 2017 19:28:53: 4000000 INFO @ Tue, 27 Jun 2017 19:28:58: 5000000 INFO @ Tue, 27 Jun 2017 19:28:59: 5000000 INFO @ Tue, 27 Jun 2017 19:29:00: 5000000 INFO @ Tue, 27 Jun 2017 19:29:04: 6000000 INFO @ Tue, 27 Jun 2017 19:29:05: 6000000 INFO @ Tue, 27 Jun 2017 19:29:06: 6000000 INFO @ Tue, 27 Jun 2017 19:29:10: 7000000 INFO @ Tue, 27 Jun 2017 19:29:12: 7000000 INFO @ Tue, 27 Jun 2017 19:29:12: 7000000 INFO @ Tue, 27 Jun 2017 19:29:16: 8000000 INFO @ Tue, 27 Jun 2017 19:29:18: 8000000 INFO @ Tue, 27 Jun 2017 19:29:19: 8000000 INFO @ Tue, 27 Jun 2017 19:29:22: 9000000 INFO @ Tue, 27 Jun 2017 19:29:25: 9000000 INFO @ Tue, 27 Jun 2017 19:29:26: 9000000 INFO @ Tue, 27 Jun 2017 19:29:28: 10000000 INFO @ Tue, 27 Jun 2017 19:29:31: 10000000 INFO @ Tue, 27 Jun 2017 19:29:32: 10000000 INFO @ Tue, 27 Jun 2017 19:29:34: 11000000 INFO @ Tue, 27 Jun 2017 19:29:38: 11000000 INFO @ Tue, 27 Jun 2017 19:29:39: 11000000 INFO @ Tue, 27 Jun 2017 19:29:40: 12000000 INFO @ Tue, 27 Jun 2017 19:29:44: 12000000 INFO @ Tue, 27 Jun 2017 19:29:45: 12000000 INFO @ Tue, 27 Jun 2017 19:29:46: 13000000 INFO @ Tue, 27 Jun 2017 19:29:51: 13000000 INFO @ Tue, 27 Jun 2017 19:29:52: 13000000 INFO @ Tue, 27 Jun 2017 19:29:52: 14000000 INFO @ Tue, 27 Jun 2017 19:29:58: 14000000 INFO @ Tue, 27 Jun 2017 19:29:59: 14000000 INFO @ Tue, 27 Jun 2017 19:29:59: 15000000 INFO @ Tue, 27 Jun 2017 19:30:05: 15000000 INFO @ Tue, 27 Jun 2017 19:30:07: 15000000 INFO @ Tue, 27 Jun 2017 19:30:07: 16000000 INFO @ Tue, 27 Jun 2017 19:30:13: 16000000 INFO @ Tue, 27 Jun 2017 19:30:15: 16000000 INFO @ Tue, 27 Jun 2017 19:30:15: 17000000 INFO @ Tue, 27 Jun 2017 19:30:21: 17000000 INFO @ Tue, 27 Jun 2017 19:30:22: 17000000 INFO @ Tue, 27 Jun 2017 19:30:22: 18000000 INFO @ Tue, 27 Jun 2017 19:30:28: 18000000 INFO @ Tue, 27 Jun 2017 19:30:30: 19000000 INFO @ Tue, 27 Jun 2017 19:30:30: 18000000 INFO @ Tue, 27 Jun 2017 19:30:36: 19000000 INFO @ Tue, 27 Jun 2017 19:30:37: 20000000 INFO @ Tue, 27 Jun 2017 19:30:38: 19000000 INFO @ Tue, 27 Jun 2017 19:30:40: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 19:30:40: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 19:30:40: #1 total tags in treatment: 20314290 INFO @ Tue, 27 Jun 2017 19:30:40: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:30:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:30:40: #1 tags after filtering in treatment: 20314290 INFO @ Tue, 27 Jun 2017 19:30:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:30:40: #1 finished! INFO @ Tue, 27 Jun 2017 19:30:40: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:30:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:30:42: #2 number of paired peaks: 41 WARNING @ Tue, 27 Jun 2017 19:30:42: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:30:42: Process for pairing-model is terminated! cat: SRX2385060.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2385060.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2385060.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2385060.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:30:44: 20000000 INFO @ Tue, 27 Jun 2017 19:30:45: 20000000 INFO @ Tue, 27 Jun 2017 19:30:46: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 19:30:46: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 19:30:46: #1 total tags in treatment: 20314290 INFO @ Tue, 27 Jun 2017 19:30:46: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:30:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:30:46: #1 tags after filtering in treatment: 20314290 INFO @ Tue, 27 Jun 2017 19:30:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:30:46: #1 finished! INFO @ Tue, 27 Jun 2017 19:30:46: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:30:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:30:47: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 19:30:47: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 19:30:47: #1 total tags in treatment: 20314290 INFO @ Tue, 27 Jun 2017 19:30:47: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:30:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:30:48: #2 number of paired peaks: 41 WARNING @ Tue, 27 Jun 2017 19:30:48: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:30:48: Process for pairing-model is terminated! cat: SRX2385060.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2385060.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2385060.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2385060.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:30:48: #1 tags after filtering in treatment: 20314290 INFO @ Tue, 27 Jun 2017 19:30:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:30:48: #1 finished! INFO @ Tue, 27 Jun 2017 19:30:48: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:30:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:30:49: #2 number of paired peaks: 41 WARNING @ Tue, 27 Jun 2017 19:30:49: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:30:49: Process for pairing-model is terminated! cat: SRX2385060.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2385060.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2385060.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2385060.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。