Job ID = 9158703 sra ファイルのダウンロード中... Completed: 369524K bytes transferred in 6 seconds (493003K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 20501669 spots for /home/okishinya/chipatlas/results/dm3/SRX2385059/SRR5064690.sra Written 20501669 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:01 20501669 reads; of these: 20501669 (100.00%) were unpaired; of these: 637161 (3.11%) aligned 0 times 14263808 (69.57%) aligned exactly 1 time 5600700 (27.32%) aligned >1 times 96.89% overall alignment rate Time searching: 00:09:01 Overall time: 00:09:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2746151 / 19864508 = 0.1382 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 19:23:09: # Command line: callpeak -t SRX2385059.bam -f BAM -g dm -n SRX2385059.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2385059.20 # format = BAM # ChIP-seq file = ['SRX2385059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:23:09: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:23:09: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:23:09: # Command line: callpeak -t SRX2385059.bam -f BAM -g dm -n SRX2385059.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2385059.05 # format = BAM # ChIP-seq file = ['SRX2385059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:23:09: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:23:09: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:23:09: # Command line: callpeak -t SRX2385059.bam -f BAM -g dm -n SRX2385059.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2385059.10 # format = BAM # ChIP-seq file = ['SRX2385059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:23:09: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:23:09: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:23:16: 1000000 INFO @ Tue, 27 Jun 2017 19:23:16: 1000000 INFO @ Tue, 27 Jun 2017 19:23:16: 1000000 INFO @ Tue, 27 Jun 2017 19:23:22: 2000000 INFO @ Tue, 27 Jun 2017 19:23:24: 2000000 INFO @ Tue, 27 Jun 2017 19:23:24: 2000000 INFO @ Tue, 27 Jun 2017 19:23:29: 3000000 INFO @ Tue, 27 Jun 2017 19:23:31: 3000000 INFO @ Tue, 27 Jun 2017 19:23:31: 3000000 INFO @ Tue, 27 Jun 2017 19:23:36: 4000000 INFO @ Tue, 27 Jun 2017 19:23:39: 4000000 INFO @ Tue, 27 Jun 2017 19:23:39: 4000000 INFO @ Tue, 27 Jun 2017 19:23:42: 5000000 INFO @ Tue, 27 Jun 2017 19:23:46: 5000000 INFO @ Tue, 27 Jun 2017 19:23:46: 5000000 INFO @ Tue, 27 Jun 2017 19:23:49: 6000000 INFO @ Tue, 27 Jun 2017 19:23:54: 6000000 INFO @ Tue, 27 Jun 2017 19:23:54: 6000000 INFO @ Tue, 27 Jun 2017 19:23:55: 7000000 INFO @ Tue, 27 Jun 2017 19:24:01: 7000000 INFO @ Tue, 27 Jun 2017 19:24:01: 7000000 INFO @ Tue, 27 Jun 2017 19:24:02: 8000000 INFO @ Tue, 27 Jun 2017 19:24:08: 8000000 INFO @ Tue, 27 Jun 2017 19:24:09: 8000000 INFO @ Tue, 27 Jun 2017 19:24:09: 9000000 INFO @ Tue, 27 Jun 2017 19:24:15: 9000000 INFO @ Tue, 27 Jun 2017 19:24:17: 10000000 INFO @ Tue, 27 Jun 2017 19:24:17: 9000000 INFO @ Tue, 27 Jun 2017 19:24:22: 10000000 INFO @ Tue, 27 Jun 2017 19:24:25: 11000000 INFO @ Tue, 27 Jun 2017 19:24:26: 10000000 INFO @ Tue, 27 Jun 2017 19:24:30: 11000000 INFO @ Tue, 27 Jun 2017 19:24:32: 12000000 INFO @ Tue, 27 Jun 2017 19:24:34: 11000000 INFO @ Tue, 27 Jun 2017 19:24:37: 12000000 INFO @ Tue, 27 Jun 2017 19:24:39: 13000000 INFO @ Tue, 27 Jun 2017 19:24:42: 12000000 INFO @ Tue, 27 Jun 2017 19:24:44: 13000000 INFO @ Tue, 27 Jun 2017 19:24:47: 14000000 INFO @ Tue, 27 Jun 2017 19:24:51: 13000000 INFO @ Tue, 27 Jun 2017 19:24:52: 14000000 INFO @ Tue, 27 Jun 2017 19:24:55: 15000000 INFO @ Tue, 27 Jun 2017 19:24:59: 14000000 INFO @ Tue, 27 Jun 2017 19:24:59: 15000000 INFO @ Tue, 27 Jun 2017 19:25:02: 16000000 INFO @ Tue, 27 Jun 2017 19:25:07: 15000000 INFO @ Tue, 27 Jun 2017 19:25:07: 16000000 INFO @ Tue, 27 Jun 2017 19:25:09: 17000000 INFO @ Tue, 27 Jun 2017 19:25:09: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 19:25:09: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 19:25:09: #1 total tags in treatment: 17118357 INFO @ Tue, 27 Jun 2017 19:25:09: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:25:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:25:10: #1 tags after filtering in treatment: 17118357 INFO @ Tue, 27 Jun 2017 19:25:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:25:10: #1 finished! INFO @ Tue, 27 Jun 2017 19:25:10: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:25:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:25:11: #2 number of paired peaks: 63 WARNING @ Tue, 27 Jun 2017 19:25:11: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:25:11: Process for pairing-model is terminated! cat: SRX2385059.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2385059.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2385059.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2385059.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:25:15: 16000000 INFO @ Tue, 27 Jun 2017 19:25:15: 17000000 INFO @ Tue, 27 Jun 2017 19:25:16: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 19:25:16: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 19:25:16: #1 total tags in treatment: 17118357 INFO @ Tue, 27 Jun 2017 19:25:16: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:25:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:25:16: #1 tags after filtering in treatment: 17118357 INFO @ Tue, 27 Jun 2017 19:25:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:25:16: #1 finished! INFO @ Tue, 27 Jun 2017 19:25:16: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:25:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:25:17: #2 number of paired peaks: 63 WARNING @ Tue, 27 Jun 2017 19:25:17: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:25:17: Process for pairing-model is terminated! cat: SRX2385059.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2385059.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2385059.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2385059.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:25:21: 17000000 INFO @ Tue, 27 Jun 2017 19:25:22: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 19:25:22: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 19:25:22: #1 total tags in treatment: 17118357 INFO @ Tue, 27 Jun 2017 19:25:22: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:25:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:25:23: #1 tags after filtering in treatment: 17118357 INFO @ Tue, 27 Jun 2017 19:25:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:25:23: #1 finished! INFO @ Tue, 27 Jun 2017 19:25:23: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:25:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:25:24: #2 number of paired peaks: 63 WARNING @ Tue, 27 Jun 2017 19:25:24: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:25:24: Process for pairing-model is terminated! cat: SRX2385059.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2385059.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2385059.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2385059.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。