Job ID = 11171158 sra ファイルのダウンロード中... Completed: 3369435K bytes transferred in 69 seconds (399271K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 118861330 spots for /home/okishinya/chipatlas/results/dm3/SRX2325664/SRR4896352.sra Written 118861330 spots for /home/okishinya/chipatlas/results/dm3/SRX2325664/SRR4896352.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:04:22 118861330 reads; of these: 118861330 (100.00%) were unpaired; of these: 8429672 (7.09%) aligned 0 times 87656800 (73.75%) aligned exactly 1 time 22774858 (19.16%) aligned >1 times 92.91% overall alignment rate Time searching: 01:04:22 Overall time: 01:04:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 58158029 / 110431658 = 0.5266 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 13:55:21: # Command line: callpeak -t SRX2325664.bam -f BAM -g dm -n SRX2325664.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2325664.05 # format = BAM # ChIP-seq file = ['SRX2325664.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:55:21: # Command line: callpeak -t SRX2325664.bam -f BAM -g dm -n SRX2325664.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2325664.10 # format = BAM # ChIP-seq file = ['SRX2325664.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:55:21: # Command line: callpeak -t SRX2325664.bam -f BAM -g dm -n SRX2325664.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2325664.20 # format = BAM # ChIP-seq file = ['SRX2325664.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 13:55:21: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:55:21: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:55:21: #1 read tag files... INFO @ Sat, 08 Sep 2018 13:55:21: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:55:21: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:55:21: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 13:55:30: 1000000 INFO @ Sat, 08 Sep 2018 13:55:30: 1000000 INFO @ Sat, 08 Sep 2018 13:55:30: 1000000 INFO @ Sat, 08 Sep 2018 13:55:38: 2000000 INFO @ Sat, 08 Sep 2018 13:55:38: 2000000 INFO @ Sat, 08 Sep 2018 13:55:38: 2000000 INFO @ Sat, 08 Sep 2018 13:55:47: 3000000 INFO @ Sat, 08 Sep 2018 13:55:47: 3000000 INFO @ Sat, 08 Sep 2018 13:55:47: 3000000 INFO @ Sat, 08 Sep 2018 13:55:56: 4000000 INFO @ Sat, 08 Sep 2018 13:55:56: 4000000 INFO @ Sat, 08 Sep 2018 13:55:56: 4000000 INFO @ Sat, 08 Sep 2018 13:56:04: 5000000 INFO @ Sat, 08 Sep 2018 13:56:04: 5000000 INFO @ Sat, 08 Sep 2018 13:56:04: 5000000 INFO @ Sat, 08 Sep 2018 13:56:12: 6000000 INFO @ Sat, 08 Sep 2018 13:56:12: 6000000 INFO @ Sat, 08 Sep 2018 13:56:12: 6000000 INFO @ Sat, 08 Sep 2018 13:56:19: 7000000 INFO @ Sat, 08 Sep 2018 13:56:19: 7000000 INFO @ Sat, 08 Sep 2018 13:56:20: 7000000 INFO @ Sat, 08 Sep 2018 13:56:27: 8000000 INFO @ Sat, 08 Sep 2018 13:56:27: 8000000 INFO @ Sat, 08 Sep 2018 13:56:28: 8000000 INFO @ Sat, 08 Sep 2018 13:56:35: 9000000 INFO @ Sat, 08 Sep 2018 13:56:35: 9000000 INFO @ Sat, 08 Sep 2018 13:56:36: 9000000 INFO @ Sat, 08 Sep 2018 13:56:43: 10000000 INFO @ Sat, 08 Sep 2018 13:56:43: 10000000 INFO @ Sat, 08 Sep 2018 13:56:44: 10000000 INFO @ Sat, 08 Sep 2018 13:56:51: 11000000 INFO @ Sat, 08 Sep 2018 13:56:51: 11000000 INFO @ Sat, 08 Sep 2018 13:56:52: 11000000 INFO @ Sat, 08 Sep 2018 13:57:00: 12000000 INFO @ Sat, 08 Sep 2018 13:57:00: 12000000 INFO @ Sat, 08 Sep 2018 13:57:00: 12000000 INFO @ Sat, 08 Sep 2018 13:57:08: 13000000 INFO @ Sat, 08 Sep 2018 13:57:08: 13000000 INFO @ Sat, 08 Sep 2018 13:57:08: 13000000 INFO @ Sat, 08 Sep 2018 13:57:16: 14000000 INFO @ Sat, 08 Sep 2018 13:57:16: 14000000 INFO @ Sat, 08 Sep 2018 13:57:16: 14000000 INFO @ Sat, 08 Sep 2018 13:57:24: 15000000 INFO @ Sat, 08 Sep 2018 13:57:24: 15000000 INFO @ Sat, 08 Sep 2018 13:57:25: 15000000 INFO @ Sat, 08 Sep 2018 13:57:32: 16000000 INFO @ Sat, 08 Sep 2018 13:57:32: 16000000 INFO @ Sat, 08 Sep 2018 13:57:33: 16000000 INFO @ Sat, 08 Sep 2018 13:57:40: 17000000 INFO @ Sat, 08 Sep 2018 13:57:40: 17000000 INFO @ Sat, 08 Sep 2018 13:57:41: 17000000 INFO @ Sat, 08 Sep 2018 13:57:48: 18000000 INFO @ Sat, 08 Sep 2018 13:57:48: 18000000 INFO @ Sat, 08 Sep 2018 13:57:49: 18000000 INFO @ Sat, 08 Sep 2018 13:57:56: 19000000 INFO @ Sat, 08 Sep 2018 13:57:56: 19000000 INFO @ Sat, 08 Sep 2018 13:57:57: 19000000 INFO @ Sat, 08 Sep 2018 13:58:05: 20000000 INFO @ Sat, 08 Sep 2018 13:58:05: 20000000 INFO @ Sat, 08 Sep 2018 13:58:05: 20000000 INFO @ Sat, 08 Sep 2018 13:58:13: 21000000 INFO @ Sat, 08 Sep 2018 13:58:13: 21000000 INFO @ Sat, 08 Sep 2018 13:58:13: 21000000 INFO @ Sat, 08 Sep 2018 13:58:20: 22000000 INFO @ Sat, 08 Sep 2018 13:58:20: 22000000 INFO @ Sat, 08 Sep 2018 13:58:22: 22000000 INFO @ Sat, 08 Sep 2018 13:58:28: 23000000 INFO @ Sat, 08 Sep 2018 13:58:28: 23000000 INFO @ Sat, 08 Sep 2018 13:58:31: 23000000 INFO @ Sat, 08 Sep 2018 13:58:36: 24000000 INFO @ Sat, 08 Sep 2018 13:58:36: 24000000 INFO @ Sat, 08 Sep 2018 13:58:40: 24000000 INFO @ Sat, 08 Sep 2018 13:58:44: 25000000 INFO @ Sat, 08 Sep 2018 13:58:44: 25000000 INFO @ Sat, 08 Sep 2018 13:58:48: 25000000 INFO @ Sat, 08 Sep 2018 13:58:51: 26000000 INFO @ Sat, 08 Sep 2018 13:58:51: 26000000 INFO @ Sat, 08 Sep 2018 13:58:57: 26000000 INFO @ Sat, 08 Sep 2018 13:58:59: 27000000 INFO @ Sat, 08 Sep 2018 13:58:59: 27000000 INFO @ Sat, 08 Sep 2018 13:59:06: 27000000 INFO @ Sat, 08 Sep 2018 13:59:07: 28000000 INFO @ Sat, 08 Sep 2018 13:59:08: 28000000 INFO @ Sat, 08 Sep 2018 13:59:14: 29000000 INFO @ Sat, 08 Sep 2018 13:59:16: 28000000 INFO @ Sat, 08 Sep 2018 13:59:18: 29000000 INFO @ Sat, 08 Sep 2018 13:59:22: 30000000 INFO @ Sat, 08 Sep 2018 13:59:26: 29000000 INFO @ Sat, 08 Sep 2018 13:59:28: 30000000 INFO @ Sat, 08 Sep 2018 13:59:29: 31000000 INFO @ Sat, 08 Sep 2018 13:59:36: 30000000 INFO @ Sat, 08 Sep 2018 13:59:37: 32000000 INFO @ Sat, 08 Sep 2018 13:59:38: 31000000 INFO @ Sat, 08 Sep 2018 13:59:44: 33000000 INFO @ Sat, 08 Sep 2018 13:59:45: 31000000 INFO @ Sat, 08 Sep 2018 13:59:47: 32000000 INFO @ Sat, 08 Sep 2018 13:59:52: 34000000 INFO @ Sat, 08 Sep 2018 13:59:55: 32000000 INFO @ Sat, 08 Sep 2018 13:59:57: 33000000 INFO @ Sat, 08 Sep 2018 14:00:00: 35000000 INFO @ Sat, 08 Sep 2018 14:00:05: 33000000 INFO @ Sat, 08 Sep 2018 14:00:07: 34000000 INFO @ Sat, 08 Sep 2018 14:00:07: 36000000 INFO @ Sat, 08 Sep 2018 14:00:15: 34000000 INFO @ Sat, 08 Sep 2018 14:00:15: 37000000 INFO @ Sat, 08 Sep 2018 14:00:17: 35000000 INFO @ Sat, 08 Sep 2018 14:00:23: 38000000 INFO @ Sat, 08 Sep 2018 14:00:25: 35000000 INFO @ Sat, 08 Sep 2018 14:00:27: 36000000 INFO @ Sat, 08 Sep 2018 14:00:30: 39000000 INFO @ Sat, 08 Sep 2018 14:00:34: 36000000 INFO @ Sat, 08 Sep 2018 14:00:37: 37000000 INFO @ Sat, 08 Sep 2018 14:00:38: 40000000 INFO @ Sat, 08 Sep 2018 14:00:44: 37000000 INFO @ Sat, 08 Sep 2018 14:00:46: 38000000 INFO @ Sat, 08 Sep 2018 14:00:46: 41000000 INFO @ Sat, 08 Sep 2018 14:00:53: 38000000 INFO @ Sat, 08 Sep 2018 14:00:54: 42000000 INFO @ Sat, 08 Sep 2018 14:00:55: 39000000 INFO @ Sat, 08 Sep 2018 14:01:02: 43000000 INFO @ Sat, 08 Sep 2018 14:01:02: 39000000 INFO @ Sat, 08 Sep 2018 14:01:05: 40000000 INFO @ Sat, 08 Sep 2018 14:01:10: 44000000 INFO @ Sat, 08 Sep 2018 14:01:12: 40000000 INFO @ Sat, 08 Sep 2018 14:01:14: 41000000 INFO @ Sat, 08 Sep 2018 14:01:18: 45000000 INFO @ Sat, 08 Sep 2018 14:01:21: 41000000 INFO @ Sat, 08 Sep 2018 14:01:24: 42000000 INFO @ Sat, 08 Sep 2018 14:01:26: 46000000 INFO @ Sat, 08 Sep 2018 14:01:30: 42000000 INFO @ Sat, 08 Sep 2018 14:01:33: 43000000 INFO @ Sat, 08 Sep 2018 14:01:34: 47000000 INFO @ Sat, 08 Sep 2018 14:01:40: 43000000 INFO @ Sat, 08 Sep 2018 14:01:42: 48000000 INFO @ Sat, 08 Sep 2018 14:01:42: 44000000 INFO @ Sat, 08 Sep 2018 14:01:49: 44000000 INFO @ Sat, 08 Sep 2018 14:01:50: 49000000 INFO @ Sat, 08 Sep 2018 14:01:52: 45000000 INFO @ Sat, 08 Sep 2018 14:01:58: 50000000 INFO @ Sat, 08 Sep 2018 14:01:58: 45000000 INFO @ Sat, 08 Sep 2018 14:02:01: 46000000 INFO @ Sat, 08 Sep 2018 14:02:06: 51000000 INFO @ Sat, 08 Sep 2018 14:02:08: 46000000 INFO @ Sat, 08 Sep 2018 14:02:11: 47000000 INFO @ Sat, 08 Sep 2018 14:02:14: 52000000 INFO @ Sat, 08 Sep 2018 14:02:17: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 14:02:17: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 14:02:17: #1 total tags in treatment: 52273629 INFO @ Sat, 08 Sep 2018 14:02:17: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 14:02:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 14:02:17: 47000000 INFO @ Sat, 08 Sep 2018 14:02:18: #1 tags after filtering in treatment: 52273629 INFO @ Sat, 08 Sep 2018 14:02:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 14:02:18: #1 finished! INFO @ Sat, 08 Sep 2018 14:02:18: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 14:02:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 14:02:21: 48000000 INFO @ Sat, 08 Sep 2018 14:02:21: #2 number of paired peaks: 0 WARNING @ Sat, 08 Sep 2018 14:02:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 14:02:21: Process for pairing-model is terminated! cat: SRX2325664.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325664.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325664.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325664.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 14:02:27: 48000000 INFO @ Sat, 08 Sep 2018 14:02:30: 49000000 INFO @ Sat, 08 Sep 2018 14:02:36: 49000000 INFO @ Sat, 08 Sep 2018 14:02:40: 50000000 INFO @ Sat, 08 Sep 2018 14:02:46: 50000000 INFO @ Sat, 08 Sep 2018 14:02:49: 51000000 INFO @ Sat, 08 Sep 2018 14:02:55: 51000000 INFO @ Sat, 08 Sep 2018 14:02:58: 52000000 INFO @ Sat, 08 Sep 2018 14:03:01: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 14:03:01: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 14:03:01: #1 total tags in treatment: 52273629 INFO @ Sat, 08 Sep 2018 14:03:01: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 14:03:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 14:03:03: #1 tags after filtering in treatment: 52273629 INFO @ Sat, 08 Sep 2018 14:03:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 14:03:03: #1 finished! INFO @ Sat, 08 Sep 2018 14:03:03: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 14:03:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 14:03:04: 52000000 INFO @ Sat, 08 Sep 2018 14:03:06: #2 number of paired peaks: 0 WARNING @ Sat, 08 Sep 2018 14:03:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 14:03:06: Process for pairing-model is terminated! cat: SRX2325664.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Sat, 08 Sep 2018 14:03:06: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 14:03:06: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 14:03:06: #1 total tags in treatment: 52273629 INFO @ Sat, 08 Sep 2018 14:03:06: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 14:03:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (0 chroms): 162 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325664.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325664.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325664.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 14:03:07: #1 tags after filtering in treatment: 52273629 INFO @ Sat, 08 Sep 2018 14:03:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 14:03:07: #1 finished! INFO @ Sat, 08 Sep 2018 14:03:07: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 14:03:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 14:03:11: #2 number of paired peaks: 0 WARNING @ Sat, 08 Sep 2018 14:03:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 14:03:11: Process for pairing-model is terminated! cat: SRX2325664.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325664.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325664.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325664.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。