Job ID = 11171154 sra ファイルのダウンロード中... Completed: 1677916K bytes transferred in 31 seconds (436213K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 65089711 spots for /home/okishinya/chipatlas/results/dm3/SRX2325660/SRR4896348.sra Written 65089711 spots for /home/okishinya/chipatlas/results/dm3/SRX2325660/SRR4896348.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:30:58 65089711 reads; of these: 65089711 (100.00%) were unpaired; of these: 8389148 (12.89%) aligned 0 times 45636054 (70.11%) aligned exactly 1 time 11064509 (17.00%) aligned >1 times 87.11% overall alignment rate Time searching: 00:30:58 Overall time: 00:30:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 18716040 / 56700563 = 0.3301 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 12:58:17: # Command line: callpeak -t SRX2325660.bam -f BAM -g dm -n SRX2325660.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2325660.05 # format = BAM # ChIP-seq file = ['SRX2325660.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:58:17: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:58:17: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:58:17: # Command line: callpeak -t SRX2325660.bam -f BAM -g dm -n SRX2325660.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2325660.10 # format = BAM # ChIP-seq file = ['SRX2325660.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:58:17: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:58:17: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:58:17: # Command line: callpeak -t SRX2325660.bam -f BAM -g dm -n SRX2325660.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2325660.20 # format = BAM # ChIP-seq file = ['SRX2325660.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:58:17: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:58:17: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:58:25: 1000000 INFO @ Sat, 08 Sep 2018 12:58:25: 1000000 INFO @ Sat, 08 Sep 2018 12:58:25: 1000000 INFO @ Sat, 08 Sep 2018 12:58:32: 2000000 INFO @ Sat, 08 Sep 2018 12:58:33: 2000000 INFO @ Sat, 08 Sep 2018 12:58:33: 2000000 INFO @ Sat, 08 Sep 2018 12:58:39: 3000000 INFO @ Sat, 08 Sep 2018 12:58:41: 3000000 INFO @ Sat, 08 Sep 2018 12:58:41: 3000000 INFO @ Sat, 08 Sep 2018 12:58:47: 4000000 INFO @ Sat, 08 Sep 2018 12:58:49: 4000000 INFO @ Sat, 08 Sep 2018 12:58:49: 4000000 INFO @ Sat, 08 Sep 2018 12:58:54: 5000000 INFO @ Sat, 08 Sep 2018 12:58:57: 5000000 INFO @ Sat, 08 Sep 2018 12:58:57: 5000000 INFO @ Sat, 08 Sep 2018 12:59:01: 6000000 INFO @ Sat, 08 Sep 2018 12:59:05: 6000000 INFO @ Sat, 08 Sep 2018 12:59:05: 6000000 INFO @ Sat, 08 Sep 2018 12:59:08: 7000000 INFO @ Sat, 08 Sep 2018 12:59:13: 7000000 INFO @ Sat, 08 Sep 2018 12:59:13: 7000000 INFO @ Sat, 08 Sep 2018 12:59:16: 8000000 INFO @ Sat, 08 Sep 2018 12:59:20: 8000000 INFO @ Sat, 08 Sep 2018 12:59:20: 8000000 INFO @ Sat, 08 Sep 2018 12:59:23: 9000000 INFO @ Sat, 08 Sep 2018 12:59:28: 9000000 INFO @ Sat, 08 Sep 2018 12:59:28: 9000000 INFO @ Sat, 08 Sep 2018 12:59:30: 10000000 INFO @ Sat, 08 Sep 2018 12:59:36: 10000000 INFO @ Sat, 08 Sep 2018 12:59:36: 10000000 INFO @ Sat, 08 Sep 2018 12:59:37: 11000000 INFO @ Sat, 08 Sep 2018 12:59:44: 11000000 INFO @ Sat, 08 Sep 2018 12:59:44: 11000000 INFO @ Sat, 08 Sep 2018 12:59:45: 12000000 INFO @ Sat, 08 Sep 2018 12:59:52: 13000000 INFO @ Sat, 08 Sep 2018 12:59:52: 12000000 INFO @ Sat, 08 Sep 2018 12:59:52: 12000000 INFO @ Sat, 08 Sep 2018 12:59:59: 14000000 INFO @ Sat, 08 Sep 2018 13:00:00: 13000000 INFO @ Sat, 08 Sep 2018 13:00:00: 13000000 INFO @ Sat, 08 Sep 2018 13:00:06: 15000000 INFO @ Sat, 08 Sep 2018 13:00:08: 14000000 INFO @ Sat, 08 Sep 2018 13:00:08: 14000000 INFO @ Sat, 08 Sep 2018 13:00:14: 16000000 INFO @ Sat, 08 Sep 2018 13:00:15: 15000000 INFO @ Sat, 08 Sep 2018 13:00:15: 15000000 INFO @ Sat, 08 Sep 2018 13:00:21: 17000000 INFO @ Sat, 08 Sep 2018 13:00:23: 16000000 INFO @ Sat, 08 Sep 2018 13:00:23: 16000000 INFO @ Sat, 08 Sep 2018 13:00:28: 18000000 INFO @ Sat, 08 Sep 2018 13:00:31: 17000000 INFO @ Sat, 08 Sep 2018 13:00:31: 17000000 INFO @ Sat, 08 Sep 2018 13:00:35: 19000000 INFO @ Sat, 08 Sep 2018 13:00:39: 18000000 INFO @ Sat, 08 Sep 2018 13:00:39: 18000000 INFO @ Sat, 08 Sep 2018 13:00:43: 20000000 INFO @ Sat, 08 Sep 2018 13:00:47: 19000000 INFO @ Sat, 08 Sep 2018 13:00:47: 19000000 INFO @ Sat, 08 Sep 2018 13:00:50: 21000000 INFO @ Sat, 08 Sep 2018 13:00:55: 20000000 INFO @ Sat, 08 Sep 2018 13:00:55: 20000000 INFO @ Sat, 08 Sep 2018 13:00:57: 22000000 INFO @ Sat, 08 Sep 2018 13:01:03: 21000000 INFO @ Sat, 08 Sep 2018 13:01:03: 21000000 INFO @ Sat, 08 Sep 2018 13:01:04: 23000000 INFO @ Sat, 08 Sep 2018 13:01:11: 22000000 INFO @ Sat, 08 Sep 2018 13:01:11: 22000000 INFO @ Sat, 08 Sep 2018 13:01:12: 24000000 INFO @ Sat, 08 Sep 2018 13:01:18: 23000000 INFO @ Sat, 08 Sep 2018 13:01:18: 23000000 INFO @ Sat, 08 Sep 2018 13:01:19: 25000000 INFO @ Sat, 08 Sep 2018 13:01:26: 26000000 INFO @ Sat, 08 Sep 2018 13:01:26: 24000000 INFO @ Sat, 08 Sep 2018 13:01:26: 24000000 INFO @ Sat, 08 Sep 2018 13:01:33: 27000000 INFO @ Sat, 08 Sep 2018 13:01:34: 25000000 INFO @ Sat, 08 Sep 2018 13:01:34: 25000000 INFO @ Sat, 08 Sep 2018 13:01:40: 28000000 INFO @ Sat, 08 Sep 2018 13:01:42: 26000000 INFO @ Sat, 08 Sep 2018 13:01:42: 26000000 INFO @ Sat, 08 Sep 2018 13:01:48: 29000000 INFO @ Sat, 08 Sep 2018 13:01:50: 27000000 INFO @ Sat, 08 Sep 2018 13:01:50: 27000000 INFO @ Sat, 08 Sep 2018 13:01:55: 30000000 INFO @ Sat, 08 Sep 2018 13:01:58: 28000000 INFO @ Sat, 08 Sep 2018 13:01:58: 28000000 INFO @ Sat, 08 Sep 2018 13:02:02: 31000000 INFO @ Sat, 08 Sep 2018 13:02:05: 29000000 INFO @ Sat, 08 Sep 2018 13:02:05: 29000000 INFO @ Sat, 08 Sep 2018 13:02:10: 32000000 INFO @ Sat, 08 Sep 2018 13:02:13: 30000000 INFO @ Sat, 08 Sep 2018 13:02:13: 30000000 INFO @ Sat, 08 Sep 2018 13:02:17: 33000000 INFO @ Sat, 08 Sep 2018 13:02:21: 31000000 INFO @ Sat, 08 Sep 2018 13:02:22: 31000000 INFO @ Sat, 08 Sep 2018 13:02:25: 34000000 INFO @ Sat, 08 Sep 2018 13:02:28: 32000000 INFO @ Sat, 08 Sep 2018 13:02:30: 32000000 INFO @ Sat, 08 Sep 2018 13:02:34: 35000000 INFO @ Sat, 08 Sep 2018 13:02:36: 33000000 INFO @ Sat, 08 Sep 2018 13:02:39: 33000000 INFO @ Sat, 08 Sep 2018 13:02:42: 36000000 INFO @ Sat, 08 Sep 2018 13:02:43: 34000000 INFO @ Sat, 08 Sep 2018 13:02:48: 34000000 INFO @ Sat, 08 Sep 2018 13:02:50: 37000000 INFO @ Sat, 08 Sep 2018 13:02:50: 35000000 INFO @ Sat, 08 Sep 2018 13:02:56: 35000000 INFO @ Sat, 08 Sep 2018 13:02:58: 36000000 INFO @ Sat, 08 Sep 2018 13:02:58: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 13:02:58: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 13:02:58: #1 total tags in treatment: 37984523 INFO @ Sat, 08 Sep 2018 13:02:58: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:02:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:02:59: #1 tags after filtering in treatment: 37984523 INFO @ Sat, 08 Sep 2018 13:02:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:02:59: #1 finished! INFO @ Sat, 08 Sep 2018 13:02:59: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:02:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:03:01: #2 number of paired peaks: 1 WARNING @ Sat, 08 Sep 2018 13:03:01: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:03:01: Process for pairing-model is terminated! cat: SRX2325660.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325660.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325660.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325660.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:03:04: 36000000 INFO @ Sat, 08 Sep 2018 13:03:05: 37000000 INFO @ Sat, 08 Sep 2018 13:03:11: 37000000 INFO @ Sat, 08 Sep 2018 13:03:12: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 13:03:12: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 13:03:12: #1 total tags in treatment: 37984523 INFO @ Sat, 08 Sep 2018 13:03:12: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:03:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:03:13: #1 tags after filtering in treatment: 37984523 INFO @ Sat, 08 Sep 2018 13:03:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:03:13: #1 finished! INFO @ Sat, 08 Sep 2018 13:03:13: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:03:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:03:15: #2 number of paired peaks: 1 WARNING @ Sat, 08 Sep 2018 13:03:15: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:03:15: Process for pairing-model is terminated! cat: SRX2325660.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325660.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325660.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325660.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 13:03:19: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 13:03:19: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 13:03:19: #1 total tags in treatment: 37984523 INFO @ Sat, 08 Sep 2018 13:03:19: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 13:03:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 13:03:19: #1 tags after filtering in treatment: 37984523 INFO @ Sat, 08 Sep 2018 13:03:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 13:03:19: #1 finished! INFO @ Sat, 08 Sep 2018 13:03:19: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 13:03:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 13:03:22: #2 number of paired peaks: 1 WARNING @ Sat, 08 Sep 2018 13:03:22: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 13:03:22: Process for pairing-model is terminated! cat: SRX2325660.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325660.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325660.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325660.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。