Job ID = 11171151 sra ファイルのダウンロード中... Completed: 1493729K bytes transferred in 16 seconds (733530K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 57994697 spots for /home/okishinya/chipatlas/results/dm3/SRX2325657/SRR4896345.sra Written 57994697 spots for /home/okishinya/chipatlas/results/dm3/SRX2325657/SRR4896345.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:58 57994697 reads; of these: 57994697 (100.00%) were unpaired; of these: 2693611 (4.64%) aligned 0 times 44217078 (76.24%) aligned exactly 1 time 11084008 (19.11%) aligned >1 times 95.36% overall alignment rate Time searching: 00:28:58 Overall time: 00:28:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 11188925 / 55301086 = 0.2023 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 12:54:16: # Command line: callpeak -t SRX2325657.bam -f BAM -g dm -n SRX2325657.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2325657.20 # format = BAM # ChIP-seq file = ['SRX2325657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:54:16: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:54:16: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:54:16: # Command line: callpeak -t SRX2325657.bam -f BAM -g dm -n SRX2325657.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2325657.05 # format = BAM # ChIP-seq file = ['SRX2325657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:54:16: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:54:16: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:54:16: # Command line: callpeak -t SRX2325657.bam -f BAM -g dm -n SRX2325657.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2325657.10 # format = BAM # ChIP-seq file = ['SRX2325657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:54:16: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:54:16: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:54:23: 1000000 INFO @ Sat, 08 Sep 2018 12:54:23: 1000000 INFO @ Sat, 08 Sep 2018 12:54:23: 1000000 INFO @ Sat, 08 Sep 2018 12:54:30: 2000000 INFO @ Sat, 08 Sep 2018 12:54:30: 2000000 INFO @ Sat, 08 Sep 2018 12:54:31: 2000000 INFO @ Sat, 08 Sep 2018 12:54:38: 3000000 INFO @ Sat, 08 Sep 2018 12:54:38: 3000000 INFO @ Sat, 08 Sep 2018 12:54:39: 3000000 INFO @ Sat, 08 Sep 2018 12:54:45: 4000000 INFO @ Sat, 08 Sep 2018 12:54:45: 4000000 INFO @ Sat, 08 Sep 2018 12:54:47: 4000000 INFO @ Sat, 08 Sep 2018 12:54:52: 5000000 INFO @ Sat, 08 Sep 2018 12:54:53: 5000000 INFO @ Sat, 08 Sep 2018 12:54:55: 5000000 INFO @ Sat, 08 Sep 2018 12:55:00: 6000000 INFO @ Sat, 08 Sep 2018 12:55:00: 6000000 INFO @ Sat, 08 Sep 2018 12:55:03: 6000000 INFO @ Sat, 08 Sep 2018 12:55:07: 7000000 INFO @ Sat, 08 Sep 2018 12:55:08: 7000000 INFO @ Sat, 08 Sep 2018 12:55:11: 7000000 INFO @ Sat, 08 Sep 2018 12:55:14: 8000000 INFO @ Sat, 08 Sep 2018 12:55:15: 8000000 INFO @ Sat, 08 Sep 2018 12:55:19: 8000000 INFO @ Sat, 08 Sep 2018 12:55:21: 9000000 INFO @ Sat, 08 Sep 2018 12:55:22: 9000000 INFO @ Sat, 08 Sep 2018 12:55:27: 9000000 INFO @ Sat, 08 Sep 2018 12:55:29: 10000000 INFO @ Sat, 08 Sep 2018 12:55:29: 10000000 INFO @ Sat, 08 Sep 2018 12:55:34: 10000000 INFO @ Sat, 08 Sep 2018 12:55:36: 11000000 INFO @ Sat, 08 Sep 2018 12:55:36: 11000000 INFO @ Sat, 08 Sep 2018 12:55:42: 11000000 INFO @ Sat, 08 Sep 2018 12:55:43: 12000000 INFO @ Sat, 08 Sep 2018 12:55:44: 12000000 INFO @ Sat, 08 Sep 2018 12:55:49: 12000000 INFO @ Sat, 08 Sep 2018 12:55:50: 13000000 INFO @ Sat, 08 Sep 2018 12:55:51: 13000000 INFO @ Sat, 08 Sep 2018 12:55:56: 13000000 INFO @ Sat, 08 Sep 2018 12:55:57: 14000000 INFO @ Sat, 08 Sep 2018 12:55:59: 14000000 INFO @ Sat, 08 Sep 2018 12:56:04: 15000000 INFO @ Sat, 08 Sep 2018 12:56:04: 14000000 INFO @ Sat, 08 Sep 2018 12:56:06: 15000000 INFO @ Sat, 08 Sep 2018 12:56:11: 16000000 INFO @ Sat, 08 Sep 2018 12:56:11: 15000000 INFO @ Sat, 08 Sep 2018 12:56:14: 16000000 INFO @ Sat, 08 Sep 2018 12:56:17: 17000000 INFO @ Sat, 08 Sep 2018 12:56:19: 16000000 INFO @ Sat, 08 Sep 2018 12:56:21: 17000000 INFO @ Sat, 08 Sep 2018 12:56:25: 18000000 INFO @ Sat, 08 Sep 2018 12:56:27: 17000000 INFO @ Sat, 08 Sep 2018 12:56:28: 18000000 INFO @ Sat, 08 Sep 2018 12:56:32: 19000000 INFO @ Sat, 08 Sep 2018 12:56:35: 18000000 INFO @ Sat, 08 Sep 2018 12:56:35: 19000000 INFO @ Sat, 08 Sep 2018 12:56:39: 20000000 INFO @ Sat, 08 Sep 2018 12:56:43: 20000000 INFO @ Sat, 08 Sep 2018 12:56:43: 19000000 INFO @ Sat, 08 Sep 2018 12:56:46: 21000000 INFO @ Sat, 08 Sep 2018 12:56:50: 21000000 INFO @ Sat, 08 Sep 2018 12:56:51: 20000000 INFO @ Sat, 08 Sep 2018 12:56:54: 22000000 INFO @ Sat, 08 Sep 2018 12:56:57: 22000000 INFO @ Sat, 08 Sep 2018 12:56:59: 21000000 INFO @ Sat, 08 Sep 2018 12:57:01: 23000000 INFO @ Sat, 08 Sep 2018 12:57:04: 23000000 INFO @ Sat, 08 Sep 2018 12:57:07: 22000000 INFO @ Sat, 08 Sep 2018 12:57:08: 24000000 INFO @ Sat, 08 Sep 2018 12:57:11: 24000000 INFO @ Sat, 08 Sep 2018 12:57:15: 23000000 INFO @ Sat, 08 Sep 2018 12:57:15: 25000000 INFO @ Sat, 08 Sep 2018 12:57:18: 25000000 INFO @ Sat, 08 Sep 2018 12:57:23: 26000000 INFO @ Sat, 08 Sep 2018 12:57:23: 24000000 INFO @ Sat, 08 Sep 2018 12:57:25: 26000000 INFO @ Sat, 08 Sep 2018 12:57:30: 27000000 INFO @ Sat, 08 Sep 2018 12:57:30: 25000000 INFO @ Sat, 08 Sep 2018 12:57:32: 27000000 INFO @ Sat, 08 Sep 2018 12:57:37: 28000000 INFO @ Sat, 08 Sep 2018 12:57:37: 26000000 INFO @ Sat, 08 Sep 2018 12:57:40: 28000000 INFO @ Sat, 08 Sep 2018 12:57:44: 29000000 INFO @ Sat, 08 Sep 2018 12:57:45: 27000000 INFO @ Sat, 08 Sep 2018 12:57:47: 29000000 INFO @ Sat, 08 Sep 2018 12:57:52: 30000000 INFO @ Sat, 08 Sep 2018 12:57:52: 28000000 INFO @ Sat, 08 Sep 2018 12:57:55: 30000000 INFO @ Sat, 08 Sep 2018 12:57:59: 31000000 INFO @ Sat, 08 Sep 2018 12:57:59: 29000000 INFO @ Sat, 08 Sep 2018 12:58:02: 31000000 INFO @ Sat, 08 Sep 2018 12:58:07: 32000000 INFO @ Sat, 08 Sep 2018 12:58:07: 30000000 INFO @ Sat, 08 Sep 2018 12:58:10: 32000000 INFO @ Sat, 08 Sep 2018 12:58:14: 33000000 INFO @ Sat, 08 Sep 2018 12:58:14: 31000000 INFO @ Sat, 08 Sep 2018 12:58:17: 33000000 INFO @ Sat, 08 Sep 2018 12:58:21: 34000000 INFO @ Sat, 08 Sep 2018 12:58:22: 32000000 INFO @ Sat, 08 Sep 2018 12:58:24: 34000000 INFO @ Sat, 08 Sep 2018 12:58:29: 35000000 INFO @ Sat, 08 Sep 2018 12:58:29: 33000000 INFO @ Sat, 08 Sep 2018 12:58:32: 35000000 INFO @ Sat, 08 Sep 2018 12:58:36: 34000000 INFO @ Sat, 08 Sep 2018 12:58:36: 36000000 INFO @ Sat, 08 Sep 2018 12:58:39: 36000000 INFO @ Sat, 08 Sep 2018 12:58:43: 35000000 INFO @ Sat, 08 Sep 2018 12:58:44: 37000000 INFO @ Sat, 08 Sep 2018 12:58:47: 37000000 INFO @ Sat, 08 Sep 2018 12:58:50: 36000000 INFO @ Sat, 08 Sep 2018 12:58:51: 38000000 INFO @ Sat, 08 Sep 2018 12:58:54: 38000000 INFO @ Sat, 08 Sep 2018 12:58:58: 37000000 INFO @ Sat, 08 Sep 2018 12:58:58: 39000000 INFO @ Sat, 08 Sep 2018 12:59:02: 39000000 INFO @ Sat, 08 Sep 2018 12:59:05: 38000000 INFO @ Sat, 08 Sep 2018 12:59:06: 40000000 INFO @ Sat, 08 Sep 2018 12:59:09: 40000000 INFO @ Sat, 08 Sep 2018 12:59:12: 39000000 INFO @ Sat, 08 Sep 2018 12:59:13: 41000000 INFO @ Sat, 08 Sep 2018 12:59:16: 41000000 INFO @ Sat, 08 Sep 2018 12:59:19: 40000000 INFO @ Sat, 08 Sep 2018 12:59:20: 42000000 INFO @ Sat, 08 Sep 2018 12:59:24: 42000000 INFO @ Sat, 08 Sep 2018 12:59:26: 41000000 INFO @ Sat, 08 Sep 2018 12:59:28: 43000000 INFO @ Sat, 08 Sep 2018 12:59:31: 43000000 INFO @ Sat, 08 Sep 2018 12:59:33: 42000000 INFO @ Sat, 08 Sep 2018 12:59:35: 44000000 INFO @ Sat, 08 Sep 2018 12:59:36: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:59:36: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:59:36: #1 total tags in treatment: 44112161 INFO @ Sat, 08 Sep 2018 12:59:36: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:59:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:59:37: #1 tags after filtering in treatment: 44112161 INFO @ Sat, 08 Sep 2018 12:59:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:59:37: #1 finished! INFO @ Sat, 08 Sep 2018 12:59:37: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:59:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:59:38: 44000000 INFO @ Sat, 08 Sep 2018 12:59:40: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:59:40: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:59:40: #1 total tags in treatment: 44112161 INFO @ Sat, 08 Sep 2018 12:59:40: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:59:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:59:40: #2 number of paired peaks: 1 WARNING @ Sat, 08 Sep 2018 12:59:40: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:59:40: Process for pairing-model is terminated! cat: SRX2325657.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325657.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325657.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325657.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 12:59:40: #1 tags after filtering in treatment: 44112161 INFO @ Sat, 08 Sep 2018 12:59:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:59:40: #1 finished! INFO @ Sat, 08 Sep 2018 12:59:40: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:59:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:59:40: 43000000 INFO @ Sat, 08 Sep 2018 12:59:44: #2 number of paired peaks: 1 WARNING @ Sat, 08 Sep 2018 12:59:44: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:59:44: Process for pairing-model is terminated! cat: SRX2325657.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325657.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325657.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325657.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 12:59:47: 44000000 INFO @ Sat, 08 Sep 2018 12:59:49: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:59:49: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:59:49: #1 total tags in treatment: 44112161 INFO @ Sat, 08 Sep 2018 12:59:49: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:59:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:59:50: #1 tags after filtering in treatment: 44112161 INFO @ Sat, 08 Sep 2018 12:59:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:59:50: #1 finished! INFO @ Sat, 08 Sep 2018 12:59:50: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:59:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:59:53: #2 number of paired peaks: 1 WARNING @ Sat, 08 Sep 2018 12:59:53: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:59:53: Process for pairing-model is terminated! cat: SRX2325657.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2325657.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325657.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2325657.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。