Job ID = 6527704 SRX = SRX2325647 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:08:25 prefetch.2.10.7: 1) Downloading 'SRR4896335'... 2020-06-29T13:08:25 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:13:50 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:13:50 prefetch.2.10.7: 1) 'SRR4896335' was downloaded successfully 2020-06-29T13:13:50 prefetch.2.10.7: 'SRR4896335' has 0 unresolved dependencies Read 47011860 spots for SRR4896335/SRR4896335.sra Written 47011860 spots for SRR4896335/SRR4896335.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:46 47011860 reads; of these: 47011860 (100.00%) were unpaired; of these: 5803105 (12.34%) aligned 0 times 30694075 (65.29%) aligned exactly 1 time 10514680 (22.37%) aligned >1 times 87.66% overall alignment rate Time searching: 00:19:46 Overall time: 00:19:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7590879 / 41208755 = 0.1842 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:00:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:00:26: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:00:26: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:00:31: 1000000 INFO @ Mon, 29 Jun 2020 23:00:37: 2000000 INFO @ Mon, 29 Jun 2020 23:00:42: 3000000 INFO @ Mon, 29 Jun 2020 23:00:47: 4000000 INFO @ Mon, 29 Jun 2020 23:00:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:00:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:00:56: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:00:56: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:00:58: 6000000 INFO @ Mon, 29 Jun 2020 23:01:01: 1000000 INFO @ Mon, 29 Jun 2020 23:01:04: 7000000 INFO @ Mon, 29 Jun 2020 23:01:07: 2000000 INFO @ Mon, 29 Jun 2020 23:01:09: 8000000 INFO @ Mon, 29 Jun 2020 23:01:13: 3000000 INFO @ Mon, 29 Jun 2020 23:01:15: 9000000 INFO @ Mon, 29 Jun 2020 23:01:18: 4000000 INFO @ Mon, 29 Jun 2020 23:01:21: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:01:24: 5000000 INFO @ Mon, 29 Jun 2020 23:01:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:01:26: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:01:26: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:01:26: 11000000 INFO @ Mon, 29 Jun 2020 23:01:30: 6000000 INFO @ Mon, 29 Jun 2020 23:01:32: 12000000 INFO @ Mon, 29 Jun 2020 23:01:33: 1000000 INFO @ Mon, 29 Jun 2020 23:01:36: 7000000 INFO @ Mon, 29 Jun 2020 23:01:38: 13000000 INFO @ Mon, 29 Jun 2020 23:01:40: 2000000 INFO @ Mon, 29 Jun 2020 23:01:42: 8000000 INFO @ Mon, 29 Jun 2020 23:01:44: 14000000 INFO @ Mon, 29 Jun 2020 23:01:47: 3000000 INFO @ Mon, 29 Jun 2020 23:01:48: 9000000 INFO @ Mon, 29 Jun 2020 23:01:50: 15000000 INFO @ Mon, 29 Jun 2020 23:01:54: 10000000 INFO @ Mon, 29 Jun 2020 23:01:54: 4000000 INFO @ Mon, 29 Jun 2020 23:01:56: 16000000 INFO @ Mon, 29 Jun 2020 23:01:59: 11000000 INFO @ Mon, 29 Jun 2020 23:02:01: 5000000 INFO @ Mon, 29 Jun 2020 23:02:02: 17000000 INFO @ Mon, 29 Jun 2020 23:02:05: 12000000 INFO @ Mon, 29 Jun 2020 23:02:08: 18000000 INFO @ Mon, 29 Jun 2020 23:02:08: 6000000 INFO @ Mon, 29 Jun 2020 23:02:11: 13000000 INFO @ Mon, 29 Jun 2020 23:02:13: 19000000 INFO @ Mon, 29 Jun 2020 23:02:14: 7000000 INFO @ Mon, 29 Jun 2020 23:02:17: 14000000 INFO @ Mon, 29 Jun 2020 23:02:19: 20000000 INFO @ Mon, 29 Jun 2020 23:02:21: 8000000 INFO @ Mon, 29 Jun 2020 23:02:23: 15000000 INFO @ Mon, 29 Jun 2020 23:02:25: 21000000 INFO @ Mon, 29 Jun 2020 23:02:27: 9000000 INFO @ Mon, 29 Jun 2020 23:02:29: 16000000 INFO @ Mon, 29 Jun 2020 23:02:31: 22000000 INFO @ Mon, 29 Jun 2020 23:02:34: 10000000 INFO @ Mon, 29 Jun 2020 23:02:35: 17000000 INFO @ Mon, 29 Jun 2020 23:02:37: 23000000 INFO @ Mon, 29 Jun 2020 23:02:41: 11000000 INFO @ Mon, 29 Jun 2020 23:02:41: 18000000 INFO @ Mon, 29 Jun 2020 23:02:43: 24000000 INFO @ Mon, 29 Jun 2020 23:02:47: 19000000 INFO @ Mon, 29 Jun 2020 23:02:47: 12000000 INFO @ Mon, 29 Jun 2020 23:02:48: 25000000 INFO @ Mon, 29 Jun 2020 23:02:52: 20000000 INFO @ Mon, 29 Jun 2020 23:02:54: 13000000 INFO @ Mon, 29 Jun 2020 23:02:54: 26000000 INFO @ Mon, 29 Jun 2020 23:02:58: 21000000 INFO @ Mon, 29 Jun 2020 23:03:00: 27000000 INFO @ Mon, 29 Jun 2020 23:03:01: 14000000 INFO @ Mon, 29 Jun 2020 23:03:04: 22000000 INFO @ Mon, 29 Jun 2020 23:03:06: 28000000 INFO @ Mon, 29 Jun 2020 23:03:07: 15000000 INFO @ Mon, 29 Jun 2020 23:03:10: 23000000 INFO @ Mon, 29 Jun 2020 23:03:12: 29000000 INFO @ Mon, 29 Jun 2020 23:03:14: 16000000 INFO @ Mon, 29 Jun 2020 23:03:16: 24000000 INFO @ Mon, 29 Jun 2020 23:03:18: 30000000 INFO @ Mon, 29 Jun 2020 23:03:20: 17000000 INFO @ Mon, 29 Jun 2020 23:03:22: 25000000 INFO @ Mon, 29 Jun 2020 23:03:24: 31000000 INFO @ Mon, 29 Jun 2020 23:03:27: 18000000 INFO @ Mon, 29 Jun 2020 23:03:27: 26000000 INFO @ Mon, 29 Jun 2020 23:03:30: 32000000 INFO @ Mon, 29 Jun 2020 23:03:33: 19000000 INFO @ Mon, 29 Jun 2020 23:03:33: 27000000 INFO @ Mon, 29 Jun 2020 23:03:36: 33000000 INFO @ Mon, 29 Jun 2020 23:03:39: 28000000 INFO @ Mon, 29 Jun 2020 23:03:40: 20000000 INFO @ Mon, 29 Jun 2020 23:03:40: #1 tag size is determined as 76 bps INFO @ Mon, 29 Jun 2020 23:03:40: #1 tag size = 76 INFO @ Mon, 29 Jun 2020 23:03:40: #1 total tags in treatment: 33617876 INFO @ Mon, 29 Jun 2020 23:03:40: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:03:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:03:40: #1 tags after filtering in treatment: 33617876 INFO @ Mon, 29 Jun 2020 23:03:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:03:40: #1 finished! INFO @ Mon, 29 Jun 2020 23:03:40: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:03:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:03:42: #2 number of paired peaks: 47 WARNING @ Mon, 29 Jun 2020 23:03:42: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:03:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:03:45: 29000000 INFO @ Mon, 29 Jun 2020 23:03:46: 21000000 INFO @ Mon, 29 Jun 2020 23:03:51: 30000000 INFO @ Mon, 29 Jun 2020 23:03:53: 22000000 INFO @ Mon, 29 Jun 2020 23:03:57: 31000000 INFO @ Mon, 29 Jun 2020 23:03:59: 23000000 INFO @ Mon, 29 Jun 2020 23:04:03: 32000000 INFO @ Mon, 29 Jun 2020 23:04:05: 24000000 INFO @ Mon, 29 Jun 2020 23:04:08: 33000000 INFO @ Mon, 29 Jun 2020 23:04:12: 25000000 INFO @ Mon, 29 Jun 2020 23:04:12: #1 tag size is determined as 76 bps INFO @ Mon, 29 Jun 2020 23:04:12: #1 tag size = 76 INFO @ Mon, 29 Jun 2020 23:04:12: #1 total tags in treatment: 33617876 INFO @ Mon, 29 Jun 2020 23:04:12: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:04:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:04:13: #1 tags after filtering in treatment: 33617876 INFO @ Mon, 29 Jun 2020 23:04:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:04:13: #1 finished! INFO @ Mon, 29 Jun 2020 23:04:13: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:04:13: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:04:15: #2 number of paired peaks: 47 WARNING @ Mon, 29 Jun 2020 23:04:15: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:04:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:04:18: 26000000 INFO @ Mon, 29 Jun 2020 23:04:25: 27000000 INFO @ Mon, 29 Jun 2020 23:04:31: 28000000 INFO @ Mon, 29 Jun 2020 23:04:38: 29000000 INFO @ Mon, 29 Jun 2020 23:04:44: 30000000 INFO @ Mon, 29 Jun 2020 23:04:50: 31000000 INFO @ Mon, 29 Jun 2020 23:04:57: 32000000 INFO @ Mon, 29 Jun 2020 23:05:03: 33000000 INFO @ Mon, 29 Jun 2020 23:05:07: #1 tag size is determined as 76 bps INFO @ Mon, 29 Jun 2020 23:05:07: #1 tag size = 76 INFO @ Mon, 29 Jun 2020 23:05:07: #1 total tags in treatment: 33617876 INFO @ Mon, 29 Jun 2020 23:05:07: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:05:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:05:07: #1 tags after filtering in treatment: 33617876 INFO @ Mon, 29 Jun 2020 23:05:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:05:07: #1 finished! INFO @ Mon, 29 Jun 2020 23:05:07: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:05:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:05:09: #2 number of paired peaks: 47 WARNING @ Mon, 29 Jun 2020 23:05:09: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:05:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2325647/SRX2325647.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。