Job ID = 9158686 sra ファイルのダウンロード中... Completed: 992305K bytes transferred in 12 seconds (661569K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 44189125 spots for /home/okishinya/chipatlas/results/dm3/SRX2172426/SRR4252437.sra Written 44189125 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:08 44189125 reads; of these: 44189125 (100.00%) were unpaired; of these: 1730258 (3.92%) aligned 0 times 32019392 (72.46%) aligned exactly 1 time 10439475 (23.62%) aligned >1 times 96.08% overall alignment rate Time searching: 00:16:08 Overall time: 00:16:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5778416 / 42458867 = 0.1361 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 19:35:43: # Command line: callpeak -t SRX2172426.bam -f BAM -g dm -n SRX2172426.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2172426.05 # format = BAM # ChIP-seq file = ['SRX2172426.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:35:43: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:35:43: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:35:43: # Command line: callpeak -t SRX2172426.bam -f BAM -g dm -n SRX2172426.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2172426.20 # format = BAM # ChIP-seq file = ['SRX2172426.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:35:43: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:35:43: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:35:43: # Command line: callpeak -t SRX2172426.bam -f BAM -g dm -n SRX2172426.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2172426.10 # format = BAM # ChIP-seq file = ['SRX2172426.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:35:43: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:35:43: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:35:51: 1000000 INFO @ Tue, 27 Jun 2017 19:35:51: 1000000 INFO @ Tue, 27 Jun 2017 19:35:51: 1000000 INFO @ Tue, 27 Jun 2017 19:35:58: 2000000 INFO @ Tue, 27 Jun 2017 19:35:58: 2000000 INFO @ Tue, 27 Jun 2017 19:35:58: 2000000 INFO @ Tue, 27 Jun 2017 19:36:05: 3000000 INFO @ Tue, 27 Jun 2017 19:36:05: 3000000 INFO @ Tue, 27 Jun 2017 19:36:06: 3000000 INFO @ Tue, 27 Jun 2017 19:36:12: 4000000 INFO @ Tue, 27 Jun 2017 19:36:12: 4000000 INFO @ Tue, 27 Jun 2017 19:36:13: 4000000 INFO @ Tue, 27 Jun 2017 19:36:20: 5000000 INFO @ Tue, 27 Jun 2017 19:36:20: 5000000 INFO @ Tue, 27 Jun 2017 19:36:21: 5000000 INFO @ Tue, 27 Jun 2017 19:36:27: 6000000 INFO @ Tue, 27 Jun 2017 19:36:27: 6000000 INFO @ Tue, 27 Jun 2017 19:36:28: 6000000 INFO @ Tue, 27 Jun 2017 19:36:34: 7000000 INFO @ Tue, 27 Jun 2017 19:36:34: 7000000 INFO @ Tue, 27 Jun 2017 19:36:35: 7000000 INFO @ Tue, 27 Jun 2017 19:36:42: 8000000 INFO @ Tue, 27 Jun 2017 19:36:42: 8000000 INFO @ Tue, 27 Jun 2017 19:36:42: 8000000 INFO @ Tue, 27 Jun 2017 19:36:49: 9000000 INFO @ Tue, 27 Jun 2017 19:36:49: 9000000 INFO @ Tue, 27 Jun 2017 19:36:50: 9000000 INFO @ Tue, 27 Jun 2017 19:36:56: 10000000 INFO @ Tue, 27 Jun 2017 19:36:56: 10000000 INFO @ Tue, 27 Jun 2017 19:36:56: 10000000 INFO @ Tue, 27 Jun 2017 19:37:02: 11000000 INFO @ Tue, 27 Jun 2017 19:37:03: 11000000 INFO @ Tue, 27 Jun 2017 19:37:03: 11000000 INFO @ Tue, 27 Jun 2017 19:37:08: 12000000 INFO @ Tue, 27 Jun 2017 19:37:09: 12000000 INFO @ Tue, 27 Jun 2017 19:37:09: 12000000 INFO @ Tue, 27 Jun 2017 19:37:14: 13000000 INFO @ Tue, 27 Jun 2017 19:37:15: 13000000 INFO @ Tue, 27 Jun 2017 19:37:15: 13000000 INFO @ Tue, 27 Jun 2017 19:37:20: 14000000 INFO @ Tue, 27 Jun 2017 19:37:22: 14000000 INFO @ Tue, 27 Jun 2017 19:37:22: 14000000 INFO @ Tue, 27 Jun 2017 19:37:26: 15000000 INFO @ Tue, 27 Jun 2017 19:37:28: 15000000 INFO @ Tue, 27 Jun 2017 19:37:28: 15000000 INFO @ Tue, 27 Jun 2017 19:37:31: 16000000 INFO @ Tue, 27 Jun 2017 19:37:34: 16000000 INFO @ Tue, 27 Jun 2017 19:37:34: 16000000 INFO @ Tue, 27 Jun 2017 19:37:37: 17000000 INFO @ Tue, 27 Jun 2017 19:37:41: 17000000 INFO @ Tue, 27 Jun 2017 19:37:41: 17000000 INFO @ Tue, 27 Jun 2017 19:37:43: 18000000 INFO @ Tue, 27 Jun 2017 19:37:47: 18000000 INFO @ Tue, 27 Jun 2017 19:37:47: 18000000 INFO @ Tue, 27 Jun 2017 19:37:49: 19000000 INFO @ Tue, 27 Jun 2017 19:37:54: 19000000 INFO @ Tue, 27 Jun 2017 19:37:54: 19000000 INFO @ Tue, 27 Jun 2017 19:37:55: 20000000 INFO @ Tue, 27 Jun 2017 19:38:00: 20000000 INFO @ Tue, 27 Jun 2017 19:38:00: 20000000 INFO @ Tue, 27 Jun 2017 19:38:01: 21000000 INFO @ Tue, 27 Jun 2017 19:38:06: 21000000 INFO @ Tue, 27 Jun 2017 19:38:06: 21000000 INFO @ Tue, 27 Jun 2017 19:38:06: 22000000 INFO @ Tue, 27 Jun 2017 19:38:12: 23000000 INFO @ Tue, 27 Jun 2017 19:38:12: 22000000 INFO @ Tue, 27 Jun 2017 19:38:12: 22000000 INFO @ Tue, 27 Jun 2017 19:38:18: 24000000 INFO @ Tue, 27 Jun 2017 19:38:19: 23000000 INFO @ Tue, 27 Jun 2017 19:38:19: 23000000 INFO @ Tue, 27 Jun 2017 19:38:24: 25000000 INFO @ Tue, 27 Jun 2017 19:38:25: 24000000 INFO @ Tue, 27 Jun 2017 19:38:25: 24000000 INFO @ Tue, 27 Jun 2017 19:38:30: 26000000 INFO @ Tue, 27 Jun 2017 19:38:32: 25000000 INFO @ Tue, 27 Jun 2017 19:38:32: 25000000 INFO @ Tue, 27 Jun 2017 19:38:36: 27000000 INFO @ Tue, 27 Jun 2017 19:38:38: 26000000 INFO @ Tue, 27 Jun 2017 19:38:38: 26000000 INFO @ Tue, 27 Jun 2017 19:38:41: 28000000 INFO @ Tue, 27 Jun 2017 19:38:44: 27000000 INFO @ Tue, 27 Jun 2017 19:38:44: 27000000 INFO @ Tue, 27 Jun 2017 19:38:47: 29000000 INFO @ Tue, 27 Jun 2017 19:38:51: 28000000 INFO @ Tue, 27 Jun 2017 19:38:51: 28000000 INFO @ Tue, 27 Jun 2017 19:38:53: 30000000 INFO @ Tue, 27 Jun 2017 19:38:57: 29000000 INFO @ Tue, 27 Jun 2017 19:38:57: 29000000 INFO @ Tue, 27 Jun 2017 19:38:59: 31000000 INFO @ Tue, 27 Jun 2017 19:39:03: 30000000 INFO @ Tue, 27 Jun 2017 19:39:03: 30000000 INFO @ Tue, 27 Jun 2017 19:39:05: 32000000 INFO @ Tue, 27 Jun 2017 19:39:09: 31000000 INFO @ Tue, 27 Jun 2017 19:39:09: 31000000 INFO @ Tue, 27 Jun 2017 19:39:11: 33000000 INFO @ Tue, 27 Jun 2017 19:39:16: 32000000 INFO @ Tue, 27 Jun 2017 19:39:16: 32000000 INFO @ Tue, 27 Jun 2017 19:39:17: 34000000 INFO @ Tue, 27 Jun 2017 19:39:22: 33000000 INFO @ Tue, 27 Jun 2017 19:39:22: 33000000 INFO @ Tue, 27 Jun 2017 19:39:22: 35000000 INFO @ Tue, 27 Jun 2017 19:39:28: 36000000 INFO @ Tue, 27 Jun 2017 19:39:29: 34000000 INFO @ Tue, 27 Jun 2017 19:39:29: 34000000 INFO @ Tue, 27 Jun 2017 19:39:33: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 19:39:33: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 19:39:33: #1 total tags in treatment: 36680451 INFO @ Tue, 27 Jun 2017 19:39:33: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:39:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:39:33: #1 tags after filtering in treatment: 36680451 INFO @ Tue, 27 Jun 2017 19:39:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:39:33: #1 finished! INFO @ Tue, 27 Jun 2017 19:39:33: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:39:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:39:36: #2 number of paired peaks: 90 WARNING @ Tue, 27 Jun 2017 19:39:36: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:39:36: Process for pairing-model is terminated! cat: SRX2172426.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2172426.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2172426.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2172426.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:39:36: 35000000 INFO @ Tue, 27 Jun 2017 19:39:36: 35000000 INFO @ Tue, 27 Jun 2017 19:39:42: 36000000 INFO @ Tue, 27 Jun 2017 19:39:43: 36000000 INFO @ Tue, 27 Jun 2017 19:39:47: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 19:39:47: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 19:39:47: #1 total tags in treatment: 36680451 INFO @ Tue, 27 Jun 2017 19:39:47: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:39:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:39:47: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 19:39:47: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 19:39:47: #1 total tags in treatment: 36680451 INFO @ Tue, 27 Jun 2017 19:39:47: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:39:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:39:47: #1 tags after filtering in treatment: 36680451 INFO @ Tue, 27 Jun 2017 19:39:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:39:47: #1 finished! INFO @ Tue, 27 Jun 2017 19:39:47: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:39:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:39:48: #1 tags after filtering in treatment: 36680451 INFO @ Tue, 27 Jun 2017 19:39:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:39:48: #1 finished! INFO @ Tue, 27 Jun 2017 19:39:48: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:39:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:39:50: #2 number of paired peaks: 90 WARNING @ Tue, 27 Jun 2017 19:39:50: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:39:50: Process for pairing-model is terminated! cat: SRX2172426.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2172426.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2172426.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2172426.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:39:50: #2 number of paired peaks: 90 WARNING @ Tue, 27 Jun 2017 19:39:50: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:39:50: Process for pairing-model is terminated! cat: SRX2172426.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2172426.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2172426.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2172426.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。