Job ID = 11293651 sra ファイルのダウンロード中... Completed: 1910072K bytes transferred in 62 seconds (250397K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 75985128 spots for /home/okishinya/chipatlas/results/dm3/SRX2068665/SRR4101021.sra Written 75985128 spots for /home/okishinya/chipatlas/results/dm3/SRX2068665/SRR4101021.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:06 75985128 reads; of these: 75985128 (100.00%) were unpaired; of these: 11618301 (15.29%) aligned 0 times 46539461 (61.25%) aligned exactly 1 time 17827366 (23.46%) aligned >1 times 84.71% overall alignment rate Time searching: 00:23:06 Overall time: 00:23:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 32309255 / 64366827 = 0.5020 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 04 Nov 2018 18:35:45: # Command line: callpeak -t SRX2068665.bam -f BAM -g dm -n SRX2068665.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2068665.05 # format = BAM # ChIP-seq file = ['SRX2068665.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 04 Nov 2018 18:35:45: #1 read tag files... INFO @ Sun, 04 Nov 2018 18:35:45: #1 read treatment tags... INFO @ Sun, 04 Nov 2018 18:35:45: # Command line: callpeak -t SRX2068665.bam -f BAM -g dm -n SRX2068665.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2068665.10 # format = BAM # ChIP-seq file = ['SRX2068665.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 04 Nov 2018 18:35:45: #1 read tag files... INFO @ Sun, 04 Nov 2018 18:35:45: #1 read treatment tags... INFO @ Sun, 04 Nov 2018 18:35:45: # Command line: callpeak -t SRX2068665.bam -f BAM -g dm -n SRX2068665.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2068665.20 # format = BAM # ChIP-seq file = ['SRX2068665.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 04 Nov 2018 18:35:45: #1 read tag files... INFO @ Sun, 04 Nov 2018 18:35:45: #1 read treatment tags... INFO @ Sun, 04 Nov 2018 18:35:51: 1000000 INFO @ Sun, 04 Nov 2018 18:35:51: 1000000 INFO @ Sun, 04 Nov 2018 18:35:51: 1000000 INFO @ Sun, 04 Nov 2018 18:35:57: 2000000 INFO @ Sun, 04 Nov 2018 18:35:57: 2000000 INFO @ Sun, 04 Nov 2018 18:35:58: 2000000 INFO @ Sun, 04 Nov 2018 18:36:03: 3000000 INFO @ Sun, 04 Nov 2018 18:36:03: 3000000 INFO @ Sun, 04 Nov 2018 18:36:04: 3000000 INFO @ Sun, 04 Nov 2018 18:36:09: 4000000 INFO @ Sun, 04 Nov 2018 18:36:09: 4000000 INFO @ Sun, 04 Nov 2018 18:36:10: 4000000 INFO @ Sun, 04 Nov 2018 18:36:15: 5000000 INFO @ Sun, 04 Nov 2018 18:36:15: 5000000 INFO @ Sun, 04 Nov 2018 18:36:17: 5000000 INFO @ Sun, 04 Nov 2018 18:36:21: 6000000 INFO @ Sun, 04 Nov 2018 18:36:22: 6000000 INFO @ Sun, 04 Nov 2018 18:36:23: 6000000 INFO @ Sun, 04 Nov 2018 18:36:27: 7000000 INFO @ Sun, 04 Nov 2018 18:36:28: 7000000 INFO @ Sun, 04 Nov 2018 18:36:30: 7000000 INFO @ Sun, 04 Nov 2018 18:36:33: 8000000 INFO @ Sun, 04 Nov 2018 18:36:34: 8000000 INFO @ Sun, 04 Nov 2018 18:36:36: 8000000 INFO @ Sun, 04 Nov 2018 18:36:39: 9000000 INFO @ Sun, 04 Nov 2018 18:36:40: 9000000 INFO @ Sun, 04 Nov 2018 18:36:42: 9000000 INFO @ Sun, 04 Nov 2018 18:36:45: 10000000 INFO @ Sun, 04 Nov 2018 18:36:46: 10000000 INFO @ Sun, 04 Nov 2018 18:36:48: 10000000 INFO @ Sun, 04 Nov 2018 18:36:51: 11000000 INFO @ Sun, 04 Nov 2018 18:36:52: 11000000 INFO @ Sun, 04 Nov 2018 18:36:54: 11000000 INFO @ Sun, 04 Nov 2018 18:36:57: 12000000 INFO @ Sun, 04 Nov 2018 18:36:58: 12000000 INFO @ Sun, 04 Nov 2018 18:37:00: 12000000 INFO @ Sun, 04 Nov 2018 18:37:03: 13000000 INFO @ Sun, 04 Nov 2018 18:37:04: 13000000 INFO @ Sun, 04 Nov 2018 18:37:06: 13000000 INFO @ Sun, 04 Nov 2018 18:37:09: 14000000 INFO @ Sun, 04 Nov 2018 18:37:11: 14000000 INFO @ Sun, 04 Nov 2018 18:37:12: 14000000 INFO @ Sun, 04 Nov 2018 18:37:15: 15000000 INFO @ Sun, 04 Nov 2018 18:37:17: 15000000 INFO @ Sun, 04 Nov 2018 18:37:18: 15000000 INFO @ Sun, 04 Nov 2018 18:37:21: 16000000 INFO @ Sun, 04 Nov 2018 18:37:23: 16000000 INFO @ Sun, 04 Nov 2018 18:37:24: 16000000 INFO @ Sun, 04 Nov 2018 18:37:27: 17000000 INFO @ Sun, 04 Nov 2018 18:37:29: 17000000 INFO @ Sun, 04 Nov 2018 18:37:30: 17000000 INFO @ Sun, 04 Nov 2018 18:37:33: 18000000 INFO @ Sun, 04 Nov 2018 18:37:35: 18000000 INFO @ Sun, 04 Nov 2018 18:37:37: 18000000 INFO @ Sun, 04 Nov 2018 18:37:40: 19000000 INFO @ Sun, 04 Nov 2018 18:37:41: 19000000 INFO @ Sun, 04 Nov 2018 18:37:43: 19000000 INFO @ Sun, 04 Nov 2018 18:37:46: 20000000 INFO @ Sun, 04 Nov 2018 18:37:48: 20000000 INFO @ Sun, 04 Nov 2018 18:37:49: 20000000 INFO @ Sun, 04 Nov 2018 18:37:52: 21000000 INFO @ Sun, 04 Nov 2018 18:37:54: 21000000 INFO @ Sun, 04 Nov 2018 18:37:55: 21000000 INFO @ Sun, 04 Nov 2018 18:37:58: 22000000 INFO @ Sun, 04 Nov 2018 18:38:00: 22000000 INFO @ Sun, 04 Nov 2018 18:38:02: 22000000 INFO @ Sun, 04 Nov 2018 18:38:05: 23000000 INFO @ Sun, 04 Nov 2018 18:38:06: 23000000 INFO @ Sun, 04 Nov 2018 18:38:08: 23000000 INFO @ Sun, 04 Nov 2018 18:38:11: 24000000 INFO @ Sun, 04 Nov 2018 18:38:12: 24000000 INFO @ Sun, 04 Nov 2018 18:38:14: 24000000 INFO @ Sun, 04 Nov 2018 18:38:17: 25000000 INFO @ Sun, 04 Nov 2018 18:38:19: 25000000 INFO @ Sun, 04 Nov 2018 18:38:20: 25000000 INFO @ Sun, 04 Nov 2018 18:38:23: 26000000 INFO @ Sun, 04 Nov 2018 18:38:25: 26000000 INFO @ Sun, 04 Nov 2018 18:38:26: 26000000 INFO @ Sun, 04 Nov 2018 18:38:29: 27000000 INFO @ Sun, 04 Nov 2018 18:38:31: 27000000 INFO @ Sun, 04 Nov 2018 18:38:32: 27000000 INFO @ Sun, 04 Nov 2018 18:38:35: 28000000 INFO @ Sun, 04 Nov 2018 18:38:37: 28000000 INFO @ Sun, 04 Nov 2018 18:38:39: 28000000 INFO @ Sun, 04 Nov 2018 18:38:41: 29000000 INFO @ Sun, 04 Nov 2018 18:38:44: 29000000 INFO @ Sun, 04 Nov 2018 18:38:45: 29000000 INFO @ Sun, 04 Nov 2018 18:38:47: 30000000 INFO @ Sun, 04 Nov 2018 18:38:50: 30000000 INFO @ Sun, 04 Nov 2018 18:38:51: 30000000 INFO @ Sun, 04 Nov 2018 18:38:53: 31000000 INFO @ Sun, 04 Nov 2018 18:38:56: 31000000 INFO @ Sun, 04 Nov 2018 18:38:57: 31000000 INFO @ Sun, 04 Nov 2018 18:38:59: 32000000 INFO @ Sun, 04 Nov 2018 18:39:00: #1 tag size is determined as 50 bps INFO @ Sun, 04 Nov 2018 18:39:00: #1 tag size = 50 INFO @ Sun, 04 Nov 2018 18:39:00: #1 total tags in treatment: 32057572 INFO @ Sun, 04 Nov 2018 18:39:00: #1 user defined the maximum tags... INFO @ Sun, 04 Nov 2018 18:39:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 04 Nov 2018 18:39:00: #1 tags after filtering in treatment: 32057572 INFO @ Sun, 04 Nov 2018 18:39:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 04 Nov 2018 18:39:00: #1 finished! INFO @ Sun, 04 Nov 2018 18:39:00: #2 Build Peak Model... INFO @ Sun, 04 Nov 2018 18:39:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 04 Nov 2018 18:39:02: 32000000 INFO @ Sun, 04 Nov 2018 18:39:02: #2 number of paired peaks: 43 WARNING @ Sun, 04 Nov 2018 18:39:02: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 04 Nov 2018 18:39:02: Process for pairing-model is terminated! cat: SRX2068665.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2068665.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2068665.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2068665.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 04 Nov 2018 18:39:03: #1 tag size is determined as 50 bps INFO @ Sun, 04 Nov 2018 18:39:03: #1 tag size = 50 INFO @ Sun, 04 Nov 2018 18:39:03: #1 total tags in treatment: 32057572 INFO @ Sun, 04 Nov 2018 18:39:03: #1 user defined the maximum tags... INFO @ Sun, 04 Nov 2018 18:39:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 04 Nov 2018 18:39:03: 32000000 INFO @ Sun, 04 Nov 2018 18:39:03: #1 tags after filtering in treatment: 32057572 INFO @ Sun, 04 Nov 2018 18:39:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 04 Nov 2018 18:39:03: #1 finished! INFO @ Sun, 04 Nov 2018 18:39:03: #2 Build Peak Model... INFO @ Sun, 04 Nov 2018 18:39:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 04 Nov 2018 18:39:03: #1 tag size is determined as 50 bps INFO @ Sun, 04 Nov 2018 18:39:03: #1 tag size = 50 INFO @ Sun, 04 Nov 2018 18:39:03: #1 total tags in treatment: 32057572 INFO @ Sun, 04 Nov 2018 18:39:03: #1 user defined the maximum tags... INFO @ Sun, 04 Nov 2018 18:39:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 04 Nov 2018 18:39:04: #1 tags after filtering in treatment: 32057572 INFO @ Sun, 04 Nov 2018 18:39:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 04 Nov 2018 18:39:04: #1 finished! INFO @ Sun, 04 Nov 2018 18:39:04: #2 Build Peak Model... INFO @ Sun, 04 Nov 2018 18:39:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 04 Nov 2018 18:39:05: #2 number of paired peaks: 43 WARNING @ Sun, 04 Nov 2018 18:39:05: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 04 Nov 2018 18:39:05: Process for pairing-model is terminated! cat: SRX2068665.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2068665.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2068665.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2068665.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 04 Nov 2018 18:39:06: #2 number of paired peaks: 43 WARNING @ Sun, 04 Nov 2018 18:39:06: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 04 Nov 2018 18:39:06: Process for pairing-model is terminated! cat: SRX2068665.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2068665.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2068665.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2068665.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。