Job ID = 6527675 SRX = SRX202832 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:40:26 prefetch.2.10.7: 1) Downloading 'SRR611193'... 2020-06-29T13:40:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:41:54 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:41:55 prefetch.2.10.7: 'SRR611193' is valid 2020-06-29T13:41:55 prefetch.2.10.7: 1) 'SRR611193' was downloaded successfully Read 9740787 spots for SRR611193/SRR611193.sra Written 9740787 spots for SRR611193/SRR611193.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:12 9740787 reads; of these: 9740787 (100.00%) were unpaired; of these: 232461 (2.39%) aligned 0 times 7753840 (79.60%) aligned exactly 1 time 1754486 (18.01%) aligned >1 times 97.61% overall alignment rate Time searching: 00:04:12 Overall time: 00:04:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 561330 / 9508326 = 0.0590 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:55:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:55:45: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:55:45: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:55:51: 1000000 INFO @ Mon, 29 Jun 2020 22:55:58: 2000000 INFO @ Mon, 29 Jun 2020 22:56:04: 3000000 INFO @ Mon, 29 Jun 2020 22:56:11: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:56:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:56:15: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:56:15: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:56:17: 5000000 INFO @ Mon, 29 Jun 2020 22:56:21: 1000000 INFO @ Mon, 29 Jun 2020 22:56:24: 6000000 INFO @ Mon, 29 Jun 2020 22:56:27: 2000000 INFO @ Mon, 29 Jun 2020 22:56:31: 7000000 INFO @ Mon, 29 Jun 2020 22:56:33: 3000000 INFO @ Mon, 29 Jun 2020 22:56:38: 8000000 INFO @ Mon, 29 Jun 2020 22:56:40: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:56:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:56:45: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:56:45: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:56:45: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 22:56:45: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 22:56:45: #1 total tags in treatment: 8946996 INFO @ Mon, 29 Jun 2020 22:56:45: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:56:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:56:45: #1 tags after filtering in treatment: 8946996 INFO @ Mon, 29 Jun 2020 22:56:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:56:45: #1 finished! INFO @ Mon, 29 Jun 2020 22:56:45: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:56:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:56:46: #2 number of paired peaks: 38 WARNING @ Mon, 29 Jun 2020 22:56:46: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:56:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:56:46: 5000000 INFO @ Mon, 29 Jun 2020 22:56:51: 1000000 INFO @ Mon, 29 Jun 2020 22:56:52: 6000000 INFO @ Mon, 29 Jun 2020 22:56:58: 7000000 INFO @ Mon, 29 Jun 2020 22:56:58: 2000000 INFO @ Mon, 29 Jun 2020 22:57:04: 8000000 INFO @ Mon, 29 Jun 2020 22:57:05: 3000000 INFO @ Mon, 29 Jun 2020 22:57:10: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 22:57:10: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 22:57:10: #1 total tags in treatment: 8946996 INFO @ Mon, 29 Jun 2020 22:57:10: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:57:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:57:11: #1 tags after filtering in treatment: 8946996 INFO @ Mon, 29 Jun 2020 22:57:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:57:11: #1 finished! INFO @ Mon, 29 Jun 2020 22:57:11: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:57:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:57:11: #2 number of paired peaks: 38 WARNING @ Mon, 29 Jun 2020 22:57:11: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:57:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:57:12: 4000000 INFO @ Mon, 29 Jun 2020 22:57:18: 5000000 INFO @ Mon, 29 Jun 2020 22:57:25: 6000000 INFO @ Mon, 29 Jun 2020 22:57:32: 7000000 INFO @ Mon, 29 Jun 2020 22:57:38: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:57:45: #1 tag size is determined as 75 bps INFO @ Mon, 29 Jun 2020 22:57:45: #1 tag size = 75 INFO @ Mon, 29 Jun 2020 22:57:45: #1 total tags in treatment: 8946996 INFO @ Mon, 29 Jun 2020 22:57:45: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:57:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:57:45: #1 tags after filtering in treatment: 8946996 INFO @ Mon, 29 Jun 2020 22:57:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:57:45: #1 finished! INFO @ Mon, 29 Jun 2020 22:57:45: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:57:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:57:46: #2 number of paired peaks: 38 WARNING @ Mon, 29 Jun 2020 22:57:46: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:57:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX202832/SRX202832.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。