Job ID = 1294203 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,914,098 reads read : 8,914,098 reads written : 8,914,098 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:43 8914098 reads; of these: 8914098 (100.00%) were unpaired; of these: 197206 (2.21%) aligned 0 times 2381471 (26.72%) aligned exactly 1 time 6335421 (71.07%) aligned >1 times 97.79% overall alignment rate Time searching: 00:09:43 Overall time: 00:09:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 2221360 / 8716892 = 0.2548 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 05:42:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:42:19: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:42:19: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:42:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:42:19: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:42:19: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:42:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:42:19: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:42:19: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:42:28: 1000000 INFO @ Mon, 03 Jun 2019 05:42:31: 1000000 INFO @ Mon, 03 Jun 2019 05:42:31: 1000000 INFO @ Mon, 03 Jun 2019 05:42:36: 2000000 INFO @ Mon, 03 Jun 2019 05:42:41: 2000000 INFO @ Mon, 03 Jun 2019 05:42:42: 2000000 INFO @ Mon, 03 Jun 2019 05:42:45: 3000000 INFO @ Mon, 03 Jun 2019 05:42:52: 3000000 INFO @ Mon, 03 Jun 2019 05:42:53: 4000000 INFO @ Mon, 03 Jun 2019 05:42:54: 3000000 INFO @ Mon, 03 Jun 2019 05:43:01: 5000000 INFO @ Mon, 03 Jun 2019 05:43:02: 4000000 INFO @ Mon, 03 Jun 2019 05:43:05: 4000000 INFO @ Mon, 03 Jun 2019 05:43:09: 6000000 INFO @ Mon, 03 Jun 2019 05:43:12: 5000000 INFO @ Mon, 03 Jun 2019 05:43:13: #1 tag size is determined as 100 bps INFO @ Mon, 03 Jun 2019 05:43:13: #1 tag size = 100 INFO @ Mon, 03 Jun 2019 05:43:13: #1 total tags in treatment: 6495532 INFO @ Mon, 03 Jun 2019 05:43:13: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:43:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:43:13: #1 tags after filtering in treatment: 6495532 INFO @ Mon, 03 Jun 2019 05:43:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:43:13: #1 finished! INFO @ Mon, 03 Jun 2019 05:43:13: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:43:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:43:15: #2 number of paired peaks: 6889 INFO @ Mon, 03 Jun 2019 05:43:15: start model_add_line... INFO @ Mon, 03 Jun 2019 05:43:15: start X-correlation... INFO @ Mon, 03 Jun 2019 05:43:15: end of X-cor INFO @ Mon, 03 Jun 2019 05:43:15: #2 finished! INFO @ Mon, 03 Jun 2019 05:43:15: #2 predicted fragment length is 126 bps INFO @ Mon, 03 Jun 2019 05:43:15: #2 alternative fragment length(s) may be 126 bps INFO @ Mon, 03 Jun 2019 05:43:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.05_model.r WARNING @ Mon, 03 Jun 2019 05:43:15: #2 Since the d (126) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 05:43:15: #2 You may need to consider one of the other alternative d(s): 126 WARNING @ Mon, 03 Jun 2019 05:43:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 05:43:15: #3 Call peaks... INFO @ Mon, 03 Jun 2019 05:43:15: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 05:43:15: 5000000 INFO @ Mon, 03 Jun 2019 05:43:22: 6000000 INFO @ Mon, 03 Jun 2019 05:43:25: 6000000 INFO @ Mon, 03 Jun 2019 05:43:28: #1 tag size is determined as 100 bps INFO @ Mon, 03 Jun 2019 05:43:28: #1 tag size = 100 INFO @ Mon, 03 Jun 2019 05:43:28: #1 total tags in treatment: 6495532 INFO @ Mon, 03 Jun 2019 05:43:28: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:43:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:43:28: #1 tags after filtering in treatment: 6495532 INFO @ Mon, 03 Jun 2019 05:43:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:43:28: #1 finished! INFO @ Mon, 03 Jun 2019 05:43:28: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:43:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:43:29: #2 number of paired peaks: 6889 INFO @ Mon, 03 Jun 2019 05:43:29: start model_add_line... INFO @ Mon, 03 Jun 2019 05:43:29: start X-correlation... INFO @ Mon, 03 Jun 2019 05:43:29: end of X-cor INFO @ Mon, 03 Jun 2019 05:43:29: #2 finished! INFO @ Mon, 03 Jun 2019 05:43:29: #2 predicted fragment length is 126 bps INFO @ Mon, 03 Jun 2019 05:43:29: #2 alternative fragment length(s) may be 126 bps INFO @ Mon, 03 Jun 2019 05:43:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.10_model.r WARNING @ Mon, 03 Jun 2019 05:43:29: #2 Since the d (126) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 05:43:29: #2 You may need to consider one of the other alternative d(s): 126 WARNING @ Mon, 03 Jun 2019 05:43:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 05:43:29: #3 Call peaks... INFO @ Mon, 03 Jun 2019 05:43:29: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 05:43:31: #1 tag size is determined as 100 bps INFO @ Mon, 03 Jun 2019 05:43:31: #1 tag size = 100 INFO @ Mon, 03 Jun 2019 05:43:31: #1 total tags in treatment: 6495532 INFO @ Mon, 03 Jun 2019 05:43:31: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:43:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:43:31: #1 tags after filtering in treatment: 6495532 INFO @ Mon, 03 Jun 2019 05:43:31: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:43:31: #1 finished! INFO @ Mon, 03 Jun 2019 05:43:31: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:43:31: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:43:32: #2 number of paired peaks: 6889 INFO @ Mon, 03 Jun 2019 05:43:32: start model_add_line... INFO @ Mon, 03 Jun 2019 05:43:32: start X-correlation... INFO @ Mon, 03 Jun 2019 05:43:32: end of X-cor INFO @ Mon, 03 Jun 2019 05:43:32: #2 finished! INFO @ Mon, 03 Jun 2019 05:43:32: #2 predicted fragment length is 126 bps INFO @ Mon, 03 Jun 2019 05:43:32: #2 alternative fragment length(s) may be 126 bps INFO @ Mon, 03 Jun 2019 05:43:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.20_model.r WARNING @ Mon, 03 Jun 2019 05:43:32: #2 Since the d (126) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 05:43:32: #2 You may need to consider one of the other alternative d(s): 126 WARNING @ Mon, 03 Jun 2019 05:43:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 05:43:32: #3 Call peaks... INFO @ Mon, 03 Jun 2019 05:43:32: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 05:43:36: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 05:43:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.05_peaks.xls INFO @ Mon, 03 Jun 2019 05:43:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 05:43:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.05_summits.bed INFO @ Mon, 03 Jun 2019 05:43:47: Done! pass1 - making usageList (14 chroms): 4 millis pass2 - checking and writing primary data (14059 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:43:51: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 05:43:55: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 05:44:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.10_peaks.xls INFO @ Mon, 03 Jun 2019 05:44:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 05:44:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.10_summits.bed INFO @ Mon, 03 Jun 2019 05:44:02: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (7547 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:44:05: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.20_peaks.xls INFO @ Mon, 03 Jun 2019 05:44:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 05:44:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX201882/SRX201882.20_summits.bed INFO @ Mon, 03 Jun 2019 05:44:05: Done! pass1 - making usageList (13 chroms): 3 millis pass2 - checking and writing primary data (2913 records, 4 fields): 10 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。