Job ID = 6527667 SRX = SRX2011089 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T12:49:57 prefetch.2.10.7: 1) Downloading 'SRR4017242'... 2020-06-29T12:49:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:53:28 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:53:28 prefetch.2.10.7: 1) 'SRR4017242' was downloaded successfully Read 15829804 spots for SRR4017242/SRR4017242.sra Written 15829804 spots for SRR4017242/SRR4017242.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:52 15829804 reads; of these: 15829804 (100.00%) were unpaired; of these: 594667 (3.76%) aligned 0 times 12279862 (77.57%) aligned exactly 1 time 2955275 (18.67%) aligned >1 times 96.24% overall alignment rate Time searching: 00:08:52 Overall time: 00:08:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 761173 / 15235137 = 0.0500 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:19:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:19:56: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:19:56: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:20:07: 1000000 INFO @ Mon, 29 Jun 2020 22:20:18: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:20:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:20:26: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:20:26: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:20:29: 3000000 INFO @ Mon, 29 Jun 2020 22:20:36: 1000000 INFO @ Mon, 29 Jun 2020 22:20:41: 4000000 INFO @ Mon, 29 Jun 2020 22:20:46: 2000000 INFO @ Mon, 29 Jun 2020 22:20:52: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:20:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:20:56: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:20:56: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:20:57: 3000000 INFO @ Mon, 29 Jun 2020 22:21:03: 6000000 INFO @ Mon, 29 Jun 2020 22:21:07: 1000000 INFO @ Mon, 29 Jun 2020 22:21:08: 4000000 INFO @ Mon, 29 Jun 2020 22:21:14: 7000000 INFO @ Mon, 29 Jun 2020 22:21:19: 2000000 INFO @ Mon, 29 Jun 2020 22:21:20: 5000000 INFO @ Mon, 29 Jun 2020 22:21:25: 8000000 INFO @ Mon, 29 Jun 2020 22:21:30: 3000000 INFO @ Mon, 29 Jun 2020 22:21:31: 6000000 INFO @ Mon, 29 Jun 2020 22:21:37: 9000000 INFO @ Mon, 29 Jun 2020 22:21:41: 4000000 INFO @ Mon, 29 Jun 2020 22:21:43: 7000000 INFO @ Mon, 29 Jun 2020 22:21:47: 10000000 INFO @ Mon, 29 Jun 2020 22:21:53: 5000000 INFO @ Mon, 29 Jun 2020 22:21:54: 8000000 INFO @ Mon, 29 Jun 2020 22:21:58: 11000000 INFO @ Mon, 29 Jun 2020 22:22:04: 6000000 INFO @ Mon, 29 Jun 2020 22:22:05: 9000000 INFO @ Mon, 29 Jun 2020 22:22:09: 12000000 INFO @ Mon, 29 Jun 2020 22:22:15: 7000000 INFO @ Mon, 29 Jun 2020 22:22:16: 10000000 INFO @ Mon, 29 Jun 2020 22:22:20: 13000000 INFO @ Mon, 29 Jun 2020 22:22:26: 8000000 INFO @ Mon, 29 Jun 2020 22:22:27: 11000000 INFO @ Mon, 29 Jun 2020 22:22:31: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:22:36: #1 tag size is determined as 100 bps INFO @ Mon, 29 Jun 2020 22:22:36: #1 tag size = 100 INFO @ Mon, 29 Jun 2020 22:22:36: #1 total tags in treatment: 14473964 INFO @ Mon, 29 Jun 2020 22:22:36: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:22:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:22:36: #1 tags after filtering in treatment: 14473964 INFO @ Mon, 29 Jun 2020 22:22:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:22:36: #1 finished! INFO @ Mon, 29 Jun 2020 22:22:36: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:22:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:22:37: #2 number of paired peaks: 31 WARNING @ Mon, 29 Jun 2020 22:22:37: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:22:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:22:38: 9000000 INFO @ Mon, 29 Jun 2020 22:22:38: 12000000 INFO @ Mon, 29 Jun 2020 22:22:48: 10000000 INFO @ Mon, 29 Jun 2020 22:22:49: 13000000 INFO @ Mon, 29 Jun 2020 22:22:59: 11000000 INFO @ Mon, 29 Jun 2020 22:22:59: 14000000 INFO @ Mon, 29 Jun 2020 22:23:04: #1 tag size is determined as 100 bps INFO @ Mon, 29 Jun 2020 22:23:04: #1 tag size = 100 INFO @ Mon, 29 Jun 2020 22:23:04: #1 total tags in treatment: 14473964 INFO @ Mon, 29 Jun 2020 22:23:04: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:23:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:23:05: #1 tags after filtering in treatment: 14473964 INFO @ Mon, 29 Jun 2020 22:23:05: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:23:05: #1 finished! INFO @ Mon, 29 Jun 2020 22:23:05: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:23:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:23:06: #2 number of paired peaks: 31 WARNING @ Mon, 29 Jun 2020 22:23:06: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:23:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:23:09: 12000000 BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 22:23:19: 13000000 INFO @ Mon, 29 Jun 2020 22:23:29: 14000000 INFO @ Mon, 29 Jun 2020 22:23:34: #1 tag size is determined as 100 bps INFO @ Mon, 29 Jun 2020 22:23:34: #1 tag size = 100 INFO @ Mon, 29 Jun 2020 22:23:34: #1 total tags in treatment: 14473964 INFO @ Mon, 29 Jun 2020 22:23:34: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:23:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:23:34: #1 tags after filtering in treatment: 14473964 INFO @ Mon, 29 Jun 2020 22:23:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:23:34: #1 finished! INFO @ Mon, 29 Jun 2020 22:23:34: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:23:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:23:35: #2 number of paired peaks: 31 WARNING @ Mon, 29 Jun 2020 22:23:35: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:23:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX2011089/SRX2011089.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling