Job ID = 1294200 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,350,273 reads read : 15,350,273 reads written : 15,350,273 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:00 15350273 reads; of these: 15350273 (100.00%) were unpaired; of these: 7286374 (47.47%) aligned 0 times 5431350 (35.38%) aligned exactly 1 time 2632549 (17.15%) aligned >1 times 52.53% overall alignment rate Time searching: 00:04:00 Overall time: 00:04:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 4722312 / 8063899 = 0.5856 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 05:31:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:31:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:31:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:31:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:31:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:31:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:31:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:31:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:31:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:31:49: 1000000 INFO @ Mon, 03 Jun 2019 05:31:50: 1000000 INFO @ Mon, 03 Jun 2019 05:31:50: 1000000 INFO @ Mon, 03 Jun 2019 05:31:57: 2000000 INFO @ Mon, 03 Jun 2019 05:31:58: 2000000 INFO @ Mon, 03 Jun 2019 05:31:59: 2000000 INFO @ Mon, 03 Jun 2019 05:32:04: 3000000 INFO @ Mon, 03 Jun 2019 05:32:06: #1 tag size is determined as 40 bps INFO @ Mon, 03 Jun 2019 05:32:06: #1 tag size = 40 INFO @ Mon, 03 Jun 2019 05:32:06: #1 total tags in treatment: 3341587 INFO @ Mon, 03 Jun 2019 05:32:06: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:32:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:32:06: #1 tags after filtering in treatment: 3341587 INFO @ Mon, 03 Jun 2019 05:32:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:32:06: #1 finished! INFO @ Mon, 03 Jun 2019 05:32:06: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:32:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:32:07: #2 number of paired peaks: 1024 INFO @ Mon, 03 Jun 2019 05:32:07: start model_add_line... INFO @ Mon, 03 Jun 2019 05:32:07: start X-correlation... INFO @ Mon, 03 Jun 2019 05:32:07: end of X-cor INFO @ Mon, 03 Jun 2019 05:32:07: #2 finished! INFO @ Mon, 03 Jun 2019 05:32:07: #2 predicted fragment length is 50 bps INFO @ Mon, 03 Jun 2019 05:32:07: #2 alternative fragment length(s) may be 50 bps INFO @ Mon, 03 Jun 2019 05:32:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.10_model.r WARNING @ Mon, 03 Jun 2019 05:32:07: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 05:32:07: #2 You may need to consider one of the other alternative d(s): 50 WARNING @ Mon, 03 Jun 2019 05:32:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 05:32:07: #3 Call peaks... INFO @ Mon, 03 Jun 2019 05:32:07: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 05:32:07: 3000000 INFO @ Mon, 03 Jun 2019 05:32:08: 3000000 INFO @ Mon, 03 Jun 2019 05:32:11: #1 tag size is determined as 40 bps INFO @ Mon, 03 Jun 2019 05:32:11: #1 tag size = 40 INFO @ Mon, 03 Jun 2019 05:32:11: #1 total tags in treatment: 3341587 INFO @ Mon, 03 Jun 2019 05:32:11: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:32:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:32:11: #1 tags after filtering in treatment: 3341587 INFO @ Mon, 03 Jun 2019 05:32:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:32:11: #1 finished! INFO @ Mon, 03 Jun 2019 05:32:11: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:32:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:32:11: #1 tag size is determined as 40 bps INFO @ Mon, 03 Jun 2019 05:32:11: #1 tag size = 40 INFO @ Mon, 03 Jun 2019 05:32:11: #1 total tags in treatment: 3341587 INFO @ Mon, 03 Jun 2019 05:32:11: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:32:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:32:11: #1 tags after filtering in treatment: 3341587 INFO @ Mon, 03 Jun 2019 05:32:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:32:11: #1 finished! INFO @ Mon, 03 Jun 2019 05:32:11: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:32:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:32:11: #2 number of paired peaks: 1024 INFO @ Mon, 03 Jun 2019 05:32:11: start model_add_line... INFO @ Mon, 03 Jun 2019 05:32:11: start X-correlation... INFO @ Mon, 03 Jun 2019 05:32:11: end of X-cor INFO @ Mon, 03 Jun 2019 05:32:11: #2 finished! INFO @ Mon, 03 Jun 2019 05:32:11: #2 predicted fragment length is 50 bps INFO @ Mon, 03 Jun 2019 05:32:11: #2 alternative fragment length(s) may be 50 bps INFO @ Mon, 03 Jun 2019 05:32:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.20_model.r WARNING @ Mon, 03 Jun 2019 05:32:11: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 05:32:11: #2 You may need to consider one of the other alternative d(s): 50 WARNING @ Mon, 03 Jun 2019 05:32:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 05:32:11: #3 Call peaks... INFO @ Mon, 03 Jun 2019 05:32:11: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 05:32:11: #2 number of paired peaks: 1024 INFO @ Mon, 03 Jun 2019 05:32:11: start model_add_line... INFO @ Mon, 03 Jun 2019 05:32:11: start X-correlation... INFO @ Mon, 03 Jun 2019 05:32:11: end of X-cor INFO @ Mon, 03 Jun 2019 05:32:11: #2 finished! INFO @ Mon, 03 Jun 2019 05:32:11: #2 predicted fragment length is 50 bps INFO @ Mon, 03 Jun 2019 05:32:11: #2 alternative fragment length(s) may be 50 bps INFO @ Mon, 03 Jun 2019 05:32:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.05_model.r WARNING @ Mon, 03 Jun 2019 05:32:11: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 05:32:11: #2 You may need to consider one of the other alternative d(s): 50 WARNING @ Mon, 03 Jun 2019 05:32:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 05:32:11: #3 Call peaks... INFO @ Mon, 03 Jun 2019 05:32:11: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 05:32:17: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 05:32:21: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 05:32:22: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 05:32:22: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.10_peaks.xls INFO @ Mon, 03 Jun 2019 05:32:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 05:32:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.10_summits.bed INFO @ Mon, 03 Jun 2019 05:32:22: Done! pass1 - making usageList (10 chroms): 2 millis pass2 - checking and writing primary data (1352 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:32:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.20_peaks.xls INFO @ Mon, 03 Jun 2019 05:32:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 05:32:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.20_summits.bed INFO @ Mon, 03 Jun 2019 05:32:26: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (850 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:32:27: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.05_peaks.xls INFO @ Mon, 03 Jun 2019 05:32:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 05:32:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX197584/SRX197584.05_summits.bed INFO @ Mon, 03 Jun 2019 05:32:27: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (1854 records, 4 fields): 8 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。