Job ID = 1294185 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 40,456,415 reads read : 40,456,415 reads written : 40,456,415 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:28 40456415 reads; of these: 40456415 (100.00%) were unpaired; of these: 1432479 (3.54%) aligned 0 times 27240782 (67.33%) aligned exactly 1 time 11783154 (29.13%) aligned >1 times 96.46% overall alignment rate Time searching: 00:14:28 Overall time: 00:14:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4418690 / 39023936 = 0.1132 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 05:48:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:48:03: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:48:03: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:48:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:48:03: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:48:03: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:48:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 05:48:03: #1 read tag files... INFO @ Mon, 03 Jun 2019 05:48:03: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 05:48:13: 1000000 INFO @ Mon, 03 Jun 2019 05:48:14: 1000000 INFO @ Mon, 03 Jun 2019 05:48:15: 1000000 INFO @ Mon, 03 Jun 2019 05:48:22: 2000000 INFO @ Mon, 03 Jun 2019 05:48:24: 2000000 INFO @ Mon, 03 Jun 2019 05:48:27: 2000000 INFO @ Mon, 03 Jun 2019 05:48:31: 3000000 INFO @ Mon, 03 Jun 2019 05:48:34: 3000000 INFO @ Mon, 03 Jun 2019 05:48:38: 3000000 INFO @ Mon, 03 Jun 2019 05:48:40: 4000000 INFO @ Mon, 03 Jun 2019 05:48:44: 4000000 INFO @ Mon, 03 Jun 2019 05:48:48: 4000000 INFO @ Mon, 03 Jun 2019 05:48:49: 5000000 INFO @ Mon, 03 Jun 2019 05:48:54: 5000000 INFO @ Mon, 03 Jun 2019 05:48:58: 6000000 INFO @ Mon, 03 Jun 2019 05:48:58: 5000000 INFO @ Mon, 03 Jun 2019 05:49:04: 6000000 INFO @ Mon, 03 Jun 2019 05:49:06: 7000000 INFO @ Mon, 03 Jun 2019 05:49:08: 6000000 INFO @ Mon, 03 Jun 2019 05:49:15: 7000000 INFO @ Mon, 03 Jun 2019 05:49:15: 8000000 INFO @ Mon, 03 Jun 2019 05:49:19: 7000000 INFO @ Mon, 03 Jun 2019 05:49:23: 9000000 INFO @ Mon, 03 Jun 2019 05:49:26: 8000000 INFO @ Mon, 03 Jun 2019 05:49:29: 8000000 INFO @ Mon, 03 Jun 2019 05:49:32: 10000000 INFO @ Mon, 03 Jun 2019 05:49:35: 9000000 INFO @ Mon, 03 Jun 2019 05:49:38: 9000000 INFO @ Mon, 03 Jun 2019 05:49:40: 11000000 INFO @ Mon, 03 Jun 2019 05:49:45: 10000000 INFO @ Mon, 03 Jun 2019 05:49:47: 10000000 INFO @ Mon, 03 Jun 2019 05:49:49: 12000000 INFO @ Mon, 03 Jun 2019 05:49:54: 11000000 INFO @ Mon, 03 Jun 2019 05:49:56: 11000000 INFO @ Mon, 03 Jun 2019 05:49:57: 13000000 INFO @ Mon, 03 Jun 2019 05:50:04: 12000000 INFO @ Mon, 03 Jun 2019 05:50:05: 12000000 INFO @ Mon, 03 Jun 2019 05:50:05: 14000000 INFO @ Mon, 03 Jun 2019 05:50:13: 13000000 INFO @ Mon, 03 Jun 2019 05:50:14: 15000000 INFO @ Mon, 03 Jun 2019 05:50:14: 13000000 INFO @ Mon, 03 Jun 2019 05:50:22: 16000000 INFO @ Mon, 03 Jun 2019 05:50:23: 14000000 INFO @ Mon, 03 Jun 2019 05:50:24: 14000000 INFO @ Mon, 03 Jun 2019 05:50:31: 17000000 INFO @ Mon, 03 Jun 2019 05:50:33: 15000000 INFO @ Mon, 03 Jun 2019 05:50:33: 15000000 INFO @ Mon, 03 Jun 2019 05:50:40: 18000000 INFO @ Mon, 03 Jun 2019 05:50:42: 16000000 INFO @ Mon, 03 Jun 2019 05:50:42: 16000000 INFO @ Mon, 03 Jun 2019 05:50:49: 19000000 INFO @ Mon, 03 Jun 2019 05:50:51: 17000000 INFO @ Mon, 03 Jun 2019 05:50:52: 17000000 INFO @ Mon, 03 Jun 2019 05:50:58: 20000000 INFO @ Mon, 03 Jun 2019 05:51:00: 18000000 INFO @ Mon, 03 Jun 2019 05:51:01: 18000000 INFO @ Mon, 03 Jun 2019 05:51:07: 21000000 INFO @ Mon, 03 Jun 2019 05:51:10: 19000000 INFO @ Mon, 03 Jun 2019 05:51:11: 19000000 INFO @ Mon, 03 Jun 2019 05:51:16: 22000000 INFO @ Mon, 03 Jun 2019 05:51:19: 20000000 INFO @ Mon, 03 Jun 2019 05:51:21: 20000000 INFO @ Mon, 03 Jun 2019 05:51:25: 23000000 INFO @ Mon, 03 Jun 2019 05:51:29: 21000000 INFO @ Mon, 03 Jun 2019 05:51:31: 21000000 INFO @ Mon, 03 Jun 2019 05:51:33: 24000000 INFO @ Mon, 03 Jun 2019 05:51:39: 22000000 INFO @ Mon, 03 Jun 2019 05:51:41: 22000000 INFO @ Mon, 03 Jun 2019 05:51:42: 25000000 INFO @ Mon, 03 Jun 2019 05:51:49: 23000000 INFO @ Mon, 03 Jun 2019 05:51:51: 26000000 INFO @ Mon, 03 Jun 2019 05:51:51: 23000000 INFO @ Mon, 03 Jun 2019 05:51:58: 24000000 INFO @ Mon, 03 Jun 2019 05:52:00: 27000000 INFO @ Mon, 03 Jun 2019 05:52:01: 24000000 INFO @ Mon, 03 Jun 2019 05:52:08: 25000000 INFO @ Mon, 03 Jun 2019 05:52:08: 28000000 INFO @ Mon, 03 Jun 2019 05:52:11: 25000000 INFO @ Mon, 03 Jun 2019 05:52:17: 29000000 INFO @ Mon, 03 Jun 2019 05:52:17: 26000000 INFO @ Mon, 03 Jun 2019 05:52:21: 26000000 INFO @ Mon, 03 Jun 2019 05:52:26: 30000000 INFO @ Mon, 03 Jun 2019 05:52:26: 27000000 INFO @ Mon, 03 Jun 2019 05:52:31: 27000000 INFO @ Mon, 03 Jun 2019 05:52:35: 31000000 INFO @ Mon, 03 Jun 2019 05:52:35: 28000000 INFO @ Mon, 03 Jun 2019 05:52:41: 28000000 INFO @ Mon, 03 Jun 2019 05:52:44: 29000000 INFO @ Mon, 03 Jun 2019 05:52:44: 32000000 INFO @ Mon, 03 Jun 2019 05:52:50: 29000000 INFO @ Mon, 03 Jun 2019 05:52:52: 30000000 INFO @ Mon, 03 Jun 2019 05:52:53: 33000000 INFO @ Mon, 03 Jun 2019 05:53:00: 30000000 INFO @ Mon, 03 Jun 2019 05:53:01: 31000000 INFO @ Mon, 03 Jun 2019 05:53:02: 34000000 INFO @ Mon, 03 Jun 2019 05:53:08: #1 tag size is determined as 40 bps INFO @ Mon, 03 Jun 2019 05:53:08: #1 tag size = 40 INFO @ Mon, 03 Jun 2019 05:53:08: #1 total tags in treatment: 34605246 INFO @ Mon, 03 Jun 2019 05:53:08: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:53:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:53:09: #1 tags after filtering in treatment: 34605246 INFO @ Mon, 03 Jun 2019 05:53:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:53:09: #1 finished! INFO @ Mon, 03 Jun 2019 05:53:09: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:53:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:53:09: 32000000 INFO @ Mon, 03 Jun 2019 05:53:10: 31000000 INFO @ Mon, 03 Jun 2019 05:53:11: #2 number of paired peaks: 57 WARNING @ Mon, 03 Jun 2019 05:53:11: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 05:53:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:53:18: 33000000 INFO @ Mon, 03 Jun 2019 05:53:20: 32000000 INFO @ Mon, 03 Jun 2019 05:53:27: 34000000 INFO @ Mon, 03 Jun 2019 05:53:30: 33000000 INFO @ Mon, 03 Jun 2019 05:53:32: #1 tag size is determined as 40 bps INFO @ Mon, 03 Jun 2019 05:53:32: #1 tag size = 40 INFO @ Mon, 03 Jun 2019 05:53:32: #1 total tags in treatment: 34605246 INFO @ Mon, 03 Jun 2019 05:53:32: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:53:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:53:33: #1 tags after filtering in treatment: 34605246 INFO @ Mon, 03 Jun 2019 05:53:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:53:33: #1 finished! INFO @ Mon, 03 Jun 2019 05:53:33: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:53:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:53:36: #2 number of paired peaks: 57 WARNING @ Mon, 03 Jun 2019 05:53:36: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 05:53:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 05:53:40: 34000000 INFO @ Mon, 03 Jun 2019 05:53:46: #1 tag size is determined as 40 bps INFO @ Mon, 03 Jun 2019 05:53:46: #1 tag size = 40 INFO @ Mon, 03 Jun 2019 05:53:46: #1 total tags in treatment: 34605246 INFO @ Mon, 03 Jun 2019 05:53:46: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 05:53:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 05:53:47: #1 tags after filtering in treatment: 34605246 INFO @ Mon, 03 Jun 2019 05:53:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 05:53:47: #1 finished! INFO @ Mon, 03 Jun 2019 05:53:47: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 05:53:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 05:53:49: #2 number of paired peaks: 57 WARNING @ Mon, 03 Jun 2019 05:53:49: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 05:53:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX197572/SRX197572.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。