Job ID = 6527653 SRX = SRX191915 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:09:36 prefetch.2.10.7: 1) Downloading 'SRR580345'... 2020-06-29T13:09:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:14:25 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:14:25 prefetch.2.10.7: 1) 'SRR580345' was downloaded successfully Read 32815353 spots for SRR580345/SRR580345.sra Written 32815353 spots for SRR580345/SRR580345.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:35 32815353 reads; of these: 32815353 (100.00%) were unpaired; of these: 925278 (2.82%) aligned 0 times 22157723 (67.52%) aligned exactly 1 time 9732352 (29.66%) aligned >1 times 97.18% overall alignment rate Time searching: 00:10:35 Overall time: 00:10:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6442883 / 31890075 = 0.2020 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:40:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:40:43: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:40:43: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:40:49: 1000000 INFO @ Mon, 29 Jun 2020 22:40:54: 2000000 INFO @ Mon, 29 Jun 2020 22:40:59: 3000000 INFO @ Mon, 29 Jun 2020 22:41:03: 4000000 INFO @ Mon, 29 Jun 2020 22:41:08: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:41:13: 6000000 INFO @ Mon, 29 Jun 2020 22:41:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:41:13: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:41:13: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:41:18: 1000000 INFO @ Mon, 29 Jun 2020 22:41:18: 7000000 INFO @ Mon, 29 Jun 2020 22:41:23: 2000000 INFO @ Mon, 29 Jun 2020 22:41:23: 8000000 INFO @ Mon, 29 Jun 2020 22:41:27: 3000000 INFO @ Mon, 29 Jun 2020 22:41:28: 9000000 INFO @ Mon, 29 Jun 2020 22:41:31: 4000000 INFO @ Mon, 29 Jun 2020 22:41:33: 10000000 INFO @ Mon, 29 Jun 2020 22:41:36: 5000000 INFO @ Mon, 29 Jun 2020 22:41:38: 11000000 INFO @ Mon, 29 Jun 2020 22:41:40: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:41:43: 12000000 INFO @ Mon, 29 Jun 2020 22:41:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:41:44: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:41:44: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:41:45: 7000000 INFO @ Mon, 29 Jun 2020 22:41:48: 1000000 INFO @ Mon, 29 Jun 2020 22:41:48: 13000000 INFO @ Mon, 29 Jun 2020 22:41:49: 8000000 INFO @ Mon, 29 Jun 2020 22:41:53: 2000000 INFO @ Mon, 29 Jun 2020 22:41:53: 14000000 INFO @ Mon, 29 Jun 2020 22:41:54: 9000000 INFO @ Mon, 29 Jun 2020 22:41:57: 3000000 INFO @ Mon, 29 Jun 2020 22:41:58: 10000000 INFO @ Mon, 29 Jun 2020 22:41:59: 15000000 INFO @ Mon, 29 Jun 2020 22:42:01: 4000000 INFO @ Mon, 29 Jun 2020 22:42:02: 11000000 INFO @ Mon, 29 Jun 2020 22:42:04: 16000000 INFO @ Mon, 29 Jun 2020 22:42:06: 5000000 INFO @ Mon, 29 Jun 2020 22:42:07: 12000000 INFO @ Mon, 29 Jun 2020 22:42:09: 17000000 INFO @ Mon, 29 Jun 2020 22:42:10: 6000000 INFO @ Mon, 29 Jun 2020 22:42:11: 13000000 INFO @ Mon, 29 Jun 2020 22:42:14: 18000000 INFO @ Mon, 29 Jun 2020 22:42:15: 7000000 INFO @ Mon, 29 Jun 2020 22:42:16: 14000000 INFO @ Mon, 29 Jun 2020 22:42:19: 19000000 INFO @ Mon, 29 Jun 2020 22:42:19: 8000000 INFO @ Mon, 29 Jun 2020 22:42:20: 15000000 INFO @ Mon, 29 Jun 2020 22:42:24: 9000000 INFO @ Mon, 29 Jun 2020 22:42:24: 20000000 INFO @ Mon, 29 Jun 2020 22:42:25: 16000000 INFO @ Mon, 29 Jun 2020 22:42:28: 10000000 INFO @ Mon, 29 Jun 2020 22:42:29: 17000000 INFO @ Mon, 29 Jun 2020 22:42:30: 21000000 INFO @ Mon, 29 Jun 2020 22:42:32: 11000000 INFO @ Mon, 29 Jun 2020 22:42:34: 18000000 INFO @ Mon, 29 Jun 2020 22:42:35: 22000000 INFO @ Mon, 29 Jun 2020 22:42:37: 12000000 INFO @ Mon, 29 Jun 2020 22:42:38: 19000000 INFO @ Mon, 29 Jun 2020 22:42:40: 23000000 INFO @ Mon, 29 Jun 2020 22:42:41: 13000000 INFO @ Mon, 29 Jun 2020 22:42:43: 20000000 INFO @ Mon, 29 Jun 2020 22:42:45: 24000000 INFO @ Mon, 29 Jun 2020 22:42:46: 14000000 INFO @ Mon, 29 Jun 2020 22:42:47: 21000000 INFO @ Mon, 29 Jun 2020 22:42:50: 25000000 INFO @ Mon, 29 Jun 2020 22:42:50: 15000000 INFO @ Mon, 29 Jun 2020 22:42:52: 22000000 INFO @ Mon, 29 Jun 2020 22:42:53: #1 tag size is determined as 36 bps INFO @ Mon, 29 Jun 2020 22:42:53: #1 tag size = 36 INFO @ Mon, 29 Jun 2020 22:42:53: #1 total tags in treatment: 25447192 INFO @ Mon, 29 Jun 2020 22:42:53: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:42:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:42:53: #1 tags after filtering in treatment: 25447192 INFO @ Mon, 29 Jun 2020 22:42:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:42:53: #1 finished! INFO @ Mon, 29 Jun 2020 22:42:53: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:42:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:42:55: #2 number of paired peaks: 42 WARNING @ Mon, 29 Jun 2020 22:42:55: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:42:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:42:55: 16000000 INFO @ Mon, 29 Jun 2020 22:42:56: 23000000 INFO @ Mon, 29 Jun 2020 22:42:59: 17000000 INFO @ Mon, 29 Jun 2020 22:43:00: 24000000 INFO @ Mon, 29 Jun 2020 22:43:04: 18000000 INFO @ Mon, 29 Jun 2020 22:43:05: 25000000 INFO @ Mon, 29 Jun 2020 22:43:07: #1 tag size is determined as 36 bps INFO @ Mon, 29 Jun 2020 22:43:07: #1 tag size = 36 INFO @ Mon, 29 Jun 2020 22:43:07: #1 total tags in treatment: 25447192 INFO @ Mon, 29 Jun 2020 22:43:07: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:43:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:43:07: #1 tags after filtering in treatment: 25447192 INFO @ Mon, 29 Jun 2020 22:43:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:43:07: #1 finished! INFO @ Mon, 29 Jun 2020 22:43:07: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:43:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:43:08: 19000000 INFO @ Mon, 29 Jun 2020 22:43:09: #2 number of paired peaks: 42 WARNING @ Mon, 29 Jun 2020 22:43:09: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:43:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:43:13: 20000000 INFO @ Mon, 29 Jun 2020 22:43:17: 21000000 INFO @ Mon, 29 Jun 2020 22:43:22: 22000000 INFO @ Mon, 29 Jun 2020 22:43:26: 23000000 INFO @ Mon, 29 Jun 2020 22:43:31: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:43:35: 25000000 INFO @ Mon, 29 Jun 2020 22:43:37: #1 tag size is determined as 36 bps INFO @ Mon, 29 Jun 2020 22:43:37: #1 tag size = 36 INFO @ Mon, 29 Jun 2020 22:43:37: #1 total tags in treatment: 25447192 INFO @ Mon, 29 Jun 2020 22:43:37: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:43:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:43:38: #1 tags after filtering in treatment: 25447192 INFO @ Mon, 29 Jun 2020 22:43:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:43:38: #1 finished! INFO @ Mon, 29 Jun 2020 22:43:38: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:43:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:43:39: #2 number of paired peaks: 42 WARNING @ Mon, 29 Jun 2020 22:43:39: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:43:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX191915/SRX191915.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。