Job ID = 9158617 sra ファイルのダウンロード中... Completed: 1672539K bytes transferred in 16 seconds (827340K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 51140598 spots for /home/okishinya/chipatlas/results/dm3/SRX1872294/SRR3712755.sra Written 51140598 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:21 51140598 reads; of these: 51140598 (100.00%) were unpaired; of these: 3879331 (7.59%) aligned 0 times 43037119 (84.15%) aligned exactly 1 time 4224148 (8.26%) aligned >1 times 92.41% overall alignment rate Time searching: 00:15:21 Overall time: 00:15:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12089361 / 47261267 = 0.2558 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 19:01:11: # Command line: callpeak -t SRX1872294.bam -f BAM -g dm -n SRX1872294.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1872294.05 # format = BAM # ChIP-seq file = ['SRX1872294.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:01:11: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:01:11: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:01:11: # Command line: callpeak -t SRX1872294.bam -f BAM -g dm -n SRX1872294.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1872294.10 # format = BAM # ChIP-seq file = ['SRX1872294.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:01:11: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:01:11: # Command line: callpeak -t SRX1872294.bam -f BAM -g dm -n SRX1872294.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1872294.20 # format = BAM # ChIP-seq file = ['SRX1872294.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:01:11: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:01:11: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:01:11: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:01:18: 1000000 INFO @ Tue, 27 Jun 2017 19:01:21: 1000000 INFO @ Tue, 27 Jun 2017 19:01:21: 1000000 INFO @ Tue, 27 Jun 2017 19:01:25: 2000000 INFO @ Tue, 27 Jun 2017 19:01:31: 2000000 INFO @ Tue, 27 Jun 2017 19:01:31: 2000000 INFO @ Tue, 27 Jun 2017 19:01:33: 3000000 INFO @ Tue, 27 Jun 2017 19:01:39: 4000000 INFO @ Tue, 27 Jun 2017 19:01:41: 3000000 INFO @ Tue, 27 Jun 2017 19:01:41: 3000000 INFO @ Tue, 27 Jun 2017 19:01:47: 5000000 INFO @ Tue, 27 Jun 2017 19:01:51: 4000000 INFO @ Tue, 27 Jun 2017 19:01:51: 4000000 INFO @ Tue, 27 Jun 2017 19:01:54: 6000000 INFO @ Tue, 27 Jun 2017 19:02:01: 7000000 INFO @ Tue, 27 Jun 2017 19:02:01: 5000000 INFO @ Tue, 27 Jun 2017 19:02:01: 5000000 INFO @ Tue, 27 Jun 2017 19:02:08: 8000000 INFO @ Tue, 27 Jun 2017 19:02:10: 6000000 INFO @ Tue, 27 Jun 2017 19:02:11: 6000000 INFO @ Tue, 27 Jun 2017 19:02:15: 9000000 INFO @ Tue, 27 Jun 2017 19:02:20: 7000000 INFO @ Tue, 27 Jun 2017 19:02:20: 7000000 INFO @ Tue, 27 Jun 2017 19:02:22: 10000000 INFO @ Tue, 27 Jun 2017 19:02:29: 11000000 INFO @ Tue, 27 Jun 2017 19:02:30: 8000000 INFO @ Tue, 27 Jun 2017 19:02:30: 8000000 INFO @ Tue, 27 Jun 2017 19:02:36: 12000000 INFO @ Tue, 27 Jun 2017 19:02:40: 9000000 INFO @ Tue, 27 Jun 2017 19:02:40: 9000000 INFO @ Tue, 27 Jun 2017 19:02:43: 13000000 INFO @ Tue, 27 Jun 2017 19:02:50: 10000000 INFO @ Tue, 27 Jun 2017 19:02:50: 14000000 INFO @ Tue, 27 Jun 2017 19:02:50: 10000000 INFO @ Tue, 27 Jun 2017 19:02:57: 15000000 INFO @ Tue, 27 Jun 2017 19:02:59: 11000000 INFO @ Tue, 27 Jun 2017 19:03:00: 11000000 INFO @ Tue, 27 Jun 2017 19:03:04: 16000000 INFO @ Tue, 27 Jun 2017 19:03:09: 12000000 INFO @ Tue, 27 Jun 2017 19:03:09: 12000000 INFO @ Tue, 27 Jun 2017 19:03:11: 17000000 INFO @ Tue, 27 Jun 2017 19:03:18: 18000000 INFO @ Tue, 27 Jun 2017 19:03:19: 13000000 INFO @ Tue, 27 Jun 2017 19:03:19: 13000000 INFO @ Tue, 27 Jun 2017 19:03:26: 19000000 INFO @ Tue, 27 Jun 2017 19:03:29: 14000000 INFO @ Tue, 27 Jun 2017 19:03:29: 14000000 INFO @ Tue, 27 Jun 2017 19:03:34: 20000000 INFO @ Tue, 27 Jun 2017 19:03:39: 15000000 INFO @ Tue, 27 Jun 2017 19:03:39: 15000000 INFO @ Tue, 27 Jun 2017 19:03:42: 21000000 INFO @ Tue, 27 Jun 2017 19:03:49: 16000000 INFO @ Tue, 27 Jun 2017 19:03:49: 16000000 INFO @ Tue, 27 Jun 2017 19:03:51: 22000000 INFO @ Tue, 27 Jun 2017 19:03:59: 17000000 INFO @ Tue, 27 Jun 2017 19:03:59: 17000000 INFO @ Tue, 27 Jun 2017 19:03:59: 23000000 INFO @ Tue, 27 Jun 2017 19:04:07: 18000000 INFO @ Tue, 27 Jun 2017 19:04:07: 24000000 INFO @ Tue, 27 Jun 2017 19:04:07: 18000000 INFO @ Tue, 27 Jun 2017 19:04:15: 25000000 INFO @ Tue, 27 Jun 2017 19:04:15: 19000000 INFO @ Tue, 27 Jun 2017 19:04:15: 19000000 INFO @ Tue, 27 Jun 2017 19:04:24: 26000000 INFO @ Tue, 27 Jun 2017 19:04:24: 20000000 INFO @ Tue, 27 Jun 2017 19:04:24: 20000000 INFO @ Tue, 27 Jun 2017 19:04:32: 27000000 INFO @ Tue, 27 Jun 2017 19:04:32: 21000000 INFO @ Tue, 27 Jun 2017 19:04:32: 21000000 INFO @ Tue, 27 Jun 2017 19:04:40: 28000000 INFO @ Tue, 27 Jun 2017 19:04:41: 22000000 INFO @ Tue, 27 Jun 2017 19:04:41: 22000000 INFO @ Tue, 27 Jun 2017 19:04:49: 23000000 INFO @ Tue, 27 Jun 2017 19:04:49: 29000000 INFO @ Tue, 27 Jun 2017 19:04:49: 23000000 INFO @ Tue, 27 Jun 2017 19:04:58: 24000000 INFO @ Tue, 27 Jun 2017 19:04:58: 24000000 INFO @ Tue, 27 Jun 2017 19:04:59: 30000000 INFO @ Tue, 27 Jun 2017 19:05:07: 25000000 INFO @ Tue, 27 Jun 2017 19:05:07: 25000000 INFO @ Tue, 27 Jun 2017 19:05:09: 31000000 INFO @ Tue, 27 Jun 2017 19:05:15: 26000000 INFO @ Tue, 27 Jun 2017 19:05:16: 26000000 INFO @ Tue, 27 Jun 2017 19:05:18: 32000000 INFO @ Tue, 27 Jun 2017 19:05:24: 27000000 INFO @ Tue, 27 Jun 2017 19:05:24: 27000000 INFO @ Tue, 27 Jun 2017 19:05:28: 33000000 INFO @ Tue, 27 Jun 2017 19:05:33: 28000000 INFO @ Tue, 27 Jun 2017 19:05:33: 28000000 INFO @ Tue, 27 Jun 2017 19:05:37: 34000000 INFO @ Tue, 27 Jun 2017 19:05:42: 29000000 INFO @ Tue, 27 Jun 2017 19:05:42: 29000000 INFO @ Tue, 27 Jun 2017 19:05:47: 35000000 INFO @ Tue, 27 Jun 2017 19:05:48: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 19:05:48: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 19:05:48: #1 total tags in treatment: 35171906 INFO @ Tue, 27 Jun 2017 19:05:48: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:05:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:05:49: #1 tags after filtering in treatment: 35171906 INFO @ Tue, 27 Jun 2017 19:05:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:05:49: #1 finished! INFO @ Tue, 27 Jun 2017 19:05:49: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:05:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:05:50: 30000000 INFO @ Tue, 27 Jun 2017 19:05:50: 30000000 INFO @ Tue, 27 Jun 2017 19:05:51: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 19:05:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:05:51: Process for pairing-model is terminated! cat: SRX1872294.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1872294.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1872294.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1872294.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:05:58: 31000000 INFO @ Tue, 27 Jun 2017 19:05:58: 31000000 INFO @ Tue, 27 Jun 2017 19:06:05: 32000000 INFO @ Tue, 27 Jun 2017 19:06:05: 32000000 INFO @ Tue, 27 Jun 2017 19:06:13: 33000000 INFO @ Tue, 27 Jun 2017 19:06:13: 33000000 INFO @ Tue, 27 Jun 2017 19:06:21: 34000000 INFO @ Tue, 27 Jun 2017 19:06:21: 34000000 INFO @ Tue, 27 Jun 2017 19:06:28: 35000000 INFO @ Tue, 27 Jun 2017 19:06:28: 35000000 INFO @ Tue, 27 Jun 2017 19:06:30: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 19:06:30: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 19:06:30: #1 total tags in treatment: 35171906 INFO @ Tue, 27 Jun 2017 19:06:30: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:06:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:06:30: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 19:06:30: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 19:06:30: #1 total tags in treatment: 35171906 INFO @ Tue, 27 Jun 2017 19:06:30: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:06:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:06:31: #1 tags after filtering in treatment: 35171906 INFO @ Tue, 27 Jun 2017 19:06:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:06:31: #1 finished! INFO @ Tue, 27 Jun 2017 19:06:31: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:06:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:06:31: #1 tags after filtering in treatment: 35171906 INFO @ Tue, 27 Jun 2017 19:06:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:06:31: #1 finished! INFO @ Tue, 27 Jun 2017 19:06:31: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:06:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:06:33: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 19:06:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:06:33: Process for pairing-model is terminated! cat: SRX1872294.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1872294.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1872294.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1872294.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:06:33: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 19:06:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:06:33: Process for pairing-model is terminated! cat: SRX1872294.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1872294.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1872294.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1872294.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。