Job ID = 9158615 sra ファイルのダウンロード中... Completed: 1087397K bytes transferred in 13 seconds (670428K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 33449159 spots for /home/okishinya/chipatlas/results/dm3/SRX1872293/SRR3712754.sra Written 33449159 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:30 33449159 reads; of these: 33449159 (100.00%) were unpaired; of these: 2181987 (6.52%) aligned 0 times 27152120 (81.17%) aligned exactly 1 time 4115052 (12.30%) aligned >1 times 93.48% overall alignment rate Time searching: 00:10:31 Overall time: 00:10:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5205078 / 31267172 = 0.1665 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 18:51:41: # Command line: callpeak -t SRX1872293.bam -f BAM -g dm -n SRX1872293.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1872293.05 # format = BAM # ChIP-seq file = ['SRX1872293.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:51:41: # Command line: callpeak -t SRX1872293.bam -f BAM -g dm -n SRX1872293.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1872293.20 # format = BAM # ChIP-seq file = ['SRX1872293.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:51:41: # Command line: callpeak -t SRX1872293.bam -f BAM -g dm -n SRX1872293.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1872293.10 # format = BAM # ChIP-seq file = ['SRX1872293.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:51:41: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:51:41: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:51:41: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:51:41: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:51:41: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:51:41: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:51:49: 1000000 INFO @ Tue, 27 Jun 2017 18:51:49: 1000000 INFO @ Tue, 27 Jun 2017 18:51:50: 1000000 INFO @ Tue, 27 Jun 2017 18:51:58: 2000000 INFO @ Tue, 27 Jun 2017 18:51:58: 2000000 INFO @ Tue, 27 Jun 2017 18:51:59: 2000000 INFO @ Tue, 27 Jun 2017 18:52:06: 3000000 INFO @ Tue, 27 Jun 2017 18:52:06: 3000000 INFO @ Tue, 27 Jun 2017 18:52:08: 3000000 INFO @ Tue, 27 Jun 2017 18:52:14: 4000000 INFO @ Tue, 27 Jun 2017 18:52:14: 4000000 INFO @ Tue, 27 Jun 2017 18:52:17: 4000000 INFO @ Tue, 27 Jun 2017 18:52:22: 5000000 INFO @ Tue, 27 Jun 2017 18:52:22: 5000000 INFO @ Tue, 27 Jun 2017 18:52:26: 5000000 INFO @ Tue, 27 Jun 2017 18:52:31: 6000000 INFO @ Tue, 27 Jun 2017 18:52:31: 6000000 INFO @ Tue, 27 Jun 2017 18:52:35: 6000000 INFO @ Tue, 27 Jun 2017 18:52:39: 7000000 INFO @ Tue, 27 Jun 2017 18:52:39: 7000000 INFO @ Tue, 27 Jun 2017 18:52:44: 7000000 INFO @ Tue, 27 Jun 2017 18:52:47: 8000000 INFO @ Tue, 27 Jun 2017 18:52:47: 8000000 INFO @ Tue, 27 Jun 2017 18:52:53: 8000000 INFO @ Tue, 27 Jun 2017 18:52:55: 9000000 INFO @ Tue, 27 Jun 2017 18:52:55: 9000000 INFO @ Tue, 27 Jun 2017 18:53:02: 9000000 INFO @ Tue, 27 Jun 2017 18:53:03: 10000000 INFO @ Tue, 27 Jun 2017 18:53:03: 10000000 INFO @ Tue, 27 Jun 2017 18:53:11: 10000000 INFO @ Tue, 27 Jun 2017 18:53:12: 11000000 INFO @ Tue, 27 Jun 2017 18:53:12: 11000000 INFO @ Tue, 27 Jun 2017 18:53:20: 12000000 INFO @ Tue, 27 Jun 2017 18:53:20: 12000000 INFO @ Tue, 27 Jun 2017 18:53:20: 11000000 INFO @ Tue, 27 Jun 2017 18:53:28: 13000000 INFO @ Tue, 27 Jun 2017 18:53:28: 13000000 INFO @ Tue, 27 Jun 2017 18:53:29: 12000000 INFO @ Tue, 27 Jun 2017 18:53:36: 14000000 INFO @ Tue, 27 Jun 2017 18:53:36: 14000000 INFO @ Tue, 27 Jun 2017 18:53:38: 13000000 INFO @ Tue, 27 Jun 2017 18:53:44: 15000000 INFO @ Tue, 27 Jun 2017 18:53:44: 15000000 INFO @ Tue, 27 Jun 2017 18:53:47: 14000000 INFO @ Tue, 27 Jun 2017 18:53:52: 16000000 INFO @ Tue, 27 Jun 2017 18:53:52: 16000000 INFO @ Tue, 27 Jun 2017 18:53:56: 15000000 INFO @ Tue, 27 Jun 2017 18:54:01: 17000000 INFO @ Tue, 27 Jun 2017 18:54:01: 17000000 INFO @ Tue, 27 Jun 2017 18:54:05: 16000000 INFO @ Tue, 27 Jun 2017 18:54:09: 18000000 INFO @ Tue, 27 Jun 2017 18:54:09: 18000000 INFO @ Tue, 27 Jun 2017 18:54:14: 17000000 INFO @ Tue, 27 Jun 2017 18:54:17: 19000000 INFO @ Tue, 27 Jun 2017 18:54:17: 19000000 INFO @ Tue, 27 Jun 2017 18:54:23: 18000000 INFO @ Tue, 27 Jun 2017 18:54:26: 20000000 INFO @ Tue, 27 Jun 2017 18:54:26: 20000000 INFO @ Tue, 27 Jun 2017 18:54:32: 19000000 INFO @ Tue, 27 Jun 2017 18:54:34: 21000000 INFO @ Tue, 27 Jun 2017 18:54:34: 21000000 INFO @ Tue, 27 Jun 2017 18:54:41: 20000000 INFO @ Tue, 27 Jun 2017 18:54:42: 22000000 INFO @ Tue, 27 Jun 2017 18:54:42: 22000000 INFO @ Tue, 27 Jun 2017 18:54:51: 21000000 INFO @ Tue, 27 Jun 2017 18:54:51: 23000000 INFO @ Tue, 27 Jun 2017 18:54:51: 23000000 INFO @ Tue, 27 Jun 2017 18:54:59: 24000000 INFO @ Tue, 27 Jun 2017 18:54:59: 24000000 INFO @ Tue, 27 Jun 2017 18:55:00: 22000000 INFO @ Tue, 27 Jun 2017 18:55:08: 25000000 INFO @ Tue, 27 Jun 2017 18:55:08: 25000000 INFO @ Tue, 27 Jun 2017 18:55:10: 23000000 INFO @ Tue, 27 Jun 2017 18:55:16: 26000000 INFO @ Tue, 27 Jun 2017 18:55:16: 26000000 INFO @ Tue, 27 Jun 2017 18:55:17: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 18:55:17: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 18:55:17: #1 total tags in treatment: 26062094 INFO @ Tue, 27 Jun 2017 18:55:17: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:55:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:55:17: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 18:55:17: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 18:55:17: #1 total tags in treatment: 26062094 INFO @ Tue, 27 Jun 2017 18:55:17: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:55:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:55:17: #1 tags after filtering in treatment: 26062094 INFO @ Tue, 27 Jun 2017 18:55:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:55:17: #1 finished! INFO @ Tue, 27 Jun 2017 18:55:17: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:55:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:55:17: #1 tags after filtering in treatment: 26062094 INFO @ Tue, 27 Jun 2017 18:55:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:55:17: #1 finished! INFO @ Tue, 27 Jun 2017 18:55:17: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:55:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:55:18: 24000000 INFO @ Tue, 27 Jun 2017 18:55:19: #2 number of paired peaks: 32 WARNING @ Tue, 27 Jun 2017 18:55:19: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:55:19: Process for pairing-model is terminated! cat: SRX1872293.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1872293.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1872293.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1872293.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:55:19: #2 number of paired peaks: 32 WARNING @ Tue, 27 Jun 2017 18:55:19: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:55:19: Process for pairing-model is terminated! cat: SRX1872293.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1872293.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1872293.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1872293.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:55:25: 25000000 INFO @ Tue, 27 Jun 2017 18:55:32: 26000000 INFO @ Tue, 27 Jun 2017 18:55:33: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 18:55:33: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 18:55:33: #1 total tags in treatment: 26062094 INFO @ Tue, 27 Jun 2017 18:55:33: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:55:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:55:33: #1 tags after filtering in treatment: 26062094 INFO @ Tue, 27 Jun 2017 18:55:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:55:33: #1 finished! INFO @ Tue, 27 Jun 2017 18:55:33: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:55:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:55:35: #2 number of paired peaks: 32 WARNING @ Tue, 27 Jun 2017 18:55:35: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:55:35: Process for pairing-model is terminated! cat: SRX1872293.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1872293.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1872293.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1872293.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。