Job ID = 1294112 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,300,219 reads read : 6,300,219 reads written : 6,300,219 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:41 6300219 reads; of these: 6300219 (100.00%) were unpaired; of these: 238697 (3.79%) aligned 0 times 4230280 (67.14%) aligned exactly 1 time 1831242 (29.07%) aligned >1 times 96.21% overall alignment rate Time searching: 00:02:41 Overall time: 00:02:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 2407813 / 6061522 = 0.3972 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 04:56:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:56:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:56:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:56:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:56:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:56:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:56:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:56:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:56:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:56:50: 1000000 INFO @ Mon, 03 Jun 2019 04:56:51: 1000000 INFO @ Mon, 03 Jun 2019 04:56:52: 1000000 INFO @ Mon, 03 Jun 2019 04:56:57: 2000000 INFO @ Mon, 03 Jun 2019 04:56:59: 2000000 INFO @ Mon, 03 Jun 2019 04:57:01: 2000000 INFO @ Mon, 03 Jun 2019 04:57:04: 3000000 INFO @ Mon, 03 Jun 2019 04:57:07: 3000000 INFO @ Mon, 03 Jun 2019 04:57:09: #1 tag size is determined as 36 bps INFO @ Mon, 03 Jun 2019 04:57:09: #1 tag size = 36 INFO @ Mon, 03 Jun 2019 04:57:09: #1 total tags in treatment: 3653709 INFO @ Mon, 03 Jun 2019 04:57:09: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:57:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:57:09: #1 tags after filtering in treatment: 3653709 INFO @ Mon, 03 Jun 2019 04:57:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:57:09: #1 finished! INFO @ Mon, 03 Jun 2019 04:57:09: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:57:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:57:10: #2 number of paired peaks: 4649 INFO @ Mon, 03 Jun 2019 04:57:10: start model_add_line... INFO @ Mon, 03 Jun 2019 04:57:10: start X-correlation... INFO @ Mon, 03 Jun 2019 04:57:10: end of X-cor INFO @ Mon, 03 Jun 2019 04:57:10: #2 finished! INFO @ Mon, 03 Jun 2019 04:57:10: #2 predicted fragment length is 98 bps INFO @ Mon, 03 Jun 2019 04:57:10: #2 alternative fragment length(s) may be 98 bps INFO @ Mon, 03 Jun 2019 04:57:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.10_model.r INFO @ Mon, 03 Jun 2019 04:57:10: #3 Call peaks... INFO @ Mon, 03 Jun 2019 04:57:10: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 04:57:11: 3000000 INFO @ Mon, 03 Jun 2019 04:57:12: #1 tag size is determined as 36 bps INFO @ Mon, 03 Jun 2019 04:57:12: #1 tag size = 36 INFO @ Mon, 03 Jun 2019 04:57:12: #1 total tags in treatment: 3653709 INFO @ Mon, 03 Jun 2019 04:57:12: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:57:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:57:12: #1 tags after filtering in treatment: 3653709 INFO @ Mon, 03 Jun 2019 04:57:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:57:12: #1 finished! INFO @ Mon, 03 Jun 2019 04:57:12: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:57:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:57:12: #2 number of paired peaks: 4649 INFO @ Mon, 03 Jun 2019 04:57:12: start model_add_line... INFO @ Mon, 03 Jun 2019 04:57:12: start X-correlation... INFO @ Mon, 03 Jun 2019 04:57:12: end of X-cor INFO @ Mon, 03 Jun 2019 04:57:12: #2 finished! INFO @ Mon, 03 Jun 2019 04:57:12: #2 predicted fragment length is 98 bps INFO @ Mon, 03 Jun 2019 04:57:12: #2 alternative fragment length(s) may be 98 bps INFO @ Mon, 03 Jun 2019 04:57:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.20_model.r INFO @ Mon, 03 Jun 2019 04:57:12: #3 Call peaks... INFO @ Mon, 03 Jun 2019 04:57:12: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 04:57:16: #1 tag size is determined as 36 bps INFO @ Mon, 03 Jun 2019 04:57:16: #1 tag size = 36 INFO @ Mon, 03 Jun 2019 04:57:16: #1 total tags in treatment: 3653709 INFO @ Mon, 03 Jun 2019 04:57:16: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:57:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:57:17: #1 tags after filtering in treatment: 3653709 INFO @ Mon, 03 Jun 2019 04:57:17: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:57:17: #1 finished! INFO @ Mon, 03 Jun 2019 04:57:17: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:57:17: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:57:17: #2 number of paired peaks: 4649 INFO @ Mon, 03 Jun 2019 04:57:17: start model_add_line... INFO @ Mon, 03 Jun 2019 04:57:17: start X-correlation... INFO @ Mon, 03 Jun 2019 04:57:17: end of X-cor INFO @ Mon, 03 Jun 2019 04:57:17: #2 finished! INFO @ Mon, 03 Jun 2019 04:57:17: #2 predicted fragment length is 98 bps INFO @ Mon, 03 Jun 2019 04:57:17: #2 alternative fragment length(s) may be 98 bps INFO @ Mon, 03 Jun 2019 04:57:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.05_model.r INFO @ Mon, 03 Jun 2019 04:57:17: #3 Call peaks... INFO @ Mon, 03 Jun 2019 04:57:17: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 04:57:21: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 04:57:24: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 04:57:27: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.10_peaks.xls INFO @ Mon, 03 Jun 2019 04:57:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 04:57:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.10_summits.bed INFO @ Mon, 03 Jun 2019 04:57:27: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (7577 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:57:28: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 04:57:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.20_peaks.xls INFO @ Mon, 03 Jun 2019 04:57:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 04:57:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.20_summits.bed INFO @ Mon, 03 Jun 2019 04:57:30: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (3193 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:57:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.05_peaks.xls INFO @ Mon, 03 Jun 2019 04:57:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 04:57:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX187082/SRX187082.05_summits.bed INFO @ Mon, 03 Jun 2019 04:57:34: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (11892 records, 4 fields): 16 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。