Job ID = 9158600 sra ファイルのダウンロード中... Completed: 1287280K bytes transferred in 14 seconds (726352K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 31013148 spots for /home/okishinya/chipatlas/results/dm3/SRX1847044/SRR3669861.sra Written 31013148 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:31 31013148 reads; of these: 31013148 (100.00%) were unpaired; of these: 685810 (2.21%) aligned 0 times 26808516 (86.44%) aligned exactly 1 time 3518822 (11.35%) aligned >1 times 97.79% overall alignment rate Time searching: 00:09:31 Overall time: 00:09:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 9209992 / 30327338 = 0.3037 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 18:44:27: # Command line: callpeak -t SRX1847044.bam -f BAM -g dm -n SRX1847044.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1847044.05 # format = BAM # ChIP-seq file = ['SRX1847044.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:44:27: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:44:27: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:44:27: # Command line: callpeak -t SRX1847044.bam -f BAM -g dm -n SRX1847044.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1847044.20 # format = BAM # ChIP-seq file = ['SRX1847044.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:44:27: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:44:27: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:44:27: # Command line: callpeak -t SRX1847044.bam -f BAM -g dm -n SRX1847044.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1847044.10 # format = BAM # ChIP-seq file = ['SRX1847044.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 18:44:27: #1 read tag files... INFO @ Tue, 27 Jun 2017 18:44:27: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 18:44:35: 1000000 INFO @ Tue, 27 Jun 2017 18:44:35: 1000000 INFO @ Tue, 27 Jun 2017 18:44:35: 1000000 INFO @ Tue, 27 Jun 2017 18:44:41: 2000000 INFO @ Tue, 27 Jun 2017 18:44:42: 2000000 INFO @ Tue, 27 Jun 2017 18:44:42: 2000000 INFO @ Tue, 27 Jun 2017 18:44:48: 3000000 INFO @ Tue, 27 Jun 2017 18:44:49: 3000000 INFO @ Tue, 27 Jun 2017 18:44:49: 3000000 INFO @ Tue, 27 Jun 2017 18:44:55: 4000000 INFO @ Tue, 27 Jun 2017 18:44:56: 4000000 INFO @ Tue, 27 Jun 2017 18:44:56: 4000000 INFO @ Tue, 27 Jun 2017 18:45:02: 5000000 INFO @ Tue, 27 Jun 2017 18:45:03: 5000000 INFO @ Tue, 27 Jun 2017 18:45:03: 5000000 INFO @ Tue, 27 Jun 2017 18:45:09: 6000000 INFO @ Tue, 27 Jun 2017 18:45:11: 6000000 INFO @ Tue, 27 Jun 2017 18:45:11: 6000000 INFO @ Tue, 27 Jun 2017 18:45:16: 7000000 INFO @ Tue, 27 Jun 2017 18:45:18: 7000000 INFO @ Tue, 27 Jun 2017 18:45:18: 7000000 INFO @ Tue, 27 Jun 2017 18:45:23: 8000000 INFO @ Tue, 27 Jun 2017 18:45:25: 8000000 INFO @ Tue, 27 Jun 2017 18:45:25: 8000000 INFO @ Tue, 27 Jun 2017 18:45:30: 9000000 INFO @ Tue, 27 Jun 2017 18:45:32: 9000000 INFO @ Tue, 27 Jun 2017 18:45:32: 9000000 INFO @ Tue, 27 Jun 2017 18:45:37: 10000000 INFO @ Tue, 27 Jun 2017 18:45:40: 10000000 INFO @ Tue, 27 Jun 2017 18:45:40: 10000000 INFO @ Tue, 27 Jun 2017 18:45:44: 11000000 INFO @ Tue, 27 Jun 2017 18:45:47: 11000000 INFO @ Tue, 27 Jun 2017 18:45:48: 11000000 INFO @ Tue, 27 Jun 2017 18:45:53: 12000000 INFO @ Tue, 27 Jun 2017 18:45:55: 12000000 INFO @ Tue, 27 Jun 2017 18:45:56: 12000000 INFO @ Tue, 27 Jun 2017 18:46:02: 13000000 INFO @ Tue, 27 Jun 2017 18:46:03: 13000000 INFO @ Tue, 27 Jun 2017 18:46:04: 13000000 INFO @ Tue, 27 Jun 2017 18:46:10: 14000000 INFO @ Tue, 27 Jun 2017 18:46:11: 14000000 INFO @ Tue, 27 Jun 2017 18:46:11: 14000000 INFO @ Tue, 27 Jun 2017 18:46:16: 15000000 INFO @ Tue, 27 Jun 2017 18:46:18: 15000000 INFO @ Tue, 27 Jun 2017 18:46:18: 15000000 INFO @ Tue, 27 Jun 2017 18:46:23: 16000000 INFO @ Tue, 27 Jun 2017 18:46:26: 16000000 INFO @ Tue, 27 Jun 2017 18:46:26: 16000000 INFO @ Tue, 27 Jun 2017 18:46:30: 17000000 INFO @ Tue, 27 Jun 2017 18:46:33: 17000000 INFO @ Tue, 27 Jun 2017 18:46:33: 17000000 INFO @ Tue, 27 Jun 2017 18:46:37: 18000000 INFO @ Tue, 27 Jun 2017 18:46:40: 18000000 INFO @ Tue, 27 Jun 2017 18:46:40: 18000000 INFO @ Tue, 27 Jun 2017 18:46:43: 19000000 INFO @ Tue, 27 Jun 2017 18:46:47: 19000000 INFO @ Tue, 27 Jun 2017 18:46:48: 19000000 INFO @ Tue, 27 Jun 2017 18:46:50: 20000000 INFO @ Tue, 27 Jun 2017 18:46:54: 20000000 INFO @ Tue, 27 Jun 2017 18:46:55: 20000000 INFO @ Tue, 27 Jun 2017 18:46:57: 21000000 INFO @ Tue, 27 Jun 2017 18:46:58: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:46:58: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:46:58: #1 total tags in treatment: 21117346 INFO @ Tue, 27 Jun 2017 18:46:58: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:46:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:46:58: #1 tags after filtering in treatment: 21117346 INFO @ Tue, 27 Jun 2017 18:46:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:46:58: #1 finished! INFO @ Tue, 27 Jun 2017 18:46:58: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:46:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:47:00: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 18:47:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:47:00: Process for pairing-model is terminated! cat: SRX1847044.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1847044.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847044.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847044.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:47:02: 21000000 INFO @ Tue, 27 Jun 2017 18:47:02: 21000000 INFO @ Tue, 27 Jun 2017 18:47:02: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:47:02: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:47:02: #1 total tags in treatment: 21117346 INFO @ Tue, 27 Jun 2017 18:47:02: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:47:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:47:03: #1 tag size is determined as 49 bps INFO @ Tue, 27 Jun 2017 18:47:03: #1 tag size = 49 INFO @ Tue, 27 Jun 2017 18:47:03: #1 total tags in treatment: 21117346 INFO @ Tue, 27 Jun 2017 18:47:03: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 18:47:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 18:47:03: #1 tags after filtering in treatment: 21117346 INFO @ Tue, 27 Jun 2017 18:47:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:47:03: #1 finished! INFO @ Tue, 27 Jun 2017 18:47:03: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:47:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:47:03: #1 tags after filtering in treatment: 21117346 INFO @ Tue, 27 Jun 2017 18:47:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 18:47:03: #1 finished! INFO @ Tue, 27 Jun 2017 18:47:03: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 18:47:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 18:47:04: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 18:47:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:47:04: Process for pairing-model is terminated! cat: SRX1847044.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1847044.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847044.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847044.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 18:47:04: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 18:47:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 18:47:04: Process for pairing-model is terminated! cat: SRX1847044.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1847044.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847044.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1847044.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。