Job ID = 6527647 SRX = SRX1840077 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:40:41 prefetch.2.10.7: 1) Downloading 'SRR3661303'... 2020-06-29T13:40:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:53:37 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:53:37 prefetch.2.10.7: 1) 'SRR3661303' was downloaded successfully Read 45897715 spots for SRR3661303/SRR3661303.sra Written 45897715 spots for SRR3661303/SRR3661303.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:38:24 45897715 reads; of these: 45897715 (100.00%) were unpaired; of these: 1061470 (2.31%) aligned 0 times 29768051 (64.86%) aligned exactly 1 time 15068194 (32.83%) aligned >1 times 97.69% overall alignment rate Time searching: 00:38:24 Overall time: 00:38:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8115719 / 44836245 = 0.1810 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:32:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:32:04: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:32:04: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:32:15: 1000000 INFO @ Tue, 30 Jun 2020 00:32:26: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:32:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:32:34: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:32:34: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:32:37: 3000000 INFO @ Tue, 30 Jun 2020 00:32:42: 1000000 INFO @ Tue, 30 Jun 2020 00:32:47: 4000000 INFO @ Tue, 30 Jun 2020 00:32:51: 2000000 INFO @ Tue, 30 Jun 2020 00:32:57: 5000000 INFO @ Tue, 30 Jun 2020 00:32:59: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:33:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:33:04: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:33:04: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:33:07: 6000000 INFO @ Tue, 30 Jun 2020 00:33:08: 4000000 INFO @ Tue, 30 Jun 2020 00:33:14: 1000000 INFO @ Tue, 30 Jun 2020 00:33:17: 5000000 INFO @ Tue, 30 Jun 2020 00:33:18: 7000000 INFO @ Tue, 30 Jun 2020 00:33:25: 2000000 INFO @ Tue, 30 Jun 2020 00:33:26: 6000000 INFO @ Tue, 30 Jun 2020 00:33:28: 8000000 INFO @ Tue, 30 Jun 2020 00:33:34: 7000000 INFO @ Tue, 30 Jun 2020 00:33:35: 3000000 INFO @ Tue, 30 Jun 2020 00:33:38: 9000000 INFO @ Tue, 30 Jun 2020 00:33:42: 8000000 INFO @ Tue, 30 Jun 2020 00:33:45: 4000000 INFO @ Tue, 30 Jun 2020 00:33:48: 10000000 INFO @ Tue, 30 Jun 2020 00:33:51: 9000000 INFO @ Tue, 30 Jun 2020 00:33:54: 5000000 INFO @ Tue, 30 Jun 2020 00:33:58: 11000000 INFO @ Tue, 30 Jun 2020 00:33:59: 10000000 INFO @ Tue, 30 Jun 2020 00:34:04: 6000000 INFO @ Tue, 30 Jun 2020 00:34:06: 12000000 INFO @ Tue, 30 Jun 2020 00:34:07: 11000000 INFO @ Tue, 30 Jun 2020 00:34:12: 7000000 INFO @ Tue, 30 Jun 2020 00:34:15: 13000000 INFO @ Tue, 30 Jun 2020 00:34:15: 12000000 INFO @ Tue, 30 Jun 2020 00:34:21: 8000000 INFO @ Tue, 30 Jun 2020 00:34:23: 13000000 INFO @ Tue, 30 Jun 2020 00:34:23: 14000000 INFO @ Tue, 30 Jun 2020 00:34:29: 9000000 INFO @ Tue, 30 Jun 2020 00:34:31: 14000000 INFO @ Tue, 30 Jun 2020 00:34:32: 15000000 INFO @ Tue, 30 Jun 2020 00:34:38: 10000000 INFO @ Tue, 30 Jun 2020 00:34:40: 15000000 INFO @ Tue, 30 Jun 2020 00:34:41: 16000000 INFO @ Tue, 30 Jun 2020 00:34:46: 11000000 INFO @ Tue, 30 Jun 2020 00:34:48: 16000000 INFO @ Tue, 30 Jun 2020 00:34:49: 17000000 INFO @ Tue, 30 Jun 2020 00:34:55: 12000000 INFO @ Tue, 30 Jun 2020 00:34:56: 17000000 INFO @ Tue, 30 Jun 2020 00:34:58: 18000000 INFO @ Tue, 30 Jun 2020 00:35:03: 13000000 INFO @ Tue, 30 Jun 2020 00:35:04: 18000000 INFO @ Tue, 30 Jun 2020 00:35:06: 19000000 INFO @ Tue, 30 Jun 2020 00:35:11: 14000000 INFO @ Tue, 30 Jun 2020 00:35:12: 19000000 INFO @ Tue, 30 Jun 2020 00:35:14: 20000000 INFO @ Tue, 30 Jun 2020 00:35:20: 15000000 INFO @ Tue, 30 Jun 2020 00:35:20: 20000000 INFO @ Tue, 30 Jun 2020 00:35:23: 21000000 INFO @ Tue, 30 Jun 2020 00:35:28: 16000000 INFO @ Tue, 30 Jun 2020 00:35:28: 21000000 INFO @ Tue, 30 Jun 2020 00:35:31: 22000000 INFO @ Tue, 30 Jun 2020 00:35:37: 22000000 INFO @ Tue, 30 Jun 2020 00:35:37: 17000000 INFO @ Tue, 30 Jun 2020 00:35:40: 23000000 INFO @ Tue, 30 Jun 2020 00:35:45: 18000000 INFO @ Tue, 30 Jun 2020 00:35:46: 23000000 INFO @ Tue, 30 Jun 2020 00:35:48: 24000000 INFO @ Tue, 30 Jun 2020 00:35:54: 24000000 INFO @ Tue, 30 Jun 2020 00:35:54: 19000000 INFO @ Tue, 30 Jun 2020 00:35:57: 25000000 INFO @ Tue, 30 Jun 2020 00:36:02: 20000000 INFO @ Tue, 30 Jun 2020 00:36:03: 25000000 INFO @ Tue, 30 Jun 2020 00:36:05: 26000000 INFO @ Tue, 30 Jun 2020 00:36:11: 21000000 INFO @ Tue, 30 Jun 2020 00:36:11: 26000000 INFO @ Tue, 30 Jun 2020 00:36:14: 27000000 INFO @ Tue, 30 Jun 2020 00:36:20: 22000000 INFO @ Tue, 30 Jun 2020 00:36:20: 27000000 INFO @ Tue, 30 Jun 2020 00:36:23: 28000000 INFO @ Tue, 30 Jun 2020 00:36:28: 28000000 INFO @ Tue, 30 Jun 2020 00:36:29: 23000000 INFO @ Tue, 30 Jun 2020 00:36:32: 29000000 INFO @ Tue, 30 Jun 2020 00:36:36: 29000000 INFO @ Tue, 30 Jun 2020 00:36:38: 24000000 INFO @ Tue, 30 Jun 2020 00:36:42: 30000000 INFO @ Tue, 30 Jun 2020 00:36:44: 30000000 INFO @ Tue, 30 Jun 2020 00:36:48: 25000000 INFO @ Tue, 30 Jun 2020 00:36:51: 31000000 INFO @ Tue, 30 Jun 2020 00:36:53: 31000000 INFO @ Tue, 30 Jun 2020 00:36:57: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:37:01: 32000000 INFO @ Tue, 30 Jun 2020 00:37:02: 32000000 INFO @ Tue, 30 Jun 2020 00:37:07: 27000000 INFO @ Tue, 30 Jun 2020 00:37:11: 33000000 INFO @ Tue, 30 Jun 2020 00:37:12: 33000000 INFO @ Tue, 30 Jun 2020 00:37:17: 28000000 INFO @ Tue, 30 Jun 2020 00:37:21: 34000000 INFO @ Tue, 30 Jun 2020 00:37:22: 34000000 INFO @ Tue, 30 Jun 2020 00:37:27: 29000000 INFO @ Tue, 30 Jun 2020 00:37:32: 35000000 INFO @ Tue, 30 Jun 2020 00:37:32: 35000000 INFO @ Tue, 30 Jun 2020 00:37:37: 30000000 INFO @ Tue, 30 Jun 2020 00:37:42: 36000000 INFO @ Tue, 30 Jun 2020 00:37:42: 36000000 INFO @ Tue, 30 Jun 2020 00:37:47: 31000000 INFO @ Tue, 30 Jun 2020 00:37:50: #1 tag size is determined as 101 bps INFO @ Tue, 30 Jun 2020 00:37:50: #1 tag size = 101 INFO @ Tue, 30 Jun 2020 00:37:50: #1 total tags in treatment: 36720526 INFO @ Tue, 30 Jun 2020 00:37:50: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:37:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:37:50: #1 tag size is determined as 101 bps INFO @ Tue, 30 Jun 2020 00:37:50: #1 tag size = 101 INFO @ Tue, 30 Jun 2020 00:37:50: #1 total tags in treatment: 36720526 INFO @ Tue, 30 Jun 2020 00:37:50: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:37:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:37:51: #1 tags after filtering in treatment: 36720526 INFO @ Tue, 30 Jun 2020 00:37:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:37:51: #1 finished! INFO @ Tue, 30 Jun 2020 00:37:51: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:37:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:37:51: #1 tags after filtering in treatment: 36720526 INFO @ Tue, 30 Jun 2020 00:37:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:37:51: #1 finished! INFO @ Tue, 30 Jun 2020 00:37:51: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:37:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:37:53: #2 number of paired peaks: 7 WARNING @ Tue, 30 Jun 2020 00:37:53: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:37:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:37:53: #2 number of paired peaks: 7 WARNING @ Tue, 30 Jun 2020 00:37:53: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:37:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:37:56: 32000000 INFO @ Tue, 30 Jun 2020 00:38:06: 33000000 INFO @ Tue, 30 Jun 2020 00:38:16: 34000000 INFO @ Tue, 30 Jun 2020 00:38:26: 35000000 INFO @ Tue, 30 Jun 2020 00:38:37: 36000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:38:43: #1 tag size is determined as 101 bps INFO @ Tue, 30 Jun 2020 00:38:43: #1 tag size = 101 INFO @ Tue, 30 Jun 2020 00:38:43: #1 total tags in treatment: 36720526 INFO @ Tue, 30 Jun 2020 00:38:43: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:38:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:38:44: #1 tags after filtering in treatment: 36720526 INFO @ Tue, 30 Jun 2020 00:38:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:38:44: #1 finished! INFO @ Tue, 30 Jun 2020 00:38:44: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:38:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:38:47: #2 number of paired peaks: 7 WARNING @ Tue, 30 Jun 2020 00:38:47: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:38:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1840077/SRX1840077.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling