Job ID = 1294017 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,078,964 reads read : 10,157,928 reads written : 5,078,964 reads 0-length : 5,078,964 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:19 5078964 reads; of these: 5078964 (100.00%) were unpaired; of these: 1611494 (31.73%) aligned 0 times 2553481 (50.28%) aligned exactly 1 time 913989 (18.00%) aligned >1 times 68.27% overall alignment rate Time searching: 00:08:19 Overall time: 00:08:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 134875 / 3467470 = 0.0389 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 04:22:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:22:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:22:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:22:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:22:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:22:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:22:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 04:22:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 04:22:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 04:23:08: 1000000 INFO @ Mon, 03 Jun 2019 04:23:10: 1000000 INFO @ Mon, 03 Jun 2019 04:23:13: 1000000 INFO @ Mon, 03 Jun 2019 04:23:23: 2000000 INFO @ Mon, 03 Jun 2019 04:23:27: 2000000 INFO @ Mon, 03 Jun 2019 04:23:32: 2000000 INFO @ Mon, 03 Jun 2019 04:23:37: 3000000 INFO @ Mon, 03 Jun 2019 04:23:42: #1 tag size is determined as 151 bps INFO @ Mon, 03 Jun 2019 04:23:42: #1 tag size = 151 INFO @ Mon, 03 Jun 2019 04:23:42: #1 total tags in treatment: 3332595 INFO @ Mon, 03 Jun 2019 04:23:42: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:23:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:23:42: #1 tags after filtering in treatment: 3332595 INFO @ Mon, 03 Jun 2019 04:23:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:23:42: #1 finished! INFO @ Mon, 03 Jun 2019 04:23:42: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:23:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:23:42: #2 number of paired peaks: 265 WARNING @ Mon, 03 Jun 2019 04:23:42: Fewer paired peaks (265) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 265 pairs to build model! INFO @ Mon, 03 Jun 2019 04:23:42: start model_add_line... INFO @ Mon, 03 Jun 2019 04:23:42: start X-correlation... INFO @ Mon, 03 Jun 2019 04:23:42: end of X-cor INFO @ Mon, 03 Jun 2019 04:23:42: #2 finished! INFO @ Mon, 03 Jun 2019 04:23:42: #2 predicted fragment length is 140 bps INFO @ Mon, 03 Jun 2019 04:23:42: #2 alternative fragment length(s) may be 140 bps INFO @ Mon, 03 Jun 2019 04:23:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.10_model.r WARNING @ Mon, 03 Jun 2019 04:23:42: #2 Since the d (140) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 04:23:42: #2 You may need to consider one of the other alternative d(s): 140 WARNING @ Mon, 03 Jun 2019 04:23:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 04:23:42: #3 Call peaks... INFO @ Mon, 03 Jun 2019 04:23:42: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 04:23:43: 3000000 INFO @ Mon, 03 Jun 2019 04:23:48: #1 tag size is determined as 151 bps INFO @ Mon, 03 Jun 2019 04:23:48: #1 tag size = 151 INFO @ Mon, 03 Jun 2019 04:23:48: #1 total tags in treatment: 3332595 INFO @ Mon, 03 Jun 2019 04:23:48: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:23:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:23:48: #1 tags after filtering in treatment: 3332595 INFO @ Mon, 03 Jun 2019 04:23:48: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:23:48: #1 finished! INFO @ Mon, 03 Jun 2019 04:23:48: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:23:48: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:23:49: #2 number of paired peaks: 265 WARNING @ Mon, 03 Jun 2019 04:23:49: Fewer paired peaks (265) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 265 pairs to build model! INFO @ Mon, 03 Jun 2019 04:23:49: start model_add_line... INFO @ Mon, 03 Jun 2019 04:23:49: start X-correlation... INFO @ Mon, 03 Jun 2019 04:23:49: end of X-cor INFO @ Mon, 03 Jun 2019 04:23:49: #2 finished! INFO @ Mon, 03 Jun 2019 04:23:49: #2 predicted fragment length is 140 bps INFO @ Mon, 03 Jun 2019 04:23:49: #2 alternative fragment length(s) may be 140 bps INFO @ Mon, 03 Jun 2019 04:23:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.05_model.r WARNING @ Mon, 03 Jun 2019 04:23:49: #2 Since the d (140) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 04:23:49: #2 You may need to consider one of the other alternative d(s): 140 WARNING @ Mon, 03 Jun 2019 04:23:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 04:23:49: #3 Call peaks... INFO @ Mon, 03 Jun 2019 04:23:49: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 04:23:51: 3000000 INFO @ Mon, 03 Jun 2019 04:23:54: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 04:23:57: #1 tag size is determined as 151 bps INFO @ Mon, 03 Jun 2019 04:23:57: #1 tag size = 151 INFO @ Mon, 03 Jun 2019 04:23:57: #1 total tags in treatment: 3332595 INFO @ Mon, 03 Jun 2019 04:23:57: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 04:23:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 04:23:57: #1 tags after filtering in treatment: 3332595 INFO @ Mon, 03 Jun 2019 04:23:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 04:23:57: #1 finished! INFO @ Mon, 03 Jun 2019 04:23:57: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 04:23:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 04:23:57: #2 number of paired peaks: 265 WARNING @ Mon, 03 Jun 2019 04:23:57: Fewer paired peaks (265) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 265 pairs to build model! INFO @ Mon, 03 Jun 2019 04:23:57: start model_add_line... INFO @ Mon, 03 Jun 2019 04:23:57: start X-correlation... INFO @ Mon, 03 Jun 2019 04:23:57: end of X-cor INFO @ Mon, 03 Jun 2019 04:23:57: #2 finished! INFO @ Mon, 03 Jun 2019 04:23:57: #2 predicted fragment length is 140 bps INFO @ Mon, 03 Jun 2019 04:23:57: #2 alternative fragment length(s) may be 140 bps INFO @ Mon, 03 Jun 2019 04:23:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.20_model.r WARNING @ Mon, 03 Jun 2019 04:23:57: #2 Since the d (140) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 04:23:57: #2 You may need to consider one of the other alternative d(s): 140 WARNING @ Mon, 03 Jun 2019 04:23:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 04:23:57: #3 Call peaks... INFO @ Mon, 03 Jun 2019 04:23:57: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 04:23:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.10_peaks.xls INFO @ Mon, 03 Jun 2019 04:23:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 04:23:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.10_summits.bed INFO @ Mon, 03 Jun 2019 04:23:59: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (325 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:24:00: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 04:24:06: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.05_peaks.xls INFO @ Mon, 03 Jun 2019 04:24:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 04:24:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.05_summits.bed INFO @ Mon, 03 Jun 2019 04:24:06: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (548 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 04:24:08: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 04:24:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.20_peaks.xls INFO @ Mon, 03 Jun 2019 04:24:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 04:24:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX1760695/SRX1760695.20_summits.bed INFO @ Mon, 03 Jun 2019 04:24:13: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (165 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。