Job ID = 9158528 sra ファイルのダウンロード中... Completed: 1998663K bytes transferred in 19 seconds (826255K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 36549214 spots for /home/okishinya/chipatlas/results/dm3/SRX1600286/SRR3187709.sra Written 36549214 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:44 36549214 reads; of these: 36549214 (100.00%) were unpaired; of these: 5316771 (14.55%) aligned 0 times 24864015 (68.03%) aligned exactly 1 time 6368428 (17.42%) aligned >1 times 85.45% overall alignment rate Time searching: 00:16:44 Overall time: 00:16:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7336761 / 31232443 = 0.2349 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 17:54:04: # Command line: callpeak -t SRX1600286.bam -f BAM -g dm -n SRX1600286.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1600286.05 # format = BAM # ChIP-seq file = ['SRX1600286.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:54:04: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:54:04: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:54:04: # Command line: callpeak -t SRX1600286.bam -f BAM -g dm -n SRX1600286.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1600286.10 # format = BAM # ChIP-seq file = ['SRX1600286.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:54:04: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:54:04: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:54:04: # Command line: callpeak -t SRX1600286.bam -f BAM -g dm -n SRX1600286.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1600286.20 # format = BAM # ChIP-seq file = ['SRX1600286.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:54:04: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:54:04: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:54:14: 1000000 INFO @ Tue, 27 Jun 2017 17:54:15: 1000000 INFO @ Tue, 27 Jun 2017 17:54:16: 1000000 INFO @ Tue, 27 Jun 2017 17:54:23: 2000000 INFO @ Tue, 27 Jun 2017 17:54:27: 2000000 INFO @ Tue, 27 Jun 2017 17:54:28: 2000000 INFO @ Tue, 27 Jun 2017 17:54:31: 3000000 INFO @ Tue, 27 Jun 2017 17:54:38: 3000000 INFO @ Tue, 27 Jun 2017 17:54:39: 3000000 INFO @ Tue, 27 Jun 2017 17:54:39: 4000000 INFO @ Tue, 27 Jun 2017 17:54:48: 5000000 INFO @ Tue, 27 Jun 2017 17:54:49: 4000000 INFO @ Tue, 27 Jun 2017 17:54:51: 4000000 INFO @ Tue, 27 Jun 2017 17:54:57: 6000000 INFO @ Tue, 27 Jun 2017 17:55:01: 5000000 INFO @ Tue, 27 Jun 2017 17:55:03: 5000000 INFO @ Tue, 27 Jun 2017 17:55:07: 7000000 INFO @ Tue, 27 Jun 2017 17:55:13: 6000000 INFO @ Tue, 27 Jun 2017 17:55:15: 8000000 INFO @ Tue, 27 Jun 2017 17:55:16: 6000000 INFO @ Tue, 27 Jun 2017 17:55:24: 9000000 INFO @ Tue, 27 Jun 2017 17:55:27: 7000000 INFO @ Tue, 27 Jun 2017 17:55:28: 7000000 INFO @ Tue, 27 Jun 2017 17:55:32: 10000000 INFO @ Tue, 27 Jun 2017 17:55:39: 8000000 INFO @ Tue, 27 Jun 2017 17:55:40: 11000000 INFO @ Tue, 27 Jun 2017 17:55:41: 8000000 INFO @ Tue, 27 Jun 2017 17:55:48: 12000000 INFO @ Tue, 27 Jun 2017 17:55:52: 9000000 INFO @ Tue, 27 Jun 2017 17:55:52: 9000000 INFO @ Tue, 27 Jun 2017 17:55:56: 13000000 INFO @ Tue, 27 Jun 2017 17:56:03: 10000000 INFO @ Tue, 27 Jun 2017 17:56:04: 10000000 INFO @ Tue, 27 Jun 2017 17:56:05: 14000000 INFO @ Tue, 27 Jun 2017 17:56:13: 15000000 INFO @ Tue, 27 Jun 2017 17:56:14: 11000000 INFO @ Tue, 27 Jun 2017 17:56:16: 11000000 INFO @ Tue, 27 Jun 2017 17:56:21: 16000000 INFO @ Tue, 27 Jun 2017 17:56:26: 12000000 INFO @ Tue, 27 Jun 2017 17:56:28: 12000000 INFO @ Tue, 27 Jun 2017 17:56:29: 17000000 INFO @ Tue, 27 Jun 2017 17:56:38: 18000000 INFO @ Tue, 27 Jun 2017 17:56:39: 13000000 INFO @ Tue, 27 Jun 2017 17:56:39: 13000000 INFO @ Tue, 27 Jun 2017 17:56:46: 19000000 INFO @ Tue, 27 Jun 2017 17:56:50: 14000000 INFO @ Tue, 27 Jun 2017 17:56:51: 14000000 INFO @ Tue, 27 Jun 2017 17:56:54: 20000000 INFO @ Tue, 27 Jun 2017 17:57:02: 15000000 INFO @ Tue, 27 Jun 2017 17:57:02: 21000000 INFO @ Tue, 27 Jun 2017 17:57:03: 15000000 INFO @ Tue, 27 Jun 2017 17:57:10: 22000000 INFO @ Tue, 27 Jun 2017 17:57:13: 16000000 INFO @ Tue, 27 Jun 2017 17:57:14: 16000000 INFO @ Tue, 27 Jun 2017 17:57:18: 23000000 INFO @ Tue, 27 Jun 2017 17:57:25: 17000000 INFO @ Tue, 27 Jun 2017 17:57:26: #1 tag size is determined as 76 bps INFO @ Tue, 27 Jun 2017 17:57:26: #1 tag size = 76 INFO @ Tue, 27 Jun 2017 17:57:26: #1 total tags in treatment: 23895682 INFO @ Tue, 27 Jun 2017 17:57:26: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:57:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:57:27: #1 tags after filtering in treatment: 23895682 INFO @ Tue, 27 Jun 2017 17:57:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:57:27: #1 finished! INFO @ Tue, 27 Jun 2017 17:57:27: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:57:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:57:27: 17000000 INFO @ Tue, 27 Jun 2017 17:57:29: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 17:57:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:57:29: Process for pairing-model is terminated! cat: SRX1600286.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1600286.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1600286.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1600286.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:57:35: 18000000 INFO @ Tue, 27 Jun 2017 17:57:37: 18000000 INFO @ Tue, 27 Jun 2017 17:57:45: 19000000 INFO @ Tue, 27 Jun 2017 17:57:47: 19000000 INFO @ Tue, 27 Jun 2017 17:57:55: 20000000 INFO @ Tue, 27 Jun 2017 17:57:57: 20000000 INFO @ Tue, 27 Jun 2017 17:58:05: 21000000 INFO @ Tue, 27 Jun 2017 17:58:07: 21000000 INFO @ Tue, 27 Jun 2017 17:58:15: 22000000 INFO @ Tue, 27 Jun 2017 17:58:17: 22000000 INFO @ Tue, 27 Jun 2017 17:58:25: 23000000 INFO @ Tue, 27 Jun 2017 17:58:27: 23000000 INFO @ Tue, 27 Jun 2017 17:58:34: #1 tag size is determined as 76 bps INFO @ Tue, 27 Jun 2017 17:58:34: #1 tag size = 76 INFO @ Tue, 27 Jun 2017 17:58:34: #1 total tags in treatment: 23895682 INFO @ Tue, 27 Jun 2017 17:58:34: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:58:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:58:34: #1 tags after filtering in treatment: 23895682 INFO @ Tue, 27 Jun 2017 17:58:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:58:34: #1 finished! INFO @ Tue, 27 Jun 2017 17:58:34: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:58:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:58:36: #1 tag size is determined as 76 bps INFO @ Tue, 27 Jun 2017 17:58:36: #1 tag size = 76 INFO @ Tue, 27 Jun 2017 17:58:36: #1 total tags in treatment: 23895682 INFO @ Tue, 27 Jun 2017 17:58:36: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:58:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:58:36: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 17:58:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:58:36: Process for pairing-model is terminated! cat: SRX1600286.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1600286.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1600286.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1600286.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:58:36: #1 tags after filtering in treatment: 23895682 INFO @ Tue, 27 Jun 2017 17:58:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:58:36: #1 finished! INFO @ Tue, 27 Jun 2017 17:58:36: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:58:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:58:38: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 17:58:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:58:38: Process for pairing-model is terminated! cat: SRX1600286.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1600286.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1600286.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1600286.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。