Job ID = 6527626 SRX = SRX159167 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T12:41:31 prefetch.2.10.7: 1) Downloading 'SRR520448'... 2020-06-29T12:41:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:49:29 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:49:29 prefetch.2.10.7: 1) 'SRR520448' was downloaded successfully Read 69003930 spots for SRR520448/SRR520448.sra Written 69003930 spots for SRR520448/SRR520448.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:54 69003930 reads; of these: 69003930 (100.00%) were unpaired; of these: 9854236 (14.28%) aligned 0 times 42813004 (62.04%) aligned exactly 1 time 16336690 (23.68%) aligned >1 times 85.72% overall alignment rate Time searching: 00:22:54 Overall time: 00:22:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 17855242 / 59149694 = 0.3019 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:42:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:42:16: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:42:16: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:42:21: 1000000 INFO @ Mon, 29 Jun 2020 22:42:26: 2000000 INFO @ Mon, 29 Jun 2020 22:42:31: 3000000 INFO @ Mon, 29 Jun 2020 22:42:36: 4000000 INFO @ Mon, 29 Jun 2020 22:42:40: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:42:45: 6000000 INFO @ Mon, 29 Jun 2020 22:42:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:42:46: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:42:46: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:42:50: 7000000 INFO @ Mon, 29 Jun 2020 22:42:52: 1000000 INFO @ Mon, 29 Jun 2020 22:42:55: 8000000 INFO @ Mon, 29 Jun 2020 22:42:57: 2000000 INFO @ Mon, 29 Jun 2020 22:43:00: 9000000 INFO @ Mon, 29 Jun 2020 22:43:03: 3000000 INFO @ Mon, 29 Jun 2020 22:43:05: 10000000 INFO @ Mon, 29 Jun 2020 22:43:09: 4000000 INFO @ Mon, 29 Jun 2020 22:43:10: 11000000 BedGraph に変換中... INFO @ Mon, 29 Jun 2020 22:43:14: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:43:15: 12000000 INFO @ Mon, 29 Jun 2020 22:43:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:43:16: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:43:16: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:43:20: 6000000 INFO @ Mon, 29 Jun 2020 22:43:20: 13000000 INFO @ Mon, 29 Jun 2020 22:43:21: 1000000 INFO @ Mon, 29 Jun 2020 22:43:25: 14000000 INFO @ Mon, 29 Jun 2020 22:43:26: 7000000 INFO @ Mon, 29 Jun 2020 22:43:26: 2000000 INFO @ Mon, 29 Jun 2020 22:43:30: 15000000 INFO @ Mon, 29 Jun 2020 22:43:31: 3000000 INFO @ Mon, 29 Jun 2020 22:43:32: 8000000 INFO @ Mon, 29 Jun 2020 22:43:35: 16000000 INFO @ Mon, 29 Jun 2020 22:43:36: 4000000 INFO @ Mon, 29 Jun 2020 22:43:37: 9000000 INFO @ Mon, 29 Jun 2020 22:43:40: 17000000 INFO @ Mon, 29 Jun 2020 22:43:41: 5000000 INFO @ Mon, 29 Jun 2020 22:43:43: 10000000 INFO @ Mon, 29 Jun 2020 22:43:46: 18000000 INFO @ Mon, 29 Jun 2020 22:43:46: 6000000 INFO @ Mon, 29 Jun 2020 22:43:49: 11000000 INFO @ Mon, 29 Jun 2020 22:43:51: 19000000 INFO @ Mon, 29 Jun 2020 22:43:52: 7000000 INFO @ Mon, 29 Jun 2020 22:43:54: 12000000 INFO @ Mon, 29 Jun 2020 22:43:56: 20000000 INFO @ Mon, 29 Jun 2020 22:43:57: 8000000 INFO @ Mon, 29 Jun 2020 22:44:00: 13000000 INFO @ Mon, 29 Jun 2020 22:44:01: 21000000 INFO @ Mon, 29 Jun 2020 22:44:02: 9000000 INFO @ Mon, 29 Jun 2020 22:44:05: 14000000 INFO @ Mon, 29 Jun 2020 22:44:06: 22000000 INFO @ Mon, 29 Jun 2020 22:44:07: 10000000 INFO @ Mon, 29 Jun 2020 22:44:11: 15000000 INFO @ Mon, 29 Jun 2020 22:44:11: 23000000 INFO @ Mon, 29 Jun 2020 22:44:12: 11000000 INFO @ Mon, 29 Jun 2020 22:44:16: 24000000 INFO @ Mon, 29 Jun 2020 22:44:17: 12000000 INFO @ Mon, 29 Jun 2020 22:44:17: 16000000 INFO @ Mon, 29 Jun 2020 22:44:21: 25000000 INFO @ Mon, 29 Jun 2020 22:44:22: 13000000 INFO @ Mon, 29 Jun 2020 22:44:23: 17000000 INFO @ Mon, 29 Jun 2020 22:44:26: 26000000 INFO @ Mon, 29 Jun 2020 22:44:27: 14000000 INFO @ Mon, 29 Jun 2020 22:44:29: 18000000 INFO @ Mon, 29 Jun 2020 22:44:31: 27000000 INFO @ Mon, 29 Jun 2020 22:44:32: 15000000 INFO @ Mon, 29 Jun 2020 22:44:35: 19000000 INFO @ Mon, 29 Jun 2020 22:44:36: 28000000 INFO @ Mon, 29 Jun 2020 22:44:37: 16000000 INFO @ Mon, 29 Jun 2020 22:44:41: 20000000 INFO @ Mon, 29 Jun 2020 22:44:41: 29000000 INFO @ Mon, 29 Jun 2020 22:44:42: 17000000 INFO @ Mon, 29 Jun 2020 22:44:46: 30000000 INFO @ Mon, 29 Jun 2020 22:44:47: 21000000 INFO @ Mon, 29 Jun 2020 22:44:48: 18000000 INFO @ Mon, 29 Jun 2020 22:44:52: 31000000 INFO @ Mon, 29 Jun 2020 22:44:53: 19000000 INFO @ Mon, 29 Jun 2020 22:44:53: 22000000 INFO @ Mon, 29 Jun 2020 22:44:57: 32000000 INFO @ Mon, 29 Jun 2020 22:44:58: 20000000 INFO @ Mon, 29 Jun 2020 22:44:59: 23000000 INFO @ Mon, 29 Jun 2020 22:45:02: 33000000 INFO @ Mon, 29 Jun 2020 22:45:03: 21000000 INFO @ Mon, 29 Jun 2020 22:45:05: 24000000 INFO @ Mon, 29 Jun 2020 22:45:07: 34000000 INFO @ Mon, 29 Jun 2020 22:45:08: 22000000 INFO @ Mon, 29 Jun 2020 22:45:10: 25000000 INFO @ Mon, 29 Jun 2020 22:45:13: 35000000 INFO @ Mon, 29 Jun 2020 22:45:13: 23000000 INFO @ Mon, 29 Jun 2020 22:45:16: 26000000 INFO @ Mon, 29 Jun 2020 22:45:18: 36000000 INFO @ Mon, 29 Jun 2020 22:45:18: 24000000 INFO @ Mon, 29 Jun 2020 22:45:22: 27000000 INFO @ Mon, 29 Jun 2020 22:45:23: 37000000 INFO @ Mon, 29 Jun 2020 22:45:23: 25000000 INFO @ Mon, 29 Jun 2020 22:45:28: 28000000 INFO @ Mon, 29 Jun 2020 22:45:28: 38000000 INFO @ Mon, 29 Jun 2020 22:45:28: 26000000 INFO @ Mon, 29 Jun 2020 22:45:33: 39000000 INFO @ Mon, 29 Jun 2020 22:45:33: 27000000 INFO @ Mon, 29 Jun 2020 22:45:34: 29000000 INFO @ Mon, 29 Jun 2020 22:45:38: 40000000 INFO @ Mon, 29 Jun 2020 22:45:38: 28000000 INFO @ Mon, 29 Jun 2020 22:45:40: 30000000 INFO @ Mon, 29 Jun 2020 22:45:43: 41000000 INFO @ Mon, 29 Jun 2020 22:45:44: 29000000 INFO @ Mon, 29 Jun 2020 22:45:45: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:45:45: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:45:45: #1 total tags in treatment: 41294452 INFO @ Mon, 29 Jun 2020 22:45:45: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:45:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:45:46: 31000000 INFO @ Mon, 29 Jun 2020 22:45:46: #1 tags after filtering in treatment: 41294452 INFO @ Mon, 29 Jun 2020 22:45:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:45:46: #1 finished! INFO @ Mon, 29 Jun 2020 22:45:46: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:45:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:45:48: #2 number of paired peaks: 40 WARNING @ Mon, 29 Jun 2020 22:45:48: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:45:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:45:49: 30000000 INFO @ Mon, 29 Jun 2020 22:45:52: 32000000 INFO @ Mon, 29 Jun 2020 22:45:54: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:45:58: 33000000 INFO @ Mon, 29 Jun 2020 22:45:59: 32000000 INFO @ Mon, 29 Jun 2020 22:46:04: 34000000 INFO @ Mon, 29 Jun 2020 22:46:04: 33000000 INFO @ Mon, 29 Jun 2020 22:46:09: 34000000 INFO @ Mon, 29 Jun 2020 22:46:10: 35000000 INFO @ Mon, 29 Jun 2020 22:46:14: 35000000 INFO @ Mon, 29 Jun 2020 22:46:16: 36000000 INFO @ Mon, 29 Jun 2020 22:46:20: 36000000 INFO @ Mon, 29 Jun 2020 22:46:22: 37000000 INFO @ Mon, 29 Jun 2020 22:46:25: 37000000 INFO @ Mon, 29 Jun 2020 22:46:27: 38000000 INFO @ Mon, 29 Jun 2020 22:46:30: 38000000 INFO @ Mon, 29 Jun 2020 22:46:33: 39000000 INFO @ Mon, 29 Jun 2020 22:46:35: 39000000 INFO @ Mon, 29 Jun 2020 22:46:39: 40000000 INFO @ Mon, 29 Jun 2020 22:46:40: 40000000 INFO @ Mon, 29 Jun 2020 22:46:45: 41000000 INFO @ Mon, 29 Jun 2020 22:46:45: 41000000 INFO @ Mon, 29 Jun 2020 22:46:47: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:46:47: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:46:47: #1 total tags in treatment: 41294452 INFO @ Mon, 29 Jun 2020 22:46:47: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:46:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:46:47: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:46:47: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:46:47: #1 total tags in treatment: 41294452 INFO @ Mon, 29 Jun 2020 22:46:47: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:46:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:46:47: #1 tags after filtering in treatment: 41294452 INFO @ Mon, 29 Jun 2020 22:46:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:46:47: #1 finished! INFO @ Mon, 29 Jun 2020 22:46:47: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:46:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:46:48: #1 tags after filtering in treatment: 41294452 INFO @ Mon, 29 Jun 2020 22:46:48: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:46:48: #1 finished! INFO @ Mon, 29 Jun 2020 22:46:48: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:46:48: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:46:50: #2 number of paired peaks: 40 WARNING @ Mon, 29 Jun 2020 22:46:50: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:46:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:46:50: #2 number of paired peaks: 40 WARNING @ Mon, 29 Jun 2020 22:46:50: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:46:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX159167/SRX159167.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。