Job ID = 9158516 sra ファイルのダウンロード中... Completed: 1030915K bytes transferred in 11 seconds (730727K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 31326472 spots for /home/okishinya/chipatlas/results/dm3/SRX1537618/SRR3109313.sra Written 31326472 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:47 31326472 reads; of these: 31326472 (100.00%) were unpaired; of these: 753399 (2.40%) aligned 0 times 21084393 (67.31%) aligned exactly 1 time 9488680 (30.29%) aligned >1 times 97.60% overall alignment rate Time searching: 00:14:47 Overall time: 00:14:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3596567 / 30573073 = 0.1176 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 17:36:55: # Command line: callpeak -t SRX1537618.bam -f BAM -g dm -n SRX1537618.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1537618.20 # format = BAM # ChIP-seq file = ['SRX1537618.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:36:55: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:36:55: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:36:55: # Command line: callpeak -t SRX1537618.bam -f BAM -g dm -n SRX1537618.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1537618.05 # format = BAM # ChIP-seq file = ['SRX1537618.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:36:55: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:36:55: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:36:55: # Command line: callpeak -t SRX1537618.bam -f BAM -g dm -n SRX1537618.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1537618.10 # format = BAM # ChIP-seq file = ['SRX1537618.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 17:36:55: #1 read tag files... INFO @ Tue, 27 Jun 2017 17:36:55: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 17:37:04: 1000000 INFO @ Tue, 27 Jun 2017 17:37:04: 1000000 INFO @ Tue, 27 Jun 2017 17:37:04: 1000000 INFO @ Tue, 27 Jun 2017 17:37:13: 2000000 INFO @ Tue, 27 Jun 2017 17:37:13: 2000000 INFO @ Tue, 27 Jun 2017 17:37:13: 2000000 INFO @ Tue, 27 Jun 2017 17:37:22: 3000000 INFO @ Tue, 27 Jun 2017 17:37:22: 3000000 INFO @ Tue, 27 Jun 2017 17:37:22: 3000000 INFO @ Tue, 27 Jun 2017 17:37:31: 4000000 INFO @ Tue, 27 Jun 2017 17:37:31: 4000000 INFO @ Tue, 27 Jun 2017 17:37:31: 4000000 INFO @ Tue, 27 Jun 2017 17:37:40: 5000000 INFO @ Tue, 27 Jun 2017 17:37:40: 5000000 INFO @ Tue, 27 Jun 2017 17:37:40: 5000000 INFO @ Tue, 27 Jun 2017 17:37:49: 6000000 INFO @ Tue, 27 Jun 2017 17:37:49: 6000000 INFO @ Tue, 27 Jun 2017 17:37:49: 6000000 INFO @ Tue, 27 Jun 2017 17:37:58: 7000000 INFO @ Tue, 27 Jun 2017 17:37:58: 7000000 INFO @ Tue, 27 Jun 2017 17:37:58: 7000000 INFO @ Tue, 27 Jun 2017 17:38:07: 8000000 INFO @ Tue, 27 Jun 2017 17:38:07: 8000000 INFO @ Tue, 27 Jun 2017 17:38:07: 8000000 INFO @ Tue, 27 Jun 2017 17:38:16: 9000000 INFO @ Tue, 27 Jun 2017 17:38:16: 9000000 INFO @ Tue, 27 Jun 2017 17:38:16: 9000000 INFO @ Tue, 27 Jun 2017 17:38:25: 10000000 INFO @ Tue, 27 Jun 2017 17:38:25: 10000000 INFO @ Tue, 27 Jun 2017 17:38:25: 10000000 INFO @ Tue, 27 Jun 2017 17:38:35: 11000000 INFO @ Tue, 27 Jun 2017 17:38:35: 11000000 INFO @ Tue, 27 Jun 2017 17:38:35: 11000000 INFO @ Tue, 27 Jun 2017 17:38:44: 12000000 INFO @ Tue, 27 Jun 2017 17:38:44: 12000000 INFO @ Tue, 27 Jun 2017 17:38:44: 12000000 INFO @ Tue, 27 Jun 2017 17:38:52: 13000000 INFO @ Tue, 27 Jun 2017 17:38:52: 13000000 INFO @ Tue, 27 Jun 2017 17:38:52: 13000000 INFO @ Tue, 27 Jun 2017 17:39:01: 14000000 INFO @ Tue, 27 Jun 2017 17:39:01: 14000000 INFO @ Tue, 27 Jun 2017 17:39:01: 14000000 INFO @ Tue, 27 Jun 2017 17:39:10: 15000000 INFO @ Tue, 27 Jun 2017 17:39:10: 15000000 INFO @ Tue, 27 Jun 2017 17:39:10: 15000000 INFO @ Tue, 27 Jun 2017 17:39:19: 16000000 INFO @ Tue, 27 Jun 2017 17:39:19: 16000000 INFO @ Tue, 27 Jun 2017 17:39:19: 16000000 INFO @ Tue, 27 Jun 2017 17:39:27: 17000000 INFO @ Tue, 27 Jun 2017 17:39:27: 17000000 INFO @ Tue, 27 Jun 2017 17:39:27: 17000000 INFO @ Tue, 27 Jun 2017 17:39:36: 18000000 INFO @ Tue, 27 Jun 2017 17:39:37: 18000000 INFO @ Tue, 27 Jun 2017 17:39:37: 18000000 INFO @ Tue, 27 Jun 2017 17:39:46: 19000000 INFO @ Tue, 27 Jun 2017 17:39:46: 19000000 INFO @ Tue, 27 Jun 2017 17:39:48: 19000000 INFO @ Tue, 27 Jun 2017 17:39:55: 20000000 INFO @ Tue, 27 Jun 2017 17:39:55: 20000000 INFO @ Tue, 27 Jun 2017 17:40:00: 20000000 INFO @ Tue, 27 Jun 2017 17:40:04: 21000000 INFO @ Tue, 27 Jun 2017 17:40:05: 21000000 INFO @ Tue, 27 Jun 2017 17:40:10: 21000000 INFO @ Tue, 27 Jun 2017 17:40:14: 22000000 INFO @ Tue, 27 Jun 2017 17:40:14: 22000000 INFO @ Tue, 27 Jun 2017 17:40:19: 22000000 INFO @ Tue, 27 Jun 2017 17:40:24: 23000000 INFO @ Tue, 27 Jun 2017 17:40:24: 23000000 INFO @ Tue, 27 Jun 2017 17:40:29: 23000000 INFO @ Tue, 27 Jun 2017 17:40:34: 24000000 INFO @ Tue, 27 Jun 2017 17:40:34: 24000000 INFO @ Tue, 27 Jun 2017 17:40:39: 24000000 INFO @ Tue, 27 Jun 2017 17:40:43: 25000000 INFO @ Tue, 27 Jun 2017 17:40:43: 25000000 INFO @ Tue, 27 Jun 2017 17:40:48: 25000000 INFO @ Tue, 27 Jun 2017 17:40:53: 26000000 INFO @ Tue, 27 Jun 2017 17:40:53: 26000000 INFO @ Tue, 27 Jun 2017 17:40:57: 26000000 INFO @ Tue, 27 Jun 2017 17:41:02: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 17:41:02: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 17:41:02: #1 total tags in treatment: 26976506 INFO @ Tue, 27 Jun 2017 17:41:02: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:41:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:41:02: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 17:41:02: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 17:41:02: #1 total tags in treatment: 26976506 INFO @ Tue, 27 Jun 2017 17:41:02: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:41:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:41:02: #1 tags after filtering in treatment: 26976506 INFO @ Tue, 27 Jun 2017 17:41:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:41:02: #1 finished! INFO @ Tue, 27 Jun 2017 17:41:02: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:41:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:41:02: #1 tags after filtering in treatment: 26976506 INFO @ Tue, 27 Jun 2017 17:41:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:41:02: #1 finished! INFO @ Tue, 27 Jun 2017 17:41:02: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:41:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:41:04: #2 number of paired peaks: 25 WARNING @ Tue, 27 Jun 2017 17:41:04: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:41:04: Process for pairing-model is terminated! cat: SRX1537618.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1537618.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1537618.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1537618.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:41:04: #2 number of paired peaks: 25 WARNING @ Tue, 27 Jun 2017 17:41:04: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:41:04: Process for pairing-model is terminated! cat: SRX1537618.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1537618.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1537618.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1537618.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 17:41:06: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 17:41:06: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 17:41:06: #1 total tags in treatment: 26976506 INFO @ Tue, 27 Jun 2017 17:41:06: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 17:41:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 17:41:07: #1 tags after filtering in treatment: 26976506 INFO @ Tue, 27 Jun 2017 17:41:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 17:41:07: #1 finished! INFO @ Tue, 27 Jun 2017 17:41:07: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 17:41:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 17:41:08: #2 number of paired peaks: 25 WARNING @ Tue, 27 Jun 2017 17:41:08: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 17:41:08: Process for pairing-model is terminated! cat: SRX1537618.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1537618.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1537618.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1537618.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。