Job ID = 6527622 SRX = SRX1531764 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:17:35 prefetch.2.10.7: 1) Downloading 'SRR3102822'... 2020-06-29T13:17:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:20:27 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:20:27 prefetch.2.10.7: 1) 'SRR3102822' was downloaded successfully Read 21069300 spots for SRR3102822/SRR3102822.sra Written 21069300 spots for SRR3102822/SRR3102822.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:43 21069300 reads; of these: 21069300 (100.00%) were unpaired; of these: 990423 (4.70%) aligned 0 times 15916873 (75.55%) aligned exactly 1 time 4162004 (19.75%) aligned >1 times 95.30% overall alignment rate Time searching: 00:06:43 Overall time: 00:06:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2600109 / 20078877 = 0.1295 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:38:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:38:52: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:38:52: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:38:57: 1000000 INFO @ Mon, 29 Jun 2020 22:39:03: 2000000 INFO @ Mon, 29 Jun 2020 22:39:08: 3000000 INFO @ Mon, 29 Jun 2020 22:39:14: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:39:19: 5000000 INFO @ Mon, 29 Jun 2020 22:39:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:39:21: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:39:21: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:39:25: 6000000 INFO @ Mon, 29 Jun 2020 22:39:27: 1000000 INFO @ Mon, 29 Jun 2020 22:39:31: 7000000 INFO @ Mon, 29 Jun 2020 22:39:33: 2000000 INFO @ Mon, 29 Jun 2020 22:39:37: 8000000 INFO @ Mon, 29 Jun 2020 22:39:39: 3000000 INFO @ Mon, 29 Jun 2020 22:39:43: 9000000 INFO @ Mon, 29 Jun 2020 22:39:45: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:39:49: 10000000 INFO @ Mon, 29 Jun 2020 22:39:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:39:51: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:39:51: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:39:52: 5000000 INFO @ Mon, 29 Jun 2020 22:39:55: 11000000 INFO @ Mon, 29 Jun 2020 22:39:57: 1000000 INFO @ Mon, 29 Jun 2020 22:39:58: 6000000 INFO @ Mon, 29 Jun 2020 22:40:01: 12000000 INFO @ Mon, 29 Jun 2020 22:40:03: 2000000 INFO @ Mon, 29 Jun 2020 22:40:04: 7000000 INFO @ Mon, 29 Jun 2020 22:40:07: 13000000 INFO @ Mon, 29 Jun 2020 22:40:09: 3000000 INFO @ Mon, 29 Jun 2020 22:40:09: 8000000 INFO @ Mon, 29 Jun 2020 22:40:13: 14000000 INFO @ Mon, 29 Jun 2020 22:40:15: 4000000 INFO @ Mon, 29 Jun 2020 22:40:15: 9000000 INFO @ Mon, 29 Jun 2020 22:40:19: 15000000 INFO @ Mon, 29 Jun 2020 22:40:21: 5000000 INFO @ Mon, 29 Jun 2020 22:40:21: 10000000 INFO @ Mon, 29 Jun 2020 22:40:25: 16000000 INFO @ Mon, 29 Jun 2020 22:40:27: 6000000 INFO @ Mon, 29 Jun 2020 22:40:27: 11000000 INFO @ Mon, 29 Jun 2020 22:40:30: 17000000 INFO @ Mon, 29 Jun 2020 22:40:33: 7000000 INFO @ Mon, 29 Jun 2020 22:40:33: 12000000 INFO @ Mon, 29 Jun 2020 22:40:33: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 22:40:33: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 22:40:33: #1 total tags in treatment: 17478768 INFO @ Mon, 29 Jun 2020 22:40:33: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:40:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:40:34: #1 tags after filtering in treatment: 17478768 INFO @ Mon, 29 Jun 2020 22:40:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:40:34: #1 finished! INFO @ Mon, 29 Jun 2020 22:40:34: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:40:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:40:35: #2 number of paired peaks: 88 WARNING @ Mon, 29 Jun 2020 22:40:35: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:40:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:40:38: 8000000 INFO @ Mon, 29 Jun 2020 22:40:39: 13000000 INFO @ Mon, 29 Jun 2020 22:40:44: 9000000 INFO @ Mon, 29 Jun 2020 22:40:44: 14000000 INFO @ Mon, 29 Jun 2020 22:40:50: 10000000 INFO @ Mon, 29 Jun 2020 22:40:50: 15000000 INFO @ Mon, 29 Jun 2020 22:40:56: 11000000 INFO @ Mon, 29 Jun 2020 22:40:57: 16000000 INFO @ Mon, 29 Jun 2020 22:41:01: 12000000 INFO @ Mon, 29 Jun 2020 22:41:03: 17000000 INFO @ Mon, 29 Jun 2020 22:41:06: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 22:41:06: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 22:41:06: #1 total tags in treatment: 17478768 INFO @ Mon, 29 Jun 2020 22:41:06: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:41:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:41:06: #1 tags after filtering in treatment: 17478768 INFO @ Mon, 29 Jun 2020 22:41:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:41:06: #1 finished! INFO @ Mon, 29 Jun 2020 22:41:06: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:41:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:41:07: 13000000 INFO @ Mon, 29 Jun 2020 22:41:07: #2 number of paired peaks: 88 WARNING @ Mon, 29 Jun 2020 22:41:07: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:41:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:41:12: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:41:18: 15000000 INFO @ Mon, 29 Jun 2020 22:41:23: 16000000 INFO @ Mon, 29 Jun 2020 22:41:29: 17000000 INFO @ Mon, 29 Jun 2020 22:41:31: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 22:41:31: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 22:41:31: #1 total tags in treatment: 17478768 INFO @ Mon, 29 Jun 2020 22:41:31: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:41:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:41:32: #1 tags after filtering in treatment: 17478768 INFO @ Mon, 29 Jun 2020 22:41:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:41:32: #1 finished! INFO @ Mon, 29 Jun 2020 22:41:32: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:41:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:41:33: #2 number of paired peaks: 88 WARNING @ Mon, 29 Jun 2020 22:41:33: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:41:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1531764/SRX1531764.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。