Job ID = 16439422 SRX = SRX15312099 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T06:07:19 prefetch.2.10.7: 1) Downloading 'SRR19251484'... 2022-08-02T06:07:19 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T06:08:33 prefetch.2.10.7: HTTPS download succeed 2022-08-02T06:08:33 prefetch.2.10.7: 1) 'SRR19251484' was downloaded successfully 2022-08-02T06:08:33 prefetch.2.10.7: 'SRR19251484' has 0 unresolved dependencies Read 42656944 spots for SRR19251484/SRR19251484.sra Written 42656944 spots for SRR19251484/SRR19251484.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439596 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:03 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:14 42656944 reads; of these: 42656944 (100.00%) were unpaired; of these: 947887 (2.22%) aligned 0 times 32110107 (75.28%) aligned exactly 1 time 9598950 (22.50%) aligned >1 times 97.78% overall alignment rate Time searching: 00:21:17 Overall time: 00:21:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4619471 / 41709057 = 0.1108 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:49:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:49:46: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:49:46: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:49:57: 1000000 INFO @ Tue, 02 Aug 2022 15:50:05: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:50:13: 3000000 INFO @ Tue, 02 Aug 2022 15:50:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:50:16: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:50:16: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:50:21: 4000000 INFO @ Tue, 02 Aug 2022 15:50:25: 1000000 INFO @ Tue, 02 Aug 2022 15:50:30: 5000000 INFO @ Tue, 02 Aug 2022 15:50:34: 2000000 INFO @ Tue, 02 Aug 2022 15:50:39: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:50:43: 3000000 INFO @ Tue, 02 Aug 2022 15:50:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:50:45: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:50:45: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:50:48: 7000000 INFO @ Tue, 02 Aug 2022 15:50:52: 4000000 INFO @ Tue, 02 Aug 2022 15:50:56: 1000000 INFO @ Tue, 02 Aug 2022 15:50:57: 8000000 INFO @ Tue, 02 Aug 2022 15:51:00: 5000000 INFO @ Tue, 02 Aug 2022 15:51:06: 9000000 INFO @ Tue, 02 Aug 2022 15:51:07: 2000000 INFO @ Tue, 02 Aug 2022 15:51:09: 6000000 INFO @ Tue, 02 Aug 2022 15:51:14: 10000000 INFO @ Tue, 02 Aug 2022 15:51:18: 3000000 INFO @ Tue, 02 Aug 2022 15:51:18: 7000000 INFO @ Tue, 02 Aug 2022 15:51:23: 11000000 INFO @ Tue, 02 Aug 2022 15:51:27: 8000000 INFO @ Tue, 02 Aug 2022 15:51:28: 4000000 INFO @ Tue, 02 Aug 2022 15:51:32: 12000000 INFO @ Tue, 02 Aug 2022 15:51:36: 9000000 INFO @ Tue, 02 Aug 2022 15:51:38: 5000000 INFO @ Tue, 02 Aug 2022 15:51:42: 13000000 INFO @ Tue, 02 Aug 2022 15:51:45: 10000000 INFO @ Tue, 02 Aug 2022 15:51:49: 6000000 INFO @ Tue, 02 Aug 2022 15:51:51: 14000000 INFO @ Tue, 02 Aug 2022 15:51:53: 11000000 INFO @ Tue, 02 Aug 2022 15:51:59: 7000000 INFO @ Tue, 02 Aug 2022 15:52:00: 15000000 INFO @ Tue, 02 Aug 2022 15:52:02: 12000000 INFO @ Tue, 02 Aug 2022 15:52:09: 16000000 INFO @ Tue, 02 Aug 2022 15:52:09: 8000000 INFO @ Tue, 02 Aug 2022 15:52:11: 13000000 INFO @ Tue, 02 Aug 2022 15:52:18: 17000000 INFO @ Tue, 02 Aug 2022 15:52:20: 9000000 INFO @ Tue, 02 Aug 2022 15:52:20: 14000000 INFO @ Tue, 02 Aug 2022 15:52:26: 18000000 INFO @ Tue, 02 Aug 2022 15:52:28: 15000000 INFO @ Tue, 02 Aug 2022 15:52:30: 10000000 INFO @ Tue, 02 Aug 2022 15:52:35: 19000000 INFO @ Tue, 02 Aug 2022 15:52:37: 16000000 INFO @ Tue, 02 Aug 2022 15:52:42: 11000000 INFO @ Tue, 02 Aug 2022 15:52:44: 20000000 INFO @ Tue, 02 Aug 2022 15:52:46: 17000000 INFO @ Tue, 02 Aug 2022 15:52:52: 12000000 INFO @ Tue, 02 Aug 2022 15:52:53: 21000000 INFO @ Tue, 02 Aug 2022 15:52:55: 18000000 INFO @ Tue, 02 Aug 2022 15:53:02: 22000000 INFO @ Tue, 02 Aug 2022 15:53:03: 13000000 INFO @ Tue, 02 Aug 2022 15:53:05: 19000000 INFO @ Tue, 02 Aug 2022 15:53:11: 23000000 INFO @ Tue, 02 Aug 2022 15:53:13: 20000000 INFO @ Tue, 02 Aug 2022 15:53:14: 14000000 INFO @ Tue, 02 Aug 2022 15:53:20: 24000000 INFO @ Tue, 02 Aug 2022 15:53:22: 21000000 INFO @ Tue, 02 Aug 2022 15:53:24: 15000000 INFO @ Tue, 02 Aug 2022 15:53:29: 25000000 INFO @ Tue, 02 Aug 2022 15:53:31: 22000000 INFO @ Tue, 02 Aug 2022 15:53:35: 16000000 INFO @ Tue, 02 Aug 2022 15:53:38: 26000000 INFO @ Tue, 02 Aug 2022 15:53:39: 23000000 INFO @ Tue, 02 Aug 2022 15:53:45: 17000000 INFO @ Tue, 02 Aug 2022 15:53:47: 27000000 INFO @ Tue, 02 Aug 2022 15:53:48: 24000000 INFO @ Tue, 02 Aug 2022 15:53:56: 28000000 INFO @ Tue, 02 Aug 2022 15:53:56: 18000000 INFO @ Tue, 02 Aug 2022 15:53:57: 25000000 INFO @ Tue, 02 Aug 2022 15:54:05: 29000000 INFO @ Tue, 02 Aug 2022 15:54:06: 26000000 INFO @ Tue, 02 Aug 2022 15:54:07: 19000000 INFO @ Tue, 02 Aug 2022 15:54:14: 30000000 INFO @ Tue, 02 Aug 2022 15:54:15: 27000000 INFO @ Tue, 02 Aug 2022 15:54:17: 20000000 INFO @ Tue, 02 Aug 2022 15:54:23: 31000000 INFO @ Tue, 02 Aug 2022 15:54:25: 28000000 INFO @ Tue, 02 Aug 2022 15:54:28: 21000000 INFO @ Tue, 02 Aug 2022 15:54:32: 32000000 INFO @ Tue, 02 Aug 2022 15:54:34: 29000000 INFO @ Tue, 02 Aug 2022 15:54:39: 22000000 INFO @ Tue, 02 Aug 2022 15:54:41: 33000000 INFO @ Tue, 02 Aug 2022 15:54:43: 30000000 INFO @ Tue, 02 Aug 2022 15:54:49: 34000000 INFO @ Tue, 02 Aug 2022 15:54:50: 23000000 INFO @ Tue, 02 Aug 2022 15:54:51: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:54:58: 35000000 INFO @ Tue, 02 Aug 2022 15:55:00: 32000000 INFO @ Tue, 02 Aug 2022 15:55:01: 24000000 INFO @ Tue, 02 Aug 2022 15:55:08: 36000000 INFO @ Tue, 02 Aug 2022 15:55:08: 33000000 INFO @ Tue, 02 Aug 2022 15:55:11: 25000000 INFO @ Tue, 02 Aug 2022 15:55:16: 37000000 INFO @ Tue, 02 Aug 2022 15:55:17: 34000000 INFO @ Tue, 02 Aug 2022 15:55:18: #1 tag size is determined as 72 bps INFO @ Tue, 02 Aug 2022 15:55:18: #1 tag size = 72 INFO @ Tue, 02 Aug 2022 15:55:18: #1 total tags in treatment: 37089586 INFO @ Tue, 02 Aug 2022 15:55:18: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:55:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:55:18: #1 tags after filtering in treatment: 37089586 INFO @ Tue, 02 Aug 2022 15:55:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:55:18: #1 finished! INFO @ Tue, 02 Aug 2022 15:55:18: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:55:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:55:21: #2 number of paired peaks: 23 WARNING @ Tue, 02 Aug 2022 15:55:21: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:55:21: Process for pairing-model is terminated! INFO @ Tue, 02 Aug 2022 15:55:22: 26000000 cut: /home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:55:25: 35000000 INFO @ Tue, 02 Aug 2022 15:55:32: 27000000 INFO @ Tue, 02 Aug 2022 15:55:33: 36000000 INFO @ Tue, 02 Aug 2022 15:55:41: 37000000 INFO @ Tue, 02 Aug 2022 15:55:43: #1 tag size is determined as 72 bps INFO @ Tue, 02 Aug 2022 15:55:43: #1 tag size = 72 INFO @ Tue, 02 Aug 2022 15:55:43: #1 total tags in treatment: 37089586 INFO @ Tue, 02 Aug 2022 15:55:43: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:55:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:55:43: 28000000 INFO @ Tue, 02 Aug 2022 15:55:44: #1 tags after filtering in treatment: 37089586 INFO @ Tue, 02 Aug 2022 15:55:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:55:44: #1 finished! INFO @ Tue, 02 Aug 2022 15:55:44: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:55:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:55:47: #2 number of paired peaks: 23 WARNING @ Tue, 02 Aug 2022 15:55:47: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:55:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:55:53: 29000000 INFO @ Tue, 02 Aug 2022 15:56:03: 30000000 INFO @ Tue, 02 Aug 2022 15:56:15: 31000000 INFO @ Tue, 02 Aug 2022 15:56:26: 32000000 INFO @ Tue, 02 Aug 2022 15:56:37: 33000000 INFO @ Tue, 02 Aug 2022 15:56:49: 34000000 INFO @ Tue, 02 Aug 2022 15:57:00: 35000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:57:10: 36000000 INFO @ Tue, 02 Aug 2022 15:57:21: 37000000 INFO @ Tue, 02 Aug 2022 15:57:23: #1 tag size is determined as 72 bps INFO @ Tue, 02 Aug 2022 15:57:23: #1 tag size = 72 INFO @ Tue, 02 Aug 2022 15:57:23: #1 total tags in treatment: 37089586 INFO @ Tue, 02 Aug 2022 15:57:23: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:57:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:57:23: #1 tags after filtering in treatment: 37089586 INFO @ Tue, 02 Aug 2022 15:57:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:57:24: #1 finished! INFO @ Tue, 02 Aug 2022 15:57:24: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:57:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:57:26: #2 number of paired peaks: 23 WARNING @ Tue, 02 Aug 2022 15:57:26: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:57:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312099/SRX15312099.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling