Job ID = 16439421 SRX = SRX15312098 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T06:06:49 prefetch.2.10.7: 1) Downloading 'SRR19251485'... 2022-08-02T06:06:49 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T06:07:51 prefetch.2.10.7: HTTPS download succeed 2022-08-02T06:07:51 prefetch.2.10.7: 1) 'SRR19251485' was downloaded successfully 2022-08-02T06:07:51 prefetch.2.10.7: 'SRR19251485' has 0 unresolved dependencies Read 31492210 spots for SRR19251485/SRR19251485.sra Written 31492210 spots for SRR19251485/SRR19251485.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439522 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:43 31492210 reads; of these: 31492210 (100.00%) were unpaired; of these: 1207239 (3.83%) aligned 0 times 24970403 (79.29%) aligned exactly 1 time 5314568 (16.88%) aligned >1 times 96.17% overall alignment rate Time searching: 00:13:44 Overall time: 00:13:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3437127 / 30284971 = 0.1135 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:36:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:36:45: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:36:45: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:36:54: 1000000 INFO @ Tue, 02 Aug 2022 15:37:03: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:37:12: 3000000 INFO @ Tue, 02 Aug 2022 15:37:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:37:16: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:37:16: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:37:20: 4000000 INFO @ Tue, 02 Aug 2022 15:37:24: 1000000 INFO @ Tue, 02 Aug 2022 15:37:30: 5000000 INFO @ Tue, 02 Aug 2022 15:37:32: 2000000 INFO @ Tue, 02 Aug 2022 15:37:39: 3000000 INFO @ Tue, 02 Aug 2022 15:37:40: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:37:46: 4000000 INFO @ Tue, 02 Aug 2022 15:37:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:37:47: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:37:47: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:37:50: 7000000 INFO @ Tue, 02 Aug 2022 15:37:54: 5000000 INFO @ Tue, 02 Aug 2022 15:37:55: 1000000 INFO @ Tue, 02 Aug 2022 15:37:59: 8000000 INFO @ Tue, 02 Aug 2022 15:38:02: 6000000 INFO @ Tue, 02 Aug 2022 15:38:02: 2000000 INFO @ Tue, 02 Aug 2022 15:38:08: 9000000 INFO @ Tue, 02 Aug 2022 15:38:09: 7000000 INFO @ Tue, 02 Aug 2022 15:38:10: 3000000 INFO @ Tue, 02 Aug 2022 15:38:18: 8000000 INFO @ Tue, 02 Aug 2022 15:38:18: 4000000 INFO @ Tue, 02 Aug 2022 15:38:19: 10000000 INFO @ Tue, 02 Aug 2022 15:38:26: 9000000 INFO @ Tue, 02 Aug 2022 15:38:27: 5000000 INFO @ Tue, 02 Aug 2022 15:38:29: 11000000 INFO @ Tue, 02 Aug 2022 15:38:34: 10000000 INFO @ Tue, 02 Aug 2022 15:38:35: 6000000 INFO @ Tue, 02 Aug 2022 15:38:39: 12000000 INFO @ Tue, 02 Aug 2022 15:38:42: 11000000 INFO @ Tue, 02 Aug 2022 15:38:42: 7000000 INFO @ Tue, 02 Aug 2022 15:38:48: 13000000 INFO @ Tue, 02 Aug 2022 15:38:50: 8000000 INFO @ Tue, 02 Aug 2022 15:38:51: 12000000 INFO @ Tue, 02 Aug 2022 15:38:57: 14000000 INFO @ Tue, 02 Aug 2022 15:38:58: 9000000 INFO @ Tue, 02 Aug 2022 15:39:00: 13000000 INFO @ Tue, 02 Aug 2022 15:39:07: 15000000 INFO @ Tue, 02 Aug 2022 15:39:06: 10000000 INFO @ Tue, 02 Aug 2022 15:39:08: 14000000 INFO @ Tue, 02 Aug 2022 15:39:15: 11000000 INFO @ Tue, 02 Aug 2022 15:39:16: 15000000 INFO @ Tue, 02 Aug 2022 15:39:17: 16000000 INFO @ Tue, 02 Aug 2022 15:39:23: 12000000 INFO @ Tue, 02 Aug 2022 15:39:24: 16000000 INFO @ Tue, 02 Aug 2022 15:39:26: 17000000 INFO @ Tue, 02 Aug 2022 15:39:31: 13000000 INFO @ Tue, 02 Aug 2022 15:39:32: 17000000 INFO @ Tue, 02 Aug 2022 15:39:37: 18000000 INFO @ Tue, 02 Aug 2022 15:39:40: 14000000 INFO @ Tue, 02 Aug 2022 15:39:40: 18000000 INFO @ Tue, 02 Aug 2022 15:39:47: 19000000 INFO @ Tue, 02 Aug 2022 15:39:48: 15000000 INFO @ Tue, 02 Aug 2022 15:39:48: 19000000 INFO @ Tue, 02 Aug 2022 15:39:56: 16000000 INFO @ Tue, 02 Aug 2022 15:39:56: 20000000 INFO @ Tue, 02 Aug 2022 15:39:58: 20000000 INFO @ Tue, 02 Aug 2022 15:40:04: 17000000 INFO @ Tue, 02 Aug 2022 15:40:06: 21000000 INFO @ Tue, 02 Aug 2022 15:40:09: 21000000 INFO @ Tue, 02 Aug 2022 15:40:13: 18000000 INFO @ Tue, 02 Aug 2022 15:40:15: 22000000 INFO @ Tue, 02 Aug 2022 15:40:20: 22000000 INFO @ Tue, 02 Aug 2022 15:40:21: 19000000 INFO @ Tue, 02 Aug 2022 15:40:23: 23000000 INFO @ Tue, 02 Aug 2022 15:40:29: 20000000 INFO @ Tue, 02 Aug 2022 15:40:29: 23000000 INFO @ Tue, 02 Aug 2022 15:40:32: 24000000 INFO @ Tue, 02 Aug 2022 15:40:37: 21000000 INFO @ Tue, 02 Aug 2022 15:40:39: 24000000 INFO @ Tue, 02 Aug 2022 15:40:40: 25000000 INFO @ Tue, 02 Aug 2022 15:40:45: 22000000 INFO @ Tue, 02 Aug 2022 15:40:48: 26000000 INFO @ Tue, 02 Aug 2022 15:40:49: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:40:54: 23000000 INFO @ Tue, 02 Aug 2022 15:40:56: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:40:56: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:40:56: #1 total tags in treatment: 26847844 INFO @ Tue, 02 Aug 2022 15:40:56: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:40:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:40:57: #1 tags after filtering in treatment: 26847844 INFO @ Tue, 02 Aug 2022 15:40:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:40:57: #1 finished! INFO @ Tue, 02 Aug 2022 15:40:57: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:40:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:40:58: 26000000 INFO @ Tue, 02 Aug 2022 15:40:59: #2 number of paired peaks: 61 WARNING @ Tue, 02 Aug 2022 15:40:59: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:40:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:41:02: 24000000 INFO @ Tue, 02 Aug 2022 15:41:07: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:41:07: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:41:07: #1 total tags in treatment: 26847844 INFO @ Tue, 02 Aug 2022 15:41:07: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:41:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:41:07: #1 tags after filtering in treatment: 26847844 INFO @ Tue, 02 Aug 2022 15:41:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:41:08: #1 finished! INFO @ Tue, 02 Aug 2022 15:41:08: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:41:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:41:09: 25000000 INFO @ Tue, 02 Aug 2022 15:41:10: #2 number of paired peaks: 61 WARNING @ Tue, 02 Aug 2022 15:41:10: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:41:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:41:16: 26000000 INFO @ Tue, 02 Aug 2022 15:41:23: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:41:23: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:41:23: #1 total tags in treatment: 26847844 INFO @ Tue, 02 Aug 2022 15:41:23: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:41:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:41:24: #1 tags after filtering in treatment: 26847844 INFO @ Tue, 02 Aug 2022 15:41:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:41:24: #1 finished! INFO @ Tue, 02 Aug 2022 15:41:24: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:41:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:41:26: #2 number of paired peaks: 61 WARNING @ Tue, 02 Aug 2022 15:41:26: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:41:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312098/SRX15312098.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。