Job ID = 16439420 SRX = SRX15312097 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T06:06:48 prefetch.2.10.7: 1) Downloading 'SRR19251486'... 2022-08-02T06:06:48 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T06:08:26 prefetch.2.10.7: HTTPS download succeed 2022-08-02T06:08:26 prefetch.2.10.7: 1) 'SRR19251486' was downloaded successfully 2022-08-02T06:08:26 prefetch.2.10.7: 'SRR19251486' has 0 unresolved dependencies Read 43781975 spots for SRR19251486/SRR19251486.sra Written 43781975 spots for SRR19251486/SRR19251486.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439539 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:46 43781975 reads; of these: 43781975 (100.00%) were unpaired; of these: 1303440 (2.98%) aligned 0 times 33686513 (76.94%) aligned exactly 1 time 8792022 (20.08%) aligned >1 times 97.02% overall alignment rate Time searching: 00:16:46 Overall time: 00:16:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5258343 / 42478535 = 0.1238 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:38:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:38:54: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:38:54: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:39:03: 1000000 INFO @ Tue, 02 Aug 2022 15:39:12: 2000000 INFO @ Tue, 02 Aug 2022 15:39:20: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:39:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:39:24: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:39:24: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:39:30: 4000000 INFO @ Tue, 02 Aug 2022 15:39:35: 1000000 INFO @ Tue, 02 Aug 2022 15:39:40: 5000000 INFO @ Tue, 02 Aug 2022 15:39:45: 2000000 INFO @ Tue, 02 Aug 2022 15:39:50: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:39:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:39:54: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:39:54: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:39:55: 3000000 INFO @ Tue, 02 Aug 2022 15:40:00: 7000000 INFO @ Tue, 02 Aug 2022 15:40:05: 1000000 INFO @ Tue, 02 Aug 2022 15:40:06: 4000000 INFO @ Tue, 02 Aug 2022 15:40:10: 8000000 INFO @ Tue, 02 Aug 2022 15:40:16: 2000000 INFO @ Tue, 02 Aug 2022 15:40:16: 5000000 INFO @ Tue, 02 Aug 2022 15:40:21: 9000000 INFO @ Tue, 02 Aug 2022 15:40:26: 3000000 INFO @ Tue, 02 Aug 2022 15:40:27: 6000000 INFO @ Tue, 02 Aug 2022 15:40:32: 10000000 INFO @ Tue, 02 Aug 2022 15:40:37: 7000000 INFO @ Tue, 02 Aug 2022 15:40:38: 4000000 INFO @ Tue, 02 Aug 2022 15:40:42: 11000000 INFO @ Tue, 02 Aug 2022 15:40:48: 8000000 INFO @ Tue, 02 Aug 2022 15:40:49: 5000000 INFO @ Tue, 02 Aug 2022 15:40:53: 12000000 INFO @ Tue, 02 Aug 2022 15:40:58: 9000000 INFO @ Tue, 02 Aug 2022 15:40:59: 6000000 INFO @ Tue, 02 Aug 2022 15:41:03: 13000000 INFO @ Tue, 02 Aug 2022 15:41:09: 10000000 INFO @ Tue, 02 Aug 2022 15:41:10: 7000000 INFO @ Tue, 02 Aug 2022 15:41:14: 14000000 INFO @ Tue, 02 Aug 2022 15:41:19: 11000000 INFO @ Tue, 02 Aug 2022 15:41:21: 8000000 INFO @ Tue, 02 Aug 2022 15:41:24: 15000000 INFO @ Tue, 02 Aug 2022 15:41:29: 12000000 INFO @ Tue, 02 Aug 2022 15:41:32: 9000000 INFO @ Tue, 02 Aug 2022 15:41:35: 16000000 INFO @ Tue, 02 Aug 2022 15:41:39: 13000000 INFO @ Tue, 02 Aug 2022 15:41:43: 10000000 INFO @ Tue, 02 Aug 2022 15:41:45: 17000000 INFO @ Tue, 02 Aug 2022 15:41:49: 14000000 INFO @ Tue, 02 Aug 2022 15:41:53: 11000000 INFO @ Tue, 02 Aug 2022 15:41:55: 18000000 INFO @ Tue, 02 Aug 2022 15:41:59: 15000000 INFO @ Tue, 02 Aug 2022 15:42:04: 12000000 INFO @ Tue, 02 Aug 2022 15:42:05: 19000000 INFO @ Tue, 02 Aug 2022 15:42:08: 16000000 INFO @ Tue, 02 Aug 2022 15:42:14: 13000000 INFO @ Tue, 02 Aug 2022 15:42:15: 20000000 INFO @ Tue, 02 Aug 2022 15:42:18: 17000000 INFO @ Tue, 02 Aug 2022 15:42:24: 14000000 INFO @ Tue, 02 Aug 2022 15:42:24: 21000000 INFO @ Tue, 02 Aug 2022 15:42:28: 18000000 INFO @ Tue, 02 Aug 2022 15:42:33: 15000000 INFO @ Tue, 02 Aug 2022 15:42:34: 22000000 INFO @ Tue, 02 Aug 2022 15:42:38: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:42:43: 16000000 INFO @ Tue, 02 Aug 2022 15:42:43: 23000000 INFO @ Tue, 02 Aug 2022 15:42:47: 20000000 INFO @ Tue, 02 Aug 2022 15:42:53: 17000000 INFO @ Tue, 02 Aug 2022 15:42:53: 24000000 INFO @ Tue, 02 Aug 2022 15:42:57: 21000000 INFO @ Tue, 02 Aug 2022 15:43:02: 25000000 INFO @ Tue, 02 Aug 2022 15:43:02: 18000000 INFO @ Tue, 02 Aug 2022 15:43:07: 22000000 INFO @ Tue, 02 Aug 2022 15:43:11: 26000000 INFO @ Tue, 02 Aug 2022 15:43:12: 19000000 INFO @ Tue, 02 Aug 2022 15:43:17: 23000000 INFO @ Tue, 02 Aug 2022 15:43:21: 27000000 INFO @ Tue, 02 Aug 2022 15:43:21: 20000000 INFO @ Tue, 02 Aug 2022 15:43:27: 24000000 INFO @ Tue, 02 Aug 2022 15:43:30: 28000000 INFO @ Tue, 02 Aug 2022 15:43:31: 21000000 INFO @ Tue, 02 Aug 2022 15:43:37: 25000000 INFO @ Tue, 02 Aug 2022 15:43:39: 29000000 INFO @ Tue, 02 Aug 2022 15:43:40: 22000000 INFO @ Tue, 02 Aug 2022 15:43:47: 26000000 INFO @ Tue, 02 Aug 2022 15:43:49: 30000000 INFO @ Tue, 02 Aug 2022 15:43:49: 23000000 INFO @ Tue, 02 Aug 2022 15:43:57: 27000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:43:58: 31000000 INFO @ Tue, 02 Aug 2022 15:43:58: 24000000 INFO @ Tue, 02 Aug 2022 15:44:06: 28000000 INFO @ Tue, 02 Aug 2022 15:44:07: 32000000 INFO @ Tue, 02 Aug 2022 15:44:08: 25000000 INFO @ Tue, 02 Aug 2022 15:44:16: 29000000 INFO @ Tue, 02 Aug 2022 15:44:17: 26000000 INFO @ Tue, 02 Aug 2022 15:44:17: 33000000 INFO @ Tue, 02 Aug 2022 15:44:25: 30000000 INFO @ Tue, 02 Aug 2022 15:44:26: 34000000 INFO @ Tue, 02 Aug 2022 15:44:26: 27000000 INFO @ Tue, 02 Aug 2022 15:44:35: 31000000 INFO @ Tue, 02 Aug 2022 15:44:35: 35000000 INFO @ Tue, 02 Aug 2022 15:44:35: 28000000 INFO @ Tue, 02 Aug 2022 15:44:44: 36000000 INFO @ Tue, 02 Aug 2022 15:44:44: 32000000 INFO @ Tue, 02 Aug 2022 15:44:45: 29000000 INFO @ Tue, 02 Aug 2022 15:44:53: 37000000 INFO @ Tue, 02 Aug 2022 15:44:54: 33000000 INFO @ Tue, 02 Aug 2022 15:44:55: 30000000 INFO @ Tue, 02 Aug 2022 15:44:55: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:44:55: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:44:55: #1 total tags in treatment: 37220192 INFO @ Tue, 02 Aug 2022 15:44:55: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:44:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:44:56: #1 tags after filtering in treatment: 37220192 INFO @ Tue, 02 Aug 2022 15:44:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:44:56: #1 finished! INFO @ Tue, 02 Aug 2022 15:44:56: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:44:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:44:58: #2 number of paired peaks: 11 WARNING @ Tue, 02 Aug 2022 15:44:58: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:44:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:45:02: 34000000 INFO @ Tue, 02 Aug 2022 15:45:03: 31000000 INFO @ Tue, 02 Aug 2022 15:45:11: 35000000 INFO @ Tue, 02 Aug 2022 15:45:11: 32000000 INFO @ Tue, 02 Aug 2022 15:45:19: 36000000 INFO @ Tue, 02 Aug 2022 15:45:20: 33000000 INFO @ Tue, 02 Aug 2022 15:45:27: 37000000 INFO @ Tue, 02 Aug 2022 15:45:28: 34000000 INFO @ Tue, 02 Aug 2022 15:45:29: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:45:29: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:45:29: #1 total tags in treatment: 37220192 INFO @ Tue, 02 Aug 2022 15:45:29: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:45:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:45:30: #1 tags after filtering in treatment: 37220192 INFO @ Tue, 02 Aug 2022 15:45:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:45:30: #1 finished! INFO @ Tue, 02 Aug 2022 15:45:30: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:45:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:45:32: #2 number of paired peaks: 11 WARNING @ Tue, 02 Aug 2022 15:45:32: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:45:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:45:36: 35000000 INFO @ Tue, 02 Aug 2022 15:45:44: 36000000 INFO @ Tue, 02 Aug 2022 15:45:52: 37000000 INFO @ Tue, 02 Aug 2022 15:45:54: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 15:45:54: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 15:45:54: #1 total tags in treatment: 37220192 INFO @ Tue, 02 Aug 2022 15:45:54: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:45:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:45:54: #1 tags after filtering in treatment: 37220192 INFO @ Tue, 02 Aug 2022 15:45:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:45:54: #1 finished! INFO @ Tue, 02 Aug 2022 15:45:54: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:45:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:45:57: #2 number of paired peaks: 11 WARNING @ Tue, 02 Aug 2022 15:45:57: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:45:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX15312097/SRX15312097.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling